WO2011144257A1 - Amorphous resorbable polymeric network materials with shape memory - Google Patents

Amorphous resorbable polymeric network materials with shape memory Download PDF

Info

Publication number
WO2011144257A1
WO2011144257A1 PCT/EP2010/057075 EP2010057075W WO2011144257A1 WO 2011144257 A1 WO2011144257 A1 WO 2011144257A1 EP 2010057075 W EP2010057075 W EP 2010057075W WO 2011144257 A1 WO2011144257 A1 WO 2011144257A1
Authority
WO
WIPO (PCT)
Prior art keywords
shape memory
polymeric network
network materials
resorbable polymeric
memory according
Prior art date
Application number
PCT/EP2010/057075
Other languages
French (fr)
Inventor
Dirk Wybe Grijpma
Shahriar Sharifi
Original Assignee
Rijksuniversiteit Groningen
Academisch Ziekenhuis Groningen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rijksuniversiteit Groningen, Academisch Ziekenhuis Groningen filed Critical Rijksuniversiteit Groningen
Priority to PCT/EP2010/057075 priority Critical patent/WO2011144257A1/en
Publication of WO2011144257A1 publication Critical patent/WO2011144257A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F299/00—Macromolecular compounds obtained by interreacting polymers involving only carbon-to-carbon unsaturated bond reactions, in the absence of non-macromolecular monomers
    • C08F299/02—Macromolecular compounds obtained by interreacting polymers involving only carbon-to-carbon unsaturated bond reactions, in the absence of non-macromolecular monomers from unsaturated polycondensates
    • C08F299/04—Macromolecular compounds obtained by interreacting polymers involving only carbon-to-carbon unsaturated bond reactions, in the absence of non-macromolecular monomers from unsaturated polycondensates from polyesters
    • C08F299/0407—Processes of polymerisation
    • C08F299/0421—Polymerisation initiated by wave energy or particle radiation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/58—Materials at least partially resorbable by the body
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F299/00—Macromolecular compounds obtained by interreacting polymers involving only carbon-to-carbon unsaturated bond reactions, in the absence of non-macromolecular monomers
    • C08F299/02—Macromolecular compounds obtained by interreacting polymers involving only carbon-to-carbon unsaturated bond reactions, in the absence of non-macromolecular monomers from unsaturated polycondensates
    • C08F299/04—Macromolecular compounds obtained by interreacting polymers involving only carbon-to-carbon unsaturated bond reactions, in the absence of non-macromolecular monomers from unsaturated polycondensates from polyesters
    • C08F299/0485—Macromolecular compounds obtained by interreacting polymers involving only carbon-to-carbon unsaturated bond reactions, in the absence of non-macromolecular monomers from unsaturated polycondensates from polyesters from polyesters with side or terminal unsaturations
    • C08F299/0492—Macromolecular compounds obtained by interreacting polymers involving only carbon-to-carbon unsaturated bond reactions, in the absence of non-macromolecular monomers from unsaturated polycondensates from polyesters from polyesters with side or terminal unsaturations the unsaturation being in acrylic or methacrylic groups
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2400/00—Materials characterised by their function or physical properties
    • A61L2400/16—Materials with shape-memory or superelastic properties
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L2201/00—Properties
    • C08L2201/12—Shape memory

Definitions

  • the present invention relates to methods for preparing amorphous resorbable polymer network materials with shape memory. Further, the present invention relates to the amorphous resorbable polymeric network materials with shape memory and to the use of the present amorphous
  • scaffolds engineered extracellular matrices
  • cells and biologically active molecules.
  • the physical, chemical and biological properties of these scaffold materials are important in tissue engineering. Cell behaviour is important in tissue engineering.
  • the scaffold should transmit mechanical stimuli to the cells and tissues, and withstand repeated dynamic loadings in vivo. Also in vitro this can be required, as mechanical stimulation of cell-seeded tissue engineering scaffolds during cell culture enhances the development of engineered tissues and their function.
  • Resorbable polymer networks prepared from amorphous oligomers with low glass transition temperatures can for example be based on trimethylene carbonate (TMC) and/or D,L-lactide (DLLA) oligomers, and show elastomeric
  • (meth) acrylate- or fumarate groups yields to functionalized oligomers, or macromers, amongst others, to allow network formation .
  • poly (D, L-lactide) (PDLLA) is known as biodegradable, thermoplastic, aliphatic polyester derived from renewable resources, such as corn starch or sugarcanes.
  • PDLLA can be used in medical applications, such as sutures, stents, dialysis media and drug delivery devices. It is also being evaluated as a material for tissue engineering. Accordingly, the racemic lactide monomer D,L- lactide (DLLA) is suitable in the methods of the present invention.
  • the toughness, ultimate tensile strengths and elongation at break values of flexible networks obtained in this manner significantly increase with increasing macromer molecular weights and molecular weights between crosslinks.
  • Shape memory polymeric materials are materials that have the ability to pass from one deformed state (temporary shape) to another state (permanent shape) induced by an external stimulus (trigger) , such as a temperature or pH change, a water content modification, an electric or magnetic field, or light.
  • an external stimulus such as a temperature or pH change, a water content modification, an electric or magnetic field, or light.
  • polymers in general they also cover a wide range of materials with properties ranging from stable to biodegradable, from soft to hard, from hydrophilic to hydrophobic and from elastic to rigid, depending on the structural units that constitute to their structure. They can be thermoplastics and/or thermoset
  • inventions which comprise: a) the preparation of polyol oligomers with a number average molecular weight higher than or equal to 900 g/mol per hydroxy-group, by polymerization of cyclic carbonates and/or cyclic esters and/or linear carbonates and/or linear esters and/or hydroxycarboxylic acids using an initiator; b) the functionalization of the polyol oligomers with at least two unsaturated groups; c) the crosslinking of the functionalized oligomers obtained in step b) using an initiator.
  • Polymeric network materials also designated as polymer networks or polymer materials and also called polymers in the context of the present invention, are large molecules composed of repeating structural units,
  • Amorphous polymeric network materials are polymers whose molecular structure lacks a definite repeating form, shape, or crystalline structure.
  • Amorphous resorbable polymeric network materials are polymers that are resorbable, at least substantially.
  • Resorbable materials of the present invention can also be designated by degradable or biodegradable materials.
  • the amorphous resorbable polymeric network materials of the present invention are not harmful to the human or animal body (biocompatible) , and are therefore compatible with medical applications in the body, where degradation of the networks can take place by hydrolysis and/or oxidation.
  • An oligomer is a polymer with a low degree of polymerization.
  • a functionalized oligomer is also designated as a macromer.
  • a polyol is a hydrocarbon containing several hydroxy-groups , also called alcohol functional group, -OH.
  • a diol contains two -OH group
  • a triol contains three -OH groups
  • a tetra-ol contains four -OH group
  • a penta-ol contains five -OH groups.
  • Esters are hydrocarbons with at least one ester group -COO- in the hydrocarbon, such as one ester group, two ester groups, three ester groups.
  • Hydroxycarboxylic acids are hydrocarbons with a carboxylic acids (organic function -C00H) comprising a second organic group, a hydroxy group (-0H) .
  • Cyclic molecules have a ring structure, such as aryl, or aromatic rings.
  • Linear molecules are hydrocarbon chains, such as alkanes.
  • An unsaturated group is an alkenyl or alkynyl, hydrocarbons with a double or a triple C-C bond.
  • unsaturated group can bind at the terminal or at any combination
  • An alkenyl is a rest comprising at least one double C-C bond and an alkynyl is a rest comprising at least one triple C-C bond.
  • Step b) of the methods of the present invention comprises at least two unsaturated group to functionalize each oligomer, such as two unsaturated groups or more, three unsaturated groups or more, four unsaturated groups or more, five unsaturated groups or more.
  • An initiator is a chemical substance that can start the polymerization, such as by producing radical or ionic species under mild conditions.
  • An initiator has an active site, such as an active hydrogen to allow the polymerization to process.
  • the initiator allows a ring-opening polymerization or a polycondensation .
  • the initiator in step c) is a radical forming initiator.
  • the initiator in step a) and step c) may be the same initiator. In the present invention, the number average
  • molecular weight is used to define the size of the polyol oligomers, and accordingly to characterize the polymeric network materials.
  • the number- average molecular weight is determined experimentally by methods that count the number of polymer molecules in a sample of the polymer and is defined as the total weight of all the molecules in a polymer sample divided by the total number of moles present.
  • the number average molecular weight is commonly designated M n .
  • the number average molecular weight per hydroxy- group of the polyol oligomer is higher than, or equal to 1000 g/mol, preferably higher than, or equal to 1500 g/mol, more preferably higher than, or equal to 2000 g/mol, most preferably higher than, or equal to 3000 g/mol.
  • the number average molecular weight per hydroxy-group of the polyol oligomer is equal to, or lower than, 500000 g/mol,
  • the number average molecular weight per hydroxy- group of the polyol oligomer is in the range of 1000 to 150000 g/mol, preferably 1500 to 100000 g/mol, more
  • the functionalization in step b) can be end- functionalization or co-polymerization.
  • End- functionalization is the functionalization of the polyol oligomers carried out with an unsaturated group (double or triple bond at one extremity of the hydrocarbon chain) .
  • Co- polymerization is polymerization, for example polycondensation, of mixtures of different monomers and leads to polymeric products with (two or more) different structures in the polymer chain.
  • Such mixture can be a mixture of monomers that contain double C-C bonds and monomers that do not contain double C-C bonds.
  • the polyol oligomers are chosen from the group diols, triols, tetra-ols or penta-ols.
  • Other polyols are also of interest, such as sugar alcohols (such as maltitol,
  • sorbitol xylitol and isomalt
  • polyethylene glycol sorbitol, xylitol and isomalt
  • Polyethylene glycol is a polyether compound with many applications from industrial manufacturing to medicine. It has also been known historically as polyethylene oxide (PEO) or polyoxyethylene (POE) . PEG, PEO or POE refers to an oligomer or polymer of ethylene oxide. Polyethylene glycol is produced by the interaction of ethylene oxide with water, ethylene glycol or ethylene glycol oligomers. The reaction is catalyzed by acidic or basic catalysts. It is the basis of a number of laxatives (e.g., macrogol-containing
  • Sterilization by autoclaving can be done with amorphous poly ( lactides ) and PCL-PEO co-polymers when they are crosslinked.
  • the cyclic carbonates are chosen from the group trimethylene carbonate, ethylene carbonate, propylene carbonate,
  • diethylene glycol-bis- allyl-carbonate and derivatives thereof diethylene glycol-bis- allyl-carbonate and derivatives thereof .
  • Trimethylene carbonate has the formula (CH2) 2OCH 2 C0 2 .
  • Ethylene carbonate with the formula is also called 1,3- dioxolan-2-one .
  • Ethylene carbonate and propylene carbonate are esters of ethylene glycol or propylene glycol,
  • Diethylene glycol-bis- allyl-carbonate has the formula C 12 H 18 O 7 .
  • derivatives thereof is to be understood as substituted trimethylene carbonate, substituted ethylene carbonate, substituted propylene carbonate, substituted diethylene glycol-bis- allyl-carbonate which can be
  • alkyl substituted at any position, by an alkyl, alkenyl, alkynyl, aryl, cycloalkyl, cycloalkenyl , cycloalkynyl .
  • substituent may carry or be an organic group.
  • the cyclic esters are chosen from the group lactones, D,L- lactide, glycolide, and derivatives thereof.
  • Lactones are cyclic esters. The smaller (according to the size of their cycle) are -acetolactone (three- membered ring with the formula (CH 2 )C0 2 ), ⁇ - propiolactone ( four—membered ring with the formula (01 2 ) 2 C0 2 ) , ⁇ -butyrolactone ( five-membered ring with the formula
  • caprolactone ( seven-membered ring with the formula (CH 2 )sC0 2 ) .
  • D,L-lactide has de formula C 6 H 8 C>4.
  • Glycolide 1 4-dioxane-2 , 5- dione and has the formula C 4 H 4 O 4 .
  • derivatives thereof is to be understood as substituted lactones, substituted D,L- lactides, substituted glycolide which can be substituted at any position, by an alkyl, alkenyl, alkynyl, aryl,
  • cycloalkyl cycloalkenyl, cycloalkynyl.
  • the substituent may carry or be an organic group.
  • the linear carbonates are chosen from the group diethyl carbonate or diphenylcarbonate and derivatives thereof.
  • Diethyl carbonate has the formula C 5 H 10 O 3 .
  • Diphenylcarbonate has the formula Ci 3 H 10 O 3 .
  • the linear esters are chosen from the group fumaric acid monoethyl ester, fumaric acid diethylester ,
  • Fumaric acid diethylester, or diethyl fumarate has the formula C 8 H 12 0 4 .
  • Dimethylterephtalate, or terephthalic acid methyl ester has the formula Ci 0 H 10 O 4 .
  • Diethylterephtalate, or terephthalic acid ethyl ester has the formula Ci 2 H 14 0 4 .
  • the substituent may carry or be an organic group .
  • Oligomers comprising a double or triple bond at the end of the main chain can be obtain by ring-opening
  • Oligomers with a double or triple bond at the end of the main chain provide amorphous resorbable polymeric network materials with increased strength and toughness .
  • the linear hydroxycarboxilic acids are any linear carboxylic acids comprising a hydroxyl group on the main hydrocarbon chain, such as -hydroxy acids. They can be saturated or unsaturated (comprising double and or triple bonds in the carbon chain), and/or substituted or unsubst ituted by alkyl, alkenyl or alkynyl. Hydroxy carboxylic acids, are a class of chemical compounds that consist of a carboxylic acid and a hydroxyl group. They may be either naturally occurring or synthetic. AHAs are well-known for their use in the cosmetics industry. A carboxylic group is -COOH, an hydroxy group is the organic alcohol function -OH.
  • AHAs are most commonly used in cosmetic applications are typically derived from food products including glycolic acid (from sugar cane) , lactic acid (from sour milk) , malic acid (from apples) , citric acid (from citrus fruits) and tartaric acid (from grape wine) .
  • the polymerization in step a) is a ring-opening
  • Ring-opening polymerization is a form of
  • an initiator e.g. comprising an active hydrogen as active site
  • a catalyst brings about cleavage of the ring followed by polymerization to yield oligomers or polymers.
  • the reaction is commonly carried out under inert conditions (argon, nitrogen or vacuum) and under heating.
  • Polycondensation is a process by which two or more molecules join together, resulting in loss of small
  • the type of end product resulting from a condensation polymerization is dependent on the number of functional end groups of the monomer which can react .
  • the unsaturated end group is chosen from the group acrylate, methacrylate, fumarate, diacrylate, triacrylate. Other acrylates are also of interest.
  • the acrylate unit comprises one vinyl group and one ester group.
  • a diacrylate contains two units of acrylate, a triacrylate contains three units of acrylate.
  • the acrylate unit is also called propenoate and has the formula C 3 H 3 O 2 .
  • Examples of acrylates are methacrylate, also called 2- methylprop-2-enoate with the linear formula CsH 8 0 2 , ethylene diacrylate with the formula C 8 H 10 O 4 , pentaerythritol
  • PETA triacrylate
  • the unsaturated end group can be substituted, e.g. by a rest alkyl or acyl .
  • the crosslinking in step c) is photocrosslinking, thermal crosslinking or redox crosslinking.
  • Crosslinking means that the polymeric network materials of the invention have bonds that link polymer chains in order to form a network. These networks of polymers are also called crosslinked polymers.
  • crosslinking allowed by a change in temperature. Redox crosslinking occurs via an electrochemical reaction. Other crosslinking reactions are also of interest such as
  • Irradiation is the process by which an item is exposed to radiation.
  • the radiation of the present invention is ultraviolet light (the ultraviolet region has a
  • wavelength between 1 and 400 nm preferably at wavelengths from 250 nm to 400 nm, more preferably 300 nm to 380nm, or visible light (400 nm to 850 nm) , or any other form of radiation including microwave irradiation.
  • the initiator in step a) is a diol, a triol, a tetra-ol, a diamine, a triamine, tetra-amine, dithiol, trithiol or a tetra-thiol.
  • a diol, triol or tetra-ol has two, three or four organic alcoholic functions -OH (also called hydroxy group), respectively.
  • a diamine, triamine or tetra-amine has two, three or four amine groups -NH- or -NH 2 , respectively.
  • a dithiol, trithiol or tetrathiol has two, three or four -SH groups, respectively.
  • the initiator in step a) is chosen from the group 1 , 2-ethanediol , 1 , 3-propanediol , 1 , 4-butanediol , 1,5- pentanediol, 1 , 6-hexanediol , 1 , 8-octanediol .
  • the initiator concentration in step a) is in the range 10 ⁇ 6 to 10 ⁇ 2 mol per total mol of monomers.
  • step a) comprises a catalyst.
  • a catalyst is a chemical substance able to
  • step a) comprises a catalyst which is a stannous alkyloate or a metallic compound chosen from the group stannous octoate or metallic zinc.
  • Other catalysts are also of interest, such as tin, titanium, iron, zink or calcium based catalysts.
  • the catalyst concentration in step a) is in the range of 10 _i to lCT 2 mol per total mol of the monomers.
  • the polyol oligomers in step a) are prepared from two monomers chosen from the group cyclic carbonates and/or a cyclic esters and/or linear carbonates and/or linear esters and/or hydroxycarboxylic acids with a ratio between monomers which is in the range of 0.01:100 to 100:0.01 by mol.
  • the temperature range in step i) is between 20 ° C to 200 ° C, such as 20 ° C, 25 ° C, 30 ° C, 35 ° C, 40 ° C, 45 ° C, 50 ° C, 55 ° C, 60 ° C, 65 ° C, 70 ° C, 75 ° C, 80 ° C, 85 ° C, 90 ° C, 95 ° C, 100 ° C, 105 ° C, 110 ° C, 115 ° C 120 ° C, 125 ° C, 130 ° C, 135 ° C, 140 ° C, 145 ° C, 150 ° C, 155 ° C, 160 ° C, 165 ° C, 170 ° C, 175 ° C, 180 ° C, 185 ° C, 190 ° C, 195 ° C, 200 ° C.
  • the polymerization reaction in step a) can also be done at different temperature than the temperature at which the monomers are mixed.
  • step iii) is mechanical stirring, ultrasonic mixing or magnetic agitation. Stirring until homogeneity is stirring until forming a uniform mixture to the naked eye.
  • the functionalization is carried out with methacrylic anhydride or methacryloyl chloride and an amine in an organic solvent.
  • the solvent can be a chlorinated organic solvent .
  • Methacrylic anhydride has the formula C 8 H 10 O 3 .
  • Methacryloyl chloride has the formula C 4 H 5 C10.
  • An amine is a chemical group comprising a -N-, the amine can be primary, -NH 2 , secondary -RNH, or tertiary -RNR.
  • a chlorinated organic solvent is a hydrocarbon containing chlorine. Examples are dichloromethane,
  • Dichloromethane has the formula CH 2 C1 2 , chloroform CHC1 3 , carbontetrachloride CC1 4 .
  • the crosslinking in step c) is photocrosslinking with ultraviolet light or visible light.
  • the ultraviolet light used in the present invention has a wavelength between 1 and 400 nm, preferably at wavelengths from 250 nm to 400 nm, more preferably 300 nm to 380nm.
  • the visible light has a wavelength between 400 nm and 850 nm.
  • the initiator of the crosslinking can be a
  • radical/ionic species upon exposure to light or a thermal initiator, a chemical substance that produces the
  • radical / ionic species with sensitivity to temperature or a redox initiator, a chemical substance that produces the radical / ionic species by a redox reaction.
  • the initiator is chosen from the class hydroxyketone, aminoketone, phenylglyoxylate .
  • a phenylglyoxylate has an aromatic C 6 H 6 hydrocarbon and an ester function -COO- .
  • the photoinitiator is 2-hydroxy-4 ' - (2-hydroxyethoxy ) -2- methylpropiophenone .
  • 2-hydroxy-4 ' - (2-hydroxyethoxy) -2- methylpropiophenone has the formula C 12 H 16 O 4 .
  • the methods further comprise a step b2) between step b) and step c) of compression molding or extrusion or injection molding or casting of the functionalized oligomer obtained in step b) .
  • the process of casting can also be solvent casting.
  • Compression molding is a method of molding in which the molding material, generally preheated, is first placed in an open, (heated) mold cavity. The mold is closed with a top force or plug member, pressure is applied to force the material into contact with all mold areas, while heat and pressure are maintained until the molding material has cured. Extrusion is a process used to create objects of a fixed cross-sectional profile. A material is pushed or drawn through a die of the desired cross-section. The main
  • Injection molding is a manufacturing process for producing parts of polymer materials. The material is fed into a heated barrel, mixed, and forced into a mold cavity where it cools and hardens to the configuration of the mold cavity. After a product is designed, molds are made in usually either steel or aluminium, and precision-machined to form the features of the desired part. Injection molding is widely used for manufacturing a variety of parts. Casting is a process for forming thermoplastic articles by dipping a male mold into a liquid polymer, a solution or dispersion of the polymer and drawing off the solvent (if any present) to leave a layer of plastic film adhering to the mold.
  • the shape changes in the polymer network material are induced by stimuli chosen from the group thermal, electric, pH, magnetic stimuli, light or gamma irradiation, or water content modification.
  • amorphous polymeric materials with shape memory are obtainable by the methods of the present invention.
  • the amorphous polymeric network materials with shape memory of the present invention are used for coating surfaces, as a protective layer for thermal insulation and/or for anti-oxidation insulation, for the manufacture of packaging materials.
  • the amorphous polymeric network materials with shape memory of the present invention are used for preparing medical devices.
  • the amorphous polymeric network materials with shape memory are used to prepare implants, used in tissue engineering, or drug delivery.
  • DLLA 0.6
  • TMC 0.4
  • oligomers with number average molecular weight of approximately 30000g/mol displaying shape memory behaviour A) Shape at 10 °C (Temporary shape); B) Shape at 37 °C after 20 s;
  • a series of poly (D, L-lactide-co-trimethylene carbonate) diols and their corresponding homopolymers i.e oligomeric Poly (DLLA) and Poly (TMC) diols, are synthesized by ring opening polymerization of DLLA and TMC using 1 , 6-hexanediol as an initiator and SnOct 2 as a catalyst.
  • the composition in the oligomeric copolymers (TMC:DLLA molar ratio) is varied from 0 to 1 in steps of 0.2.
  • the employed monomer feed compositions and the amounts of initiator are shown in Table 1.
  • the required amounts of TMC or DLLA or their mixtures along with the initiator are charged into a 250 mL three necked round bottom flask equipped with magnetic stirrer under the flow of nitrogen (Table 1) .
  • the number average molecular weight of oligomers can be varied by adjusting the monomer to initiator molar ratio.
  • the flask is then evacuated and purged with nitrogen several times and heated to 130 °C under a nitrogen atmosphere.
  • the synthesized diols are subsequently methacrylate- endcapped.
  • This reaction is carried out using methacrylic anhydride in the presence of triethyl amine (TEA) .
  • TEA triethyl amine
  • the oligomers, methacrylic anhydride, and TEA are measured out in 1:4:4 molar ratios.
  • the pre-dried oligomer is charged into a 3-necked round bottom flask under nitrogen atmosphere containing 100 mL of anhydrous dichloromethane. After dissolution of the oligomer, methacrylic anhydride and TEA are charged into the flask.
  • the reaction is allowed to proceed for 7 days at room temperature. Purification is done by precipitation in cold excess ethanol.
  • the precipitated macromer is then vacuum dried for 2 days at room temperature and stored at -20 °C.
  • Example 2 Example 2
  • the photo-crosslinking process is carried out by UV light (365 nm) using Irgacure 2959 as a photoinitiator .
  • the photoinitiator (2 wt% of the macromer) is dissolved in chloroform (1 mL/g macromer) and then added to the macromer. The mixture is then gently heated and stirred manually until a homogenous mixture is obtained.
  • the solvent is
  • Macromers based on PTMC and PDLLA homopolymeric oligomers and the TMC and DLLA copolymeric oligomers, which form covalently crosslinked networks upon photo-initiated radical polymerization, were prepared by the two step process of example 1.
  • hydroxyl-group terminated TMC and DLLA oligomers using methacrylic anhydride were then crosslinked according to the photo-polymerization process of example 2.
  • Table 2 gives an overview of the properties of the macromers and the obtained networks.
  • Tables 3 to 6 give an overview of the thermal and mechanical properties of the prepared networks.
  • Tg determined by DSC on specimens extracted using dichloromethane b) Tg determined by dynamic mechanical analysis (temperature at which tan5 reaches a maximum value) .
  • the dynamic mechanical thermal properties of extracted photocrosslinked networks measuring 5x20x0.5 mm 3 were determined using a PerkinElmer DMA 7 operating in the tensile mode. Storage modulus and loss tangent values (tan ⁇ ) were determined between -50 °C and 100 °C at a heating rate of 2 °C/min. The static strain was 200 mN. Table 5. Mechanical properties of photo-crosslinked networks prepared from macromers with number average molecular weights of approximately 30000 g/mol after extraction and drying determined by tensile testing at room temperature (23 °C) . Values between parentheses are standard deviations.
  • the mechanical properties of the polymer network materials of the present invention are significantly better than the materials known up to date: the tensile strength and
  • elongation at break values are equal or higher in the polymer network material of the present invention.
  • the amorphous photo-crosslinked networks display shape memory properties. For instance, when a network is prepared from a DLLA ( 0.6 ) : TMC ( 0.4 ) macromer with number average molecular weight of approximately 30000g/mol in the shape of a spring by photo-crosslinking using a transparent mould, the specimen can be deformed to a linear shape by heating the spring to above the glass transition temperature, stretching the specimen and cooling the stretched specimen to below the glass transition temperature. This specimen maintains is temporary shape while it is kept at
  • the shape memory polymers investigated here present an amorphous crosslinked network.
  • Amorphous resorbable networks with glass transition temperatures below or close to body temperature are most suited for biomedical applications where the loading conditions are relatively low.

Abstract

The present invention relates to a method for preparing an amorphous resorbable polymer material with shape memory. Further, the present invention relates to the amorphous resorbable polymer material with shape memory and furthermore to the use of the amorphous resorbable polymer material with shape memory.

Description

AMORPHOUS RESORBABLE POLYMERIC NETWORK MATERIALS WITH SHAPE
MEMORY
The present invention relates to methods for preparing amorphous resorbable polymer network materials with shape memory. Further, the present invention relates to the amorphous resorbable polymeric network materials with shape memory and to the use of the present amorphous
resorbable polymeric network materials with shape memory.
Developments in the multidisciplinary field of tissue engineering have yielded a novel set of tissue replacement parts and implementation strategies. Scientific advances in biomaterials , stem cells, growth and
differentiation factors, and biomimetic environments have created opportunities to synthesize tissues in the
laboratory as well as in the body from combinations of engineered extracellular matrices ("scaffolds"), cells, and biologically active molecules. The physical, chemical and biological properties of these scaffold materials are important in tissue engineering. Cell behaviour is
influenced by the rigidity of the substrate on which the cells are cultured. In soft tissue engineering, the scaffold should transmit mechanical stimuli to the cells and tissues, and withstand repeated dynamic loadings in vivo. Also in vitro this can be required, as mechanical stimulation of cell-seeded tissue engineering scaffolds during cell culture enhances the development of engineered tissues and their function. In this respect, flexible, form-stable and
resorbable elastomeric polymer networks that allow cell adhesion and proliferation are of great interest.
Resorbable polymer networks prepared from amorphous oligomers with low glass transition temperatures (Tgs) , can for example be based on trimethylene carbonate (TMC) and/or D,L-lactide (DLLA) oligomers, and show elastomeric
properties. For example, the end-functionalization of these relatively low molecular weight polymers with
(meth) acrylate- or fumarate groups yields to functionalized oligomers, or macromers, amongst others, to allow network formation .
In the lactide class, poly (D, L-lactide) (PDLLA) is known as biodegradable, thermoplastic, aliphatic polyester derived from renewable resources, such as corn starch or sugarcanes. PDLLA can be used in medical applications, such as sutures, stents, dialysis media and drug delivery devices. It is also being evaluated as a material for tissue engineering. Accordingly, the racemic lactide monomer D,L- lactide (DLLA) is suitable in the methods of the present invention.
The toughness, ultimate tensile strengths and elongation at break values of flexible networks obtained in this manner significantly increase with increasing macromer molecular weights and molecular weights between crosslinks.
Shape memory polymeric materials are materials that have the ability to pass from one deformed state (temporary shape) to another state (permanent shape) induced by an external stimulus (trigger) , such as a temperature or pH change, a water content modification, an electric or magnetic field, or light. As well as polymers in general, they also cover a wide range of materials with properties ranging from stable to biodegradable, from soft to hard, from hydrophilic to hydrophobic and from elastic to rigid, depending on the structural units that constitute to their structure. They can be thermoplastics and/or thermoset
(covalently cross-linked) polymeric materials. They are known to be able to store up to three different shapes. There is a continuous need in the art to further develop amorphous resorbable shape memory polymeric network materials. It is a goal of the present invention, among others, to prepare amorphous shape memory polymeric network materials that are resorbable, at least substantially. The present invention also aims at preparing resorbable flexible polymeric network materials with a glass transition
temperature close to body temperature and with a
sufficiently high molecular weight to have better, or equal, mechanical properties (for example, a better toughness and/or better tensile strength and/or better elongation at break) . This allows the material to be implanted in its temporary shape at low temperatures (room temperature for example) when it is relatively hard and rigid, and when it heats to body temperature becomes more flexible and adopts its permanent shape. By varying the composition of the oligomer (and accordingly the composition of the macromer and network) , we can tune the glass transition temperature of the networks as well as their mechanical properties.
These goals, amongst others, are met by the methods for preparing amorphous resorbable polymeric network
materials with shape memory according to the present
invention, which comprise: a) the preparation of polyol oligomers with a number average molecular weight higher than or equal to 900 g/mol per hydroxy-group, by polymerization of cyclic carbonates and/or cyclic esters and/or linear carbonates and/or linear esters and/or hydroxycarboxylic acids using an initiator; b) the functionalization of the polyol oligomers with at least two unsaturated groups; c) the crosslinking of the functionalized oligomers obtained in step b) using an initiator.
Polymeric network materials, also designated as polymer networks or polymer materials and also called polymers in the context of the present invention, are large molecules composed of repeating structural units,
synthesized from monomers, typically connected by covalent chemical bonds.
Amorphous polymeric network materials are polymers whose molecular structure lacks a definite repeating form, shape, or crystalline structure.
Amorphous resorbable polymeric network materials are polymers that are resorbable, at least substantially.
Resorbable materials of the present invention can also be designated by degradable or biodegradable materials. The amorphous resorbable polymeric network materials of the present invention are not harmful to the human or animal body (biocompatible) , and are therefore compatible with medical applications in the body, where degradation of the networks can take place by hydrolysis and/or oxidation.
These processes can be mediated by enzymes, which can be excreted by cells. It is understood that the polymeric network materials of the present invention maintain their mechanical properties until it is no longer needed and then be degraded and resorbed. Typically, the backbone of the polymer is hydrolytically unstable. That is, the polymer is unstable in a water based environment. The hydrolysis can also be favorized enzymatically .
An oligomer is a polymer with a low degree of polymerization. A functionalized oligomer is also designated as a macromer.
A polyol is a hydrocarbon containing several hydroxy-groups , also called alcohol functional group, -OH. A diol contains two -OH group, a triol contains three -OH groups, a tetra-ol contains four -OH group and a penta-ol contains five -OH groups. Carbonates are hydrocarbons with at least one carbonate group -O-C (=0) -0- in the hydrocarbon, such as one carbonate group, two carbonate groups, three carbonate groups .
Esters are hydrocarbons with at least one ester group -COO- in the hydrocarbon, such as one ester group, two ester groups, three ester groups.
Hydroxycarboxylic acids are hydrocarbons with a carboxylic acids (organic function -C00H) comprising a second organic group, a hydroxy group (-0H) .
Cyclic molecules have a ring structure, such as aryl, or aromatic rings. Linear molecules are hydrocarbon chains, such as alkanes.
An unsaturated group is an alkenyl or alkynyl, hydrocarbons with a double or a triple C-C bond. The
unsaturated group can bind at the terminal or at any
position in the hydrocarbon chain. An alkenyl is a rest comprising at least one double C-C bond and an alkynyl is a rest comprising at least one triple C-C bond.
Step b) of the methods of the present invention comprises at least two unsaturated group to functionalize each oligomer, such as two unsaturated groups or more, three unsaturated groups or more, four unsaturated groups or more, five unsaturated groups or more.
An initiator is a chemical substance that can start the polymerization, such as by producing radical or ionic species under mild conditions. An initiator has an active site, such as an active hydrogen to allow the polymerization to process. In step a) the initiator allows a ring-opening polymerization or a polycondensation . The initiator in step c) is a radical forming initiator. The initiator in step a) and step c) may be the same initiator. In the present invention, the number average
molecular weight, usually expressed in g/mol, is used to define the size of the polyol oligomers, and accordingly to characterize the polymeric network materials. The number- average molecular weight is determined experimentally by methods that count the number of polymer molecules in a sample of the polymer and is defined as the total weight of all the molecules in a polymer sample divided by the total number of moles present. The number average molecular weight is commonly designated Mn .
The number average molecular weight per hydroxy- group of the polyol oligomer is higher than, or equal to 1000 g/mol, preferably higher than, or equal to 1500 g/mol, more preferably higher than, or equal to 2000 g/mol, most preferably higher than, or equal to 3000 g/mol. The number average molecular weight per hydroxy-group of the polyol oligomer is equal to, or lower than, 500000 g/mol,
preferably equal to, or lower than 250000 g/mol, more preferably equal to, or lower than 150000 g/mol, most preferably equal to, or lower than 50000 g/mol.
According to a preferred embodiment of the present invention, the number average molecular weight per hydroxy- group of the polyol oligomer is in the range of 1000 to 150000 g/mol, preferably 1500 to 100000 g/mol, more
preferably 2000 g/mol to 50000 g/mol, most preferably 5000 g/mol to 30000 g/mol.
According to the method of the present invention, the functionalization in step b) can be end- functionalization or co-polymerization. End- functionalization is the functionalization of the polyol oligomers carried out with an unsaturated group (double or triple bond at one extremity of the hydrocarbon chain) . Co- polymerization is polymerization, for example polycondensation, of mixtures of different monomers and leads to polymeric products with (two or more) different structures in the polymer chain. Such mixture can be a mixture of monomers that contain double C-C bonds and monomers that do not contain double C-C bonds.
According to the methods of the present invention, the polyol oligomers are chosen from the group diols, triols, tetra-ols or penta-ols. Other polyols are also of interest, such as sugar alcohols (such as maltitol,
sorbitol, xylitol and isomalt) or polyethylene glycol.
Polyethylene glycol (PEG) is a polyether compound with many applications from industrial manufacturing to medicine. It has also been known historically as polyethylene oxide (PEO) or polyoxyethylene (POE) . PEG, PEO or POE refers to an oligomer or polymer of ethylene oxide. Polyethylene glycol is produced by the interaction of ethylene oxide with water, ethylene glycol or ethylene glycol oligomers. The reaction is catalyzed by acidic or basic catalysts. It is the basis of a number of laxatives (e.g., macrogol-containing
products) and of many skin creams.
Sterilization by autoclaving can be done with amorphous poly ( lactides ) and PCL-PEO co-polymers when they are crosslinked.
According to the methods of the present invention, the cyclic carbonates are chosen from the group trimethylene carbonate, ethylene carbonate, propylene carbonate,
diethylene glycol-bis- allyl-carbonate and derivatives thereof .
Trimethylene carbonate has the formula (CH2) 2OCH2C02. Ethylene carbonate with the formula is also called 1,3- dioxolan-2-one . Ethylene carbonate and propylene carbonate are esters of ethylene glycol or propylene glycol,
respectively, and carbonic acid. They have the formula C3H403 and C4H6O3. Diethylene glycol-bis- allyl-carbonate has the formula C12H18O7. By derivatives thereof is to be understood as substituted trimethylene carbonate, substituted ethylene carbonate, substituted propylene carbonate, substituted diethylene glycol-bis- allyl-carbonate which can be
substituted at any position, by an alkyl, alkenyl, alkynyl, aryl, cycloalkyl, cycloalkenyl , cycloalkynyl . The
substituent may carry or be an organic group.
According to the methods of the present invention, the cyclic esters are chosen from the group lactones, D,L- lactide, glycolide, and derivatives thereof.
Lactones are cyclic esters. The smaller (according to the size of their cycle) are -acetolactone (three- membered ring with the formula (CH2)C02), β- propiolactone ( four—membered ring with the formula (012)2C02) , γ-butyrolactone ( five-membered ring with the formula
(CH2)3C02), and δ-valerolactone ( six-membered ring with the formula (CH2)4CC>2) and ε-caprolactone, also called
caprolactone ( seven-membered ring with the formula (CH2)sC02) . D,L-lactide has de formula C6H8C>4. Glycolide 1 , 4-dioxane-2 , 5- dione and has the formula C4H4O4. By derivatives thereof is to be understood as substituted lactones, substituted D,L- lactides, substituted glycolide which can be substituted at any position, by an alkyl, alkenyl, alkynyl, aryl,
cycloalkyl, cycloalkenyl, cycloalkynyl. The substituent may carry or be an organic group.
According to the methods of the present invention, the linear carbonates are chosen from the group diethyl carbonate or diphenylcarbonate and derivatives thereof.
Diethyl carbonate has the formula C5H10O3. Diphenylcarbonate has the formula Ci3H10O3.
According to the methods of the present invention, the linear esters are chosen from the group fumaric acid monoethyl ester, fumaric acid diethylester ,
dimethylterephtalate, diethylterephtalate and derivatives thereof. Fumaric acid monoethyl ester, or monoethyl
fumarate, has the formula C6H8C>4. Fumaric acid diethylester, or diethyl fumarate, has the formula C8H1204.
Dimethylterephtalate, or terephthalic acid methyl ester, has the formula Ci0H10O4. Diethylterephtalate, or terephthalic acid ethyl ester, has the formula Ci2H1404.
By derivatives thereof is to be understood as substituted linear carbonates, linear esters or linear hydroxycarboxylic monomers at any position, by an alkyl, alkenyl, alkynyl, aryl, cycloalkyl, cycloalkenyl ,
cycloalkynyl . The substituent may carry or be an organic group .
Oligomers comprising a double or triple bond at the end of the main chain can be obtain by ring-opening
polymerization of cyclic carbonates or cyclic esters
containing a pendant double bond, or by polycondensat ion of fumaric acid or derivatives thereof, maleic acid or
derivatives thereof. Oligomers with a double or triple bond at the end of the main chain provide amorphous resorbable polymeric network materials with increased strength and toughness .
According to the methods of the present invention, the linear hydroxycarboxilic acids are any linear carboxylic acids comprising a hydroxyl group on the main hydrocarbon chain, such as -hydroxy acids. They can be saturated or unsaturated (comprising double and or triple bonds in the carbon chain), and/or substituted or unsubst ituted by alkyl, alkenyl or alkynyl. Hydroxy carboxylic acids, are a class of chemical compounds that consist of a carboxylic acid and a hydroxyl group. They may be either naturally occurring or synthetic. AHAs are well-known for their use in the cosmetics industry. A carboxylic group is -COOH, an hydroxy group is the organic alcohol function -OH. -hydroxy acids , or AHAs, are most commonly used in cosmetic applications are typically derived from food products including glycolic acid (from sugar cane) , lactic acid (from sour milk) , malic acid (from apples) , citric acid (from citrus fruits) and tartaric acid (from grape wine) .
According to the methods of the present invention, the polymerization in step a) is a ring-opening
polymerization or polycondensation .
Ring-opening polymerization is a form of
polymerization, in which the terminal end of a polymer acts as a reactive center, where further cyclic monomers join to form a larger polymer chain through propagation. The
treatment of some cyclic compounds with an initiator (e.g. comprising an active hydrogen as active site) and a catalyst brings about cleavage of the ring followed by polymerization to yield oligomers or polymers. The reaction is commonly carried out under inert conditions (argon, nitrogen or vacuum) and under heating.
Polycondensation is a process by which two or more molecules join together, resulting in loss of small
molecules which is often water. The type of end product resulting from a condensation polymerization is dependent on the number of functional end groups of the monomer which can react .
According to the methods of the present invention, the unsaturated end group is chosen from the group acrylate, methacrylate, fumarate, diacrylate, triacrylate. Other acrylates are also of interest.
The acrylate unit comprises one vinyl group and one ester group. A diacrylate contains two units of acrylate, a triacrylate contains three units of acrylate. The acrylate unit is also called propenoate and has the formula C3H3O2. Examples of acrylates are methacrylate, also called 2- methylprop-2-enoate with the linear formula CsH802, ethylene diacrylate with the formula C8H10O4, pentaerythritol
triacrylate (PETA) , also called 2-propenoic acid 2-
(hydroxymethyl ) -2- [ [ ( l-oxo-2-propenyl ) oxy ] methyl ] -1 , 3- propanediyl ester with the linear formula
(H2C=CHC02CH2) 3CCH2OH, trimethylolpropane triacrylate (TMPTA) with the linear formula (H2C=CHC02CH2) 3CC2H5 and
trimethylolpropane trimethacrylate with the linear formula [H2C=C (CH3) C02CH2] 3CC2H5. The unsaturated end group can be substituted, e.g. by a rest alkyl or acyl .
A fumarate has the general formula (02CCH=CHC02) and can be substituted by an alkyl or acyl.
According to the methods of the present invention, the crosslinking in step c) is photocrosslinking, thermal crosslinking or redox crosslinking.
Crosslinking means that the polymeric network materials of the invention have bonds that link polymer chains in order to form a network. These networks of polymers are also called crosslinked polymers.
Photocrosslinking is crosslinking due to
irradiadiation with light. Thermal crosslinking is
crosslinking allowed by a change in temperature. Redox crosslinking occurs via an electrochemical reaction. Other crosslinking reactions are also of interest such as
dimerization, Diels-Alder reaction, Michael-type additions, metathesis, reactions with isocyanates, or any other condensation reactions.
Irradiation is the process by which an item is exposed to radiation. The radiation of the present invention is ultraviolet light (the ultraviolet region has a
wavelength between 1 and 400 nm) , preferably at wavelengths from 250 nm to 400 nm, more preferably 300 nm to 380nm, or visible light (400 nm to 850 nm) , or any other form of radiation including microwave irradiation.
According to the methods of the present invention, the initiator in step a) is a diol, a triol, a tetra-ol, a diamine, a triamine, tetra-amine, dithiol, trithiol or a tetra-thiol. A diol, triol or tetra-ol has two, three or four organic alcoholic functions -OH (also called hydroxy group), respectively. A diamine, triamine or tetra-amine has two, three or four amine groups -NH- or -NH2, respectively. A dithiol, trithiol or tetrathiol has two, three or four -SH groups, respectively.
According to a preferred embodiment of the present invention, the initiator in step a) is chosen from the group 1 , 2-ethanediol , 1 , 3-propanediol , 1 , 4-butanediol , 1,5- pentanediol, 1 , 6-hexanediol , 1 , 8-octanediol . They
respectively have the chemical formula: C2H4(OH)2, C3H6(OH)2, C4H8(OH)2, C5H10(OH)2, C6H12(OH)2, C8H16(OH)2.
According to the methods of the present invention, the initiator concentration in step a) is in the range 10~6 to 10~2 mol per total mol of monomers.
According to a preferred embodiment of the present invention, step a) comprises a catalyst.
A catalyst is a chemical substance able to
accelerate or enhance a reaction without being consumed in the chemical reaction.
According to another preferred embodiment of the present invention, step a) comprises a catalyst which is a stannous alkyloate or a metallic compound chosen from the group stannous octoate or metallic zinc. Other catalysts are also of interest, such as tin, titanium, iron, zink or calcium based catalysts. According to the methods of the present invention, the catalyst concentration in step a) is in the range of 10_i to lCT2 mol per total mol of the monomers.
According to the methods of the present invention, the polyol oligomers in step a) are prepared from two monomers chosen from the group cyclic carbonates and/or a cyclic esters and/or linear carbonates and/or linear esters and/or hydroxycarboxylic acids with a ratio between monomers which is in the range of 0.01:100 to 100:0.01 by mol.
According to the methods of the present invention, the preparation of the polyol oligomers in step a)
comprises: i) mixing the cyclic carbonates and/or cyclic esters and/or linear carbonates and/or linear esters and/or hydroxycarboxylic acids with the initiator at a temperature in the range of 20°C to 200°C, under inert atmosphere and under stirring; ii) adding the catalyst; iii) maintaining the temperature of step a) under stirring for 15 minutes to 4 days; iv) bringing the mixture at room temperature.
The temperature range in step i) is between 20°C to 200°C, such as 20 °C, 25°C, 30°C, 35°C, 40°C, 45°C, 50°C, 55°C, 60°C, 65°C, 70°C, 75°C, 80°C, 85°C, 90°C, 95°C, 100°C, 105°C, 110°C, 115°C 120°C, 125°C, 130°C, 135°C, 140°C, 145°C, 150°C, 155°C, 160°C, 165°C, 170°C, 175°C, 180°C, 185°C, 190°C, 195°C, 200°C. The polymerization reaction in step a) can also be done at different temperature than the temperature at which the monomers are mixed.
Stirring in step iii) is mechanical stirring, ultrasonic mixing or magnetic agitation. Stirring until homogeneity is stirring until forming a uniform mixture to the naked eye.
According to the methods of the present invention, the functionalization is carried out with methacrylic anhydride or methacryloyl chloride and an amine in an organic solvent. The solvent can be a chlorinated organic solvent .
A methacrylate group is an organic functional group with the formula CH2=C (CH3) COO . An anhydride is the group 0=C-0-C=0. Methacrylic anhydride has the formula C8H10O3.
Methacryloyl chloride has the formula C4H5C10.
An amine is a chemical group comprising a -N-, the amine can be primary, -NH2, secondary -RNH, or tertiary -RNR.
A chlorinated organic solvent is a hydrocarbon containing chlorine. Examples are dichloromethane,
chloroform, carbontetrachloride . Dichloromethane has the formula CH2C12, chloroform CHC13, carbontetrachloride CC14.
According to the methods of the present invention, the crosslinking in step c) is photocrosslinking with ultraviolet light or visible light. The ultraviolet light used in the present invention has a wavelength between 1 and 400 nm, preferably at wavelengths from 250 nm to 400 nm, more preferably 300 nm to 380nm. The visible light has a wavelength between 400 nm and 850 nm.
The initiator of the crosslinking can be a
photoinitiator, a chemical substance that produces
radical/ionic species upon exposure to light, or a thermal initiator, a chemical substance that produces the
radical / ionic species with sensitivity to temperature, or a redox initiator, a chemical substance that produces the radical / ionic species by a redox reaction.
According to the methods of the present invention, the initiator is chosen from the class hydroxyketone, aminoketone, phenylglyoxylate .
A hydroxyketone has the organic groups alcohol -OH and ketone C=0. An aminoketone has an amine NH2 and a ketone C=0. A phenylglyoxylate has an aromatic C6H6 hydrocarbon and an ester function -COO- . According to the methods of the present invention, the photoinitiator is 2-hydroxy-4 ' - (2-hydroxyethoxy ) -2- methylpropiophenone . 2-hydroxy-4 ' - (2-hydroxyethoxy) -2- methylpropiophenone has the formula C12H16O4.
According to the methods of the present invention, the methods further comprise a step b2) between step b) and step c) of compression molding or extrusion or injection molding or casting of the functionalized oligomer obtained in step b) . The process of casting can also be solvent casting.
Compression molding is a method of molding in which the molding material, generally preheated, is first placed in an open, (heated) mold cavity. The mold is closed with a top force or plug member, pressure is applied to force the material into contact with all mold areas, while heat and pressure are maintained until the molding material has cured. Extrusion is a process used to create objects of a fixed cross-sectional profile. A material is pushed or drawn through a die of the desired cross-section. The main
advantages of this process over other manufacturing
processes are its ability to create very complex cross- sections. It also forms finished parts with an excellent surface finish. Injection molding is a manufacturing process for producing parts of polymer materials. The material is fed into a heated barrel, mixed, and forced into a mold cavity where it cools and hardens to the configuration of the mold cavity. After a product is designed, molds are made in usually either steel or aluminium, and precision-machined to form the features of the desired part. Injection molding is widely used for manufacturing a variety of parts. Casting is a process for forming thermoplastic articles by dipping a male mold into a liquid polymer, a solution or dispersion of the polymer and drawing off the solvent (if any present) to leave a layer of plastic film adhering to the mold.
According to the methods of the present invention, the shape changes in the polymer network material are induced by stimuli chosen from the group thermal, electric, pH, magnetic stimuli, light or gamma irradiation, or water content modification.
According to another aspect of the present
invention, amorphous polymeric materials with shape memory are obtainable by the methods of the present invention.
According to another aspect, the amorphous polymeric network materials with shape memory of the present invention are used for coating surfaces, as a protective layer for thermal insulation and/or for anti-oxidation insulation, for the manufacture of packaging materials.
According to yet another aspect, the amorphous polymeric network materials with shape memory of the present invention are used for preparing medical devices.
According to still another aspect of the present invention, the amorphous polymeric network materials with shape memory are used to prepare implants, used in tissue engineering, or drug delivery.
The present invention is further described by the figure and Examples herewith. The Examples illustrate the present invention and do not aim to limit its scope. Figure 1: Photo-crosslinked network prepared from a
DLLA (0.6) : TMC (0.4) oligomers with number average molecular weight of approximately 30000g/mol displaying shape memory behaviour. A) Shape at 10 °C (Temporary shape); B) Shape at 37 °C after 20 s;
C) Shape at 37 °C after 100 s (Permanent shape) .
Examples Example 1
A series of poly (D, L-lactide-co-trimethylene carbonate) diols and their corresponding homopolymers , i.e oligomeric Poly (DLLA) and Poly (TMC) diols, are synthesized by ring opening polymerization of DLLA and TMC using 1 , 6-hexanediol as an initiator and SnOct2 as a catalyst. The composition in the oligomeric copolymers (TMC:DLLA molar ratio) is varied from 0 to 1 in steps of 0.2. The employed monomer feed compositions and the amounts of initiator are shown in Table 1. In a typical reaction, the required amounts of TMC or DLLA or their mixtures along with the initiator, are charged into a 250 mL three necked round bottom flask equipped with magnetic stirrer under the flow of nitrogen (Table 1) . The number average molecular weight of oligomers can be varied by adjusting the monomer to initiator molar ratio. The flask is then evacuated and purged with nitrogen several times and heated to 130 °C under a nitrogen atmosphere. To the
homogenous mixture, SnOct2 (2xl0~4 mole of monomers) is charged immediately and the reaction is continued for 3 days at this temperature. Upon completion of the reaction, the mixture is cooled to room temperature, dissolved in
chloroform and precipitated in an excess of cold ethanol to remove unreacted TMC or DLLA. The purified oligomers are then dried in a vacuum oven for 2 days at 30 °C.
Hydroxyl-group terminated (co) polymeric oligomers, with targeted number average molecular weights of 30000 g/mol (designated by 30k) were prepared. Copolymeric oligomers containing 40 mol% TMC oligomers with number average molecular weights of 5000 and 10000 g/mol (designated by 5k and 10k, respectively) were also prepared.
Table 1. Polymerization recipes for preparing oligomeric PDLLA and PTMC diols and copolymer oligomeric diols with intended number average molecular weights of 5000, 10000, and 30000 g/mol.
Figure imgf000019_0001
The synthesized diols are subsequently methacrylate- endcapped. This reaction is carried out using methacrylic anhydride in the presence of triethyl amine (TEA) . The oligomers, methacrylic anhydride, and TEA are measured out in 1:4:4 molar ratios. The pre-dried oligomer is charged into a 3-necked round bottom flask under nitrogen atmosphere containing 100 mL of anhydrous dichloromethane. After dissolution of the oligomer, methacrylic anhydride and TEA are charged into the flask. The reaction is allowed to proceed for 7 days at room temperature. Purification is done by precipitation in cold excess ethanol. The precipitated macromer is then vacuum dried for 2 days at room temperature and stored at -20 °C. Example 2
The photo-crosslinking process is carried out by UV light (365 nm) using Irgacure 2959 as a photoinitiator . The photoinitiator (2 wt% of the macromer) is dissolved in chloroform (1 mL/g macromer) and then added to the macromer. The mixture is then gently heated and stirred manually until a homogenous mixture is obtained. The solvent is
subsequently evaporated by applying vacuum. The mixture is then slightly heated to soften it and shaped by compression molding. The sheets were then heated to 75 °C and irradiated for 500 sec under a blanket of nitrogen gas. The distance between the film and UV lamps was 10 cm. The films were carefully taken out from the mould and punched into the desired shapes. Several of the specimens were extracted using chloroform. In these cases, network strips were immersed in an excess of chloroform for 24 h. During the extraction, the strips were regularly moved with tweezers. After 24 h, the extraction media was replaced with fresh chloroform and small amounts of methanol were gradually added to the chloroform to shrink the specimens without forming cracks or defects. The shrunk samples were then transferred to a vacuum oven and dried for 36 hours at 40 °C and 0.2 Bar . Example 3
Macromers based on PTMC and PDLLA homopolymeric oligomers and the TMC and DLLA copolymeric oligomers, which form covalently crosslinked networks upon photo-initiated radical polymerization, were prepared by the two step process of example 1.
These macromers, formed by functionalization of the
hydroxyl-group terminated TMC and DLLA oligomers using methacrylic anhydride, were then crosslinked according to the photo-polymerization process of example 2. Table 2 gives an overview of the properties of the macromers and the obtained networks. Tables 3 to 6 give an overview of the thermal and mechanical properties of the prepared networks.
Table 2. Characterization of the synthesized macromers and their corresponding networks
Figure imgf000021_0001
a) molar ratios of the monomers in the feed b) measurements of the degree of swelling and the sol fraction were conducted using chloroform. Values between parentheses are standard deviations .
c) n.d. = not determined
Table 3. Glass transition temperatures of the different networks as determined by DSC and DMA
Figure imgf000022_0001
a) Tg determined by differential scanning calorimetry (DSC) on non- extracted specimens
b) Tg determined by DSC on specimens extracted using dichloromethane c) Tg determined by dynamic mechanical analysis (temperature at which tan5 reaches a maximum value) .
d) n.d. = not determined Differential scanning experiments were conducted at a heating rate of 10 °C per minute using a PerkinElmer Pyris DSC. Temperature scans were done between -40 °C and 90 °C, after first heating the specimens to 90 °C and then cooling to -40 °C at 5 °C per minute.
Table 4. Dynamic mechanical analysis of the prepared photocrosslinked networks showing the rigidity of the materials at the different temperatures.
Figure imgf000023_0001
Dynamic mechanical analysis (DMA)
The dynamic mechanical thermal properties of extracted photocrosslinked networks measuring 5x20x0.5 mm3, were determined using a PerkinElmer DMA 7 operating in the tensile mode. Storage modulus and loss tangent values (tan δ) were determined between -50 °C and 100 °C at a heating rate of 2 °C/min. The static strain was 200 mN. Table 5. Mechanical properties of photo-crosslinked networks prepared from macromers with number average molecular weights of approximately 30000 g/mol after extraction and drying determined by tensile testing at room temperature (23 °C) . Values between parentheses are standard deviations.
Figure imgf000024_0001
a) Tensile test conducted at 20 °C
Tensile testing Testing was done according to ASTM D882-91, narrow strips
(5x100x0.5 mm3) were tested using a Zwick Z020 tensile tester at room temperature. The crosshead speed was 50 mm/min and the grip to grip separation was 50 mm. The Young's modulus values, elongations at break and the maximal tensile
strengths of the photocrosslinked specimens were determined from the stress-strain curves. Table 6. Mechanical properties of photo-crosslinked networks prepared from macromers with different number average molecular weights of approximately 30000, 10000 and 5000 g/mol after extraction and drying determined by tensile testing at room temperature (23 °C) and by dynamic mechanical analysis. Values between parentheses are standard
deviations .
Figure imgf000025_0001
The mechanical properties of the polymer network materials of the present invention are significantly better than the materials known up to date: the tensile strength and
elongation at break values are equal or higher in the polymer network material of the present invention.
Example 4
The amorphous photo-crosslinked networks display shape memory properties. For instance, when a network is prepared from a DLLA ( 0.6 ) : TMC ( 0.4 ) macromer with number average molecular weight of approximately 30000g/mol in the shape of a spring by photo-crosslinking using a transparent mould, the specimen can be deformed to a linear shape by heating the spring to above the glass transition temperature, stretching the specimen and cooling the stretched specimen to below the glass transition temperature. This specimen maintains is temporary shape while it is kept at
temperatures below the glass transition temperatures. As Figure 1 shows, heating the specimen to 37 °C, which is 11 °C above the glass transition temperature measured by DSC, rapidly returns the specimen to its original (spring) shape.
The shape memory polymers investigated here present an amorphous crosslinked network. Amorphous resorbable networks with glass transition temperatures below or close to body temperature are most suited for biomedical applications where the loading conditions are relatively low.

Claims

1. Method for preparing amorphous resorbable polymeric network materials with shape memory comprising:
a) the preparation of polyol oligomers with a number average molecular weight higher than or equal to 900 g/mol per hydroxy-group, by polymerization of cyclic carbonates and/or a cyclic esters and/or linear carbonates and/or linear esters and/or
hydroxycarboxylic acids using an initiator; b) the functionalization of the polyol
oligomers with at least two unsaturated group;
c) the crosslinking of the functionalized
oligomers obtained in step b) using an initiator;
2. Method for preparing amorphous resorbable polymeric network materials with shape memory according to claim 1, wherein the number average molecular weight per hydroxy-group of the polyol oligomers of step a) is in the range 1000 to 150000 g/mol, preferably 1500 to 100000 g/mol, more preferably 2000 g/mol to 50000 g/mol, most preferably 5000 g/mol to 30000 g/mol.
3. Method for preparing amorphous resorbable polymeric network materials with shape memory according to claim 1 or 2, wherein the functionalization in step b) is end-functionalization or co-polymerization.
4. Method for preparing amorphous resorbable polymeric network materials with shape memory according to any one of claims 1 to 3, wherein the polyol oligomers are chosen from the group diols, triols, tetra-ols or penta-ols.
5. Method for preparing amorphous resorbable polymeric network materials with shape memory according to any one of claims 1 to 4, wherein the cyclic carbonates are chosen from the group trimethylene carbonate, ethylene carbonate, propylene carbonate, diethylene glycol-bis- allyl-carbonate and derivatives thereof.
6. Method for preparing amorphous resorbable polymeric network materials with shape memory according to any one of claims 1 to 5, wherein the linear carbonates are chosen from the group diethyl carbonate, diphenyl carbonate and derivatives thereof.
7. Method for preparing amorphous resorbable polymeric network materials with shape memory according to any one of claims 1 to 6, wherein cyclic esters are chosen from the group lactones, D,L-lactide, glycolide and
derivatives thereof.
8. Method for preparing amorphous resorbable polymeric network materials with shape memory according to any one of claims 1 to 7, wherein the linear esters are chosen from the group fumaric acid monoethyl ester, fumaric acid diethyl ester, dimethylterephtalate, diethyl
terephtalate and derivatives thereof.
9. Method for preparing amorphous resorbable polymeric network materials with shape memory according to any one of claims 1 to 8, wherein the polymerization in step a) is a ring-opening polymerization or polycondensation .
10. Method for preparing amorphous resorbable polymeric network materials with shape memory according to any one of claims 1 to 9, wherein the unsaturated end group is chosen from the group acrylate, methacrylate, fumarate, diacrylate, triacrylate.
11. Method for preparing amorphous resorbable polymeric network materials with shape memory according to any one of claims 1 to 10, wherein the crosslinking in step c) is photocrosslinking, thermal crosslinking or redox crosslinking .
12. Method for preparing amorphous resorbable polymeric network materials with shape memory according to any one of claims 1 to 11, wherein the initiator in step a) is a diol, a triol, a tetra-ol, an diamine, a triamine, a tetra-amine, a dithiol, a trithiol, or a tetra-thiol.
13. Method for preparing amorphous resorbable polymeric network materials with shape memory according to any one of claims 1 to 12, wherein the initiator in step a) is chosen from the group 1 , 2-ethanediol , 1 , 3-propanediol ,
1 , 4-butanediol , 1 , 5-pentanediol , 1 , 6-hexanediol , 1,8- octanediol .
14. Method for preparing amorphous resorbable polymeric network materials with shape memory according to any one of claims 1 to 13, wherein the initiator in step a) is in the range 10~6 to 10~2 mol per total mol of monomers.
15. Method for preparing amorphous resorbable polymeric network materials with shape memory according to any one of claims 1 to 14, wherein step a) comprises a catalyst .
16. Method for preparing amorphous resorbable polymeric network materials with shape memory according to any one of claims 1 to 15, wherein step a) comprises a catalyst which is a stannous alkyloate or a metallic
compound chosen from the group stannous octoate or metallic zinc .
17. Method for preparing amorphous resorbable polymeric network materials with shape memory according to claim 15 or 16, wherein the catalyst concentration in step a) is in the range of 10~8 to 10~2 mol per total mol of the monomers .
18. Method for preparing amorphous resorbable polymeric network materials with shape memory according to any one of claims 1 to 17, wherein the polyol oligomers in step a) are prepared from two monomers chosen from the group cyclic carbonates and/or a cyclic esters and/or linear carbonates and/or linear esters and/or hydroxycarboxylic acids with a ratio between monomers which is in the range of 0.01:100 to 100:0.01 by mol.
19. Method for preparing amorphous resorbable polymeric network materials with shape memory according to any one of claims 1 to 18, wherein the preparation of the polyol oligomers in step a) comprises:
i) mixing the cyclic carbonates and/or cyclic esters and/or linear carbonates and/or linear esters and/or hydroxycarboxylic acids with the initiator at a temperature in the range of 20°C to 200°C, under inert atmosphere and under stirring;
ii) optionally adding a catalyst;
iii ) maintaining the temperature of step a)
under stirring for 15 minutes to 4 days; iv) bringing the mixture at room temperature.
20. Method for preparing amorphous resorbable polymeric network materials with shape memory according to any one of claims 1 to 19, wherein the functionalization is carried out with methacrylic anhydride or methacryloyl chloride and an amine in an organic solvent
21. Method for preparing amorphous resorbable polymeric network materials with shape memory according to any one of claims 1 to 20, wherein the crosslinking in step c) is a photocrosslinking obtained with ultraviolet light or visible light.
22. Method for preparing amorphous resorbable polymeric network materials with shape memory according to any one of claims 1 to 21, wherein the initiator in step c) is chosen from the class hydroxyketone, aminoketone, phenylglyoxylate .
23. Method for preparing amorphous resorbable polymeric network materials with shape memory according to any one of claims 1 to 22, wherein the initiator in step c) is 2-hydroxy-4 ' - (2-hydroxyethoxy) -2-methylpropiophenone .
24. Method for preparing amorphous resorbable polymeric network materials with shape memory according to any one of claims 1 to 23, wherein the method further comprises a step bl) between step b) and c) of compression molding or extrusion or injection molding or casting of the functionalized oligomer.
25. Method for preparing amorphous resorbable polymeric network materials with shape memory according to any one of claims 1 to 24, wherein shape changes are induced by stimuli chosen from the group thermal, electric, pH, magnetic stimuli, light or gamma irradiation, water content modification.
26. Amorphous resorbable polymeric network materials with shape memory obtainable by any one of claims 1 to 25.
27. Use of the amorphous resorbable polymeric network materials with shape memory as defined in claim 26 for coating surfaces, as a protective layer for thermal insulation and/or for anti-oxidation insulation, for the manufacture of packaging materials.
28. Use of the amorphous resorbable polymeric network materials with shape memory as defined in claim 26 for preparing medical devices.
29. Use of the amorphous resorbable polymeric network materials with shape memory as defined in claim 26 for preparing implants, used in tissue engineering, or drug delivery .
PCT/EP2010/057075 2010-05-21 2010-05-21 Amorphous resorbable polymeric network materials with shape memory WO2011144257A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/EP2010/057075 WO2011144257A1 (en) 2010-05-21 2010-05-21 Amorphous resorbable polymeric network materials with shape memory

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/EP2010/057075 WO2011144257A1 (en) 2010-05-21 2010-05-21 Amorphous resorbable polymeric network materials with shape memory

Publications (1)

Publication Number Publication Date
WO2011144257A1 true WO2011144257A1 (en) 2011-11-24

Family

ID=43414777

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2010/057075 WO2011144257A1 (en) 2010-05-21 2010-05-21 Amorphous resorbable polymeric network materials with shape memory

Country Status (1)

Country Link
WO (1) WO2011144257A1 (en)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030191276A1 (en) * 2002-02-26 2003-10-09 Mnemoscience Gmbh Polymeric networks
WO2003088818A2 (en) * 2002-04-18 2003-10-30 Mnemoscience Gmbh Biodegradable shape memory polymeric sutures
WO2004006885A2 (en) * 2002-07-10 2004-01-22 Mnemoscience Gmbh Systems for releasing active ingredients, based on biodegradable or biocompatible polymers with a shape memory effect
EP1801140A1 (en) * 2005-12-22 2007-06-27 Mnemoscience GmbH Macro-diacrylate
EP2075279A1 (en) * 2007-12-28 2009-07-01 Mnemoscience GmbH Production of shape memory polymer articles by molding processes

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030191276A1 (en) * 2002-02-26 2003-10-09 Mnemoscience Gmbh Polymeric networks
WO2003088818A2 (en) * 2002-04-18 2003-10-30 Mnemoscience Gmbh Biodegradable shape memory polymeric sutures
US20040015187A1 (en) * 2002-04-18 2004-01-22 Mnemoscience Corporation Biodegradable shape memory polymeric sutures
WO2004006885A2 (en) * 2002-07-10 2004-01-22 Mnemoscience Gmbh Systems for releasing active ingredients, based on biodegradable or biocompatible polymers with a shape memory effect
EP1801140A1 (en) * 2005-12-22 2007-06-27 Mnemoscience GmbH Macro-diacrylate
EP2075279A1 (en) * 2007-12-28 2009-07-01 Mnemoscience GmbH Production of shape memory polymer articles by molding processes

Similar Documents

Publication Publication Date Title
Bruggeman et al. Biodegradable xylitol‐based polymers
US6984393B2 (en) Biodegradable elastomer and method of preparing same
US7217744B2 (en) Polymeric networks
CN103709691B (en) Biodegradable crosslinking polymer and preparation method thereof
EP2825581B1 (en) Segmented, semicrystalline poly(lactide-co-epsilon-caprolactone) absorbable copolymers
EP2576646B1 (en) Method for preparing a degradable polymer network
Kuhnt et al. Poly (caprolactone-co-trimethylenecarbonate) urethane acrylate resins for digital light processing of bioresorbable tissue engineering implants
CN105694008B (en) A kind of preparation method of the adjustable macromolecule polyester composite of biodegradation rate
US8129478B2 (en) Polyester anhydride-based biopolymers
JP2017505832A (en) Absorbent polymer blend compositions based on copolymers prepared from monofunctional and bifunctional polymerization initiators, processing methods, and medical devices thereby
EP2842583A1 (en) Biodegradable and bioerodible polyurethanes, method of preparation thereof and use thereof
Li et al. Preparation of mechanically-tough and thermo-responsive polyurethane-poly (ethylene glycol) hydrogels
Wright et al. Synthesis of functionalized poly (lactic acid) using 2-bromo-3-hydroxypropionic acid
Hou et al. Creep-resistant elastomeric networks prepared by photocrosslinking fumaric acid monoethyl ester-functionalized poly (trimethylene carbonate) oligomers
Wang et al. Synthesis of a photocurable acrylated poly (ethylene glycol)-co-poly (xylitol sebacate) copolymers hydrogel 3D printing ink for tissue engineering
Xue et al. PEGylated poly (ester amide) elastomers with tunable physico-chemical, mechanical and degradation properties
Feng et al. Synthesis and characterization of hydrophilic polyester‐PEO networks with shape‐memory properties
EP1641471B1 (en) Biocompatible polymer networks
Akdemir et al. Preparation of biocompatible, UV-cured fumarated poly (ether-ester)-based tissue-engineering hydrogels
Lei et al. Synthesis, characterization and cytocompatibility of a degradable polymer using ferric catalyst for esophageal tissue engineering
Theiler et al. Synthesis, characterization and in vitro degradation of 3D-microstructured poly (ε-caprolactone) resins
WO2011144257A1 (en) Amorphous resorbable polymeric network materials with shape memory
EP3908619B1 (en) Branched-block copolymer photo-crosslinker functionalized with photoreactive groups and its use for shaping degradable photo-crosslinked elastomers suitable for medical and tissue-engineering applications
EP3097134B1 (en) Biodegradable hydrogel with controlled lifetime and method of preparation thereof
Gréant et al. Digital light processing of poly (ε-caprolactone)-based resins into porous shape memory scaffolds

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 10726016

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 10726016

Country of ref document: EP

Kind code of ref document: A1