WO2001073420A1

WO2001073420A1 - Biosensor measuring device

Info

Publication number: WO2001073420A1
Application number: PCT/JP2001/002733
Authority: WO
Inventors: Yoshinobu Tokuno
Original assignee: Matsushita Electric Industrial Co., Ltd.
Priority date: 2000-03-30
Filing date: 2001-03-30
Publication date: 2001-10-04
Also published as: JP2001281197A

Abstract

A biosensor measuring device comprising sensor chips for changing the forming positions of electrode terminals according to differing reagents to be carried and differing production-lot-dependent calibration curve correction formulae, and a measuring instrument for forming connection terminals corresponding to the electrode terminal forming positions of a plurality of types of sensor chips, the measuring instrument discriminating the types of sensor chips by changing over switches one after another and detecting which of the connection terminals conducts. The biosensor measuring device having the above construction can electrochemically measure components in liquid sample such as blood by attaching disposable sensor chips to the measuring instrument, and automatically change over the operation of the measuring instrument according to differing reagents carried on sensor chips and differing calibration curve corrections for correcting obtained output values.

Description

Description Biosensor measurement device

Technical field

The present invention relates to a biosensor measuring device, and more particularly to a biosensor measuring device that takes a liquid sample into a sensor chip, connects the sample to a measuring device, and electrochemically measures the concentration of a specific component. Background art

As a conventional biosensor measurement device, a very small amount of body fluid such as blood is collected from the human body into a disposable sensor chip, and the current value generated by the reaction with a specific reagent carried on the sensor chip is measured by the sensor. Some read with a measuring device to which the chip is connected, and measure the values of blood sugar, cholesterol, lactic acid, etc.

Such a disposable sensor chip is composed of a casing for collecting body fluid, an electrode, and a reagent, but portions other than the reagent carried on the sensor chip can be shared. Also in the measuring instrument, the hardware part can be used in essence by changing the calculation formula for converting the detected current value to the value corresponding to each concentration. However, in the case of common use, if a sensor chip for measuring blood glucose is connected to a measuring device for measuring cholesterol, for example, there is a problem that a normal measuring operation cannot be performed. By changing the shape of the connection part of the measuring instrument, incorrect connection between different types of sensor chips and the measuring instrument was prevented.

Also, in the manufacturing process of forming reagents on the sensor chip, variations in performance for each lot cannot be avoided, so the measuring instrument corrects this manufacturing variation for the actually detected current value. It is necessary to display blood sugar and cholesterol levels.

For this reason, in the conventional biosensor measuring device, a calibration curve correction formula for correcting the variation of the output value of the sensor chip is set in the measuring device, and the sensor chip is provided with a calibration curve correction formula. Has attached a calibration curve correction type switching chip for each lot. Prior to the measurement, the user set the switching chip in the measuring instrument, set the calibration curve correction formula suitable for the sensor chip, and performed the measurement.

When the parts other than the reagents are shared among those shown in the above conventional configuration, the outer shape of the sensor chip and the shape of the connection part of the measuring device are changed according to each measurement purpose, so Since the production equipment, including the mold, had to be changed, the cost was high, and the common use had not been achieved.

In addition, the calibration curve correction must be set by the user using the switching tip, which makes it difficult and troublesome for visually impaired people, the elderly, and children. There was a problem that the correct value was not displayed when the line switching setting was forgotten.

The present invention has been made to solve such a problem, and the operation of the measuring instrument is automatically switched according to the difference between the reagent carried on the sensor chip and the calibration curve correction type, and the operation according to each measurement purpose is performed. It is an object of the present invention to provide a biosensor measurement device that can reduce the time and effort required for a setting operation and improve user operability. Disclosure of the invention

A biosensor measuring device according to the present invention (Claim 1) comprises: a sensor chip having a pair of electrode terminals for detecting an electric signal generated by a reaction between a liquid sample and a reagent; and a sensor chip attached to the sensor chip. A biosensor measuring device for electrochemically measuring a component in the liquid sample using a measuring instrument having a connection terminal electrically connected to an electrode terminal of the sensor chip. The pair of electrode terminals are formed at positions different from each other according to the type of the sensor chip, and the connection terminals of the measuring device are formed at positions corresponding to the respective electrode terminals of the plurality of types of sensor chips. The measuring device detects which connection terminal of the connection terminals is electrically connected to the electrode terminal of the sensor chip, and determines the type of the sensor chip. It is characterized by the following.

According to the biosensor measuring device having such a configuration, the operation of the measuring device can be automatically switched according to the position of the electrode terminal of the sensor chip. The operability of the user can be improved by reducing the time and effort required for the setting operation according to the purpose.In addition, regardless of the type of the sensor chip, members such as sensor chip casing can be shared, and the external shape of the sensor chip Since they can be shared, production costs can be reduced.

The biosensor measuring device according to the present invention (Claim 2) is the biosensor measuring device according to Claim 1, wherein a switch is connected to each of the plurality of connection terminals, According to another aspect of the present invention, the switch is sequentially switched to detect which of the plurality of connection terminals is electrically connected to the electrode terminal of the sensor chip.

According to the biosensor measuring device having such a configuration, the type of a plurality of sensor chips can be automatically determined by one measuring device by sequentially switching the switches.

The biosensor measuring device according to the present invention (Claim 3) is the biosensor measuring device according to Claim 2, wherein the measuring device is N (N is an arbitrary integer of 2 or more). It has a pair of connection terminals and can distinguish up to 2 ^N types of sensor chips.

According to the biosensor measuring device having such a configuration, it is possible to determine the types of the sensor chips of up to ^2N types with one measuring device having N pairs of connection terminals.

A biosensor measuring device according to the present invention (claim 4) comprises: a sensor chip having a pair of electrode terminals for detecting an electric signal generated by a reaction between a liquid sample and a reagent; and a sensor chip attached to the sensor chip. A biosensor measuring device for electrochemically measuring a component in the liquid sample using a measuring instrument having a connection terminal electrically connected to an electrode terminal of the sensor chip. Of the pair of electrode terminals, the first electrode terminal is formed at the same position regardless of the type of the sensor chip, and the second electrode terminal is formed at a different position depending on the type of the sensor chip. The measuring device includes: a connection terminal corresponding to the first electrode terminal; and a plurality of connection terminals corresponding to the second electrode terminal according to a type of the sensor chip. Serial any connection terminals of the plurality of connecting terminals to detect whether conduction with the electrode terminal of the sensor chip, determines the type of the sensor chip, and wherein the Is what you do.

According to the biosensor measuring device having such a configuration, the operation of the measuring device can be automatically switched in accordance with the position of the electrode terminal of the sensor chip, and as a result, the labor involved in the setting operation corresponding to each measurement purpose can be reduced. This reduces the operability of the user by reducing the size of the sensor chip.In addition, regardless of the type of the sensor chip, the sensor chip casing and other members can be used in common, and the external shape of the sensor chip can be shared, reducing production costs. Further, by fixing the position of one of the pair of electrode terminals of the sensor chip irrespective of the type of the sensor chip, a measuring device that can determine the type of the sensor chip with a simpler configuration can be realized.

The biosensor measuring device according to the present invention (claim 5) is the biosensor measuring device according to claim 4, wherein a switch is provided at each of the plurality of connection terminals corresponding to the second electrode terminal. Wherein the measuring device detects which of the plurality of connection terminals is conductive with the electrode terminal of the sensor chip by sequentially switching the switches. is there.

The biosensor measuring device according to the present invention (claim 6) is the biosensor measuring device according to claim 5, wherein the measuring device is an M (M is an arbitrary integer) type of sensor chip. When the type is determined, M connection terminals corresponding to the second electrode terminals of each of the sensor chips are provided.

According to the biosensor measuring device having such a configuration, the number of sensor terminals is equal to the number of connection terminals corresponding to the electrode terminals formed at different positions according to the type of the sensor chip, out of the pair of electrode terminals of the sensor chip. The type of the chip can be determined.

The biosensor measuring device according to the present invention (Claim 7) is the biosensor measuring device according to any one of Claims 1 to 6, wherein the sensor chip is provided in a liquid sample. The type of reagent to be supported and the position of the electrode terminal are changed according to the component to be measured.

According to the biosensor measurement device having such a configuration, the biosensor measurement device Measurement according to the reagent becomes possible.

The biosensor measuring device according to the present invention (Claim 8) is the biosensor measuring device according to any one of Claims 1 to 6, wherein the electrode terminal of the sensor chip comprises: It is formed at a different position according to the output characteristics of the sensor chip.

According to the biosensor measuring device having such a configuration, the measured value obtained from the measuring device can be corrected according to the output characteristics of the sensor chip. BRIEF DESCRIPTION OF THE FIGURES

FIG. 1 is a schematic configuration diagram showing a biosensor measurement device according to Embodiment 1 of the present invention.

FIG. 2 is a plan view of a sensor chip used for the biosensor measurement device according to Embodiment 1 of the present invention.

FIG. 3 is a schematic configuration diagram showing a biosensor measurement device according to Embodiment 2 of the present invention.

FIG. 4 is a plan view of a sensor chip used for a biosensor measurement device according to Embodiment 2 of the present invention. BEST MODE FOR CARRYING OUT THE INVENTION

Hereinafter, embodiments of the present invention will be described with reference to the drawings. The embodiment described here is merely an example, and the present invention is not necessarily limited to this embodiment.

Embodiment 1

Hereinafter, the biosensor measurement device according to the first embodiment of the present invention will be described with reference to FIGS. 1 and 2.

Embodiment 1 corresponds to Claims 1, 2, 3, 7, and 8. Claim 1 corresponds to Claim 1, Claim 2, Claim 3, Claim 7, and Claim 8.

FIG. 1 is a schematic configuration diagram showing a biosensor measurement device according to the first embodiment. In FIG. 1, a biosensor measuring device has a sensor chip 1 having a pair of electrode terminals 5 and 6 for detecting an electric signal generated by a reaction between a liquid sample and a reagent, and is mounted on the sensor chip 1. And a measuring instrument 2 provided with connection terminals 8, 9, 10 and 11 for making an electrical connection with the electrode terminals 5 and 6 of the sensor chip 1. Here, in the measuring device 2, only the configuration of the connection portion with the sensor chip 1 is schematically enlarged.

The sensor chip 1 is made by pressing a sheet of polyethylene resin or the like by press punching, etc.On a substrate 3, an enzyme reagent 4 that reacts with a liquid sample added thereto, and a reaction obtained between the working electrode and the counter electrode by an enzyme reaction It has electrode terminals 5 and 6 for detecting the current with the measuring instrument 2.

The connection part of the measuring instrument 2 has four connection terminals 8, 9, 10, 11 formed on a connector 7 molded of resin so as to engage with the outer shape of the sensor chip 1. The pair of electrode terminals 5 and 6 of the sensor chip 1 are formed at different positions depending on the type of the sensor chip. That is, one of the connection terminals 8 and 9 is connected to the electrode terminal 5 of the sensor chip 1, and one of the connection terminals 10 and 11 is connected to the electrode terminal 6. Switches 12, 9, 13, 14, and 15 are connected to connection terminals 8, 9, 10, and 11, respectively, and connected to the electric circuit (not shown) inside measuring instrument 2. Signal lines 16 and 17 and connection terminals 8, 9, 10 and 11 can be selectively connected. Here, the combinations that connect switches 12, 13, 14, and 15 are: switch 12 and switch 15, switch 12 and switch 14, switch 13 and switch 15, switch 13 and switch 14, and so on. The switching is performed sequentially so as to close the switch in any combination.

As shown in FIG. 2, for example, the sensor chip 1 has exactly the same outer shape, but electrode terminals 5 and 6 are formed at different positions due to differences in reagents or manufacturing lots. There are four types: Figure 2 (b), Figure 2 (c), and Figure 2 (d). Here, for example, Fig. 2 (a) is a sensor chip for blood glucose, Fig. 2 (b) is a sensor chip for cholesterol, Fig. 2 (c) is a sensor chip for lactic acid, and Fig. 2 (d) is urine. It shall be used as a sensor chip for acid. Note that the calibration curve correction formulas for different production lots correspond to the electrode terminal positions and the calibration curve correction formula to be applied. You.

When such a sensor chip 1 is attached to the measuring device 2, the measuring device 2 immediately switches the switches 12, 13, 14, and 15 as follows, and conducts with the electrode terminals 5 and 6. Detect the connection terminal.

First, only the switches 13 and 14 are closed, and if a response is obtained, that is, if conduction is detected, the sensor chip 1 is determined to be a blood glucose sensor chip. If no response is obtained, only switches 13 and 15 are closed, and if a response is obtained, it is determined that sensor chip 1 is a cholesterol sensor chip. If no response is obtained, only switches 12 and 14 are closed, and if a response is obtained, sensor chip 1 is determined to be a lactic acid sensor chip. If a response is not obtained, the switches 12 and 15 are closed and the conduction is detected to determine that the sensor chip 1 is a uric acid sensor chip.

It is not necessary to perform all the switching operations corresponding to the four types of the sensor chip 1 for the operation of switching the switch of the measuring device 2, and the switching operation can be completed when the conductive electrode terminal can be detected. .

As described above, in the biosensor measurement device according to the first embodiment, the pair of electrode terminals 5 and 6 are formed at different positions on the sensor chip 1 according to differences in reagents, calibration curve correction, and the like. The connection terminals 8, 9, 10 and 11 are formed in the sensor chip 1 corresponding to the positions of the electrode terminals 5 and 6 of the sensor chip 1, so that the sensor chip 1 can handle the reagents Measurement ゃ Calibration curve correction can be automatically set for the measured values obtained.In addition, regardless of the type of the sensor chip 1, members such as the casing of the sensor chip 1 can be shared, The external shape of (1) can be shared, and as a result, costs can be reduced.

In the first embodiment, the case where two pairs of connection terminals are provided in the measuring device 2 has been described. However, it is possible to increase the number of the connecting terminals further, and the measuring device 2 has N pairs (N is an integer of 2 or more). When the connection terminals are provided, 2 ^N types of sensor chips can be distinguished. Embodiment 2

Hereinafter, a biosensor measurement device according to Embodiment 2 of the present invention will be described with reference to FIGS. 3 and 4. FIG. . The second embodiment corresponds to claims 4, 5, 5, 6, 7, and 8.

FIG. 3 is a schematic configuration diagram showing a biosensor measurement device according to the second embodiment.

In FIG. 3, a biosensor measuring device includes a sensor chip 21 having a pair of electrode terminals 25 and 26 for detecting an electric signal generated by a reaction between a liquid sample and a reagent; 1 and a measuring device 22 provided with connection terminals 28, 29, 30 and 31 for making an electrical connection with the electrode terminals 25 and 26 of the sensor chip 21. Here, in the measuring device 22, only the connection portion with the sensor chip 21 is schematically enlarged.

The sensor chip 21 is provided on a substrate 23 obtained by stamping a sheet of polyethylene resin or the like onto a substrate 23. The reagent 2.4 reacts with the added liquid sample and the reaction current obtained by the reaction with the reagent. The electrode terminals 25 and 26 for detection are formed respectively.

The mounting portion of the measuring instrument 22 has four connection terminals 28, 29, 30, and 31 formed on a connector 27 molded with resin so as to engage with the outer shape of the sensor chip 21. . Among the pair of electrode terminals 25 and 26 of the sensor chip 21, the electrode terminal 25 is formed at the same position regardless of the type of the sensor chip 21, and the electrode terminal 26 is formed of the sensor chip 21. It is formed at a different position according to the type. That is, the connection terminal 28 is connected to the electrode terminal 25 of the sensor chip 21, and any one of the connection terminals 29, 30, 31 is connected to the electrode terminal 26. ing. Switches 32, 33, and 34 are connected to connection terminals 29, 30, and 31, respectively, and are connected to the electric circuit (not shown) inside measuring instrument 22. The signal line 37 and the connection terminals 29, 30 and 31 can be selectively connected. The difference from the first embodiment is that one electrode terminal 25 of the pair of electrode terminals of the sensor chip is formed at the same position, and the connection terminal of the measuring device 22 also corresponds to the electrode terminal 25. The connection terminal is a common connection terminal 28, and the other three connection terminals 29, 30 and 31 are sequentially switched.

The sensor chip 21 has exactly the same outer shape as shown in FIG. 4, for example. However, there are three types, Fig. 4 (a), Fig. 4 (b), and Fig. 4 (c), depending on the difference in the calibration curve correction formula due to the difference in reagents or production lots. Here, for example, it is assumed that FIG. 4 (a) is used as a sensor chip for blood glucose, FIG. 4 (b) is used as a sensor chip for cholesterol, and FIG. 4 (c) is used as a sensor chip for lactic acid.

When such a sensor chip 21 is attached to the measuring device 22, the measuring device 22 sequentially switches the switches 32, 33, and 34 as follows. '

If a response is obtained when the switch 32 is connected, it is determined that the sensor chip 21 is a blood glucose sensor chip. If a response is obtained when the switch 33 is connected, it is determined that the sensor chip 21 is a cholesterol sensor chip. If a response is obtained when the switch 34 is connected, the sensor chip 21 is determined to be a lactic acid sensor chip.

As in the first embodiment, the switch switching operation can be completed when a connection terminal that is electrically connected to the electrode terminal of the sensor chip can be detected. In addition, if the measuring device 22 has M (M is an arbitrary integer) connection terminals corresponding to the electrode terminals 26 of each sensor chip 21, the type of the M types of sensor chips 21 can be determined. It is. As described above, in the biosensor measurement device according to the second embodiment, the electrode terminal 26 of the pair of electrode terminals of the sensor chip 21 is located at a different position according to a difference in a reagent, a calibration curve correction formula, or the like. The electrode terminals 25 are fixed at the same position regardless of the type of the sensor chip 21, and the connection corresponding to the positions of the electrode terminals 25, 26 of the sensor chip 21 in the measuring instrument 22 Since the terminals 28, 29, 30 and 31 are formed, the type of the sensor chip 21 is determined with a simpler configuration than the biosensor measurement device described in the first embodiment, and the sensor chip 2 is determined. It is possible to automatically set the calibration curve correction etc. for the measurement according to the reagent carried on 1 and the obtained measured value, and also to set the sensor chip 21 regardless of the type of sensor chip 21. Components, such as the sensor chip 21 and the external shape of the sensor chip 21 Rukoto can, as a result, it is possible to reduce the cost.

Further, the type of the sensor chip 21 corresponding to the number of connection terminals corresponding to the electrode terminals 26 of each sensor chip 21 provided in the measuring device 22 can be determined. Industrial applicability

The biosensor measuring device according to the present invention can be used as a biosensor measuring device capable of discriminating the type of a sensor chip and switching the operation of the measuring device according to the difference between the reagent and the calibration curve correction formula.

Claims

The scope of the claims

1. a sensor chip having a pair of electrode terminals for detecting an electric signal generated by a reaction between a liquid sample and a reagent; and a sensor chip mounted on the sensor chip and electrically connected to the electrode terminal of the sensor chip. In a biosensor measurement device for electrochemically measuring components in the liquid sample, using a measurement device having a connection terminal,

The pair of electrode terminals of the sensor chip are formed at different positions depending on the type of the sensor chip,

The connection terminal of the measuring device is formed at a position corresponding to each electrode terminal of the plurality of types of sensor chips,

The measuring device detects which connection terminal of the connection terminals is electrically connected to an electrode terminal of the sensor chip, and determines a type of the sensor chip.

A biosensor measuring device, characterized in that:

2. The biosensor measurement device according to claim 1,

Connecting a switch to each of the plurality of connection terminals;

The biosensor measuring device, wherein the measuring device detects which of the plurality of connection terminals is electrically connected to an electrode terminal of the sensor chip by sequentially switching the switches.

3. In the biosensor measuring device according to claim 2,

The measuring instrument has N (N is an arbitrary integer of 2 or more) pairs of connection terminals, and is capable of discriminating a maximum of 2 ^N types of sensor chips.

A biosensor measuring device, characterized in that:

4. A sensor chip provided with a pair of electrode terminals for detecting an electric signal generated by a reaction between the liquid sample and the reagent, and an electrical connection with the electrode terminal of the sensor chip mounted on the sensor chip. A biosensor measuring device for electrochemically measuring a component in the liquid sample using a measuring device having a connection terminal comprising: a first electrode terminal among a pair of electrode terminals of the sensor chip; Are formed at the same position regardless of the type of the sensor chip, the second electrode terminals are formed at different positions according to the type of the sensor chip, The measuring device includes: a connection terminal corresponding to the first electrode terminal; and a plurality of connection terminals corresponding to the second electrode terminal according to a type of the sensor chip. A biosensor measuring device, comprising: detecting which one of the connection terminals conducts with an electrode terminal of the sensor chip, and determining the type of the sensor chip.

5. The biosensor measuring device according to claim 4,

A switch is connected to each of the plurality of connection terminals corresponding to the second electrode terminal, and the measuring device determines which of the plurality of connection terminals is electrically connected to the electrode terminal of the sensor chip. Is detected by sequentially switching the switches.

6. The biosensor measurement device according to claim 5,

When determining the types of M (M is an arbitrary integer) types of sensor chips, the measuring device includes M connection terminals corresponding to the second electrode terminals of each of the sensor chips. Biosensor measuring device.

7. The biosensor measuring device according to any one of claims 1 to 6, wherein:

The sensor chip changes the type of the reagent to be supported and the position of the electrode terminal according to the component to be measured in the liquid sample.

A biosensor measuring device, characterized in that:

8. The biosensor measurement device according to any one of claims 1 to 6, wherein:

The electrode terminals of the sensor chip are formed at different positions according to the output characteristics of the sensor chip.

A biosensor measuring device, characterized in that: