WO2001058591A2 - Closed multi-well analytical plate with annular adhesive patches for analytical optical measurements - Google Patents
Closed multi-well analytical plate with annular adhesive patches for analytical optical measurements Download PDFInfo
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- WO2001058591A2 WO2001058591A2 PCT/EP2000/001003 EP0001003W WO0158591A2 WO 2001058591 A2 WO2001058591 A2 WO 2001058591A2 EP 0001003 W EP0001003 W EP 0001003W WO 0158591 A2 WO0158591 A2 WO 0158591A2
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- sealing material
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
- B01L3/50853—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates with covers or lids
Definitions
- the invention relates to a closed multiwell analysis plate for analytical optical measurements.
- multiwell technology has become widely used in recent years (e.g. Matthews PD, Wurtzel ET: high-throughput microplate format for producing and screening riboprobes from bacterial cells, Biotechniques. 1995 Jun, 18 (6), 1000-1002, 1004 or Wu P., Daniel-Issakani S., LaMarco K., Strulovici B .: an automated high throughput filtration assay: application to polymerase inhibitor identification ,; anal- Biochem.
- Gerasimova NS Steklova IV, Tuuminen T .: Fluorometric method for phenylalanine microplate assay adapted for phenylketonuria screening, Clin-Chem. 1989, Oct, 35 (10), 21 12-21 15 or Collins L, et al. Microplate alamar blue assay versus BACTEC 460 System for high-throughput screening of compounds against Mycobacterium tuberculosis and Mycobacterium avium. Antimicrob Agents Chemother. 1997 May; 41 (5): 1004-9. Herrero ME, et al. Fluorometric microassay to quantify microsomal epoxide hydrolase in 96-well plates. Anal Biochem.
- the radiation or the evaluated optical signals are completely or partially suppressed by the sealing material; at least there is inhomogeneous signal interference via the large number of individual collecting vessel openings in the multiwell analysis plate.
- the sealing means are usually removed before the optical analysis and, if necessary, subsequently reapplied for transport or further treatment, which, however, is cumbersome and time-consuming, especially when removing films.
- evaporation and contamination effects during the measuring process cannot be excluded.
- the invention is based on the object of creating a multiwell analysis plate which can be handled easily and with little effort and which can also be used for fractions in the Microliter scale and analytical optical measurements, especially at elevated temperatures and over a longer period of time, largely falsified analysis results.
- the collecting vessel openings of the multiwell analysis plate are closed with an optically permeable sealing material, for example a transparent sealing film made of plastic or a glass plate, the adhesives for fastening the sealing material covering the collecting vessel openings being exclusively at the contact points of the sealing material with the multiwell analysis plate, but not in the area of the collecting vessel openings.
- an optically permeable sealing material for example a transparent sealing film made of plastic or a glass plate
- the adhesives for fastening the sealing material covering the collecting vessel openings being exclusively at the contact points of the sealing material with the multiwell analysis plate, but not in the area of the collecting vessel openings.
- Fig. 1 Multiwell analysis plate made of base body and sealing film in a perspective view
- Fig. 2 Basic body and sealing film in several views 1 shows a perspective view of a multiwell analysis plate with a base body 1, which has openings 2 in collecting vessels for fractionating, analyzing, storing and transporting substances, etc. So that the openings 2 are sealed against evaporation and contamination protection, a flexible, translucent sealing film 3 is provided, which is placed on the base body 1 and fixed by annular adhesive surfaces 4. The adhesive surfaces 4 are arranged around the openings 2 or the boundary edges of the base body 1. There is no adhesive layer itself on the translucent sealing film 3.
- the openings 2 of the receptacles are reliably closed, and when the sealing film 3 is applied, it is located exclusively on the contact surface between the sealing film 3 and the base body 1, ie on the edges of the openings 2 adhesive, but not in the area of the optical one Analysis analysis provided openings 2 of the collecting vessels.
- FIG. 2 shows the base body 1 and the sealing film 3 separately in different views and together in a side view, the base body 1 in cross section (FIG. 2a) and in a top view (FIG. 2b) and the sealing film 3 likewise in cross section (FIG. 2c ) and are shown in plan view (Fig. 2d).
- 2e shows the base body 1 closed with the sealing film 3 in cross section.
- the sealing film 3 consists, as in FIG. 1, of translucent material, with the difference that it is not the base body 1 but the sealing film 3 which has annular adhesive surfaces 5 or also has adhesive surfaces on the edges which correspond to adhesive contact surfaces 6 of the basic body 1 , These adhesive contact surfaces 6, like the adhesive surfaces 4 in FIG.
- the sealing film 3 In order to be able to apply the sealing film 3 with respect to the adhesive surfaces 5 and the position-assigned adhesive contact surfaces 6 on the base body 1 in an exact position, there are solitary structural elements 7 in the edge region of the base body 1 which engage in corresponding holes 8 in the sealing film 3.
- the adhesive on the adhesive surfaces 4 and 5 is preferably heat-soluble or hardenable by UV radiation.
Abstract
. According to the invention, the collecting-chamber openings of the multi-well analytical plate, generating largely reliable analytical results also at elevated temperatures and over a long period of time, are sealed with an optically clear sealing material, for example, clear plastic film, or a glass sheet. The adhesive for fixing the sealing material which covers the collecting-chamber openings is only applied to the contact area of the sealing material with the multi-well analytical plate and not to the area of the collecting-chamber opening. Neither the sealing material itself, nor the means of fixing to the multi-well analytical plate thereof, in particular, hot-melt adhesives impede the light flux from and to the samples for optical analytical determination.
Description
Verschlossene Multiwellanalysenplatte für analytische optische MessungenSealed multiwell analysis plate for analytical optical measurements
Die Erfindung betrifft eine verschlossene Multiwellanalysenplatte für analytische optische Messungen. In der Analytik, insbesondere für Biotechnologie, Biochemie, Umweltschutz und medizinische Diagnostik, hat sich in den letzten Jahren die sogenannte Multiwelltechnologie im großen Umfang durchgesetzt (beispielsweise Matthews P. D., Wurtzel E. T.: high-throughput microplate format for producing and screening riboprobes from bacterial cells, Biotechniques. 1995 Jun, 18 (6), 1000-1002, 1004 oder Wu P., Daniel-Issakani S., LaMarco K., Strulovici B.: an automated high throughput filtration assay: application to polymerase inhibitor Identification,; Anal-Biochem. 1997, Feb 15; 245 (2), 226-230 oder Rashed M. S., Bucknall M. P., Little D., Awad A., Jacob M., Alamoudi M., Alwattar M., Ozand P. T.: screening blood spots for inborn errors of metabolism by electrospray tandem mass spectrometry with a microplate batch process and a Computer algorithm for automated flagging of abnormal profiles, Clin-Chem. 1997, Jul; 43 (7), 1 129-1 141 ). Die Auswertung der meist optischen Signale, wie Absorbanz, Fluoreszens oder Lumineszens, erfolgt in der Regel mit geeigneten Readern in der oben offenen Multiwellanalysenplatte (z. B. Gerasimova N. S., Steklova I. V., Tuuminen T.: Fluorometric method for phenylalanine microplate assay adapted for phenylketonuria screening, Clin-Chem. 1989, Oct, 35 (10), 21 12-21 15 oder Collins L, et al. Microplate alamar blue assay versus BACTEC 460 System for high- throughput screening of compounds against Mycobacterium tuberculosis and Mycobacterium avium. Antimicrob Agents Chemother. 1997 May;41 (5): 1004-9. Herrero ME, et al. Fluorometric microassay to quantify microsomal epoxide hydrolase in 96-well plates. Anal Biochem. 1995 Sep 1 ;230(1 ):154-8, Barret JM, et al. Evaluation of DNA repair inhibition by antitumor or antibiotic drugs using a chemiluminescence microplate assay. Carcinogenesis. 1997 Dec;18(12):2441 -5.). Da diese Signale häufig erst nach dem Ablauf einer Vielzahl erforderlicher Analyseschritte, die auf chemischen, immunologischen oder enzymatischen Reaktionen beruhen, gewonnen werden, muß einer Verdunstung des
Analysengutes während dieses meist zeitaufwendigen Prozesses, insbesondere dann, wenn die Reaktionen bei erhöhten Temperaturen verlaufen, entgegengewirkt werden. Ein Verdunstungsschutz kann vollständig oder teilweise dadurch erreicht werden, indem die Multiwellanalysenplatten in eine feuchte Umgebung gestellt werden (Aufbewahrung häufig in feuchten Kammern), oder die Sammelgefäß- Öffnungen der Multiwellanalysenplatte sind durch Deckel oder Folien verschlossen (z. B. US 5 056 427, US 5 604 130). Als Folienmaterial finden Aluminium, Kunststoffe, aber auch elastisches Material welches durch geeignete Mittel auf die Ränder dichtend aufgepreßt wird, Verwendung (z. B. Firmenkataloge Greiner, Corning Costar, Millipore, INTERNET-Publikation der Firma Zymark zu ihrem Presto Automated Microplate Sealer). Sehr häufig werden die dichtenden Folien, die auf ihrer Rückfläche mit Kleber beschichtet sind, aufgeklebt und können mit geeigneten Ansatzpunkten wieder gelöst werden (z. B. Internet Publikation der Firma Advanced Biotechnologies). Ein entscheidender Nachteil dieser Abdeckungen der Multiwellanalysenplatten ist allerdings die Beeinträchtigung des Lichtdurchgangs bei optischen Messungen. Die Strahlung bzw. die ausgewerteten optischen Signale werden vollständig oder partiell durch das Dichtmaterial unterdrückt; zumindest kommt es aber über die Vielzahl der einzelnen Sammelgefäß-Öffnungen der Multiwellanalysenplatte zu inhomogenen Signalbeeinflussungen. Aus diesem Grund werden die dichtenden Mittel gewöhnlich vor der optischen Analyse entfernt und ggf. anschließend für den Transport bzw. die Weiterbehandlung neu aufgebracht, was jedoch, speziell beim Ablösen von Folien umständlich und mit Aufwand verbunden ist. Bei Entfernung der Dichtmittel von den Sammelgefäßen können wiederum Verdunstungs- und Verunreinigungseffekte während des Meßprozesses nicht ausgeschlossen werden. Diese führen, insbesondere bei der Meßwertanalyse von sehr geringen Probenvolumina, bei der Messung unter erhöhten Temperaturen sowie bei Messungen über einen längeren Zeitraum zwangsläufig zu störenden Meßwertverfälschungen.The invention relates to a closed multiwell analysis plate for analytical optical measurements. In analytics, especially for biotechnology, biochemistry, environmental protection and medical diagnostics, so-called multiwell technology has become widely used in recent years (e.g. Matthews PD, Wurtzel ET: high-throughput microplate format for producing and screening riboprobes from bacterial cells, Biotechniques. 1995 Jun, 18 (6), 1000-1002, 1004 or Wu P., Daniel-Issakani S., LaMarco K., Strulovici B .: an automated high throughput filtration assay: application to polymerase inhibitor identification ,; anal- Biochem. 1997, Feb 15; 245 (2), 226-230 or Rashed MS, Bucknall MP, Little D., Awad A., Jacob M., Alamoudi M., Alwattar M., Ozand PT: screening blood spots for inborn errors of metabolism by electrospray tandem mass spectrometry with a microplate batch process and a Computer algorithm for automated flagging of abnormal profiles, Clin-Chem. 1997, Jul; 43 (7), 1 129-1 141). The mostly optical signals, such as absorbance, fluorescence or luminescence, are usually evaluated with suitable readers in the open multiwell analysis plate (e.g. Gerasimova NS, Steklova IV, Tuuminen T .: Fluorometric method for phenylalanine microplate assay adapted for phenylketonuria screening, Clin-Chem. 1989, Oct, 35 (10), 21 12-21 15 or Collins L, et al. Microplate alamar blue assay versus BACTEC 460 System for high-throughput screening of compounds against Mycobacterium tuberculosis and Mycobacterium avium. Antimicrob Agents Chemother. 1997 May; 41 (5): 1004-9. Herrero ME, et al. Fluorometric microassay to quantify microsomal epoxide hydrolase in 96-well plates. Anal Biochem. 1995 Sep 1; 230 (1): 154-8, Barret JM, et al. Evaluation of DNA repair inhibition by antitumor or antibiotic drugs using a chemiluminescence microplate assay. Carcinogenesis. 1997 Dec; 18 (12): 2441 -5.). Since these signals are often only obtained after a large number of required analysis steps based on chemical, immunological or enzymatic reactions have taken place, evaporation of the Analytical goods are counteracted during this mostly time-consuming process, especially when the reactions take place at elevated temperatures. Evaporation protection can be achieved in whole or in part by placing the multiwell analysis plates in a moist environment (storage often in moist chambers), or the collecting vessel openings of the multiwell analysis plate are closed by lids or foils (e.g. US Pat. No. 5,056,427 US 5,604,130). Aluminum, plastics, but also elastic material, which is pressed onto the edges by suitable means, are used as film material (e.g. company catalogs Greiner, Corning Costar, Millipore, INTERNET publication from Zymark for their Presto Automated Microplate Sealer). Very often, the sealing foils, which are coated with glue on the back surface, are glued on and can be removed again with suitable starting points (e.g. Internet publication from Advanced Biotechnologies). A decisive disadvantage of these covers of the multiwell analysis plates is, however, the impairment of the passage of light during optical measurements. The radiation or the evaluated optical signals are completely or partially suppressed by the sealing material; at least there is inhomogeneous signal interference via the large number of individual collecting vessel openings in the multiwell analysis plate. For this reason, the sealing means are usually removed before the optical analysis and, if necessary, subsequently reapplied for transport or further treatment, which, however, is cumbersome and time-consuming, especially when removing films. When removing the sealant from the collecting vessels, evaporation and contamination effects during the measuring process cannot be excluded. These lead, particularly when analyzing measured values of very small sample volumes, when measuring at elevated temperatures and when measuring over a longer period of time, inevitably lead to distorted measured values.
Der Erfindung liegt die Aufgabe zu Grunde, eine gut und mit geringem Aufwand handhabbare Multiwellanalysenplatte zu schaffen, die auch für Fraktionen im
Mikrolitermaßstab sowie bei analytischen optischen Messungen, insbesondere bei erhöhten Temperaturen und über einen längeren Zeitraum, weitgehend unverfälschte Analyseergebnisse ermöglicht.The invention is based on the object of creating a multiwell analysis plate which can be handled easily and with little effort and which can also be used for fractions in the Microliter scale and analytical optical measurements, especially at elevated temperatures and over a longer period of time, largely falsified analysis results.
Erfindungsgemäß sind die Sammelgefäß-Öffnungen der Multiwellanalysenplatte mit einem optisch durchlässigen Dichtmaterial, beispielsweise einer durchsichtigen Dichtfolie aus Kunststoff oder einer Glasplatte, verschlossen, wobei sich die Haftmittel zur Befestigung des die Sammelgefäß-Öffnungen abdeckenden Dichtmaterials ausschließlich an den Berührungsstellen des Dichtmaterials zur Multiwellanalysenplatte, nicht aber im Bereich der Sammelgefäß-Öffnungen befinden. Auf diese Weise beeinträchtigen weder das Dichtmaterial selbst noch dessen Befestigungsmittel auf der Multiwellanalysenplatte, beispielsweise Haftkleber für Folien, nennenswert die Lichtstrahlung von und zu den Proben für die optische Analysenmessung. Die Strahlung kann weitgehend unverfälscht ausgewertet werden, obgleich die Sammelgefäße der Multiwellanalysenplatte verdunstungs- und kontaminierungssicher abgedeckt sowie die mitunter seltenen und kostbaren Proben sicher und geschützt aufbewahrt sind. Die Verdunstungs- und Verunreinigungsgefahr, welche besonders bei sehr kleinen Probenvolumina in der Praxis zu erheblichen Fehlauswertungen führen kann, wird damit nahezu ausgeschlossen. Der Aufwand für ein Entfernen und ggf. Neuaufbringen von Abdeckungen der Multiwellanalysenplatte entfällt. Das Analysegerät wird gegen aus den Proben austretende Substanzen geschützt.According to the invention, the collecting vessel openings of the multiwell analysis plate are closed with an optically permeable sealing material, for example a transparent sealing film made of plastic or a glass plate, the adhesives for fastening the sealing material covering the collecting vessel openings being exclusively at the contact points of the sealing material with the multiwell analysis plate, but not in the area of the collecting vessel openings. In this way, neither the sealing material itself nor its fastening means on the multiwell analysis plate, for example pressure-sensitive adhesive for foils, appreciably impair the light radiation from and to the samples for the optical analysis measurement. The radiation can be evaluated largely unadulterated, although the collecting vessels of the multiwell analysis plate are protected against evaporation and contamination, and the sometimes rare and valuable samples are kept safe and protected. The risk of evaporation and contamination, which can lead to considerable false evaluations in practice, especially with very small sample volumes, is almost eliminated. The effort for removing and possibly reapplying covers of the multiwell analysis plate is eliminated. The analyzer is protected against substances emerging from the samples.
In den Unteransprüchen 2 bis 13 sind vorteilhafte ausgestaltende Merkmale zur Erfindung angeführt.Advantageous design features for the invention are set out in subclaims 2 to 13.
Die Erfindung soll nachstehend anhand eines in der Zeichnung dargestelltenThe invention will now be described with reference to one in the drawing
Ausführungsbeispiels näher erläutert werden.Embodiment will be explained in more detail.
Es zeigen:Show it:
Fig. 1 : Multiwellanalysenplatte aus Grundkörper und Dichtfolie in perspektivischer AnsichtFig. 1: Multiwell analysis plate made of base body and sealing film in a perspective view
Fig. 2: Grundkörper und Dichtfolie in mehreren Ansichten
Fig. 1 zeigt in perspektivischer Ansicht eine Multiwellanalysenplatte mit einem Grundkörper 1 , der Öffnungen 2 von Sammelgefäßen zum Fraktionieren, Analysieren, Aufbewahren und Transportieren etc. von Substanzen aufweist. Damit die Öffnungen 2 verdunstungs- und kontaminierungsgeschützt abgedichtet werden, ist eine flexible, lichtdurchlässige Dichtfolie 3 vorgesehen, welche auf den Grundkörper 1 aufgelegt und durch ringförmige Klebeflächen 4 fixiert wird. Die Klebeflächen 4 sind um die Öffnungen 2 bzw. die Begrenzungskanten des Grundkörpers 1 herum angeordnet. An der lichtdurchlässigen Dichtfolie 3 befindet sich selbst keine haftende Schicht. Auf diese Weise sind die Öffnungen 2 der Sammelgefäße zuverlässig verschlossen, und es befindet sich beim Aufbringen der Dichtfolie 3 ausschließlich an der Berührungsfläche zwischen der Dichtfolie 3 und dem Grundkörper 1 , d. h. an den Rändern der Öffnungen 2 Klebstoff, nicht jedoch im Bereich der für optische Analysenauswertung vorgesehenen Öffnungen 2 der Sammelgefäße.Fig. 2: Basic body and sealing film in several views 1 shows a perspective view of a multiwell analysis plate with a base body 1, which has openings 2 in collecting vessels for fractionating, analyzing, storing and transporting substances, etc. So that the openings 2 are sealed against evaporation and contamination protection, a flexible, translucent sealing film 3 is provided, which is placed on the base body 1 and fixed by annular adhesive surfaces 4. The adhesive surfaces 4 are arranged around the openings 2 or the boundary edges of the base body 1. There is no adhesive layer itself on the translucent sealing film 3. In this way, the openings 2 of the receptacles are reliably closed, and when the sealing film 3 is applied, it is located exclusively on the contact surface between the sealing film 3 and the base body 1, ie on the edges of the openings 2 adhesive, but not in the area of the optical one Analysis analysis provided openings 2 of the collecting vessels.
Fig. 2 zeigt den Grundkörper 1 und die Dichtfolie 3 separat in unterschiedlichen Ansichten sowie gemeinsam in Seitenansicht, wobei der Grundkörper 1 im Querschnitt (Fig. 2a) und in Draufsicht (Fig. 2b) sowie die Dichtfolie 3 ebenfalls im Querschnitt (Fig. 2c) und in Draufsicht (Fig. 2d) dargestellt sind. Fig. 2e zeigt den mit der Dichtfolie 3 verschlossenen Grundkörper 1 im Querschnitt. Die Dichtfolie 3 besteht, wie in Fig. 1 , aus lichtdurchlässigem Material, mit dem Unterschied, daß nicht der Grundkörper 1 , sondern die Dichtfolie 3 ringförmige Klebeflächen 5 aufweist bzw. auch an den Kanten Klebeflächen besitzt, die mit Klebekontaktflächen 6 des Grundkörpers 1 korrespondieren. Diese Klebe- kontaktflächen 6 sind, wie die Klebeflächen 4 in Fig. 1 , ringförmig um die Öffnungen 2 der Sammelgefäße vom Grundkörper 1 herum angeordnet. Der Klebstoff zur Haftung der Dichtfolie 3 befindet sich wiederum ausschließlich im Bereich der Berührungsfläche zwischen der Dichtfolie 3 und dem Grundkörper 1. Die Bereiche der Öffnungen 2 sind frei von Haftmittel, welches ansonsten die
Öffnungen 2 durchdringende Lichtstrahlung für optische Analysenauswertungen beeinträchtigen würde.2 shows the base body 1 and the sealing film 3 separately in different views and together in a side view, the base body 1 in cross section (FIG. 2a) and in a top view (FIG. 2b) and the sealing film 3 likewise in cross section (FIG. 2c ) and are shown in plan view (Fig. 2d). 2e shows the base body 1 closed with the sealing film 3 in cross section. The sealing film 3 consists, as in FIG. 1, of translucent material, with the difference that it is not the base body 1 but the sealing film 3 which has annular adhesive surfaces 5 or also has adhesive surfaces on the edges which correspond to adhesive contact surfaces 6 of the basic body 1 , These adhesive contact surfaces 6, like the adhesive surfaces 4 in FIG. 1, are arranged in a ring around the openings 2 of the collecting vessels from the base body 1. The adhesive for adhering the sealing film 3 is again located exclusively in the area of the contact surface between the sealing film 3 and the base body 1. The areas of the openings 2 are free of adhesive, which otherwise the Openings 2 penetrating light radiation for optical analysis evaluations would impair.
Um die Dichtfolie 3 hinsichtlich der Klebeflächen 5 und der lagezugeordneten Klebekontaktflächen 6 positionsgenau auf dem Grundkörper 1 aufbringen zu können, sind im Randbereich des Grundkörpers 1 solitäre Strukturelemente 7 vorhanden, die in korrespondierende Löcher 8 der Dichtfolie 3 eingreifen. Der Klebstoff an den Klebeflächen 4 und 5 ist vorzugsweise wärmelösbar bzw. durch UV-Strahlung verhärtbar.
In order to be able to apply the sealing film 3 with respect to the adhesive surfaces 5 and the position-assigned adhesive contact surfaces 6 on the base body 1 in an exact position, there are solitary structural elements 7 in the edge region of the base body 1 which engage in corresponding holes 8 in the sealing film 3. The adhesive on the adhesive surfaces 4 and 5 is preferably heat-soluble or hardenable by UV radiation.
Aufstellung der verwendeten BezugszeichenList of the reference symbols used
1 Grundkörper der Multiwellanalysenplatte1 basic body of the multiwell analysis plate
2 Öffnung der Sammelgefäße2 Opening the collecting vessels
3 Dichtfolie 4, 5 Klebefläche3 sealing foil 4, 5 adhesive surface
6 Klebekontaktfläche6 adhesive contact surface
7 Strukturelement7 structural element
8 Loch
8 holes
Claims
1. Verschlossene Multiwellanalysenplatte für analytische optische Messungen, bestehend aus einem Grundkörper, der in einem definiertem X-Y Raster eine Vielzahl von Sammelgefäßen trägt, deren Öffnungen jeweils von einem in einer1. Sealed multiwell analysis plate for analytical optical measurements, consisting of a base body which carries a plurality of collecting vessels in a defined X-Y grid, the openings of which are each one in one
Ebene liegenden Rand begrenzt werden, und bestehend aus einer Abdeckung für die Öffnungen der Sammelgefäße, dadurch gekennzeichnet, daß die Öffnungen (2) der Sammelgefäße durch ein für das Licht der optischen Messungen durchlässiges und im Bereich der Öffnungen (2) nicht mit Haftmitteln versehenes Dichtmaterial (3) als Abdeckung verdunstungs- und kontaminierungsgeschützt verschlossen sind und daß die Haftmittel (4, 5) zur Befestigung des Dichtmaterials (3) ausschließlich im Bereich der als Berührungsfläche wirkenden Ränder zwischen den Öffnungen (2) der Sammelgefäße vorgesehen sind.Flat edge are limited, and consisting of a cover for the openings of the collecting vessels, characterized in that the openings (2) of the collecting vessels by a sealing material which is permeable to the light of the optical measurements and is not provided with adhesives in the region of the openings (2) (3) are sealed as a cover against evaporation and contamination and that the adhesives (4, 5) for fastening the sealing material (3) are provided exclusively in the area of the edges acting as a contact surface between the openings (2) of the collecting vessels.
2. Verschlossene Multiwellanalysenplatte nach Anspruch 1 , dadurch gekennzeichnet, daß als Haftmittel zur Befestigung des Dichtmaterials (3) auf dem Grundkörper (1 ) Klebstoff (4) verwendet wird, der an den Rändern der Öffnungen (2) auf dem Grundkörper (1 ) aufgebracht ist.2. Closed multiwell analysis plate according to claim 1, characterized in that adhesive (4) is used as an adhesive for fastening the sealing material (3) on the base body (1), which is applied to the edges of the openings (2) on the base body (1) is.
3. Verschlossene Multiwellanalysenplatte nach Anspruch 1 , dadurch gekennzeichnet, daß als Haftmittel zur Befestigung des Dichtmaterials (3) auf dem Grundkörper (1 ) Klebstoff (5) verwendet wird, der im Muster der Ränder der im definierten X-Y Raster angeordneten Öffnungen (2) des Grundkörpers (1) auf dem Dichtmaterial (3) aufgebracht ist.3. Closed multiwell analysis plate according to claim 1, characterized in that adhesive (5) is used as an adhesive for fastening the sealing material (3) on the base body (1), which is arranged in the pattern of the edges of the openings (2) defined in the defined XY grid Base body (1) on the sealing material (3) is applied.
4. Verschlossene Multiwellanalysenplatte nach Anspruch 1 und/oder 2 dadurch gekennzeichnet, daß der Klebstoff (4, 5) wärmelösbar ist. 4. Sealed multiwell analysis plate according to claim 1 and / or 2, characterized in that the adhesive (4, 5) is heat-soluble.
5. Verschlossene Multiwellanalysenplatte nach Anspruch 1 und/oder 2 dadurch gekennzeichnet, daß der Klebstoff (4, 5) mit UV-Strahlung verhärtbar ist.5. Sealed multiwell analysis plate according to claim 1 and / or 2, characterized in that the adhesive (4, 5) can be hardened with UV radiation.
6. Verschlossene Multiwellanalysenplatte nach Anspruch 4, dadurch gekennzeichnet, daß als wärmelösbarer Klebstoff ein Wachs mit Schmelztemperaturen zwischen 90 °C und 150 °C vorgesehen ist.6. Sealed multiwell analysis plate according to claim 4, characterized in that a wax with melting temperatures between 90 ° C and 150 ° C is provided as a heat-releasable adhesive.
7. Verschlossene Multiwellanalysenplatte nach Anspruch 1 , dadurch gekennzeichnet, daß als Dichtmaterial eine durchsichtige Folie (3) vorgesehen ist.7. Sealed multiwell analysis plate according to claim 1, characterized in that a transparent film (3) is provided as the sealing material.
8. Verschlossene Multiwellanalysenplatte nach Anspruch 6, dadurch gekennzeichnet, daß die Folie (3) aus Kunststoff besteht.8. Closed multiwell analysis plate according to claim 6, characterized in that the film (3) consists of plastic.
9. Verschlossene Multiwellanalysenplatte nach Anspruch 1 , dadurch gekennzeichnet, daß als Dichtmaterial eine Glasplatte verwendet wird.9. Sealed multiwell analysis plate according to claim 1, characterized in that a glass plate is used as sealing material.
10. Verschlossene Multiwellanalysenplatte nach Anspruch 1 , dadurch gekennzeichnet, daß auf dem Grundkörper (1 ) Mittel vorgesehen sind, die eine exakte10. Closed multiwell analysis plate according to claim 1, characterized in that means are provided on the base body (1) which are exact
Lagezuordnung des Dichtmaterials (3) zum Grundkörper (1 ) hinsichtlich der Klebeflächen (4, 5) im Bereich der als Berührungsfläche wirkenden Ränder zwischen den Öffnungen (2) der Sammelgefäße gewährleisten.Ensure the positional assignment of the sealing material (3) to the base body (1) with regard to the adhesive surfaces (4, 5) in the region of the edges acting as a contact surface between the openings (2) of the collecting vessels.
1 1 . Verschlossene Multiwellanalysenplatte nach Anspruch 9, dadurch gekennzeichnet, daß die Mittel zur Lagezuordnung aus, vorzugsweise im äußeren Begrenzungsbereich, auf dem Grundkörper (1 ) angeordneten, Strukturelementen, wie solitäre Erhöhungen (7), bestehen, die zu Strukturelementen, wie Löcher (8), im Dichtmaterial (3) korrespondieren.1 1. Sealed multiwell analysis plate according to claim 9, characterized in that the means for location assignment consist, preferably in the outer boundary area, of structural elements, such as solitary elevations (7), arranged on the base body (1), which correspond to structural elements such as holes (8) in the sealing material (3).
12. Verschlossene Multiwellanalysenplatte nach Anspruch 1 , dadurch gekennzeichnet, daß der Grundkörper (1 ) und das Dichtmaterial (3) miteinander verschweißt sind.12. Closed multiwell analysis plate according to claim 1, characterized in that the base body (1) and the sealing material (3) are welded together.
13. Verschlossene Multiwellanalysenplatte nach Anspruch 1 , dadurch gekennzeichnet, daß das ansonsten optisch durchlässige Dichtmaterial (3) im Infrarotbereich absorbiert und, vorzugsweise durch einen Infrarotstrahler, erwärmt wird. 13. Sealed multiwell analysis plate according to claim 1, characterized in that the otherwise optically permeable sealing material (3) is absorbed in the infrared region and is heated, preferably by an infrared radiator.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/EP2000/001003 WO2001058591A2 (en) | 2000-02-08 | 2000-02-08 | Closed multi-well analytical plate with annular adhesive patches for analytical optical measurements |
| AU2000229083A AU2000229083A1 (en) | 2000-02-08 | 2000-02-08 | Closed multi-well analytical plate with annular adhesive patches for analytical optical measurements |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/EP2000/001003 WO2001058591A2 (en) | 2000-02-08 | 2000-02-08 | Closed multi-well analytical plate with annular adhesive patches for analytical optical measurements |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO2001058591A2 true WO2001058591A2 (en) | 2001-08-16 |
| WO2001058591A3 WO2001058591A3 (en) | 2002-10-31 |
Family
ID=8163825
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/EP2000/001003 WO2001058591A2 (en) | 2000-02-08 | 2000-02-08 | Closed multi-well analytical plate with annular adhesive patches for analytical optical measurements |
Country Status (2)
| Country | Link |
|---|---|
| AU (1) | AU2000229083A1 (en) |
| WO (1) | WO2001058591A2 (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102004038152A1 (en) * | 2004-08-06 | 2006-02-23 | Eppendorf Ag | Covering film for microtiter plate, for use e.g. in microbiology or cell culture, comprises weakened zones over the wells to facilitate access while retaining protection against evaporation and cross-contamination |
| EP1937410A1 (en) * | 2005-10-18 | 2008-07-02 | GE Healthcare Bio-Sciences AB | Multiwell plate |
| EP2040983A2 (en) * | 2006-06-26 | 2009-04-01 | Applera Corporation | Compressible transparent sealing for open microplates |
| JP2011047754A (en) * | 2009-08-26 | 2011-03-10 | Shimadzu Corp | Reaction vessel |
| WO2012010412A1 (en) * | 2010-07-19 | 2012-01-26 | Boehringer Ingelheim Microparts Gmbh | Microfluidic device and method for producing same |
| EP2446967A1 (en) * | 2010-11-02 | 2012-05-02 | F. Hoffmann-La Roche AG | System for processing and/or analyzing liquid samples, sealing arrangement and method for heat-sealing a microplate |
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|---|---|---|---|---|
| US4444310A (en) * | 1982-07-26 | 1984-04-24 | Becton, Dickinson And Company | Segmented multi-product package assembly |
| WO1992020448A1 (en) * | 1991-05-15 | 1992-11-26 | Packard Instrument Company, Inc. | Microplate for containment of radioactive samples |
| WO1994012405A2 (en) * | 1992-12-03 | 1994-06-09 | Techne (Cambridge) Limited | Closure means, containers and methods of closure |
| US5763263A (en) * | 1995-11-27 | 1998-06-09 | Dehlinger; Peter J. | Method and apparatus for producing position addressable combinatorial libraries |
| DE19852946A1 (en) * | 1998-11-12 | 2000-05-18 | Univ Schiller Jena | Micro-titration plate has transparent cover adhered between cell openings, to prevent contamination and evaporation whilst avoiding interference with optical analysis beams |
-
2000
- 2000-02-08 WO PCT/EP2000/001003 patent/WO2001058591A2/en active Application Filing
- 2000-02-08 AU AU2000229083A patent/AU2000229083A1/en not_active Abandoned
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4444310A (en) * | 1982-07-26 | 1984-04-24 | Becton, Dickinson And Company | Segmented multi-product package assembly |
| WO1992020448A1 (en) * | 1991-05-15 | 1992-11-26 | Packard Instrument Company, Inc. | Microplate for containment of radioactive samples |
| WO1994012405A2 (en) * | 1992-12-03 | 1994-06-09 | Techne (Cambridge) Limited | Closure means, containers and methods of closure |
| US5763263A (en) * | 1995-11-27 | 1998-06-09 | Dehlinger; Peter J. | Method and apparatus for producing position addressable combinatorial libraries |
| DE19852946A1 (en) * | 1998-11-12 | 2000-05-18 | Univ Schiller Jena | Micro-titration plate has transparent cover adhered between cell openings, to prevent contamination and evaporation whilst avoiding interference with optical analysis beams |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102004038152A1 (en) * | 2004-08-06 | 2006-02-23 | Eppendorf Ag | Covering film for microtiter plate, for use e.g. in microbiology or cell culture, comprises weakened zones over the wells to facilitate access while retaining protection against evaporation and cross-contamination |
| DE102004038152B4 (en) * | 2004-08-06 | 2006-06-08 | Eppendorf Ag | Covering film for microtiter plate, for use e.g. in microbiology or cell culture, comprises weakened zones over the wells to facilitate access while retaining protection against evaporation and cross-contamination |
| EP1937410A1 (en) * | 2005-10-18 | 2008-07-02 | GE Healthcare Bio-Sciences AB | Multiwell plate |
| EP1937410A4 (en) * | 2005-10-18 | 2014-05-21 | Ge Healthcare Bio Sciences Ab | Multiwell plate |
| EP2040983A2 (en) * | 2006-06-26 | 2009-04-01 | Applera Corporation | Compressible transparent sealing for open microplates |
| EP2040983A4 (en) * | 2006-06-26 | 2011-08-03 | Life Technologies Corp | Compressible transparent sealing for open microplates |
| JP2011047754A (en) * | 2009-08-26 | 2011-03-10 | Shimadzu Corp | Reaction vessel |
| WO2012010412A1 (en) * | 2010-07-19 | 2012-01-26 | Boehringer Ingelheim Microparts Gmbh | Microfluidic device and method for producing same |
| EP2446967A1 (en) * | 2010-11-02 | 2012-05-02 | F. Hoffmann-La Roche AG | System for processing and/or analyzing liquid samples, sealing arrangement and method for heat-sealing a microplate |
Also Published As
| Publication number | Publication date |
|---|---|
| AU2000229083A8 (en) | 2001-11-01 |
| AU2000229083A1 (en) | 2001-08-20 |
| WO2001058591A3 (en) | 2002-10-31 |
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