WO1992022669A1 - Reagent mixtures for glucose assay - Google Patents
Reagent mixtures for glucose assay Download PDFInfo
- Publication number
- WO1992022669A1 WO1992022669A1 PCT/GB1992/001116 GB9201116W WO9222669A1 WO 1992022669 A1 WO1992022669 A1 WO 1992022669A1 GB 9201116 W GB9201116 W GB 9201116W WO 9222669 A1 WO9222669 A1 WO 9222669A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- glucose oxidase
- reagent mixture
- ius
- test reagent
- mixture
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
Definitions
- the glucose oxidase is present in from 400 to 550 IUs per 27.5 to 32.5 IUs of peroxidase and the chromogen is o-tolidine which is present in an amount of from 12 to 15 gs per 500,000 IUs of the glucose oxidase.
- the reagent mixture be put up in a gel matrix, notably a gelatin matrix, which provides from 200 to 400 gs of the matrix on a dry weight basis per 500,000 IUs of the glucose oxidase.
- a gel matrix notably a gelatin matrix, which provides from 200 to 400 gs of the matrix on a dry weight basis per 500,000 IUs of the glucose oxidase.
- the reagent mixture may contain other materials as is customary, for example phosphate buffering agents, preservatives, anti-coagulants or surface active agents. Such other materials are typically inert to the material to be tested, the other constituents of the reagent mixture and the products of the reactions and interactions which occur during the test procedure. Such other constituents can be present in the amounts normally used in such reagent mixtures.
- the chromogenic material will typically be present in an amount of from 12 to 17, notably from 13 to 16, grams per 500,000 IUs of the glucose oxidase. The optimum proportions within these ranges can be determined for any given case and a given carrier by simple trial and error tests.
- the reagent mixture is preferably put up in a matrix carrier medium so that the material to be assessed can penetrate to the enzyme reagent and the chromogenic material.
- the matrix can be provided by a natural gum, jelly or gel, for example a gelatin, agar agar, aspic or silica gel; or can be provided by a synthetic polymer gel, for example a cellulosic gel or a polyvinylic resin gel.
- a gelatin gel as the carrier matrix.
- the matrix may contain the enzymatic reagent and chromogenic material non-uniformly distributed therein, as when a gel layer is formed which has a high concentration of the gel matrix in its upper layers to act a protective layer or coating for the reagent rich lower layers; or where the enzymatic reagent is located in a separate zone of the matrix from that containing the chromogenic material.
- the product from the interaction of the material being tested with one or more of the enzymatic reagents diffuses from the enzyme zone into the zone containing the chromogenic material to develop a colour as a separate stage in that zone.
- the matrix carrying the reagent mixture can be put up in a number of physical forms, for example as test strips or discs in which a pad of the matrix is applied to one face of the strip or disc and the colour resulting from the interaction of the material under assessment and the reagents and materials in the matrix is observed visually against the white background of the support strip or disc or against a separate reference background.
- the matrix can be put up in a series of zones through which the reagent mixture is distributed so that the interaction of the material to be assessed with the enzyme occurs in one zone and the product of that interaction diffuses to a second zone in which the colour reaction takes place.
- the reagent mixture matrix can be absorbed or impregnated into the pores of . a porous carrier to one face of which the material to be assessed is applied and the colour developing within the matrix is viewed from the opposite surface of the carrier.
- the gel matrix is a high molecular weight gelatin which is present in about 4% by dry weight.
- a low molecular weight gelatin typically with a molecular weight in the range 20,000 to 50,000.
- the gel content of the reagent mixture composition exceeds about 20% by dry weight, the gel inhibits the diffusion of reaction products through the membrane and hence development cf a colour reflecting the true extent of the interactions which have occurred.
- the resultant solution was impregnated into a polysulfone resin sheet (0.2 to 0.4 mms thick and having an average pore diameter of 0.2 micrometres and an air permeability of 3 litres per minute per square centimetre at an applied pressure of 10 psig) to provide 5 IUs of glucose oxidase, 3 IUs of peroxidase, 0.2 milligrams of o-tolidine and 4 milligrams of gelatin per square centimetre of the membrane.
- the same membrane was impregnated with a conventional reagent mixture to provide the conventional level of enzyme and chromogen per square centimetre.
Abstract
Description
Claims
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU20211/92A AU661492B2 (en) | 1991-06-19 | 1992-06-19 | Reagent mixtures for glucose assay |
EP92913166A EP0591322A1 (en) | 1991-06-19 | 1992-06-19 | Reagent mixtures for glucose assay |
JP5500799A JPH06510426A (en) | 1991-06-19 | 1992-06-19 | Glucose measurement reagent |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB919113212A GB9113212D0 (en) | 1991-06-19 | 1991-06-19 | Reagent mixture |
GB9113212.6 | 1991-06-19 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1992022669A1 true WO1992022669A1 (en) | 1992-12-23 |
Family
ID=10696932
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/GB1992/001116 WO1992022669A1 (en) | 1991-06-19 | 1992-06-19 | Reagent mixtures for glucose assay |
Country Status (7)
Country | Link |
---|---|
EP (1) | EP0591322A1 (en) |
JP (1) | JPH06510426A (en) |
AU (1) | AU661492B2 (en) |
CA (1) | CA2110908A1 (en) |
GB (1) | GB9113212D0 (en) |
HU (1) | HUT65982A (en) |
WO (1) | WO1992022669A1 (en) |
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1995031730A1 (en) * | 1994-05-18 | 1995-11-23 | Microquest Diagnostics, Inc. | Method of rapid analyte detection |
WO2012109473A2 (en) * | 2011-02-09 | 2012-08-16 | University Of Massachusetts | Detection of endotoxins |
US8859293B2 (en) | 2003-06-20 | 2014-10-14 | Roche Diagnostics Operations, Inc. | Method for determining whether a disposable, dry regent, electrochemical test strip is unsuitable for use |
US8877035B2 (en) | 2005-07-20 | 2014-11-04 | Bayer Healthcare Llc | Gated amperometry methods |
US9110013B2 (en) | 2005-09-30 | 2015-08-18 | Bayer Healthcare Llc | Gated voltammetry methods |
US9410917B2 (en) | 2004-02-06 | 2016-08-09 | Ascensia Diabetes Care Holdings Ag | Method of using a biosensor |
US9410915B2 (en) | 2004-06-18 | 2016-08-09 | Roche Operations Ltd. | System and method for quality assurance of a biosensor test strip |
US9933385B2 (en) | 2007-12-10 | 2018-04-03 | Ascensia Diabetes Care Holdings Ag | Method of using an electrochemical test sensor |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2000125994A (en) * | 1998-10-28 | 2000-05-09 | Aisin Seiki Co Ltd | Resin cushion elements |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB893318A (en) * | 1959-11-02 | 1962-04-04 | Miles Lab | Stable blood detecting composition |
FR2295425A1 (en) * | 1974-12-21 | 1976-07-16 | Boehringer Mannheim Gmbh | APPLICATION OF 3,3,5,5-TETRA-ALCOYLBENZIDINE DERIVATIVES AS OXIDATION INDICATORS IN RAPID TESTS |
EP0016947A1 (en) * | 1979-04-04 | 1980-10-15 | Roche Diagnostics GmbH | Process and reagent for the enzymatic determination of enzymatic substrates |
US4340669A (en) * | 1981-02-12 | 1982-07-20 | Miles Laboratories, Inc. | System for the determination of glucose in fluids |
-
1991
- 1991-06-19 GB GB919113212A patent/GB9113212D0/en active Pending
-
1992
- 1992-06-19 EP EP92913166A patent/EP0591322A1/en not_active Withdrawn
- 1992-06-19 WO PCT/GB1992/001116 patent/WO1992022669A1/en not_active Application Discontinuation
- 1992-06-19 CA CA 2110908 patent/CA2110908A1/en not_active Abandoned
- 1992-06-19 JP JP5500799A patent/JPH06510426A/en not_active Withdrawn
- 1992-06-19 HU HU9303638A patent/HUT65982A/en unknown
- 1992-06-19 AU AU20211/92A patent/AU661492B2/en not_active Ceased
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB893318A (en) * | 1959-11-02 | 1962-04-04 | Miles Lab | Stable blood detecting composition |
FR2295425A1 (en) * | 1974-12-21 | 1976-07-16 | Boehringer Mannheim Gmbh | APPLICATION OF 3,3,5,5-TETRA-ALCOYLBENZIDINE DERIVATIVES AS OXIDATION INDICATORS IN RAPID TESTS |
EP0016947A1 (en) * | 1979-04-04 | 1980-10-15 | Roche Diagnostics GmbH | Process and reagent for the enzymatic determination of enzymatic substrates |
US4340669A (en) * | 1981-02-12 | 1982-07-20 | Miles Laboratories, Inc. | System for the determination of glucose in fluids |
Cited By (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5750357A (en) * | 1994-05-18 | 1998-05-12 | Microquest Diagnostics, Inc. | Method of rapid analyte detection |
WO1995031730A1 (en) * | 1994-05-18 | 1995-11-23 | Microquest Diagnostics, Inc. | Method of rapid analyte detection |
US8859293B2 (en) | 2003-06-20 | 2014-10-14 | Roche Diagnostics Operations, Inc. | Method for determining whether a disposable, dry regent, electrochemical test strip is unsuitable for use |
US9410917B2 (en) | 2004-02-06 | 2016-08-09 | Ascensia Diabetes Care Holdings Ag | Method of using a biosensor |
US10067082B2 (en) | 2004-02-06 | 2018-09-04 | Ascensia Diabetes Care Holdings Ag | Biosensor for determining an analyte concentration |
US9410915B2 (en) | 2004-06-18 | 2016-08-09 | Roche Operations Ltd. | System and method for quality assurance of a biosensor test strip |
US8877035B2 (en) | 2005-07-20 | 2014-11-04 | Bayer Healthcare Llc | Gated amperometry methods |
US9835582B2 (en) | 2005-09-30 | 2017-12-05 | Ascensia Diabetes Care Holdings Ag | Devices using gated voltammetry methods |
US9110013B2 (en) | 2005-09-30 | 2015-08-18 | Bayer Healthcare Llc | Gated voltammetry methods |
US10670553B2 (en) | 2005-09-30 | 2020-06-02 | Ascensia Diabetes Care Holdings Ag | Devices using gated voltammetry methods |
US11435312B2 (en) | 2005-09-30 | 2022-09-06 | Ascensia Diabetes Care Holdings Ag | Devices using gated voltammetry methods |
US9933385B2 (en) | 2007-12-10 | 2018-04-03 | Ascensia Diabetes Care Holdings Ag | Method of using an electrochemical test sensor |
US10690614B2 (en) | 2007-12-10 | 2020-06-23 | Ascensia Diabetes Care Holdings Ag | Method of using an electrochemical test sensor |
WO2012109473A3 (en) * | 2011-02-09 | 2012-12-13 | University Of Massachusetts | Detection of endotoxins |
WO2012109473A2 (en) * | 2011-02-09 | 2012-08-16 | University Of Massachusetts | Detection of endotoxins |
Also Published As
Publication number | Publication date |
---|---|
HUT65982A (en) | 1994-08-29 |
EP0591322A1 (en) | 1994-04-13 |
AU661492B2 (en) | 1995-07-27 |
GB9113212D0 (en) | 1991-08-07 |
CA2110908A1 (en) | 1992-12-23 |
AU2021192A (en) | 1993-01-12 |
JPH06510426A (en) | 1994-11-24 |
HU9303638D0 (en) | 1994-04-28 |
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