US8765161B2

US8765161B2 - Monomers and phase-separated biocompatible polymer compositions prepared therefrom for medical uses

Info

Publication number: US8765161B2
Application number: US13/387,908
Authority: US
Inventors: Joachim B. Kohn; Durgadas Bolikal; Ramiro Rojas
Original assignee: Rutgers State University of New Jersey
Current assignee: Rutgers State University of New Jersey
Priority date: 2009-07-31
Filing date: 2010-07-31
Publication date: 2014-07-01
Also published as: EP2459638B1; EP2459638A1; EP2459638A4; EP2459617A4; US20120197001A1; WO2011014859A1; WO2011014860A1; EP2459637A1; WO2011014858A1; JP2013501107A; EP2459637A4; EP2459617A1; US8415449B2; US9080015B2; US20120189713A1; JP5944314B2; US20120178885A1; JP2013501106A; JP5956337B2

Abstract

The invention relates to novel monomers of Formula (I) useful for preparation of phase-separated biocompatible polymers or polymer compositions. These polymers or polymer compositions may be bioresorbable and/or biodegradable and have desirable mechanical properties, such as fracture and/or fatigue toughness, which have not been a primary design criteria for such polymers previously. The polymers or polymer compositions are useful in a variety of medical applications, such as in the fabrication of medical devices. Therefore, methods for preparing these polymers or polymer compositions and medical devices are also encompassed by this disclosure.

Description

This application is the U.S. National Phase of International Patent Application Serial No. PCT/US10/44052, filed on Jul. 31, 2010, which claims priority under 35 U.S.C. §119(e) to U.S. Provisional Patent Application Ser. No. 61/230,558, filed on Jul. 31, 2009, both of which are [[is]] hereby incorporated by reference in its entirety their entireties for all purposes.

STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH

The invention described herein was supported in whole or in part by grants from the National Institutes of Health (Grant No. EB001046). The U.S. Government has certain rights in this invention.

FIELD OF THE INVENTION

The present invention relates to new classes of monomeric compounds useful for preparation of biocompatible polymers and biocompatible polymers prepared therefrom, including novel biodegradable and/or bioresorbable polymers. These polymers, while not limited thereto, may be adapted for radio-opacity and are useful for medical device applications and controlled release therapeutic formulations.

BACKGROUND OF THE INVENTION

The rapidly evolving field of bioengineering has created a demand for a diverse library of different types of polymers offering a wide variety of choice of physical and mechanical properties. It is desirable that libraries of many different materials be available so that the specific polymer properties can be optimally matched with the requirements of the specific applications under development.

Examples of polymers suitable for various bioengineering applications include those described in U.S. Pat. Nos. 5,099,060; 5,665,831; 5,916,998 and 6,475,477, along with the polymers described in U.S. Patent Publication Nos. 2006/0024266 and 2006/0034769. There are numerous applications in which it is considered desirable for an implanted medical device to maintain its integrity and performance characteristics for extended periods of time, even under demanding mechanical conditions such as repeated mechanical flexure. Although many types of bioresorbable and/or biodegradable polymers are known, they are generally not selected for such applications because they were designed for temporary presence in the body and therefore lacked the desired combination of physical and mechanical properties. In addition, bioresorption and/or biodegradation tend to alter mechanical properties in unpredictable ways that are not necessarily linearly related to each other. Thus, there is a need for biocompatible, bioresorbable and/or biodegradable polymers having desirable mechanical properties, such as fracture and/or fatigue toughness, that have previously not been a primary design criteria for such polymers.

SUMMARY OF THE INVENTION

The present invention addresses the foregoing need by providing new monomers useful for the preparation of the desired biocompatible polymers and various type of such polymers useful for making the implantable medical devices. Various embodiments provide phase-separated polymer compositions, medical devices containing such compositions, and methods of using such polymer compositions and devices.

In one aspect the present invention provides novel monomers having the following generic structure (A):

wherein:

- R¹has the structure —R²—C(═O)—NR³—CHR⁴—R⁵—; X¹and X²are bromine or iodine; and y¹and y²have values independently selected from 0, 1, 2, 3 and 4;
- R²is a heteroalkyl group containing from one to eight carbon atoms and up to three heteroatoms independently selected from O, NR³and S;
- R³is hydrogen or a lower alkyl group containing from one to six carbon atoms;
- R⁴is COOR⁶, wherein R⁶is hydrogen or an alkyl, aryl, alkylaryl, heteroalkyl or heteroalkylaryl group containing up to 30 carbon atoms, wherein the heteroalkyl group contains from 1 to 10 heteroatoms independently selected from O, NR³and S and the heteroalkylaryl group contains from 1 to 3 heteroatoms independently selected from O, NR³and S; and
- R⁵is a bond or —CH₂—.

In this aspect the present invention provides new monomers having a structure of Formula (I):

wherein:

i is an integer selected from 1 through 4;

y¹and y²are each independently selected from 0, 1, 2, 3 and 4;

X¹and X², at each occurrence, are independently bromine (Br) or iodine (I);

X is oxygen (O), sulfur (S), or NR⁴, where R⁴is selected from the group consisting of hydrogen and alkyl containing from 1 to 6 carbon atoms;

R³is an optionally substituted C_1-30alkyl; and

R⁶is hydrogen or an alkyl, aryl, alkylaryl, heteroalkyl or heteroalkylaryl group containing up to 30 carbon atoms, wherein the heteroalkyl group contains from 1 to 10 heteroatoms independently selected from O, NR³and S and the heteroalkylaryl group contains from 1 to 3 heteroatoms independently selected from O, NR³and S;

wherein the —X¹and —OH groups on the left phenyl ring, at each occurrence, are independently at o-, m-, or p-positions.

In another aspect the present invention provides polymers, such as polycarbonates, polyarylates, polyiminocarbonates, polyphosphazenes and polyphosphoesters, comprising the repeating structure of Formula (B):

wherein X¹, X², y1, y2 and R¹, and the embodiments thereof, are the same as described above with respect to Formula I and A¹is selected from:

wherein R¹⁰is selected from H, C₁-C₃₀alkyl, alkenyl or alkynyl and C₂-C₃₀heteroalkyl; heteroalkenyl or heteroalkynyl, and R¹²is selected from C₁-C₃₀alkyl, alkenyl or alkynyl, C₁-C₃₀heteroalkyl; heteroalkenyl or heteroalkynyl, C₅-C₃₀heteroalkylaryl, heteroalkenylary or heteroalkynylaryl, C₆-C₃₀alkylaryl, alkenylaryl or alkynylaryl, and C₅-C₃₀heteroaryl.

In another aspect the present invention provides a biocompatible polymer composition, comprising at least a first polymer phase and a second polymer phase;

the first polymer phase having at least one first wet thermal transition temperature selected from a first wet glass transition temperature and a first wet melting point, the first wet thermal transition temperature being at least 38° C.;

the first polymer phase comprising a number (n) of first recurring units of Formula (I-2):

wherein:

i is an integer selected from 1 through 4;

y¹and y²are each independently selected from 0, 1, 2, 3 and 4;

X¹and X², at each occurrence, are independently bromine (Br) or iodine (I);

X is oxygen (O), sulfur (S), or NR⁴, where R⁴is selected from the group consisting of hydrogen and alkvl containing from 1 to 6 carbon atoms;

R³is an optionally substituted C_1-30alkyl;

R⁶is hydrogen or an alkyl, aryl, alkylaryl, heteroalkyl or heteroalkylaryl group containing up to 30 carbon atoms, wherein the heteroalkyl group contains from 1 to 10 heteroatoms independently selected from O, NR³and S and the heteroalkylaryl group contains from 1 to 3 heteroatoms independently selected from O, NR³and S; and

A¹is selected from:

R¹⁰is selected from H, C₁-C₃₀alkyl, alkenyl or alkynyl and C₂-C₃₀heteroalkyl; heteroalkenyl or heteroalkynyl; and

R¹²is selected from C₁-C₃₀alkyl, alkenyl or alkynyl, C₁-C₃₀heteroalkyl; heteroalkenyl or heteroalkynyl, C₅-C₃₀heteroalkylaryl, heteroalkenylary or heteroalkynylaryl, C₆-C₃₀alkylaryl, alkenylaryl or alkynylaryl, and C₅-C₃₀heteroaryl;

wherein the —X¹and —O— groups on the left phenyl ring, at each occurrence, are independently at o-, m-, or p-positions;

the second polymer phase having at least one second wet thermal transition temperature selected from a second wet glass transition temperature and a second wet melting point, the second wet thermal transition temperature being 36° C. or lower, the second polymer phase comprising a number (m) of second recurring units;

wherein the number (n) and the number (m) are selected to control the relative amounts of the first polymer phase and the second polymer phase so that (a) the polymer composition is phase-separated over at least the temperature range of about 25° C. to about 50° C., (b) the polymer composition has a water content of 4.5% or less as measured after soaking for 24 hours at 37° C. in 0.1 M phosphate buffered saline (PBS) at pH 7.4; and (c) the volume fraction of the second polymer phase in the polymer composition is in the range of about 6% to about 40%, based on total volume.

In another aspect the present invention provides a biocompatible polymer composition, comprising:

a number (n) of first polymer recurring unit of Formula (I-2):

wherein:

i is an integer selected from 1 through 4;

y¹and y²are each independently selected from 0, 1, 2, 3 and 4;

X¹and X², at each occurrence, are independently bromine (Br) or iodine (I);

R³is an optionally substituted C_1-30alkyl;

A¹is selected from:

wherein the —X¹and —O— groups on the left phenyl ring, at each occurrence, are independently at o-, m-, or p-positions; and

a number (m) of second polymer recurring units, effective to result in phase separation of said polymer composition into first and second polymer phases, wherein said second phase comprises said second polymer recurring units;

wherein the polymer composition has a water content of 4.5% or less as measured after soaking for 24 hours at 37° C. in 0.1 M phosphate buffered saline (PBS) at pH 7.4; and the second polymer recurring units and the number values for (n) and (m) are selected so that said polymer composition remains intact for at least about 15 minutes when tested under fatigue test conditions that comprise (i) providing a fatigue test strip having measurements of 5.0 mm wide, a gauge length of 15 mm and a thickness of 0.1 mm, (ii) aging the fatigue test strip for 7 days at 37° C. in 0.1 M phosphate buffered saline (PBS) at pH 7.4, and (iii) subjecting the aged fatigue test strip to oscillating deformation at a frequency of 1.2 Hz under a stress of 10 MPa in single frequency stress mode while submerged in water at 37° C.

Another embodiment provides a polymer compositions as described above, wherein the second recurring units have a formula selected from the group consisting of the formula (IIa), the formula (IIb), the formula (IIc), and the formula (IId):

wherein X³, X⁴, X⁵, X⁷, X⁸, X⁹, X¹⁰, X¹¹, X¹²and X¹³are independently selected from the group consisting of O, S and NR¹⁰, where R¹⁰is selected from hydrogen and an alkyl group containing from one to 30 carbon atoms;
Ar¹and Ar²are phenyl rings optionally substituted with from one to four substituents independently selected from the group consisting of a halogen, a halomethyl, a halomethoxy, a methyl, a methoxy, a thiomethyl, a nitro, a sulfoxide, and a sulfonyl;
R⁸and R⁹contain from one to ten carbon atoms each and are independently selected from the group consisting of an optionally substituted alkylene, an optionally substituted heteroalkylene, an optionally substituted alkenylene, and an optionally substituted heteroalkenylene;
g and h in formula (IId) are each independently integers in the range of about 1 to about 500; and
D and D¹contain up to 24 carbon atoms and are independently selected from the group consisting of an optionally substituted alkylene, an optionally substituted heteroalkylene, an optionally substituted alkenylene and an optionally substituted heteroalkenylene;
or D, X⁸and X⁹in formula (IIa) are selected so that HX⁸-D-X⁹H defines a hydroxyl endcapped macromer, a mercapto endcapped macromer or an amino endcapped macromer;
or D¹, X³and X⁴in formula (IIc) are selected so that HX³-D¹-X⁴H defines a hydroxyl endcapped macromer, a mercapto endcapped macromer or an amino endcapped macromer.

In another aspect the present invention provides a medical device that comprises a polymer and/or polymer composition as described herein. For example, an embodiment provides a stent that comprises a polymer composition as described herein. Another embodiment provides a method of treating a body lumen, comprising deploying the stent within the body lumen. These and other embodiments are described in greater detail below.

DETAILED DESCRIPTION OF THE INVENTION

The present invention relates to new classes of monomers useful for preparation of biocompatible polymers and phase-separated polymeric materials prepared by using these monomers. These phase-separated polymeric materials, while not limited thereto, are fracture- and/or fatigue-toughened, may be adapted for radio-opacity and are useful for medical device applications and controlled release therapeutic formulations, although not limited thereto.

ABBREVIATIONS AND NOMENCLATURE

As used herein, the terms “macromer”, “macromeric” and similar terms have the usual meaning known to those skilled in the art and thus may be used to refer to oligomeric and polymeric materials that are functionalized with end groups that are selected so that the macromers can be copolymerized with other monomers. A wide variety of macromers and methods for making them are known to those skilled in the art. Examples of suitable macromers include hydroxy endcapped polylactic acid macromers, hydroxy endcapped polyglycolic acid macromers, hydroxy endcapped poly(lactic acid-co-glycolic acid) macromers, hydroxy endcapped polycaprolactone macromers, poly(alkylene diol) macromers, hydroxy end-capped poly(alkylene oxide) macromers and hydroxy endcapped polydioxanone macromers.

As used herein, the terms “tough”, “toughened”, “toughness” and similar terms have the usual meaning known to those skilled in the art and thus may be used to refer to the resistance of a polymer under a static or dynamic load (or strain) to brittle failure from crack propagation within a glassy or semicrystalline phase. Examples of toughened polymers include fracture-toughened polymers and fatigue-toughened polymers. Preferred toughened polymer compositions contain multiple phases, including a toughening phase (such as an elastomeric phase) that is present in an amount that is effective to toughen the polymer composition, as compared to an otherwise comparable polymer composition lacking the toughening phase. The toughening phase may be a discrete phase that is not covalently attached to the other phase(s), or the polymer composition may include a block copolymer that phase separates in such a way as to form a toughening phase.

As used herein, the terms “polymer”, “polymeric” and similar terms have the usual meaning known to those skilled in the art and thus may be used to refer to homopolymers, copolymers (e.g., random copolymer, alternating copolymer, block copolymer, graft copolymer) and mixtures thereof.

As used herein, the term “molecular weight” has the usual meaning known to those skilled in the art and thus reference herein to a polymer having a particular molecular weight will be understood as a reference to a polymer molecular weight in units of Daltons. Various techniques known to those skilled in the art, such as end group analysis (e.g., by ¹H NMR) and high pressure size exclusion chromatography (also known as gel permeation chromatography, “GPC”), may be used to determine polymer molecular weights. In some cases the molecular weights of polymers are further described herein using the terms “number average” molecular weight (Mn) and/or “weight average” molecular weight (Mw), both of which terms are likewise expressed in units of Daltons and have the usual meaning known to those skilled in the art. In the event of that the value for the molecular weight of a polymer as determined by a particular technique conflicts with the value obtained by a different technique, the following procedure is used to resolve the conflict. An attempt is made to measure the number average molecular weight of the polymer by end group analysis using ¹H NMR. If ¹H NMR indicates that the number average molecular weight is greater than 10,000, then the molecular weight of the polymer is determined by GPC using low angle laser light scattering (LALLS) detection. On the other hand, if ¹H NMR indicates that the number average molecular weight is 10,000 or less, then the molecular weight of the polymer is determined by end group analysis using ¹H NMR.

As used herein, the terms “phase separated,” “phase separation” and similar terms have the usual meaning known to those skilled in the art and thus may be used to refer to polymeric materials that contain multiple phases, e.g., a polymeric material that contains a glassy phase and an elastomeric phase, a polymeric material that contains a crystalline phase and an elastomeric phase, a polymeric material that contains a semi-crystalline phase and an elastomeric phase, a polymeric material that contains a glassy phase, a semi-crystalline phase and an elastomeric phase, etc. The presence of phase separation may determined by one or more of a number of accepted methodologies, e.g., small angle neutron scattering (SANS), small angle x-ray scattering (SAXS), transmission electron microscopy (TEM), atomic force microscopy (AFM), and/or by the detection of multiple phase transitions attributable to each of the phases by differential scanning calorimetry (DSC), dynamic mechanical analysis (DMA), dielectric analysis (DEA), and/or thermal mechanical analysis (TMA). In the event that the results obtained by any two or more techniques conflict with one another, phase separation is to be determined by SAXS. Those skilled in the art will appreciate that phase separation can occur on various scales, e.g., microphase separation, nanophase separation, etc., and that phase separation may occur between two different polymers or between two phases of the same polymer. For example, some embodiments are directed to phase-separated block copolymers. In some embodiments a polymer composition is said to be phase-separated over a particular temperature range, e.g., over at least the temperature range of about 25° C. to about 50° C. This means that the polymer composition remains phase separated over the indicated range of temperatures. Phase separation over the entire range may be determined by confirming phase separation at three representative temperatures within the range, e.g., at the upper end of the range (e.g., about 50° C.), at the lower end of the range (e.g., about 25° C.) and in the middle of the range (e.g., about 37° C.).

The term “volume fraction” has the usual meaning known to those skilled in the art and thus may be used to refer to the respective amounts of two or more polymer components within a polymer composition, e.g., two or more phases. In many cases volume fraction is difficult to determine experimentally and thus for the purposes of this patent application the volume fraction is a calculated value based on the respective densities and masses of the individual polymer components. The calculation is not adjusted for phase mixing (if any) and is not adjusted for differences in density (if any) between amorphous and crystalline regions within a particular material. For example, for a three-component polymer composition, the total volume of the polymer composition, V_P, is defined as the sum of the volumes of the three components in the polymer composition, V₁+V₂+V₃, as follows:
V _P =V ₁ +V ₂ +V ₃

The volumes of the three components are defined in terms of their individual masses and densities, as follows:

- V₁=M₁/D₂, where M₁and D₁are the mass and density of component 1, respectively.
- V₂=M₂/D₂, where M₂and D₂are the mass and density of component 2, respectively.
- V₃=M₃/D₃, where M₃and D₃are the mass and density of component 3, respectively.

The volume fraction of each component is thus calculated as the volume of that component divided by the total volume:
Volume fraction of first component=V ₁ /V _P.
Volume fraction of second component=V ₂ /V _P.
Volume fraction of third component=V ₃ /V _P.

The masses (e.g., M₁, M₂and M₃) are determined from knowledge of the formulation for the particular polymer composition being made, and the densities (e.g., D₁, D₂and D₃) are based on the densities of each of the individual component, as measured in isolation.

The volume fraction of the various phases in a phase-separated composition is determined similarly. For the purposes of the volume fraction calculation, a component of the Formula (I-2) is considered to be phase-separated from a component that is not of the Formula (I-2) if the component that is not of the Formula (I-2) has a molecular weight of greater than 5,000. If two components of the Formula (I-2) have essentially the same chemical composition but different molecular weights, then both are considered to be in the same (first) phase. If two components that are not of the Formula (I-2) (e.g., two components of the formula (II)) have essentially the same chemical composition but different molecular weights, they are both considered to be in the same (second) phase if both of their molecular weights are greater than 5,000. However, if a component is not of the Formula (I-2) and has a molecular weight of 5,000 or less, it is considered to be in the first phase.

The term “thermal transition temperature” has the usual meaning known to those skilled in the art and thus may be used to refer to both first order thermal transitions and second order thermal transitions. The first order thermal transition of a polymer or phase thereof may be referred to herein as a “melting point” or “Tm”, and the second order thermal transition of a polymer or phase thereof may be referred to herein as a “glass transition temperature” or “Tg”. Those skilled in the art will appreciate that a polymeric material or phase thereof may have exhibit either or both types of thermal transitions, as well as higher order thermal transitions. Thermal transition temperature may be determined by methods known to those skilled in the art, such as by DSC, DMA, DEA and TMA. In some cases the presence of a particular phase within a polymer composition may be detectable by a technique such as SAXS, but the amount of that phase in the polymer composition may be relatively small, such that measurements of the thermal transition temperature(s) for that phase may be difficult or imprecise. In such instances, the thermal transitions temperatures may be determined by applying the measurement technique (e.g., DSC, DMA, DEA and/or TMA) to a bulk sample of a polymer composed of the recurring units present in that phase.

The term “wet” thermal transition temperature, e.g., “wet” melting point and “wet” glass transition temperature, refers to a thermal transition temperature of a polymer or phase thereof that is determined using a sample of the polymer that has been pre-conditioned to be in a wet state during the measurement by soaking the polymer for 24 hours at 37° C. in 0.1 M phosphate buffered saline (PBS) at pH 7.4. Wet thermal transition temperatures may be measured using various techniques known to those skilled in the art. In the event that the results obtained by any two or more techniques conflict with one another, DMA is used to determine wet thermal transition temperatures. DMA monitors changes in the viscoelastic properties (storage modulus, loss modulus, and tan delta) of a material as a function of temperature under oscillating deformation (stress or strain). The terms “stress at yield (a),” “modulus (E)”, and “elongation at break (∈)” have the usual meanings known to those skilled in the art. Although in principle all three viscoelastic properties can be used to define the Tg, for the present purposes Tg and/or Tm are determined by measuring the onset of drop in storage modulus E′, as indicated by the intersection of the respective tangent lines before and after the transition. Wet Tg and/or Tm can be determined using DMA by employing a submersion clamp apparatus that allows a film strip sample to be tested within a liquid environment. A thin (0.1 mm) strip of thermo-pressed polymer film is submerged in water and subjected to a multi-frequency-strain mode and heated at a constant rate. While heating, the material is deformed (oscillated) at constant amplitude (strain) over a range of frequencies (or single frequency) and mechanical properties measured, using the following DMA instrument parameters: temperature ramp: heat at rate of 2° C./min over temperature range of 5° C. to 80° C.; oscillating amplitude 20 μm; pre-load=0.01 N; frequency=1.2 Hz.

Because the sample is wet, the appearance of the E′ onset parameter can only be determined within the range of about 5° C. to about 80° C. because of the physical limitations due to the freezing and vaporization of water. If a wet transition is not found within the 5° C. to 80° C. range, then another analysis is performed in the dry state over a much greater temperature range, e.g., −150° C. to 200° C., and a new Tg analysis is made. With the dry Tg information, in combination with the lack of a wet transition, the wet Tg is thereby determined to be either below 5° C. or above 80° C., as the case may be.

The terms “radiopaque”, “radio-opaque”, “radiopacity”, “radio-opacity”, “radiopacifying” and similar terms have the usual meaning known to those skilled in the art and thus may be used to refer to polymer compositions that have been rendered easier to detect using medical imaging techniques (e.g., by X-ray and/or during fluoroscopy) being the incorporation of heavy atoms into the polymer composition. Such incorporation may be by mixing, e.g., by mixing an effective amount of a radiopacifying additive such as barium salt or complex, and/or by attachment of effective amounts of heavy atoms to one or more of the polymers in the polymer composition. For example, attachment of heavy atoms to a polymer in sufficient amounts may advantageously render the polymer easier to detect by various medical imaging techniques. The term “heavy atom” is used herein to refer to atoms having an atomic number of 17 or greater. Preferred heavy atoms have an atomic number of 35 or greater, and include bromine, iodine, bismuth, gold, platinum tantalum, tungsten, and barium. In certain configurations, polymer compositions may be inherently radiopaque. The term “inherently radiopaque” is used herein to refer to a polymer to which a sufficient number of heavy atoms are attached by covalent or ionic bonds to render the polymer radiopaque. This meaning is consistent with the understanding of those skilled in the art, see, e.g., U.S. Patent Publication No. 2006/0024266, which is hereby incorporated by reference for all purposes, including for the particular purpose of describing radiopaque polymeric materials.

The terms “alkyl”, “alkylene” and similar terms have the usual meaning known to those skilled in the art and thus may be used to refer to straight or branched hydrocarbon chain fully saturated (no double or triple bonds) hydrocarbon group. Terminal alkyl groups, e.g., of the general formula —C_nH_2n+1, may be referred to herein as “alkyl” groups, whereas linking alkyl groups, e.g., of the general formula —(CH₂)_n—, may be referred to herein as “alkylene” groups. The alkyl group may have 1 to 50 carbon atoms (whenever it appears herein, a numerical range such as “1 to 50” refers to each integer in the given range; e.g., “1 to 50 carbon atoms” means that the alkyl group may consist of 1 carbon atom, 2 carbon atoms, 3 carbon atoms, etc., up to and including 50 carbon atoms, although the present definition also covers the occurrence of the term “alkyl” where no numerical range is designated). The alkyl group may also be a medium size alkyl having 1 to 30 carbon atoms. The alkyl group could also be a lower alkyl having 1 to 5 carbon atoms. The alkyl group of the compounds may be designated as “C₁-C₄alkyl” or similar designations. By way of example only, “C₁-C₄alkyl” indicates that there are one to four carbon atoms in the alkyl chain, i.e., the alkyl chain is selected from the group consisting of methyl, ethyl, propyl, iso-propyl, n-butyl, iso-butyl, sec-butyl, and t-butyl. Typical alkyl groups include, but are in no way limited to, methyl, ethyl, propyl, isopropyl, butyl, isobutyl, tertiary butyl, pentyl, hexyl and the like.

The alkyl group may be substituted or unsubstituted. When substituted, the substituent group(s) is (are) one or more group(s) individually and independently selected from alkenyl, alkynyl, cycloalkyl, cycloalkenyl, cycloalkynyl, aryl, heteroaryl, heteroalicyclyl, aralkyl, heteroaralkyl, (heteroalicyclyl)alkyl, hydroxy, protected hydroxyl, alkoxy, aryloxy, acyl, ester, mercapto, cyano, halogen, carbonyl, thiocarbonyl, O-carbamyl, N-carbamyl, O-thiocarbamyl, N-thiocarbamyl, C-amido, N-amido, S-sulfonamido, N-sulfonamido, C-carboxy, protected C-carboxy, O-carboxy, isocyanato, thiocyanato, isothiocyanato, nitro, silyl, sulfenyl, sulfinyl, sulfonyl, haloalkyl, haloalkoxy, trihalomethanesulfonyl, trihalo-methanesulfonamido, and amino, including mono- and di-substituted amino groups, and the protected derivatives thereof.

The terms “alkenyl”, “alkenylene” and similar terms have the usual meaning known to those skilled in the art and thus may be used to refer to an alkyl or alkylene group that contains in the straight or branched hydrocarbon chain one or more double bonds. An alkenyl group may be unsubstituted or substituted. When substituted, the substituent(s) may be selected from the same groups disclosed above with regard to alkyl group substitution unless otherwise indicated.

The terms “heteroalkyl”, “heteroalkylene,” and similar terms have the usual meaning known to those skilled in the art and thus may be used to refer to an alkyl group or alkylene group as described herein in which one or more of the carbons atoms in the backbone of alkyl group or alkylene group has been replaced by a heteroatom such as nitrogen, sulfur and/or oxygen. Likewise, the term “heteroalkenylene” may be used to refer to an alkenyl or alkenylene group in which one or more of the carbons atoms in the backbone of alkyl group or alkylene group has been replaced by a heteroatom such as nitrogen, sulfur and/or oxygen.

The term “aryl” has the usual meaning known to those skilled in the art and thus may be used to refer to a carbocyclic (all carbon) monocyclic or multicyclic aromatic ring system that has a fully delocalized pi-electron system. Examples of aryl groups include, but are not limited to, benzene, naphthalene and azulene. The ring of the aryl group may have 5 to 50 carbon atoms. The aryl group may be substituted or unsubstituted. When substituted, hydrogen atoms are replaced by substituent group(s) that is (are) one or more group(s) independently selected from alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkenyl, cycloalkynyl, aryl, heteroaryl, heteroalicyclyl, aralkyl, heteroaralkyl, (heteroalicyclyl)alkyl, hydroxy, protected hydroxy, alkoxy, aryloxy, acyl, ester, mercapto, cyano, halogen, carbonyl, thiocarbonyl, O-carbamyl, N-carbamyl, O-thiocarbamyl, N-thiocarbamyl, C-amido, N-amido, S-sulfonamido, N-sulfonamido, C-carboxy, protected C-carboxy, O-carboxy, isocyanato, thiocyanato, isothiocyanato, nitro, silyl, sulfenyl, sulfinyl, sulfonyl, haloalkyl, haloalkoxy, trihalomethanesulfonyl, trihalomethanesulfonamido, and amino, including mono- and di-substituted amino groups, and the protected derivatives thereof, unless the substituent groups are otherwise indicated. An aryl group substituted with alkyl may be referred to herein as “alkylaryl.”

The term “heteroaryl” has the usual meaning known to those skilled in the art and thus may be used to refer to a monocyclic or multicyclic aromatic ring system (a ring system with fully delocalized pi-electron system) that contain(s) one or more heteroatoms, that is, an element other than carbon, including but not limited to, nitrogen, oxygen and sulfur. The ring of the heteroaryl group may have 5 to 50 atoms. The heteroaryl group may be substituted or unsubstituted. Examples of heteroaryl rings include, but are not limited to, furan, furazan, thiophene, benzothiophene, phthalazine, pyrrole, oxazole, benzoxazole, 1,2,3-oxadiazole, 1,2,4-oxadiazole, thiazole, 1,2,3-thiadiazole, 1,2,4-thiadiazole, benzothiazole, imidazole, benzimidazole, indole, indazole, pyrazole, benzopyrazole, isoxazole, benzoisoxazole, isothiazole, triazole, benzotriazole, thiadiazole, tetrazole, pyridine, pyridazine, pyrimidine, pyrazine, purine, pteridine, quinoline, isoquinoline, quinazoline, quinoxaline, cinnoline, and triazine. A heteroaryl group may be substituted or unsubstituted. When substituted, hydrogen atoms are replaced by substituent group(s) that is (are) one or more group(s) independently selected from alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkenyl, cycloalkynyl, aryl, heteroaryl, heteroalicyclyl, aralkyl, heteroaralkyl, (heteroalicyclyl)alkyl, hydroxy, protected hydroxy, alkoxy, aryloxy, acyl, ester, mercapto, cyano, halogen, carbonyl, thiocarbonyl, O-carbamyl, N-carbamyl, O-thiocarbamyl, N-thiocarbamyl, C-amido, N-amido, S-sulfonamido, N-sulfon-amido, C-carboxy, protected C-carboxy, O-carboxy, isocyanato, thiocyanato, isothiocyanato, nitro, silyl, sulfenyl, sulfinyl, sulfonyl, haloalkyl, haloalkoxy, trihalomethanesulfonyl, trihalo-methanesulfonamido, and amino, including mono- and di-substituted amino groups, and the protected derivatives thereof.

The term “crystallizable” has the usual meaning known to those skilled in the art, see U.S. Patent Publication No. 20060024266, which is incorporated herein by reference for all purposes and particularly for the purpose of describing crystallizable groups. Polymers that contain crystallizable groups that are attached to the sides of the polymer, known as side chain crystallizable (SCC) polymers or “comb-like” polymers, are well-known, see N. A. Plate and V. P. Shibaev, J. Polymer Sci.: Macromol. Rev. 8:117-253 (1974), the disclosure of which is hereby incorporated by reference. In an embodiment, a polymer as described herein contains crystallizable side groups and thus may be regarded as a SCC polymer. It will be understood that the crystallizable side chains of SCC polymers are preferably selected to crystallize with one another to form crystalline regions and may comprise, for example, —(CH₂)_x— and/or —((CH₂)_y—O—)_x— groups. The side chains are preferably linear to facilitate crystallization. For SCC polymers that contain —(CH₂)_x— groups in the crystallizable side chain, x is preferably in the range of about 6 to about 30, more preferably in the range of about 20 to about 30. For SCC polymers that contain —((CH₂)_y—O—)_xgroups in the crystallizable side chain, x is preferably in the range of about 6 to about 30 and y is preferably in the range of about 1 to about 8. More preferably, x and y are selected so that the ((CH₂)_y—O—)_xgroups contain from about 6 to about 30 carbon atoms, even more preferably from about 20 to about 30 carbon atoms. The spacing between side chains and the length and type of side chain are preferably selected to provide the resulting SCC polymer with a desired melting point. As the spacing between side chains increases, the tendency for the side chains to be crystallizable tends to decrease. Likewise, as the flexibility of the side chains increases, the tendency for the side chains to be crystallizable tends to decrease. On the other hand, as the length of the side chains increases, the tendency for the side chains to be crystallizable tends to increase. In many cases, the length of the crystallizable side chain may be in the range of about two times to about ten times the average distance between crystallizable side chains of the SCC polymer.

Whenever a group is described as being “optionally substituted” that group may be unsubstituted or substituted with one or more of the indicated substituents. Likewise, when a group is described as being “unsubstituted or substituted” if substituted, the substituent may be selected from one or more the indicated substituents.

Unless otherwise indicated, when a substituent is deemed to be “optionally substituted,” or “substituted” it is meant that the substituent is a group that may be substituted with one or more group(s) individually and independently selected from alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkenyl, cycloalkynyl, aryl, heteroaryl, heteroalicyclyl, aralkyl, heteroaralkyl, (heteroalicyclyl)alkyl, hydroxy, protected hydroxy, alkoxy, aryloxy, acyl, ester, mercapto, cyano, halogen, carbonyl, thiocarbonyl, O-carbamyl, N-carbamyl, O-thiocarbamyl, N-thiocarbamyl, C-amido, N-amido, S-sulfonamido, N-sulfonamido, C-carboxy, protected C-carboxy, O-carboxy, isocyanato, thiocyanato, isothiocyanato, nitro, silyl, sulfenyl, sulfinyl, sulfonyl, haloalkyl, haloalkoxy, trihalomethanesulfonyl, trihalomethanesulfonamido, and amino, including mono- and di-substituted amino groups, and the protected derivatives thereof. Similarly, the term “optionally ring-halogenated” may be used to refer to a group that optionally contains one or more (e.g., one, two, three or four) halogen substituents on the aryl and/or heteroaryl ring. The protecting groups that may form the protective derivatives of the above substituents are known to those of skill in the art and may be found in references such as Greene and Wuts, Protective Groups in Organic Synthesis, 3^rdEd., John Wiley & Sons, New York, N.Y., 1999, which is hereby incorporated by reference in its entirety.

It is understood that, in any compound described herein having one or more chiral centers, if an absolute stereochemistry is not expressly indicated, then each center may independently be of R-configuration or S-configuration or a mixture thereof. Thus, the compounds provided herein may be enantiomerically pure or be stereoisomeric mixtures. In addition it is understood that, in any compound having one or more double bond(s) generating geometrical isomers that can be defined as E or Z each double bond may independently be E or Z a mixture thereof. Likewise, all tautomeric forms are also intended to be included.

The following abbreviations are used to identify various iodinated compounds. TE stands for tyrosine ethyl ester, DAT stands for desaminotyrosine and DTE for desaminotyrosyl tyrosine ethyl ester. PTE stands for hydroxy-phenoxy-1-oxoethyl tyrosine ethyl ester. The polymer obtained by phosgenation of DTE is denoted as poly(DTE carbonate). An “I” before the abbreviation shows mono-iodination (e.g. ITE stands for mono-iodinated TE) and an I₂before the abbreviation shows di-iodination (e.g. I₂DAT stands for di-iodinated DAT). In DTE, if the “I” is before D, it means the iodine is on DAT and if “I” is after D, it means the iodine is on the tyrosine ring (e.g. DI₂TE stands for DTE with 2 iodine atoms on the tyrosine ring). The following diagram illustrates this nomenclature further.

General Structure of Iodinated DTE Monomer

IDTE: X^1a═I, X^1b═H, X^2a═H, X^2b═H.

I₂DTE: X^1a═I, X^1b═I, X^2a═H, X^2b═H

DI₂TE: X^1a═H, X^1b═H, X^2a═I, X^2b═I

IDITE: X^1a═I, X^1b═H, X^2a═I, X^2b═H

For PTE, PTH, IPTE, I₂PTE, PI₂TE, etc., the DAT CH₂CH₂is replaced with OCH₂.

As used herein, the abbreviations for any protective groups, amino acids and other compounds are, unless indicated otherwise, in accord with their common usage, recognized abbreviations, or the IUPAC-IUP Commission on Biochemical Nomenclature (See, Biochem. 11:942-944 (1972)).

PREFERRED EMBODIMENTS

wherein:

Diphenol compounds according to the present invention include compounds in which R²is —O—CH²—C(═O)— or —NR³—CH₂—C(═O)—.

wherein:

i is an integer selected from 1 through 4;

y¹and y²are each independently selected from 0, 1, 2, 3 and 4;

X¹and X², at each occurrence, are independently bromine (Br) or iodine (I);

R³is an optionally substituted C_1-30alkyl; and

In one embodiment of this aspect, the present invention provides new monomers having a structure of Formula (I), wherein the —OH group on the left phenyl ring is at an o-position.

In another embodiment of this aspect, the present invention provides new monomers having a structure of Formula (I), wherein the —OH group on the left phenyl ring is at an m-position.

In another embodiment of this aspect, the present invention provides new monomers having a structure of Formula (I), wherein the —OH group on the left phenyl ring is at the p-position.

In another embodiment of this aspect, the present invention provides new monomers having a structure of Formula (I), wherein i is 1.

In another embodiment of this aspect, the present invention provides new monomers having a structure of Formula (I), wherein R³is hydrogen or C_1-6alkyl, and R⁶is H or C_1-18alkyl

In another embodiment of this aspect, the present invention provides new monomers having a structure of Formula (I), wherein R³is hydrogen or C_1-6alkyl, R⁶is H or C_1-18alkyl, and the —OH group on the left phenyl ring is at the p-position.

In another aspect the present invention provides biocompatible polymers comprising a repeating unit of formula (I-1):

wherein:

i is an integer selected from 1 through 4;

y¹and y²are each independently selected from 0, 1, 2, 3 and 4;

X¹and X², at each occurrence, are independently bromine (Br) or iodine (I);

X is oxygen (O), sulfur (S), or NR⁴, where R⁴is selected from the group consisting of

R³is an optionally substituted C_1-30alkyl; and

wherein the —X¹and —O— groups on the left phenyl ring, at each occurrence, are independently at o-, m-, or p-positions.

In one embodiment of this aspect, the present invention provides polymers comprising a repeating unit of Formula (I-1), wherein the —O— group on the left phenyl ring is at an o-position.

In another embodiment of this aspect, the present invention provides polymers comprising a repeating unit of Formula (I-1), wherein the —O— group on the left phenyl ring is at an m-position.

In another embodiment of this aspect, the present invention provides polymers comprising a repeating unit of Formula (I-1), wherein the —O— group on the left phenyl ring is at the p-position.

In another embodiment of this aspect, the present invention provides polymers comprising a repeating unit of Formula (I-1), wherein i is 1.

In another embodiment of this aspect, the present invention provides polymers comprising a repeating unit of Formula (I-1), wherein R³is hydrogen or C_1-6alkyl, and R⁶is H or C_1-18alkyl

In another embodiment of this aspect, the present invention provides polymers comprising a repeating unit of Formula (I-1), wherein R³is hydrogen or C_1-6alkyl, R⁶is H or C_1-18alkyl, and the —O— group on the left phenyl ring is at the p-position.

The monomer compounds are polymerized to form tissue compatible bioerodable polymers for medical uses. The diphenol monomers can be used in any conventional polymerization process using diphenol monomers, including those processes that synthesize polymers traditionally considered hydrolytically stable and non-biodegradable.

In this aspect the present invention provides biocompatible polymers comprising a repeating unit of formula (I-2):

wherein:

i is an integer selected from 1 through 4;

y¹and y²are each independently selected from 0, 1, 2, 3 and 4;

X¹and X², at each occurrence, are independently bromine (Br) or iodine (I);

R³is an optionally substituted C_1-30alkyl;

A¹is selected from:

In one embodiment of this aspect, the present invention provides polymers comprising a repeating unit of Formula (I-2), wherein the —O— group on the left phenyl ring is at an o-position.

In another embodiment of this aspect, the present invention provides polymers comprising a repeating unit of Formula (I-2), wherein the —O— group on the left phenyl ring is at an m-position.

In another embodiment of this aspect, the present invention provides polymers comprising a repeating unit of Formula (I-2), wherein the —O— group on the left phenyl ring is at the p-position.

In another embodiment of this aspect, the present invention provides polymers comprising a repeating unit of Formula (I-2), wherein i is 1.

In another embodiment of this aspect, the present invention provides polymers comprising a repeating unit of Formula (I-2), wherein R³is hydrogen or C_1-6alkyl, and R⁶is H or C_1-18alkyl

In another embodiment of this aspect, the present invention provides polymers comprising a repeating unit of Formula (I-2), wherein R³is hydrogen or C_1-6alkyl, R⁶is H or C_1-18alkyl, and the —O— group on the left phenyl ring is at the p-position.

In another embodiment of this aspect, the present invention provides polymers comprising a repeating unit of Formula (I-2), wherein R³is hydrogen or C_1-6alkyl, R⁶is H or C_1-18alkyl, and the —O— group on the left phenyl ring is at the p-position, and A¹is carbonyl —C(═O)—.

In this aspect the present invention provides a biocompatible polymer comprising a repeating unit derived from the monomer of Formula (I). The polymers include the above-depicted polyesters, polycarbonates, polyimino-carbonates, polyarylates, polyurethanes, polyphosphazine polyphosphonates and polyethers, as well as random block copolymers of these polymers with poly(alkylene oxides) as described in U.S. Pat. No. 5,658,995, the disclosure of which is incorporated herein by reference.

It is also understood that the presentation of the various polymer formulae that polymer structures represented may include homopolymers and heteropolymers, which include stereoisomers. Homopolymer is used herein to designate a polymer comprised of all the same type of monomers. Heteropolymer is used herein to designate a polymer comprised of two or more different types of monomer, which is also called a co-polymer. A heteropolymer or co-polymer may be of a kind known as block, random and alternating. Further with respect to the presentation of the various polymer formulae, products according to embodiments of the present invention may be comprised of a homopolymer, heteropolymer and/or a blend of such polymers.

Polyiminocarbonates are synthesized from diphenol monomers via one of the appropriate methods disclosed by U.S. Pat. No. 4,980,449, the disclosure of which is incorporated by reference. According to one method, part of the diphenol compound is converted to the appropriate dicyanate, then, equimolar quantities of the diphenol compound and the dicyanate are polymerized in the presence of a strong base catalyst such as a metal alkoxide or metal hydroxide.

The monomers compounds of Formula (I) may also be reacted with phosgene to form polycarbonates with —O—C(═O)—O— linkages. The method is essentially the conventional method for polymerizing diols into polycarbonates. Suitable processes, associated catalysts and solvents are known in the art and are taught in Schnell, Chemistry and Physics of Polycarbonates, (Interscience, New York 1964), the teachings of which are also incorporated herein by reference.

Other methods adaptable for use to prepare polycarbonate polymers of the present invention are disclosed in U.S. Pat. Nos. 6,120,491, and 6,475,477 the disclosures of which are incorporated herein by reference. Polycarbonates may also be prepared by dissolving the Formula I monomer in methylene chloride containing 0.1M pyridine or triethylamine. A solution of phosgene in toluene at a concentration between about 10 and about 25 wt %, and preferably about 20 wt %, is added at a constant rate, typically over about two hours, using a syringe pump or other means. The reaction mixture is quenched by stirring with tetrahydrofuran (THF) and water, after which the polymer is isolated by precipitation with isopropanol (IPA). Residual pyridine (if used) is then removed by agitation of a THF polymer solution with a strongly acidic resin, such as AMBERLYST 15.

The monomer compounds of Formula (I) may also be directly reacted with aliphatic or aromatic dicarboxylic acids in the carbodiimide mediated process disclosed by U.S. Pat. No. 5,216,115 using 4-(dimethylamino)pyridinium-p-toluene sulfonate (DPTS) as a catalyst to form the aliphatic or aromatic poly(ester amides). The disclosure of U.S. Pat. No. 5,216,115 is incorporated by reference. Dicarboxylic acids according to one embodiment of the present invention have the structure of Formula V:
HOOC—R₅—COOH (V)

in which, for the aliphatic copolymers, R₅is selected from saturated and unsaturated, substituted and unsubstituted alkyl groups containing up to 18 carbon atoms, and preferably from 2 to 12 carbon atoms, and optionally may also include up to eight N, O, P or S atoms. For the aromatic copolymers, R₃is selected from aryl and alkylaryl groups containing up to 24 carbon atoms and preferably from 13 to 20 carbon atoms, and optionally may also include up to eight N, O, P or S atoms. The N-heteroatoms may be N-substituted to reduce polymer T_gand melt viscosity.

The process forms polymers with —O—C(═O)—R₅—C(═O)—O— linkages. R₅may be selected so that the dicarboxylic acids employed as the starting materials are either important naturally-occurring metabolites or highly biocompatible compounds. Aliphatic dicarboxylic acid starting materials therefore include the intermediate dicarboxylic acids of the cellular respiration pathway known as the Krebs Cycle. The dicarboxylic acids include α-ketoglutaric acid, succinic acid, fumaric acid and oxaloacetic acid (R₅of Formula V is —CH₂—CH₂—C(═O)—, —CH₂—CH₂—, —CH═CH— and —CH₂—C(═O)—, respectively).

Another naturally-occurring aliphatic dicarboxylic acid is adipic acid (R₅is (—CH₂—)₄), found in beet juice. Still yet another biocompatible aliphatic dicarboxylic acid is sebacic acid (R₅is (—CH₂—)₈), which has been studied extensively and has been found to be nontoxic as part of the clinical evaluation of poly(bis(p-carboxy-phenoxy)propane-co-sebacic acid anhydride) by Laurencin et al., J. Biomed. Mater. Res., 24, 1463-81 (1990).

Other biocompatible aliphatic dicarboxylic acids include oxalic acid (R₅is a bond), malonic acid (R₅is —CH₂—), glutaric acid (R₅is (—CH₂—)₃), pimelic acid (R₅is (—CH₂—)₅), suberic acid (R₅is (—CH₂—)₆) and azelaic acid (R₅is (—CH₂—)₇). R₅can thus represent (—CH₂—)_Q, wherein Q is between 0 and 8, inclusive. Among the suitable aromatic dicarboxylic acids are terephthalic acid, isophthalic acid and bis(p-carboxy-phenoxy)alkanes such as bis(p-carboxy-phenoxy)propane.

R₅can also have the structure of Formula VI:
—(CH₂—)_aO—[(CH₂—)_aCHR₈—O—]_m(CH₂—)_a (VI)

wherein a is from 1 to 3, inclusive, m is from 1 to 500,000, inclusive, and R₄is hydrogen or a lower alkyl group containing from one to four carbon atoms. R₄is preferably hydrogen, a is preferably 1, and m is preferably between about 10 and about 100, and more preferably between about 10 and about 50.

The diacids of Formula VI are formed by the oxidation of poly(alkylene oxides) according to well-known methods. One example of such a compound is biscarboxymethyl poly(ethylene glycol), which is commercially available.

R₅can also have the structure of Formula VII:
—R₇—C(═O)—O[(—CH₂—)_a—CHR₈—O—]_mC(═O)—R₇ (VII)

wherein a, m and R₈and the preferred species thereof are the same as described above with respect to Formula VI. R₇is selected from a bond or straight and branched alkyl and alkylaryl groups containing up to 18 carbon atoms.

The dicarboxylic acids of Formula VII are poly(alkylene oxides)bis-functionalized with dicarboxylic acids having the structure of Formula V wherein R₅is the same as described above for Formula V and preferably contains up to 12 carbon atoms.

The poly(alkylene oxides) of Formula VII that are bis-functionalized with dicarboxylic acid are prepared by the reaction of a non-functionalized poly(alkylene oxide) with an excess of either the dicarboxylic acid (mediated by a coupling agent such as dicyclohexyl carbodiimide), the anhydride (e.g. succinic anhydride) in the presence of pyridine or triethylamine, or a dicarboxylic acid chloride (e.g. adipoyl chloride) in the presence of an acid acceptor like triethylamine.

Polymers prepared from the Formula I monomeric starting materials of the present invention with at least one bromine- or iodine-substituted aromatic ring are radio-opaque, such as the polymers prepared from radiopaque diphenol compounds prepared according to the disclosure of U.S. Pat. No. 6,475,477, as well as the disclosure of co-pending and commonly-owned U.S. patent application Ser. No. 10/592,202, the disclosures of both of which are incorporated herein by reference. The iodinated and brominated diphenol monomers of the present invention can also be employed as radio-opacifying, biocompatible non-toxic additives for other polymeric biomaterials.

Bromine and iodine substituted aromatic monomers of the present invention are prepared by well-known iodination and bromination techniques that can be readily employed by those of ordinary skill in the art guided by the above referenced granted patent and pending application (now published) without undue experimentation. The halogenated aromatic compounds from which the halogenated aromatic monomers the present invention are prepared undergo ortho-directed halogenation. The term, “ortho-directed”, is used herein to designate orientation of the halogen atom(s) relative to the phenoxy alcohol group.

Random or block copolymers of the Formula Ia polymers of the present invention with a poly(alkylene oxide) may be prepared according to the method disclosed in U.S. Pat. No. 5,658,995, the disclosure of which is also incorporated by reference. The poly(alkylene oxide) is preferably a poly(ethylene glycol) block/unit typically having a molecular weight of less than about 10,000 per unit. More typically, the poly(ethylene glycol) block/unit has a molecular weight less than about 4000 per unit. The molecular weight is preferably between about 1000 and about 2000 per unit.

The molar fraction of poly(ethylene glycol) units in block copolymers may range from greater than zero to less than 1, and is typically greater than zero up to about 0.5, inclusive. More preferably, the molar fraction is less than about 0.25 and yet more preferably, less than about 0.1. In a more preferred variations, the molar fraction may vary from greater than about 0.001 to about 0.08, and most preferably, between about 0.025 and about 0.035.

Unless otherwise indicated, the molar fractions reported herein are based on the total molar amount of poly(alkylene glycol) and non-glycol units in the polymers.

Thus, in another aspect the present invention provides a biocompatible polymer, comprising:

a first polymer recurring unit of Formula (I-2):

a second polymer recurring unit of Formula (III):

and/or
a third polymer recurring unit of Formula (IV):

wherein:

i is an integer selected from 1 through 4;

j is an integer in the range from 1 to about 500;

y¹and y²are each independently selected from 0, 1, 2, 3 and 4;

X¹and X², at each occurrence, are independently bromine (Br) or iodine (I);

X is oxygen (O), sulfur (S), or NR⁴; where R⁴is selected from the group consisting of hydrogen and alkyl containing from 1 to 6 carbon atoms

R³is an optionally substituted C_1-30alkyl;

A¹is selected from:

wherein the —X¹and —O— groups on the left phenyl ring of the first recurring unit, at each occurrence, are independently at o-, m-, or p-positions; and

wherein the amounts of unit (I-2), unit (III), and unit (IV) are in a:b:c ratio, wherein each of a, b, and c is a number between 0 to 1, inclusive, provided that a+b+c=1, and a is not equal to 0; and

wherein the repeating unit (I-2), unit (III), and unit (IV) appear in the polymer molecule alternately, in blocks, or randomly.

In one embodiment of this aspect, the present invention provides a polymer comprising repeating at least one repeating unit of Formula (I-2), at least one repeating unit of Formula (III), and at least one repeating units of Formula (IV).

In another embodiment of this aspect, the present invention provides a polymer comprising at least one repeating unit of Formula (I-2) and at least one repeating unit of Formula (III), in the absence of the repeating unit of Formula (IV).

In another embodiment of this aspect, the present invention provides a polymer comprising at least one repeating unit of Formula (I-2) and at least one repeating unit of Formula (IV), in the absence of the repeating unit of Formula (III).

In another embodiment of this aspect, the present invention provides a polymer comprising at least one repeating unit of Formula (I-2) and at least one repeating unit of Formula (IV) and/or Formula (III), wherein A¹is a carbonyl —C(═O)—.

Applicants have also recognized that the polymer glass transition temperature increases as the degree of halogenation and the molar fraction of free carboxylic acid units increases. Higher weight percentages of poly(alkylene oxide) are typically used in polymers with higher levels of iodination and/or with higher molar fractions of free carboxylic acid units to maintain the polymer glass transition temperature within a desired range for the end use application. N-alkylation provides an alternative means for lowering the polymer glass transition temperature so that the amount of poly(alkylene oxide) may be lowered or eliminated without adversely affecting the polymer melt properties. The present invention thus places more tools at the disposal of the polymer chemist for fine-tuning the physico-mechanical properties of the inventive polymers.

The Formula Ia polymers having weight-average molecular weights above about 20,000, and preferably above about 80,000, calculated from gel permeation chromatography (GPC) relative to polystyrene standards using tetrahydrofuran (THF) as the eluent without further correction.

The polymers of the present invention are defined as including polymers polymerized from Formula I monomers having pendent free carboxylic acid groups (R₆═H). However, it is not possible to polymerize polymers having pendent free carboxylic acid groups from corresponding monomers with pendent free carboxylic acid groups without cross-reaction of the free carboxylic acid group with the co-monomer. Accordingly, polymers in accordance with the present invention having pendent free carboxylic acid groups are prepared from homopolymers and copolymers of benzyl and tert-butyl ester monomers of the present invention having the structure of Formula IV in which R₈is a benzyl or tert-butyl group.

The benzyl ester homopolymers and copolymers may be converted to corresponding free carboxylic acid homopolymers and copolymers through the selective removal of the benzyl groups by the palladium catalyzed hydrogenolysis method disclosed by co-pending and commonly owned U.S. Pat. No. 6,120,491, the disclosure of which is incorporated herein by reference.

The tert-butyl ester homopolymers and copolymers may be converted to corresponding free carboxylic acid homopolymers and copolymers through the selective removal of the tert-butyl groups by the acidolyis method disclosed by the above-referenced U.S. patent application Ser. No. 10/592,202, also incorporated herein by reference.

The catalytic hydrogenolysis or acidolysis is necessary because the lability of the polymer backbone prevents the employment of harsher hydrolysis techniques.

Applicants have recognized that the molar fraction of free carboxylic acid units in the polymers of the present invention can be adjusted according to the present invention to likewise adjust the degradation/resorbability of devices made from such polymers. For example, applicants have recognized that while poly(DTE-co-35 mol % DT carbonate), (a tyrosine-derived polycarbonate comprising about 35% free carboxylic acid units) is 90% resorbed in about 15 days, polycarbonates with lower amounts of free carboxylic acid will have desirably longer lifetimes in the body. Furthermore, by otherwise adjusting the amount of free carboxylic acid in the polymers across the range of preferred molar fraction, the resulting polymers can be adapted for use in various applications requiring different device lifetimes. In general, the higher the molar fraction of free carboxylic acid units, the shorter the lifetime of the device in the body and more suitable such devices are for applications wherein shorter lifetimes are required. In certain embodiments where lifetimes of 6 months or more are required, polymers of the presently preferred ranges of free carboxylic acid units tend to be desirable.

After polymerization, appropriate work up of the polymers in accordance with preferred embodiments of the present invention may be achieved by any of a variety of known methods commonly employed in the field of synthetic polymers to produce a variety of useful articles with valuable physical and chemical properties, all derived from tissue compatible monomers. The useful articles can be shaped by conventional polymer-forming techniques such as extrusion, compression molding, injection molding, solvent casting, spin casting, wet spinning, combinations of two or more thereof, and the like. Shaped articles prepared from the polymers are useful, inter alia, as degradable biomaterials for medical implant applications. Such applications include the use of shaped articles as vascular grafts and stents.

Polymers according to the present invention also include polyethers, polyurethanes, poly(carbamates), poly(thiocarbonates), poly(carbonodithionates) and poly(thiocarbamates), which may be prepared from the diphenol compounds of the present invention in accordance with known methods.

wherein:

i is an integer selected from 1 through 4;

y¹and y²are each independently selected from 0, 1, 2, 3 and 4;

X¹and X², at each occurrence, are independently bromine (Br) or iodine (I);

X is oxygen (O), sulfur (S), or NR⁴;

R³is an optionally substituted C_1-30alkyl;

A¹is selected from:

a number (n) of first polymer recurring unit of Formula (I-2):

wherein:

i is an integer selected from 1 through 4;

y¹and y²are each independently selected from 0, 1, 2, 3 and 4;

X¹and X², at each occurrence, are independently bromine (Br) or iodine (I);

R³is an optionally substituted C_1-30alkyl;

A¹is selected from:

In these two aspects, the present invention provides a biocompatible polymer composition, comprising at least a first polymer phase and a second polymer phase. In some embodiments the biocompatible polymer composition is bioresorbable, biodegradable, or both. In some embodiments the polymer composition is radiopaque, whereas in other embodiments it is not radiopaque. Some radiopaque polymer compositions comprise a radiopacifying agent in an amount effective to render the polymer composition radiopaque. Other polymer compositions are inherently radiopaque, e.g., contain sufficient halogen atoms attached to one or more of the polymers in the composition to render the composition inherently radiopaque. In some embodiments, the polymer composition is rendered radiopaque by the combination of halogenation of one or more of the polymer constituents and by the inclusion of a radiopacifying agent. In some embodiments the polymer compositions comprises a biologically active compound (e.g., a drug), which may be dispersed in the polymer composition and/or covalently attached to the first polymer phase, the second polymer phase or both. Various aspects of the aforementioned polymer compositions are described in greater detail below.

The first polymer phase of the biocompatible polymer composition has at least one first wet thermal transition temperature selected from a first wet glass transition temperature and a first wet melting point, where the first wet thermal transition temperature is at least about 38° C. In other embodiments, the first wet thermal transition temperature is at least about 40° C., at least about 45° C., or at least about 50° C. In some embodiments the first polymer phase is crystalline, in other embodiments it is semi-crystalline, and in other embodiments it is glassy. For example, in some embodiments the first polymer phase is at least partially crystalline at a temperature below 37° C., e.g., by selecting the first recurring units of the Formula (I-2) such that the first polymer phase contains sufficient crystallizable side chains to render the first polymer phase at least partially crystalline at a temperature below 37° C.

The first polymer phase comprises a number (n) of first recurring units of the formula (Ia) as set forth above. In Formula (I-2), X¹and X²are each independently halogen, preferably bromine (Br) or iodine (I). The variables y¹and y²indicate the number of X¹and X²groups, respectively, and are each independently zero or an integer in the range of 1 to 4. In some embodiments, the first recurring units of the Formula (I-2) are selected to contain sufficient heavy atomes (e.g., halogen atoms) to render the polymer composition inherently radiopaque. For example, routine experimentation may be used to selected X¹, X², y¹and/or y²so as to render the resulting polymeric material radiopaque.

Each A¹in Formula (I-2) is independently selected from the group consisting of

where each R³is independently selected from the group consisting of C₁-C₃₀alkyl, C₁-C₃₀heteroalkyl, C₅-C₃₀aryl, C₆-C₃₀alkylaryl, and C₂-C₃₀heteroaryl, and each R⁴independently selected from the group consisting of H, C₁-C₃₀alkyl, and C₁-C₃₀heteroalkyl.

In some embodiments, A¹is a carbonyl linkage:

The second polymer phase of the biocompatible polymer composition has at least one second wet thermal transition temperature selected from a second wet glass transition temperature and a second wet melting point. The second wet thermal transition temperature is 36° C. or lower. In some embodiments the second wet thermal transition temperature is 25° C. or lower, 20° C. or lower, or 5° C. or lower. In some embodiments the second polymer phase is crystalline, in other embodiments it is semi-crystalline, and in other embodiments it is glassy. Various second recurring units are described in greater detail below.

The first polymer phase comprises a number (n) of first recurring units of the Formula (I-2) as set forth above, and the second polymer phase comprises a number (m) of second recurring units. The number (n) and the number (m) are selected to control the relative amounts of the first polymer phase and the second polymer phase so that the polymer composition is phase-separated over at least the temperature range of about 25° C. to about 50° C., and preferably over the temperature range of about 10° C. to about 70° C.

Those skilled in the art will understand that the various first recurring units of the Formula (I-2) need not be identical to one another, and thus the number (n) of first recurring units of the Formula (I-2) may include two or more recurring units of the Formula (I-2) that differ from one another in chemical structure. Likewise, the various second recurring units in the polymer composition need not be identical to one another, and thus the number (m) of second recurring units may include two or more recurring units that differ from one another in chemical structure. For example, in an embodiment, a polymer composition comprises second recurring units having a formula selected from the group consisting of the formula (IIa), the formula (IIb), the formula (IIc), and the formula (IId):

wherein X³, X⁴, X⁵, X⁷, X⁸, X⁹, X¹⁰, X¹¹, X¹²and X¹³are each independently selected from the group consisting of O, S and NR¹⁰, where R¹⁰is selected from hydrogen and an alkyl group containing from one to 30 carbon atoms;
Ar¹and Ar²are phenyl rings optionally substituted with from one to four substituents independently selected from the group consisting of a halogen, a halomethyl, a halomethoxy, a methyl, a methoxy, a thiomethyl, a nitro, a sulfoxide, and a sulfonyl;
R⁸and R⁹contain from one to ten carbon atoms each and are independently selected from the group consisting of an optionally substituted alkylene, an optionally substituted heteroalkylene, an optionally substituted alkenylene, and an optionally substituted heteroalkenylene;
g and h in Formula (IId) are each independently integers in the range of about 1 to about 500; and
D and D¹contain up to 24 carbon atoms and are independently selected from the group consisting of an optionally substituted alkylene, an optionally substituted heteroalkylene, an optionally substituted alkenylene and an optionally substituted heteroalkenylene;
or D, X⁸and X⁹in Formula (IIa) are selected so that HX⁸-D-X⁹H defines a hydroxyl endcapped macromer, a mercapto endcapped macromer or an amino endcapped macromer;
or D¹, X³and X⁴in Formula (IIc) are selected so that HX³-D¹-X⁴H defines a hydroxyl endcapped macromer, a mercapto endcapped macromer or an amino endcapped macromer.

Heteroalkylenes include oxyalkylenes, aminoalkylenes and thioalkylenes in which an oxygen, nitrogen or sulfur is substituted on a phenyl ring. Heteroalkenylenes include oxyalkenylenes, aminoalkenylenes and thioalkenylenes in which an oxygen, nitrogen or sulfur is substituted on a phenyl ring.

Such a polymer may optionally further comprise third recurring units having a formula that is also selected from the group consisting of the formula (IIa), the formula (IIb), the formula (IIc), and the formula (IId), wherein the third recurring units differ from the second recurring units.

In some embodiments the first polymer phase is covalently attached to the second polymer phase. For example, in an embodiment the polymer composition comprises a block copolymer that includes at least a first block and a second block, wherein the block copolymer is phase-separated so that more than about half of the first block is in the first polymer phase and more than about half of the second block is in the second polymer phase. In some embodiments the first polymer phase is not covalently attached to the second polymer phase. For example, in an embodiment, the first polymer phase comprises a first polymer and the second polymer phase comprises a second polymer that is different from the first polymer.

In an embodiment, the number (n) and the number (m) are selected to control the relative amounts of the first polymer phase and the second polymer phase so that the polymer composition has a water content of 4.5% or less, preferably 3.0% or less, more preferably 2.0% or less, as measured by Karl Fisher analysis after soaking for 24 hours at 37° C. in 0.1 M phosphate buffered saline (PBS) at pH 7.4.

In an embodiment, the number (n) and the number (m) are selected to control the relative amounts of the first polymer phase and the second polymer phase so that the volume fraction of the second polymer phase in the polymer composition is in the range of about 6% to about 40%, e.g., about 7% to about 33%, about 7% to about 26%, about 7% to about 20%, about 7% to about 26%, about 7% to about 16%, about 9% to about 16%, about 9% to about 13%, about 10% to about 13%, or about 10% to about 12%, based on total volume.

In an embodiment, the number (n) and the number (m) are selected to control the relative amounts of the first polymer phase and the second polymer phase so that the weight fraction of the second polymer phase in the polymer composition is in the range of about 6% to about 20%, preferably about 8% to about 18%, preferably about 10% to about 15%, based on total weight.

In an embodiment, the number (n) and the number (m) are selected to control the ductility properties of the polymer composition, such that the polymer composition has an elongation at break of greater than 30%, preferably greater than 50%, more preferably greater than 70%; a Young's modulus of greater than 130 ksi, preferably greater than 180 ksi; and/or a strength at yield of greater than 4.0 ksi, preferably greater than 6.4 ksi. The aforementioned ductility properties are measured when the polymer composition is tested under tensile test conditions that comprise (i) providing a tensile test strip having measurements of 0.2 inches wide, a gauge length of 1.0 inches and a thickness of 0.004 inches, (ii) aging the tensile test strip by soaking it for 7 days at 37° C. in 0.1 M phosphate buffered saline (PBS) at pH 7.4 to provide an aged tensile test strip, and (iii) pulling the aged tensile test strip at a rate of 10 inches per minute while submerged in water at 37° C. Multiple tensile test strips (e.g., 3-5) are tested, and the results averaged. A more detailed description of suitable tensile test conditions is described in the working examples below.

In an embodiment, wherein the number (n) and the number (m) are selected to control the fatigue properties of the polymer composition, such that the polymer composition remains intact for at least 60 minutes, preferably at least 80 minutes, more preferably at least 100 minutes. The aforementioned fatigue properties are measured when the polymer composition is tested under fatigue test conditions that comprise (i) providing a fatigue test strip having measurements of 5.0 mm wide, a gauge length of 15 mm and a thickness of 0.1 mm, (ii) aging the fatigue test strip by soaking it for 7 days at 37° C. in 0.1 M phosphate buffered saline (PBS) at pH 7.4 to provide an aged fatigue test strip, and (iii) subjecting the aged fatigue test strip to oscillating deformation at a frequency of 1.2 Hz under a stress of 10 MPa in single frequency stress mode while submerged in water at 37° C. Multiple fatigue test strips (e.g., 3-5) are tested, and the results averaged. A more detailed description of suitable fatigue test conditions is described in the working examples below.

The second polymer phase in the polymer composition may include various second recurring units. For example, in an embodiment, the second recurring units have a formula selected from the group consisting of the formula (IIa), the formula (IIb), the formula (IIc), and the formula (IId), which may be referred to collectively herein as being of the formula (II).

In formula (II), the variables X³, X⁴, X⁵, X⁷, X⁸, X⁹, X¹⁰, X¹¹, X¹²and X¹³are each independently selected from the group consisting of O, S and NR¹⁰, where R¹⁰is selected from hydrogen and an alkyl group containing from one to 30 carbon atoms. In various embodiment, all of the variables X³, X⁴, X⁵, X⁷, X⁸, X⁹, X¹⁰, X¹¹, X¹²and X¹³are O.

In the formula (IId), the variables Ar¹and Ar²are phenyl rings optionally substituted with from one to four substituents independently selected from the group consisting of a halogen, a halomethyl, a halomethoxy, a methyl, a methoxy, a thiomethyl, a nitro, a sulfoxide, and a sulfonyl. In an embodiment, the Ar¹and/or Ar²phenyl rings are substituted with a halogen and/or halogen-containing substituent such as halomethyl and/or halo-methoxy. In an embodiment, the Ar¹and/or Ar²phenyl rings are substituted with a number of halogen and/or halogen-containing substituents that is effective to render the resulting polymer composition radiopaque. Preferred halogens for rendering polymers radiopaque include bromine and iodine.

In the formula (II), the variables R⁸and R⁹contain from one to ten carbon atoms each and are independently selected from the group consisting of an optionally substituted alkylene, an optionally substituted heteroalkylene, an optionally substituted alkenylene, and an optionally substituted heteroalkenylene.

The variables g and h in formula (IId) are each independently integers in the range of about 1 to about 500, preferably in the range of about 5 to about 100. Thus, the sum of g+h may be in the range of about 2 to about 1000. In various embodiments, the sum of g+h is greater than about 35, preferably greater than about 40, even more preferably greater than about 50. In various embodiments, the sum of g+h in the range of about 18 to about 26; from about 32 to about 39; from about 36 to about 52; from about 79 to about 96; and from about 170 to 200.

In various embodiments, the recurring units of the formula (IId) result from copolymerizing a hydroxy endcapped polycaprolactone (PCL) macromer of the following formula:

In various embodiments, D¹in formula (IId), and in the hydroxy endcapped poly-caprolactone (PCL) macromer depicted, above is C₁-C₂₄alkylene, e.g., —(CH₂)_t, where t is 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10. In various embodiments, g and h are selected for the recurring units of the formula (IId) so that the hydroxy endcapped polycaprolactone (PCL) macromer depicted above has a molecular weight in the range of about 2,000 to about 40,000, e.g., in ranges of about 2,500 to about 3,500; about 4,000 to about 6,000; about 7,000 to about 9,000; about 8,000 to about 12,000; about 18,000 to about 22,000; or about 38,000 to about 46,000.

Embodiments of polymers described herein may be prepared in various ways, e.g., as taught expressly herein or by adapting methods known to those skilled in the art in view of the guidance provided herein. For example, block copolymers may be prepared by reacting a first monomer of the Formula (I):

with a second monomer of the formula
HX⁸-D-X⁹—(C═X⁹)—X⁹H, the formula HX³-D¹-(C═X⁴)—X⁴H, the formula

and/or the formula

wherein the variables in the above second monomer structures are defined in the same manner as the corresponding polymers described herein. As described above, D, X⁸and X⁹may be selected so that HX⁸-D-X⁹H defines a hydroxyl endcapped macromer, a mercapto endcapped macromer or an amino endcapped macromer. Likewise, D¹, X³and X⁴may be selected so that HX³-D¹-X⁴H defines a hydroxyl endcapped macromer, a mercapto endcapped macromer or an amino endcapped macromer. For example, HX³-D¹-X⁴H and HX⁸-D-X⁹H may each independently represent a macromer selected from a hydroxy endcapped polylactic acid macromer, a hydroxy endcapped polyglycolic acid macromer, a hydroxy endcapped poly(lactic acid-co-glycolic acid) macromer, a hydroxy endcapped poly-caprolactone macromer, a poly(alkylene diol) macromer, a hydroxy end-capped poly(alkylene oxide) macromer and a hydroxy endcapped polydioxanone macromer.

Those skilled in the art will recognize that by selecting a monomer of formula (Ia) having the appropriate variable groups, the first recurring units of the resulting polymer are tyrosine-derived diphenol repeating units. The diphenol monomers may be prepared by means of carbodiimide-mediated coupling reactions in the presence of hydroxybenzotriazole according to the procedure disclosed in U.S. Pat. Nos. 5,587,507 and 5,670,602, the disclosures of both of which are hereby incorporated by reference. Suitable carbodiimides are disclosed therein. The preferred carbodiimide is 1-ethyl-3-(3-dimethylamino-propyl)carbodiimide hydrochloride (EDCI.HCl). The crude monomers can be recrystallized twice, first from 50% acetic acid and water and then from a 20:20:1 ratio of ethyl acetate, hexane and methanol, or, alternatively, flash chromatography on silica gel is used, employing a 100:2 mixture of methylene chloride:methanol as the mobile phase. The preferred monomers are desaminotyrosyl-tyrosine esters, including the ethyl, butyl, hexyl, octyl and benzyl esters.

In certain embodiments, some of the endcapped macromers, such as hydroxy endcapped polycaprolactone and poly(ethylene glycol), are commercially available. In some cases when endcapped macromers such as hydroxy endcapped poly(lactic acid) are not available, they may be prepared using an alkane diol as the initiator.

Monomers of the formula

may be prepared by reacting approximately two moles of one or more compounds of the formula

with approximately one mole of a compound of the formula HX³-D¹-X⁴H, wherein the variables are defined in the same manner as described elsewhere herein.

Those skilled in the art will recognize that by appropriate selection of variable groups, the compound described above is a hydroxyphenyl-alkanoic acid, such as desaminotyrosyl tyrosine (DAT), or a hydroxyphenylalkenoic acid. When the compound of the formula HX³-D¹-X⁴H is a diol, the two compounds may be reacted in an acid catalyzed Fischer esterification reaction, illustrated generally as follows:

Because this reaction is reversible, removing water from the reaction mixture shifts the equilibrium to the right. Water removal is usually accomplished by way of azeotropic distillation, however other techniques known in the art may be employed as well. In instances where azeotropic distillation is desired, the solvent used for the reaction is preferably carefully chosen so that it forms an azeotropic mixture with water. Generally, solvents such as toluene, heptane, chloroform, tetrachloethylene are preferred.

The main advantage of this reaction is that primary and secondary alcohols form esters with carboxylic acids under acid catalysis, whereas the phenolic hydroxy groups are unreactive under these conditions. Thus the carboxylic acid groups of certain compounds, such as the 3-(4-hydroxyphenyl)propionic acid (DAT) and of 3-(3,5-diiodo-4-hydroxy-phenyl)propionic acid (I₂DAT), can be reacted with primary or secondary alcohols while the phenolic groups remain intact. An example of the foregoing is as follows:

The X group in the foregoing is representative of the D and D¹groups described above. HO—X—OH can be a alkane diol such as 1,3-propane-diol or a hydroxy endcapped macromer as described above.

Polymers with a sufficient number of aromatic rings that are sufficiently substituted with bromine or iodine are inherently radiopaque. Various aromatic rings in both the first polymer phase and the second polymer phase can be iodine or bromine substituted. For example, independent of any particular polymer embodiment, the aromatic rings of the recurring units of the Formula (I-2) may be substituted with at least one iodine or bromine atom, on at least one and preferably on both ring positions. In an embodiment, at least 50% of the aromatic rings of recurring units of the Formula (I-2) in a polymer composition are substituted with from two to four iodine or bromine atoms.

The radiopaque monomers may be prepared according to the disclosure of U.S. Pat. No. 6,475,477, as well as the disclosure of U.S. Patent Publication No. 20060034769, the disclosures of both of which are incorporated herein by reference, and particularly for the purpose of describing such monomers and methods of making them. Iodinated and bromi-nated phenolic monomers described herein can also be employed as radiopacifying, bio-compatible non-toxic additives for biocompatible polymer compositions, as provided herein.

When the first monomer is a tyrosine and/or analog derived diphenol compound and the second monomer is a dihydroxy monomer (such as a diol or hydroxyl-encapped macromer) the monomers can be polymerized to form polycarbonates. Suitable processes, associated catalysts and solvents are known in the art and are taught in Schnell, Chemistry and Physics of Polycarbonates, (Interscience, New York 1964), the disclosures of which are also incorporated herein by reference. Because X³, X⁴, X⁵, X⁷, X⁸, X⁹, X¹⁰, X¹¹, X¹²and X¹³are each independently selected from the group consisting of O, S and NR¹⁰the reaction of the corresponding monomers with phosgene may also produce urethane linkages (—NR¹⁰—(C═O)—NR¹⁰—), car-bonodithioate linkages (—S—(C═O)—S—), carbamate linkages (—O—(C═O)—NR¹⁰—), thiocarbonate linkages (—S—(C═O)—O—) and thiocarbamate linkages (—S—(C═O)—NR¹⁰—). Other methods ad-aptable for use to prepare the polycarbonate and other phosgene-derived polymers disclosed herein are disclosed in U.S. Pat. Nos. 6,120,491, and 6,475,477, the disclosures of which are incorporated by reference, and particularly for the purpose of describing such methods.

The polycarbonates and other phosgene-derived polymers may also be prepared by dissolving the monomers in methylene chloride containing 0.1M pyridine or triethylamine. A solution of phosgene in toluene at a concentration between about 10 and about 25 wt %, and preferably about 20 wt %, is added at a constant rate, typically over about two hours, using a syringe pump or other means. The reaction mixture is quenched by stirring with tetrahydrofuran (THF) and water, after which the polymer is isolated by precipitation with isopropanol. Residual pyridine (if used) is then removed by agitation of a THF polymer solution with a strongly acidic resin, such as AMBERLYST 15.

Polymer compositions as described herein also include polyethers, polyesters, poly-iminocarbonates, polyphosphoesters and polyphosphazines. Those skilled in the art can prepare these polymers using routine experimentation informed by the guidance provided herein. Polyesters, specifically poly(ester amides), may be prepared by the process disclosed by U.S. Pat. No. 5,216,115, the disclosure of which is incorporated by reference, and particular-ly for the purpose of describing such processes. Polyiminocarbonates may be prepared by the process disclosed by U.S. Pat. No. 4,980,449, the disclosure of which is incorporated by reference, and particularly for the purpose of describing such processes. Polyethers may be prepared by the process disclosed by U.S. Pat. No. 6,602,497, the disclosure of which is incorporated by reference, and particularly for the purpose of describing such processes.

Preferred polymers include those having pendent free carboxylic acid groups (R₄═H). However, it is difficult to prepare polymers having pendent free carboxylic acid groups by polymerization of corresponding monomers with pendent free carboxylic acid groups without cross-reaction of the free carboxylic acid group with the co-monomer. Accordingly, polymers having pendent free carboxylic acid groups are preferably prepared from the corresponding benzyl and tert-butyl ester polymers (R₄is a benzyl or tert-butyl group).

The benzyl ester polymers may be converted to the corresponding free carboxylic acid polymers through the selective removal of the benzyl groups by the palladium catalyzed hydrogenolysis method disclosed in U.S. Pat. No. 6,120,491, the disclosure of which is incorporated herein by reference, and particularly for the purpose of describing such methods. The tert-butyl ester polymers may be converted to the corresponding free carboxylic acid polymers through the selective removal of the tert-butyl groups by the acidolyis method disclosed in U.S. Patent Publication No. 20060034769, also incorporated herein by reference, and particularly for the purpose of describing such methods. The catalytic hydrogenolysis or acidolysis is preferable because the lability of the polymer backbone tends to discourage the employment of harsher hydrolysis techniques.

The molar fraction of free carboxylic acid units in the polymers described herein can be adjusted to modify the degradation of devices made from such polymers. For example, polymers with lower amounts of free carboxylic acid will tend to have longer lifetimes in the body. Further, by otherwise adjusting the amount of free carboxylic acid in the polymers across the range of preferred molar fraction, the resulting polymers can be adapted for use in various applications requiring different device lifetimes. In general, the higher the molar fraction of free carboxylic acid units, the shorter the lifetime of the device in the body and more suitable such devices are for applications wherein shorter lifetimes are desirable or required.

Medical Uses

Various embodiments of the polymer compositions described herein, preferably derived from tissue compatible monomers, may be used to produce a variety of useful articles with valuable physical and chemical properties. The useful articles can be shaped by conventional polymer thermo-forming techniques such as extrusion and injection molding when the degradation temperature of the polymer is above the glass transition or crystalline melt temperature(s), or conventional non-thermal techniques can be used, such as compression molding, injection molding, solvent casting, spin casting, wet spinning Combinations of two or more methods can be used. Shaped articles prepared from the polymers are useful, inter alia, as biocompatible, biodegradable and/or bioresorbable biomaterials for medical implant applications.

In one embodiment, the medical device is a stent. It is contemplated that a stent may comprise many different types of forms. For instance, the stent may be an expandable stent. In another embodiment, the stent may be configured to have the form of a sheet stent, a braided stent, a self-expanding stent, a woven stent, a deformable stent, or a slide-and-lock stent. Stent fabrication processes may further include two-dimensional methods of fabrication such as cutting extruded sheets of polymer, via laser cutting, etching, mechanical cutting, or other methods, and assembling the resulting cut portions into stents, or similar methods of three-dimensional fabrication of devices from solid forms.

In certain other embodiments, the polymers are formed into coatings on the surface of an implantable device, particularly a stent, made either of a polymer as described herein or another material, such as metal. Such coatings may be formed on stents via techniques such as dipping, spray coating, combinations thereof, and the like. Further, stents may be comprised of at least one fiber material, curable material, laminated material and/or woven material. The medical device may also be a stent graft or a device used in embolotherapy.

Details of stent products and fabrication in which the polymers described herein may be employed are disclosed in U.S. Patent Publication No. 20060034769, the disclosure of which is incorporated by reference, and particularly for the purpose of describing such stent products and fabrication methods. Stents are preferably fabricated from the radiopaque polymers described herein, to permit fluoroscopic positioning of the device.

The highly beneficial combination of properties associated with preferred embodiments of the polymers described herein means these polymers are well-suited for use in producing a variety of resorbable medical devices besides stents, especially implantable medical devices that are preferably radiopaque, biocompatible, and have various times of bioresorption. For example the polymers are suitable for use in resorbable implantable devices with and without therapeutic agents, device components and/or coatings with and without therapeutic agents for use in other medical systems, for instance, the musculoskeletal or orthopedic system (e.g., tendons, ligaments, bone, cartilage skeletal, smooth muscles); the nervous system (e.g., spinal cord, brain, eyes, inner ear); the respiratory system (e.g., nasal cavity and sinuses, trachea, larynx, lungs); the reproductive system (e.g., male or female reproductive); the urinary system (e.g., kidneys, bladder, urethra, ureter); the digestive system (e.g., oral cavity, teeth, salivary glands, pharynx, esophagus, stomach, small intestine, colon), exocrine functions (biliary tract, gall bladder, liver, appendix, recto-anal canal); the endocrine system (e.g., pancreas/islets, pituitary, parathyroid, thyroid, adrenal and pineal body), the hematopoietic system (e.g., blood and bone marrow, lymph nodes, spleen, thymus, lymphatic vessels); and, the integumentary system (e.g., skin, hair, nails, sweat glands, sebaceous glands).

The polymers described herein can thus be used to fabricate wound closure devices, hernia repair meshes, gastric lap bands, drug delivery implants, envelopes for the implantation of cardiac devices, devices for other cardiovascular applications, non-cardiovascular stents such as biliary stents, esophageal stents, vaginal stents, lung-trachea/bronchus stents, and the like.

In addition, the resorbable polymers are suitable for use in producing implantable, radiopaque discs, plugs, and other devices used to track regions of tissue removal, for example, in the removal of cancerous tissue and organ removal, as well as, staples and clips suitable for use in wound closure, attaching tissue to bone and/or cartilage, stopping bleeding (homeostasis), tubal ligation, surgical adhesion prevention, and the like. Applicants have also recognized that preferred embodiments of the polymers described herein are well-suited for use in producing a variety of coatings for medical devices, especially implantable medical devices.

Further, in some preferred embodiments, the present polymers may be advantageously used in making various resorbable orthopedic devices including, for example, radiopaque biodegradable screws (interference screws), radiopaque biodegradable suture anchors, and the like for use in applications including the correction, prevention, reconstruction, and repair of the anterior cruciate ligament (ACL), the rotator cuff/rotator cup, and other skeletal deformities.

Other devices that can be advantageously formed from preferred embodiments of the polymers described herein, include devices for use in tissue engineering. Examples of suitable resorbable devices include tissue engineering scaffolds and grafts (such as vascular grafts, grafts or implants used in nerve regeneration). The resorbable polymers may also be used to form a variety of devices effective for use in closing internal wounds. For example biodegradable resorbable sutures, clips, staples, barbed or mesh sutures, implantable organ supports, and the like, for use in various surgery, cosmetic applications, and cardiac wound closures can be formed.

Various devices useful in dental applications may advantageously be formed according to embodiments of the described herein. For example devices for guided tissue regeneration, alveolar ridge replacement for denture wearers, and devices for the regeneration of maxilla-facial bones may benefit from being radiopaque so that the surgeon or dentist can ascertain the placement and continuous function of such implants by simple X-ray imaging.

Preferred embodiments of the polymers described herein are also useful in the production of bioresorbable, inherently radiopaque polymeric embolotherapy products for the temporary and therapeutic restriction or blocking of blood supply to treat tumors and vascular malformations, e.g., uterine fibroids, tumors (i.e., chemoembolization), hemorrhage (e.g., during trauma with bleeding) and arteriovenous malformations, fistulas and aneurysms delivered by means of catheter or syringe. Details of embolotherapy products and methods of fabrication in which the polymers described herein may be employed are disclosed in U.S. Patent Publication No. 20050106119 A1, the disclosure of which is incorporated by reference, and particularly for the purpose of describing such products and methods. Embolotherapy treatment methods are by their very nature local rather than systemic and the products are preferably fabricated from the radio-opaque polymers described herein, to permit fluoroscopic monitoring of delivery and treatment.

The polymers described herein are further useful in the production of a wide variety of therapeutic agent delivery devices. Such devices may be adapted for use with a variety of therapeutics including, for example, pharmaceuticals (i.e., drugs) and/or biological agents as previously defined and including biomolecules, genetic material, and processed biologic materials, and the like. Any number of transport systems capable of delivering therapeutics to the body can be made, including devices for therapeutics delivery in the treatment of cancer, intravascular problems, dental problems, obesity, infection, and the like.

A medical device that comprises a polymeric material may include one or more additional components, e.g., a plasticizer, a filler, a crystallization nucleating agent, a preservative, a stabilizer, a photoactivation agent, etc., depending on the intended application. For example, in an embodiment, a medical device comprises an effective amount of at least one therapeutic agent and/or a magnetic resonance enhancing agent. Non-limiting examples of preferred therapeutic agents include a chemotherapeutic agent, a non-steroidal anti-inflammatory, a steroidal anti-inflammatory, and a wound healing agent. Therapeutic agents may be co-administered with the polymeric material. In a preferred embodiment, at least a portion of the therapeutic agent is contained within the polymeric material. In another embodiment, at least a portion of the therapeutic agent is contained within a coating on the surface of the medical device.

Non-limiting examples of preferred chemotherapeutic agents include taxanes, taxinines, taxols, paclitaxel, dioxorubicin, cis-platin, adriamycin and bleomycin. Non-limiting examples of preferred non-steroidal anti-inflammatory compounds include aspirin, dexamethasone, ibuprofen, naproxen, and Cox-2 inhibitors (e.g., Rofexcoxib, Celecoxib and Valdecoxib). Non-limiting examples of preferred steroidal anti-inflammatory compounds include dexamethasone, beclomethasone, hydrocortisone, and prednisone. Mixtures compris-ing one or more therapeutic agents may be used. Non-limiting examples of preferred magnetic resonance enhancing agents include gadolinium salts such as gadolinium carbonate, gadolinium oxide, gadolinium chloride, and mixtures thereof.

The amounts of additional components present in the medical device are preferably selected to be effective for the intended application. For example, a therapeutic agent is preferably present in the medical device in an amount that is effective to achieve the desired therapeutic effect in the patient to whom the medical device is administered or implanted. Such amounts may be determined by routine experimentation. In certain embodiments, the desired therapeutic effect is a biological response. In an embodiment, the therapeutic agent in the medical device is selected to promote at least one biological response, preferably a biological response selected from the group consisting of thrombosis, cell attachment, cell proliferation, attraction of inflammatory cells, deposition of matrix proteins, inhibition of thrombosis, inhibition of cell attachment, inhibition of cell proliferation, inhibition of inflammatory cells, and inhibition of deposition of matrix proteins. The amount of magnetic resonance enhancing agent in a medical devices is preferably an amount that is effective to facilitate radiologic imaging, and may be determined by routine experimentation.

The term “pharmaceutical agent”, as used herein, encompasses a substance intended for mitigation, treatment, or prevention of disease that stimulates a specific physiologic (metabolic) response. The term “biological agent”, as used herein, encompasses any substance that possesses structural and/or functional activity in a biological system, including without limitation, organ, tissue or cell based derivatives, cells, viruses, vectors, nucleic acids (animal, plant, microbial, and viral) that are natural and recombinant and synthetic in origin and of any sequence and size, antibodies, polynucleotides, oligonucleotides, cDNA's, oncogenes, proteins, peptides, amino acids, lipoproteins, glycoproteins, lipids, carbohydrates, polysaccharides, lipids, liposomes, or other cellular components or organelles for instance receptors and ligands. Further the term “biological agent”, as used herein, includes virus, serum, toxin, antitoxin, vaccine, blood, blood component or derivative, allergenic product, or analogous product, or arsphenamine or its derivatives (or any trivalent organic arsenic compound) applicable to the prevention, treatment, or cure of diseases or injuries of man (per Section 351(a) of the Public Health Service Act (42 U.S.C. 262(a)). Further the term “biological agent” may include 1) “biomolecule”, as used herein, encompassing a biologically active peptide, protein, carbohydrate, vitamin, lipid, or nucleic acid produced by and purified from naturally occurring or recombinant organisms, antibodies, tissues or cell lines or synthetic analogs of such molecules; 2) “genetic material” as used herein, encompassing nucleic acid (either deoxyribonucleic acid (DNA) or ribonucleic acid (RNA), genetic element, gene, factor, allele, operon, structural gene, regulator gene, operator gene, gene complement, genome, genetic code, codon, anticodon, messenger RNA (mRNA), transfer RNA (tRNA), ribosomal extrachromosomal genetic element, plasmagene, plasmid, transposon, gene mutation, gene sequence, exon, intron, and, 3) “processed biologics”, as used herein, such as cells, tissues or organs that have undergone manipulation. The therapeutic agent may also include vitamin or mineral substances or other natural elements.

For devices placed in the vascular system, e.g., a stent, the amount of the therapeutic agent is preferably sufficient to inhibit restenosis or thrombosis or to affect some other state of the stented tissue, for instance, heal a vulnerable plaque, and/or prevent rupture or stimulate endothelialization. The agent(s) may be selected from the group consisting of antiproliferative agents, anti-inflammatory, anti-matrix metalloproteinase, and lipid lowering, cholesterol modifying, anti-thrombotic and antiplatelet agents, in accordance with preferred embodiments of the present invention. In some preferred embodiments of the stent, the therapeutic agent is contained within the stent as the agent is blended with the polymer or admixed by other means known to those skilled in the art. In other preferred embodiments of the stent, the therapeutic agent is delivered from a polymer coating on the stent surface. In another preferred variation the therapeutic agent is delivered by means of no polymer coating. In other preferred embodiments of the stent, the therapeutic agent is delivered from at least one region or one surface of the stent. The therapeutic may be chemically bonded to the polymer or carrier used for delivery of the therapeutic of at least one portion of the stent and/or the therapeutic may be chemically bonded to the polymer that comprises at least one portion of the stent body. In one preferred embodiment, more than one therapeutic agent may be delivered.

In certain embodiments, any of the aforementioned devices described herein can be adapted for use as a therapeutic delivery device (in addition to any other functionality thereof). Controlled therapeutic delivery systems may be prepared, in which a therapeutic agent, such as a biologically or pharmaceutically active and/or passive agent, is physically embedded or dispersed within a polymeric matrix or physically admixed with a polymer described herein. Controlled therapeutic agent delivery systems may also be prepared by direct application of the therapeutic agent to the surface of an implantable medical device such as a bioresorbable stent device (comprised of at least one of the polymers described herein) without the use of these polymers as a coating, or by use of other polymers or substances for the coating.

When R⁴is hydrogen the COOR⁴pendant groups of embodiments of the polymers described herein may also be derivatized by covalent attachment of a therapeutic agent. Depending on the moieties present on the underivatized therapeutic agent the covalent bond may be an amide or ester bond. Typically the therapeutic agent is derivatized at a primary or secondary amine, hydroxy, ketone, aldehyde or carboxylic acid group. Chemical attachment procedures are described by U.S. Pat. Nos. 5,219,564 and 5,660,822; Nathan et al., Bio. Cong. Chem., 4, 54-62 (1993) and Nathan, Macromol., 25, 4476 (1992), all of which are incorporated by reference, and particularly for the purpose of describing such procedures.

The therapeutic agent may first be covalently attached to a monomer, which is then polymerized, or the polymerization may be performed first, followed by covalent attachment of the therapeutic agent. Hydrolytically stable conjugates are utilized when the therapeutic agent is active in conjugated form. Hydrolyzable conjugates are utilized when the therapeutic agent is inactive in conjugated form.

Therapeutic agent delivery compounds may also be formed by physically blending the therapeutic agent to be delivered with the polymers described herein using conventional techniques well-known to those of ordinary skill in the art. For this therapeutic agent delivery embodiment, it is not essential that the polymer have pendent groups for covalent attachment of the therapeutic agent.

The polymer compositions described herein containing therapeutic agents, regardless of whether they are in the form of polymer conjugates or physical admixtures of polymer and therapeutic agent, are suitable for applications where localized delivery is desired, as well as in situations where a systemic delivery is desired. The polymer conjugates and physical admixtures may be implanted in the body of a patient in need thereof, by procedures that are essentially conventional and well-known to those of ordinary skill in the art.

Implantable medical devices may thus be fabricated that also serve to deliver a therapeutic agent to the site of implantation by being fabricated from or coated with the therapeutic agent delivery system described herein in which a polymer has a therapeutic agent physically admixed therein or covalently bonded thereto, such as a drug-eluting stent. Embolotherapeutic particles may be fabricated for delivery of a therapeutic agent.

Examples of biologically or pharmaceutically active therapeutic agents that may be covalently attached to the polymers described herein include acyclovir, cephradine, malphalen, procaine, ephedrine, adriamycin, daunomycin, plumbagin, atropine, quinine, digoxin, quinidine, biologically active peptides, chlorin e.sub.6, cephradine, cephalothin, proline and proline analogs such as cis-hydroxy-L-proline, malphalen, penicillin V and other antibiotics, aspirin and other non-steroidal anti-inflammatories, nicotinic acid, chemodeoxy-cholic acid, chlorambucil, anti-tumor and anti-proliferative agents, including anti-proliferative agents that prevent restenosis, hormones such as estrogen, and the like. Biologically active compounds, for the purposes of the present invention, are additionally defined as including cell attachment mediators, biologically active ligands, and the like.

The invention described herein also includes various pharmaceutical dosage forms containing the polymer-therapeutic agent combinations described herein. The combination may be a bulk matrix for implantation or fine particles for administration by traditional means, in which case the dosage forms include those recognized conventionally, e.g. tablets, capsules, oral liquids and solutions, drops, parenteral solutions and suspensions, emulsions, oral powders, inhalable solutions or powders, aerosols, topical solutions, suspensions, emulsions, creams, lotions, ointments, transdermal liquids and the like.

The dosage forms may include one or more pharmaceutically acceptable carriers. Such materials are non-toxic to recipients at the dosages and concentrations employed, and include diluents, solubilizers, lubricants, suspending agents, encapsulating materials, penetration enhancers, solvents, emollients, thickeners, dispersants, buffers such as phosphate, citrate, acetate and other organic acid salts, antioxidants such as ascorbic acid, preservatives, low molecular weight (less than about 10 residues) peptides such as poly-arginine, proteins such as serum albumin, gelatin, or immunoglobulins, other hydrophilic polymers such as poly(vinylpyrrolidinone), amino acids such as glycine, glutamic acid, aspartic acid, or arginine, monosaccharides, disaccharides, and other carbohydrates, including cellulose or its derivatives, glucose, mannose, or dextrines, chelating agents such as EDTA, sugar alcohols such as mannitol or sorbitol, counterions such as sodium and/or nonionic surfactants such as tween, pluronics or PEG.

Therapeutic agents to be incorporated in the polymer compositions and physical admixtures described herein may be provided in a physiologically acceptable carrier, excipient stabilizer, etc., and may be provided in sustained release or timed release formulations supplemental to the polymeric compositions described herein. Liquid carriers and diluents for aqueous dispersions are also suitable for use with the polymer compositions and physical admixtures.

Subjects in need of treatment, typically mammalian, using the polymer-therapeutic agent combinations described herein, can be administered dosages that will provide optimal efficacy. The dose and method of administration will vary from subject to subject and be dependent upon such factors as the type of mammal being treated, its sex, weight, diet, concurrent medication, overall clinical condition, the particular compounds employed, the specific use for which these compounds are employed, and other factors which those skilled in the medical arts will recognize. The polymer-therapeutic agent combinations described herein may be prepared for storage under conditions suitable for the preservation of therapeutic agent activity as well as maintaining the integrity of the polymers, and are typically suitable for storage at ambient or refrigerated temperatures.

Depending upon the particular compound selected transdermal delivery may be an op-tion, providing a relatively steady delivery of the drug, which is preferred in some circumstances. Transdermal delivery typically involves the use of a compound in solution, with an alcoholic vehicle, optionally a penetration enhancer, such as a surfactant, and other optional ingredients. Matrix and reservoir type transdermal delivery systems are examples of suitable transdermal systems. Transdermal delivery differs from conventional topical treatment in that the dosage form delivers a systemic dose of the therapeutic agent to the patient.

The polymer-drug formulations described herein may also be administered in the form of liposome delivery systems, such as small unilamellar vesicles, large unilamellar vesicles and multilamellar vesicles. Liposomes may be used in any of the appropriate routes of administration described herein. For example, liposomes may be formulated that can be administered orally, parenterally, transdermally or via inhalation. Therapeutic agent toxicity could thus be reduced by selective delivery to the affected site. For example if the therapeutic agent is liposome encapsulated, and is injected intravenously, the liposomes used are taken up by vascular cells and locally high concentrations of the therapeutic agent could be released over time within the blood vessel wall, resulting in improved action of the therapeutic agent. The liposome encapsulated therapeutic agents are preferably administered parenterally, and particularly, by intravenous injection.

Liposomes may be targeted to a particular site for release of the therapeutic agent. This would obviate excessive dosages that are often necessary to provide a therapeutically useful dosage of a therapeutic agent at the site of activity, and consequently, the toxicity and side effects associated with higher dosages.

Therapeutic agents incorporated into the polymers described herein may desirably further incorporate agents to facilitate their delivery systemically to the desired target, as long as the delivery agent meets the same eligibility criteria as the therapeutic agents described above. The active therapeutic agents to be delivered may in this fashion be incorporated with antibodies, antibody fragments, growth factors, hormones, or other targeting moieties, to which the therapeutic agent molecules are coupled.

The polymer-therapeutic agent combinations described herein may also be formed into shaped articles, such as valves, stents, tubing, prostheses, and the like. Cardiovascular stents may be combined with therapeutic agents that prevent restenosis. Implantable medical devices may be combined with therapeutic agents that prevent infection.

Therapeutically effective dosages may be determined by either in vitro or in vivo methods. For each particular drug, individual determinations may be made to determine the optimal dosage required. The range of therapeutically effective dosages will naturally be influenced by the route of administration, the therapeutic objectives, and the condition of the patient. For the various suitable routes of administration, the absorption efficiency must be individually determined for each drug by methods well known in pharmacology. Accordingly, it may be necessary for the therapist to titer the dosage and modify the route of administration as required to obtain the optimal therapeutic effect.

The determination of effective dosage levels, that is, the dosage levels necessary to achieve the desired result, will be within the ambit of one skilled in the art. Typically, applications of compound are commenced at lower dosage levels, with dosage levels being increased until the desired effect is achieved. The release rate from the formulations described herein are also varied within the routine skill in the art to determine an advantageous profile, depending on the therapeutic conditions to be treated.

A typical dosage might range from about 0.001 mg/k/g to about 1,000 mg/k/g, preferably from about 0.01 mg/k/g to about 100 mg/k/g, and more preferably from about 0.10 mg/k/g to about 20 mg/k/g. Advantageously, the polymer-therapeutic agent combinations described herein may be administered several times daily, and other dosage regimens may also be useful.

In practicing the methods described herein, the polymer-therapeutic agent combinations may be used alone or in combination with other therapeutic or diagnostic agents. The polymer-therapeutic agent combinations described herein can be utilized in vivo, ordinarily in mammals such as primates such as humans, sheep, horses, cattle, pigs, dogs, cats, rats and mice, or in vitro.

An advantage of using the radiopaque, bioresorbable polymers described herein in therapeutic agent delivery applications is the ease of monitoring release of a therapeutic agent and the presence of the implantable therapeutic delivery system. Because the radiopacity of the polymeric matrix is due to covalently attached halogen substituents, the level of radiopacity is directly related to the residual amount of the degrading therapeutic agent delivery matrix still present at the implant site at any given time after implantation. In preferred embodiments the rate of therapeutic release from the degrading therapeutic delivery system will be correlated with the rate of polymer resorption. In such preferred embodiments, the straight-forward, quantitative measurement of the residual degree of radio-opacity will provide the attending physician with a way to monitor the level of therapeutic release from the implanted therapeutic delivery system.

The following non-limiting examples set forth herein below illustrate certain aspects of the invention. All parts and percentages are by mole percent unless otherwise noted and all temperatures are in degrees Celsius unless otherwise indicated. All solvents were HPLC grade and all other reagents were of analytical grade and used as received, unless otherwise indicated.

EXAMPLES Example 1 L-tyrosine-N-[2-(4-hydroxyphenoxy)-1-oxoethyl]ethyl ester (PTE) Synthesis

Into a 1 L round-bottomed flask were added 16.8 g (0.100 mol) of (4-hydroxyphenoxy)acetic acid (HPA), 24.6 g (0.105 mol) of tyrosine ethyl ester hydrochloride (TE.HCl), 1.35 g (0.01 mol) of 1-hydroxybenzotriazole and 150 mL of tetrahydrofuran (THF). The contents of the flask were stirred while cooling to 5° C. using ice-water bath. To the stirred mixture was added 10.6 g (0.105 mol) of triethylamine followed by 21.1 g (0.110 mol) of N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDCI). This was stirred at 5° C. for 1 and then at room temperature for 5 h.

To the reaction mixture was then added 450 mL of 0.2 M HCl and stirred for 5 min and allowed to stratify. The aqueous layer was removed by siphoning and discarded. To the organic layer was added 150 mL of HCl, stirred for five minutes, allowed to stratify and the organic layer removed and discarded. The procedure was repeated with 150 mL of 5% sodium bicarbonate solution and then stirred with 250 mL of deionized water until the product solidified. The product was isolated by filtration and dried in vacuum oven at 40° C. for 24 h. The ¹H-NMR spectrum (300 MHz, DMSO-d6) was as follows: δ 9.23 (s, 1H, phenol), 8.98 (s, 1H, phenol), 8.19 (d, J=7.9, 1H, amide), 6.98 (d, J=8.4, 2H, aryl), 6.77-6.59 (m, 6H, aryl), 4.46 (d, J=5.8, 1H, α-proton), 4.35 (s, 2H, —O—CH2-HPA), 4.06 (q, J=7.1, 2H, —O—CH2-), 2.92 (t, J=6.7, 2H, —CH2-), 1.24-1.05 (m, 3H, —CH3). The melting point was 132-135° C.

Example 2 L-Tyrosine-N-[2-(4-hydroxyphenoxy)-1-oxoethyl]-hexyl ester, PTH

PTH was synthesized using procedures similar to that for PTE except in this case 26.5 g (0.105 mol) tyrosine hexyl ester (TH) was used instead of TE.HCl and triethylamine was omitted. Yield: 79 mol %, MP=94-97° C. ¹H-NMR (300 MHz, DMSO-d6) δ 9.23 (s, 1H, phenol), 8.97 (s, 1H, phenol), 8.19 (d, J=7.9, 1H, amide), 6.97 (d, J=8.4, 2H, aryl), 6.76-6.60 (m, 6H, aryl), 4.54-4.40 (m, 1H, α-proton), 4.34 (s, 2H, —O—CH2-HPA), 4.08-3.94 (m, 2H, —O—CH2-), 3.01-2.83 (m, 2H, —CH2-), 1.49 (dd, J=13.2, 6.5, 2H, —CH2-), 1.35-1.14 (m, 6H, —CH2-), 0.85 (t, J=6.7, 3H, —CH3).

Example 3 L-Tyrosine-N-[2-(4-hydroxyphenoxy)-1-oxoethyl]-Dodecyl Ester, PTD

PTD was synthesized using procedures similar to that for PTH except in this case 36.7 g (0.105 mol) tyrosine dodecyl ester (TD) was used instead of TH. Yield: 81 mol %, MP=77-80° C. ¹H-NMR (300 MHz, DMSO-d6) δ 9.22 (s, 1H, phenol), 8.97 (s, 1H, phenol), 8.19 (d, J=7.9, 1H, amide), 6.97 (d, J=8.4, 2H, aryl), 6.81-6.58 (m, 6H, aryl), 4.47 (d, J=6.1, 1H, α-proton), 4.34 (s, 2H, —O—CH2-HPA), 4.01 (t, J=6.4, 2H, —O—CH2-), 3.01-2.83 (m, 2H, —CH2-), 1.50 (s, 2H, —CH2-), 1.22 (d, J=10.3, 18H, —CH2-), 0.85 (t, J=6.6, 3H, —CH3).

Example 4 3,5-Diiodo-L-tyrosine-N-[2-(4-hydroxyphenoxy)-1-oxoethyl]ethyl ester (PI₂TE)

PI₂TE was synthesized using procedures similar to that for PTH except in this case 48.4 g (0.105 mol) 3,5-diiodo-tyrosine ethyl ester (I₂TE) was used instead of TH. In this case the I₂TE did not completely dissolve in the solvent and the reaction was carried out in suspension. The ¹H-NMR spectrum (400 MHz, DMSO-d6) was as follows: δ 9.34 (s, 1H, phenol), 8.96 (s, 1H, phenol), 8.32 (d, J=7.9, 1H, amide), 7.60 (s, 2H, aryl-I₂TE), 6.74-6.63 (m, 4H, aryl-HPA), 4.44 (d, J=5.8, 1H, α-proton), 4.35 (s, 2H, —O—CH2-HPA), 4.06 (q, J=7.1, 2H, —O—CH2-), 2.92 (t, J=6.7, 2H, —CH2-I₂TE), 1.24-1.05 (m, 3H, —CH3).

Example 5 2-((benzyloxycarbonyl)(4-hydroxyphenyl)amino)acetic acid, Z-G acid

N-(4-hydroxyphenyl)glycine (55.0 g, 0.330 mol) was dissolved in 275 mL of N-methylpyrrolidone in a 1 L round-bottomed flask. Benzyl chloroformate (50.1 mL, 0.352 mol) was added with stirring over a 40 min period and then stirred at ambient conditions for 18 h. Deionized water was added until slightly turbid and then stored at 4° C. for 24 h. The product was isolated and recrystallized in warm ethanol, dried in vacuum oven at 40° C. overnight (yield 75%, mp=196-198° C.). ¹H-NMR (500 MHz, DMSO-d6) δ 12.80 (s, 1H, COOH), 9.50 (s, 1H, phenol), 7.58-6.95 (m, 7H, aryl), 6.74 (s, 2H, aryl), 5.10 (d, J=21.9, 2H, —CH2-benzyl), 4.22 (d, J=21.7, 2H, N—CH2-).

Example 6 L-Tyrosine-N-[2-((benzyloxycarbonyl)(4-hydroxyphenyl)amino)-1-oxoethyl]-Ethyl Ester (Z-GTE)

Z-GTE was synthesized using procedures similar to that for PTE (Example 1) except in this case 30.1 g (0.105 mol) Z-G acid instead of (4-hydroxyphenoxy). Crystalline solid was obtained with an isolated yield of 80% with MP=50-55° C. ¹H-NMR (500 MHz, DMSO-d6) δ 9.45 (s, 1H, phenol), 9.23 (s, 1H, phenol), 8.32 (s, 1H, amide), 7.28 (d, J=48.2, 5H, aryl), 7.05 (ddd, J=8.7, 6.5, 2.3, 2H, aryl), 6.95 (s, 2H, aryl), 6.77-6.56 (m, 4H, aryl), 5.06 (d, J=5.7, 2H, Ph-CH2-), 4.43 (s, 1H, α-proton), 4.12 (d, J=23.6, 2H, —CH2-), 4.08-3.96 (m, 2H, —CH2-), 2.97-2.71 (m, 2H, —CH2-), 1.10 (d, J=4.8, 3H, —CH3).

Example 7 L-Tyrosine-N-[2-((benzyloxycarbonyl)(4-hydroxyphenyl)amino)-1-oxoethyl]-Hexyl Ester, Z-GTH

Z-GTH was synthesized using procedures similar to that for Z-GTE (example 6) except in this case 26.5 g (0.105 mol) tyrosine hexyl ester (TH) was used instead of TE.HCl and triethylamine was omitted. Yield: 85 mol %, MP=65-70° C. ¹H-NMR (500 MHz, DMSO-d6) δ 9.45 (s, 1H, phenol), 9.23 (s, 1H, phenol), 8.33 (s, 1H, amide), 7.28 (d, J=48.6, 5H, aryl), 7.11-7.01 (m, 2H, aryl), 6.95 (s, 2H, aryl), 6.67 (dd, J=13.5, 11.8, 4H, aryl), 5.05 (s, 2H, Ph-CH2-), 4.43 (s, 1H, α-proton), 4.13 (s, 2H, —CH2-), 3.97 (d, J=2.9, 2H, —CH2-), 2.99-2.70 (m, 2H, —CH2-), 1.46 (s, 2H, —CH2-), 1.21 (d, J=1.8, 6H, —CH2-), 0.95-0.78 (m, 3H, —CH3).

Example 8 L-Tyrosine-N-[2-((benzyloxycarbonyl)(4-hydroxyphenyl)amino)-1-oxoethyl]-Dodecyl Ester, Z-GTD

Z-GTD was synthesized using procedures similar to that for Z-GTH (example 7) except in this case 36.7 g (0.105 mol) tyrosine dodecyl ester (TD) was used instead of TH. Yield=76%; MP=87-95° C. ¹H-NMR (500 MHz, DMSO-d6) δ 9.46 (s, 1H, phenol), 9.23 (s, 1H, phenol), 8.33 (s, 1H, amide), 7.28 (d, J=45.9, 5H, aryl), 7.11-7.01 (m, 2H, aryl), 6.94 (d, J=5.1, 2H, aryl), 6.78-6.59 (m, 4H, aryl), 5.06 (d, J=6.9, 2H, Ph-CH2-), 4.44 (s, 1H, α-proton), 4.13 (s, 2H, —CH2-), 3.98 (d, J=5.5, 2H, —CH2-), 2.97-2.70 (m, 2H, —CH2-), 1.47 (s, 2H, —CH2-), 1.24 (s, 18H, —CH2-), 0.95-0.78 (m, 3H, —CH3).

Example 9 Polymerization of PTE Using Triphosgene to poly(PTE carbonate)

The reactions were carried out in a 4-necked 500 mL round-bottomed flask, equipped with an overhead stirrer, a thermometer, and a syringe pump. PTE (14.4 g, 0.040 mol), methylene chloride (115 mL), and pyridine (10.9 g, 0.14 mol) were added to the flask and stirred get a clear solution. In gas-tight glass syringe was placed a solution of syringe of 4.06 g (0.041 mol of phosgene) of triphosgene in 16 mL of methylene chloride and added to the reaction flask over 2 h using the syringe pump. The syringe was rinsed with 5 mL of methylene chloride and the rinsate was also added to the reaction flask. An aliquot of the reaction mixture was analyzed by GPC. After the desired molecular weight was reached the reaction mixture was washed 3 times with 100 mL aliquots of deionized water. If needed 5 mL of isopropanol was added to aid phase separation after each wash. The polymer was then isolated by precipitation with IPA. The precipitate was ground with IPA to remove impurities and get the polymer in the form fine powder. The product was dried in vacuum oven at 60° C. for 24 h. DSC of the polymer gave a glass transition temperature (Tg) of 92° C. The ¹H NMR spectrum of the polymer was in agreement with the structure.

Example 10

All the other monomers were polymerized to the corresponding polycarbonates using the procedures similar to the one described for Poly(PTE carbonate) above.

Example 11 Copolymers with Desaminotyrosyl Tyrosine and poly(ethylene glycol)

These polymers were prepared in example 9 except that in addition to the monomers PTR (or Z-GTR) appropriate amounts of desaminotyrosyl tyrosine tert-butyl ester (DTtBu) and poly(ethylene glycol) of MW 1000. After the completion of polymerization the DTtBu group was deprotected by adding ½ the volume of trifluoroacetic acid and stirring overnight followed by work up as in example 9.

Table of glass transition temperature of the copolymers with composition as shown


Monomer	DT (mole %)	PEG1000 (mole %)	Polymer Tg, ° C.

PTE	0	0	89.0
PTE	0	3	66.3
PTE	0	7	48.9
PTE	15	0	92.9
PTE	15	3	70.3
PTE	15	7	57.1
PTE	30	0	92.0
PTE	30	3	75.4
PTE	30	7	65.3
PTH	0	0	53.8
PTH	0	3	38.8
PTH	0	7	28.7
PTH	15	0	58.4
PTH	15	3	49.2
PTH	15	7	35.4
PTH	30	0	77.3
PTH	30	3	59.0
PTH	30	7	47.4
PTD	0	0	37.8
PTD	0	3	25.8
PTD	0	7	19.0
PTD	15	0	45.0
PTD	15	3	32.1
PTD	15	7	22.0
PTD	30	0	60.6
PTD	30	3	40.1
PTD	30	7	38.1
Z-GTE	0	0	92.5
Z-GTE	0	3	75.2
Z-GTE	0	7	68.1
Z-GTH	0	0	64.4
Z-GTH	0	3	50.2
Z-GTH	0	7	42.6
Z-GTD	0	0	58.7
Z-GTD	0	3	45.5
Z-GTD	0	7	42.1

Example 12 Hydroxy-Endcapped PLLA Macromer

The three reagents In a 100 mL round bottom flask were placed 1,3-propanediol (1.02 g, 13.4 mmol), L-lactide (36.3 g, 252 mmol) and Stannous Octoate (0.5 g, 1.26 mmol). The contents of the flask were stirred and dried under vacuum. The flask was then lowered into a silicon oil bath whose temperature was maintained between 130-140° C. The lactide began to melt and a clear liquid resulted. When observed after 2 h the reaction mixture was opaque (white), still liquid at about 130° C. The materials were allowed to react for 24 h. On cooling a white solid was obtained. The ¹H NMR showed the absence of unreacted 1,3-propanediol. GPC with THF as mobile phase (GPC, polystyrene standards) showed a bi-modal peak with Mn=3800 and Mw=7500.

Example 13 Polymerization of PLLA-Diol Using Triphosgene

Into a 250 mL round bottomed flask were added 7.50 g (0.005 mol) of PLLA-diol of Mn 1500. To the flask were also added 60 mL of methylene chloride and 1.53 g (0.019 mol) of pyridine and stirred with an overhead stirrer. To the resulting clear solution was slowly added over a period of 2 hours, 0.42 g (0.006 equivalent of phosgene) of triphosgene in 2 mL of methylene chloride, using a syringe pump. After stirring for 15 min, GPC showed a MW of 60,000. Reaction mixture washed twice with 0.2 M HCl and precipitated with methanol. The initially formed viscous oil solidified after stirring for 1 hour into a white crystalline solid. This was dried in vacuum oven at 40° C. for 24 hours.

Example 14 Poly(PHMC2K carbonate)diol

In a 1 L 4-necked flask with overhead stirrer were placed 53.4 g (27 mmol) of poly(hexamethylene carbonate 2000) (PHMC2K), 200 mL of methylene chloride and 8.23 g (0.104 mol) of pyridine. A clear solution formed on stirring. In a 20 mL sample bottle 2.33 g (3.2 eq of phosgene) of triphosgene was dissolved in 8 mL of methylene chloride and added to the reaction flask over 2 hours using the syringe pump. The reaction mixture was stirred for 15 minutes and then quenched with 250 mL of 9:1 mixture of THF-water. This was precipitated with 1500 mL of methanol in a beaker using overhead stirrer. Allowed to settle for 1 hour and then the supernatant was decanted off and discarded. The gluey precipitate at the bottom was washed with 200 mL of methanol with stirring. It was then washed with 200 mL of DI water. The residue was transferred to a PTFE dish and dried under vacuum for 24 hours at 50° C. The polymeric product became a gel during drying and hardened on cooling. DSC showed a melting point of 31.5° C.

Example 15 Polycaprolactone Diol (PCL-1300)

To a 250 mL round-bottomed flask were added 0.280 g (0.691 mmol) of Tin(II)-2-ethylhexoate, 3.64 g (0.0478 mol) of 1,3-propane diol and 57.1 g (0.500 mol) of epsilon-caprolactone. The flask was purged with dry nitrogen and then maintained under a positive nitrogen pressure. The flask was then heated in an oil bath maintained at 150° C. for 8 hours (h) while stirring using a magnetic stirrer. It was then cooled to about 40° C. and dissolved in 120 mL of tetrahydrofuran (THF). Precipitation of the solution with 240 mL of hexane gave an oily product. The oily precipitate was stirred with fresh hexane until a white waxy solid was obtained. The end group analysis using by ¹H NMR spectroscopy showed that the number average molecular weight (Mn) of the product was about 1300.

Using similar procedures and varying the molar ratios of 1,3-propane diol to that of epsilon-caprolactone, PCL-diols having molecular weights (Mn) of about 3000, about 5500, about 8400, about 10000, and about 20000 were prepared. PCL-diols may be referred to herein by the designation “PCL” followed by their approximate molecular weights, e.g., PCL-1250, PCL-3000, PCL-5500, PCL-8400, PCL-10000, PCL-20000, etc.

PCL diols having number average molecular weights (Mn) of about 1250 and about 10,000 were also prepared using 1,6-hexane diol as the initiator instead of 1,3-propane-diol.

Example 16 I₂PTE-PCL10k-PCL1.25 k Polymer

Into a 1 L 4-necked round-bottomed flask equipped with a mechanical stirrer, a syringe pump, and a thermometer is added 3,5-diiodo-2-(4-hydroxyphenoxy)-1-oxoethyl tyrosine ethyl ester (I₂PTE, 85 weight percent), 1.89 g (8 weight percent) of PCL-10000, and 1.65 g (7 weight percent) of PCL-1250. The PCL diols are prepared in the general manner described in Example 15. To the flask is added 133 mL of methylene chloride and 26.5 mL of pyridine. On stirring a clear solution resulted to which a solution of 3.8 g of triphosgene in 12 mL of methylene chloride is added over a period of 2-3 hours using the syringe pump. After the addition is complete the reaction mixture is stirred for 15 min. The resulting viscous solution is stirred with 200 mL of water and the layers are allowed to separate. The top layer is separated and discarded. The bottom layer is precipitated with 220 mL of 2-propanol in a 1 L laboratory blender. The resulting oily precipitate is hardened by repeated grinding with 2-propanol in the blender. The I₂PTE-PCL10k-PCL1.25 k polymer product is obtained in the form of powder and is dried in a vacuum oven at 40° C. to constant weight.

A number of other I₂PTE-PCL-PCL polymers are prepared using similar procedures by varying the molecular weight of the starting PCL diols by replacing the PCL-diol of Mn ˜10,000 with PCL-diols of Mn=˜3,000, ˜5,500, ˜8,400, ˜20,000, and ˜42,500. Such I₂PTE-PCL-PCL polymers may be referred to herein by including the approximate molecular weights of the PCL diols in the “I₂DTE-PCL-PCL” designation. For example, the I₂DTE-PCL-PCL polymer made using I₂DTE, PCL-10000 and PCL-1250 may be referred to herein as I₂DTE-PCL10k-PCL1.25 k.

Example 17 I₂DTE-PCL10k-PCL1.25 k Polymer

An I₂DTE-PCL10k-PCL1.25 k polymer is prepared as described in Example 17 using I₂DTE, PCL-10000, and PCL1250, except that the reaction mixture is filtered using a 40-60 micron fritted glass funnel. Precipitation of filtrate and further work up is carried out in a particle-controlled environment. The resulting I₂DTE-PCL10k-PCL1.25 k polymer is found to show improved mechanical properties as compared to a corresponding polymer not prepared under particle-controlled conditions.

Example 18 I₂DTE-PHMC12k

Into a 1 L 4-necked round-bottomed flask equipped with a mechanical stirrer, a syringe pump, and a thermometer is added 3,5-diiodo-2-(4-hydroxyphenoxy)-1-oxoethyl tyrosine ethyl ester (I₂PTE, 80 weight percent) and 5 g (20 weight percent) of poly(hexamethylene carbonate)-diol (Mn=12,000) (prepared in the manner similar to that described in Example 15). To the flask are then added 150 mL of methylene chloride and 10.3 mL of pyridine. On stirring a clear solution results to which a solution of 4.4 g of triphosgene in 17 mL of methylene chloride is added over a period of 2-3 hours using a syringe pump. After the addition is complete the reaction mixture was stirred for 15 min. The resulting viscous solution is quenched by adding a mixture of 15 mL of THF and 1.5 mL of water. After 15 min the quenched reaction mixture is precipitated with 250 mL of 2-propanol in a 1 L laboratory blender. The resulting oily precipitate is solidified by repeatedly grinding with 2-propanol in the blender. The product obtained in the form of powder is dried in a vacuum oven at 40° C. to constant weight. As indicated by the aforementioned designation “I₂DTE-PHMC12k”, polymers containing PHMC may be referred to in a manner similar to that described above for polymers containing PCL.

Example 19 PT Macromer (“HPT”, PT diester of 1,-6-hexane diol)

Into a 500 mL round-bottomed flask equipped with an overhead stirrer, a Dean-Stark trap and a thermometer is added 11.8 g (0.10 mol) of 1,6-hexanediol, 0.202 mol of PT, 1.90 g (0.01 mol) of p-toluenesulfonic acid, and 200 mL of heptane. The flask is heated using a heating mantle while stirring with the overhead stirrer. About 3.8 mL of water collects in the Dean-Stark trap and it does not increase further. Heating is stopped and the reaction mixture allowed to cool to room temperature. The supernatant is removed by decantation and the residue in the flask is dried in a current of nitrogen. The off-white precipitate is dissolved in 100 mL of acetone and precipitated by repeated washing with 5% NaHCO₃solution. The solid is washed with deionized water (DI), isolated by filtration and dried. The product is further purified by extraction with diethyl ether (10 mL/g of solid) to provide the HDAT macromer product.

Example 20 Poly(HPT carbonate)-diol Macromer

In a 1 L 4-necked flask with overhead stirrer is placed 22.8 mmol of the HPT prepared in the Example 20, 80 mL of methylene chloride and 6.8 g (86 mmol) of pyridine. A clear solution forms on stirring. In a 20 mL sample bottle 2.37 g of triphosgene (24.0 mmol of phosgene) is dissolved in 8 mL of methylene chloride and added to the reaction flask over 2 h using a syringe pump. The reaction mixture is stirred for 15 m and then quenched by stirring with 100 mL of water. This is precipitated with 135 mL of IPA in a beaker using overhead stirrer. The oily precipitate is triturated with several portions of IPA when the product is obtained as a solid. The residue is transferred to a PTFE dish and dried under vacuum for 24 h at 50° C.

Example 21 I₂PTE-HPT12k Polymer

Into a 1 L 4-necked round-bottomed flask equipped with a mechanical stirrer, a syringe pump, and a thermometer is added 3,5-diiodo-2-(4-hydroxyphenoxy)-1-oxoethyl tyrosine ethyl ester (I₂PTE, 90 wt. %) and 10 wt. % poly(HPT carbonate)-diol. To the flask is then added 180 mL of methylene chloride and 10.2 mL of pyridine. On stirring a clear solution results to which a solution of 3.8 g of triphosgene in 12 mL of methylene chloride is added over a period of 2-3 hours using a syringe pump. After the addition is complete the reaction mixture is stirred for 15 min. The resulting viscous solution is quenched by adding a mixture of 15 mL THF and 1.5 mL water. After 15 min the quenched reaction mixture is precipitated with 250 mL 2-propanol in a 1 L laboratory blender. The resulting oily precipitate is solidified by repeatedly grinding with 2-propanol in the blender. The product is obtained in the form of powder and is dried in a vacuum oven at 40° C. to constant weight.

Example 22 I₂PTE-PCL10K Using Propane Diol as Plasticizer/Plastifier

Into a 1 L 4-necked round-bottomed flask equipped with a mechanical stirrer, a syringe pump, and a thermometer is added 3,5-diiodo-2-(4-hydroxyphenoxy)-1-oxoethyl tyrosine ethyl ester (I₂PTE, 85 weight percent), 1.89 g (8 weight percent) PCL10K and 1.65 g propane diol (7 weight percent). To the flask is then added 133 mL methylene chloride and 16.8 mL pyridine. On stirring a clear solution results to which a solution of 5.9 g of triphosgene in 18 mL of methylene chloride is added over a period of 2-3 h using a syringe pump. After the addition is complete the reaction mixture is stirred for 15 min. The resulting viscous solution is quenched by adding a mixture of 15 mL THF and 1.5 mL water. After 15 min the quenched reaction mixture is precipitated with 250 mL 2-propanol in a 1 L laboratory blender. The resulting oily precipitate is solidified by repeated grinding with 2-propanol in the blender. The product obtained in the form of powder is dried in a vacuum oven at 40° C. to constant weight.

Example 23 Preparation of Acyl-Imine-Containing Polycarbonates By Reacting Monomer with Excess Triphosgene

In a 500 mL round bottomed flask is placed 0.042 mol 2-(4-hydroxyphenoxy)-1-oxo-ethyl tyrosine ethyl ester (PTE), 0.011 g (0.07 mmol) ethyl 4-hydroxybenzoate (end-capping agent), 12.45 g (0.16 mol) pyridine, and 150 mL methylene chloride and stirred under a nitrogen atm. Triphosgene (6.65 g, 0.067 mol phosgene) is dissolved in 37 mL methylene chloride and the solution is added to the flask using a syringe pump over a 2 h period. After the addition is complete the reaction mixture is stirred for 17 h. The reaction mixture is precipitated with 1 L 2-propanol in a 4 L blender. The resulting gel like product is ground repeatedly with 0.5 L 2-propanol. The solid product is isolated by filtration and ground with Deionized water and finally dried in a vacuum oven

Example 24 Preparation of an Acyl-Imine-Containing Polycarbonate

In a 250 mL round bottomed flask 12 placed poly(PTE carbonate) (0.026 mol repeat units), pyridine (2.57 g, 0.026 mol) and 100 mL methylene chloride. To the resulting solution is added with stirring a solution of triphosgene (1.29 g, 0.013 mol phosgene) in 5 mL of methylene chloride over a 90 min period. The reaction mixture is stirred at ambient temperature for 24 h and precipitated with 500 mL 2-propanol in a blender. The resulting yellow polymer is washed twice with 250 mL portions of 2-propanol, isolated by filtration and dried in vacuum oven at 40° C. The polymer was characterized by ¹H NMR, and gel permeation chromatography.

It will be understood by those of skill in the art that numerous and various modifications can be made without departing from the spirit of the present invention. Therefore, it should be clearly understood that the various embodiments of the present invention described herein are illustrative only and not intended to limit the scope of the present invention.

Claims

What is claimed is:

1. A compound of Formula (I):

wherein:

i is an integer selected from 1 through 4;

y¹and y²are each independently selected from 0, 1, 2, 3 and 4;

X¹and X², at each occurrence, are independently bromine (Br) or iodine (I);

X is oxygen (O), sulfur (S), or NR⁴; where R⁴is selected from the group consisting of hydrogen and alkyl containing from 1 to 6 carbon atoms;

R³is an optionally substituted C_1-30alkyl; and

R⁶is selected from the group consisting of hydrogen, alkyl, aryl, alkyaryl, heteroalkyl and heteroalkylaryl, wherein the non-hydrogen groups contain up to 30 carbon atoms, wherein the heteroalkyl group contains from 1 to 10 heteroatoms independently selected from O, NR³and S, and wherein the heteroalkylaryl group contains from 1 to 3 heteroatoms independently selected from O, N³and S;

wherein the and —X¹and —OH groups on the left phenyl ring, at each occurrence, are independently at o-, m-, or p-positions.

2. A biocompatible polymer, comprising a repeating unit of Formula (I-1):

wherein;

i is an integer selected from 1 through 4;

y¹and y²are each independently selected from 0, 1, 2, 3 and 4;

X¹and X², at each occurrence, are independently bromine (Br) or iodine (I);

R³is selected from the group consisting of hydrogen and optionally substituted C_1-30alkyl; and

R⁶is selected from the group consisting of hydrogen, alkyl, aryl, alkylaryl, heteroalkyl and heteroalkylaryl, wherein the non-hydrogen groups contain up to 30 carbon atoms, wherein the heteroalkyl group contains from 1 to 10 heteroatoms independently selected from O, NR³and S, and wherein the heteroalkylaryl group contains from 1 to 3 heteroatoms independently selected from O, NR ³and S;

wherein the —X¹and groups on the left phenyl ring, at each occurrence, are independently at o-, m-, or p-positions.

3. A biocompatible polymer, comprising a repeating unit of Formula (I-2):

wherein:

i is an integer selected from 1 through 4;

y¹and y²are each independently selected from 0, 1, 2, 3 and 4;

X¹and X², at each occurrence, are independently bromine (Br) or iodine (I);

R³is selected from the group consisting of hydrogen and optionally substituted C_1-30alkyl;

R⁶is selected from the group consisting of hydrogen, alkyl, aryl, alkylaryl, heteroalkyl and heteroalkylaryl, wherein the non-hydrogen groups contain up to 30 carbon atoms, wherein the heteroalkyl group contains from 1 to 10 heteroatoms independently selected from O, NR³and S, and wherein the heteroalkylaryl group contains from 1 to 3 heteroatoms independently selected from O, NR³and S; and

A¹is selected from:

R¹⁰is selected from the group consisting of H, C₁-C₃₀alkyl, alkenyl, alkynyl, C₂-C₃₀heteroalkyl, heteroalkenyl and heteroalkynyl; and

R¹²is selected from the group consisting of a bond, C₁-C₃₀alkylene, alkenylene, alkynylene, C₁-C₃₀heteroalkylene, heteroalkenylene, heteroalkynylene, C₅-C₃₀heteroalkylarylene, heteroalkenylarylene, heteroalkynylarylene, C₆-C₃₀alkylarylene, alkenylarylene, alkynylarylene, and C₅-C₃₀heteroarylene;

wherein the —X¹and —O—groups on the left phenyl ring, at each occurrence, are independently at o-, m-, or p-positions.

4. The biocompatible polymer according to claim 3, wherein A¹is

5. The biocompatible polymer of claim 3, further comprising:

a second polymer recurring unit of Formula (III):

and

a third polymer recurring unit of Formula (IV):

wherein:

j is an integer in the range from 1 to about 500;

y¹and y²are each independently selected from 0, 1, 2, 3 and 4;

X¹and X², at each occurrence, are independently bromine (Br) or iodine (I);

A^lis selected from:

wherein the amounts of unit (I-2), unit (III), and unit (IV) are in a;b;c ratio, wherein each of a, b, and c is a number between 0 to 1, inclusive, provided that a+b+c=1, and a is not equal to 0; and

6. The biocompatible polymer according to claim 5, wherein A¹is

7. A biocompatible polymer composition, comprising at least a first polymer phase and a second polymer phase;

the first polymer phase comprising a polymer according to claim 3, said polymer having at least one first wet thermal transition temperature selected from a first wet glass transition temperature and a first wet melting point, the first wet thermal transition temperature being at least 38° C.;

the first polymer phase comprising a number (n) of first recurring units of Formula (I-2); and

wherein the number (n) and the number (m) are selected to control the relative amounts of the first polymer phase and the second polymer phase so that (a) the polymer composition is phase-separated over at least the temperature range of about 25° C. to about 50° C., (b) the polymer composition has a water content of 4.5% w/w or less as measured after soaking for 24 hours at 37° C. in 0.1 M phosphate buffered saline (PBS) at pH 7.4; and (c) the volume fraction of the second polymer phase in the polymer composition is in the range of about 6% to about 40%, based on total volume.

8. The polymer composition of claim 7, wherein A¹is

9. The polymer composition of claim 7, wherein the number (n) and the number (m) are further selected to control the ductility properties of the polymer composition, such that the polymer composition has an elongation at break of greater than 30%, a Young's modulus of greater than 130 ksi and a strength at yield of greater than 4.0 ksi, when tested under tensile test conditions that comprise (i) providing a tensile test strip having measurements of 0.2 inches wide, a gauge length of 1.0 inches and a thickness of 0.004 inches, (ii) aging the tensile test strip for 7 days at 37° C. in 0.1 M phosphate buffered saline (PBS) at pH 7.4, and (iii) pulling the aged tensile test strip at a rate of 10 inches per minute while submerged in water at 37° C.

10. The polymer composition of claim 7, wherein the polymer composition comprises a block copolymer, the block copolymer comprising at least a first block and a second block, wherein the block copolymer is phase-separated so that more than about half of the first block is in the first polymer phase and more than about half of the second block is in the second polymer phase.

11. The polymer composition of claim 7, wherein the second recurring units have a formula selected from the group consisting of Formula (IIa), Formula (IIb), Formula (IIc), and Formula (IId):

wherein X³, X⁴, X⁵, X⁷, X⁸, X⁹, X¹⁰, X¹¹, X¹²and X¹³are each independently selected from the group consisting of O, S and NR¹⁰, where R¹⁰is selected from hydrogen and an alkyl group containing from one to 30 carbon atoms;

Ar¹and Ar²are phenylene rings optionally substituted with from one to four substituents independently selected from the group consisting of halogen, halomethyl, halomethoxy, methyl, methoxy, thiomethyl, nitro, sulfoxide, and sulfonyl;

R⁸and R⁹contain from one to ten carbon atoms each and are independently selected from the group consisting of an optionally substituted alkylene, an optionally substituted heteroalkylene, an optionally substituted alkenylene, and an optionally substituted heteroalkenylene;

g and h in Formula (IId) are each independently integers in the range of about 1 to about 500; and

D and D¹contain up to 24 carbon atoms and are independently selected from the group consisting of an optionally substituted alkylene, an optionally substituted heteroalkylene, an optionally substituted alkenylene and an optionally substituted heteroalkenylene;

wherein the recurring unit of formula (IIa) is obtained from a compound of formula HX⁸-D-X⁹H, which is a hydroxyl and endcapped macromere, a mercapto endcapped macromer, or an amino endcapped macromer, and wherein the recurring unit of formula (IIc) is obtained from a compound HX³-D¹-X⁴H defines a hydroxyl endcapped macromer, a mercapto endcapped macromer or an amino endcapped macromer.

12. The polymer composition of claim 11, wherein HX³-D¹-X⁴H and HX⁸-D-X⁹H are each independently a macromer selected from a hydroxy endcapped polylactic acid macromer, a hydroxy endcapped polyglycolic acid macromer, a hydroxy endcapped poly(lactic acid-co-glycolic acid) macromer, a hydroxy endcapped polycaprolactone macromer, a poly(alkylene diol) macromer, a hydroxy end-capped poly(alkylene oxide) macromer and a hydroxy endcapped polydioxanone macromer.

13. The polymer composition of claim 11, further comprising third recurring units having a Formula selected from the group consisting of Formula (IIa), Formula (IIb), Formula (IIc), and Formula (IId), wherein said third recurring units differ from said second recurring units.

14. The polymer composition of claim 13, wherein said second recurring units and said third recurring units both have a structure Formula (IId), wherein the variables g and h for said second recurring units differ from the variables g and h for said third recurring units.

15. The polymer composition of claim 14, wherein for Formula (IId), X³, X⁴, X⁵, X⁷, X¹¹, X¹², and X¹³are all O; R⁸and R⁹are both —(CH₂)₅—, and D¹is C₁-C₂₄alkylene.

16. The polymer composition of claim 15, wherein X¹is I; y¹=2; y²=0; A¹is

and g and h for the second recurring units of Formula (IId) are each independently integers such that the sum of g+h is in the range of about 10 to about 15.

17. The polymer composition of claim 7, wherein the first recurring units of Formula (I-2) of the first polymer phase is selected to contain sufficient halogen atoms to render the polymer composition inherently radiopaque.

18. A medical device comprising the biocompatible polymer or polymer composition according to claim 9.

19. The medical device of claim 18, wherein the polymer composition further comprises a biologically active compound.

20. The medical device of claim 19, wherein the bioactive agent is selected from the group consisting of a chemotherapeutic agent, a non-steroidal anti-inflammatory, a steroidal anti-inflammatory, and a wound healing agent.

21. The medical device of claim 18, wherein the medical device is a stent.

22. The medical device of claim 21, wherein the number (n) and the number (m) are further selected to control the fatigue properties of the polymer composition, such that the polymer composition remains intact for at least 60 minutes when tested under fatigue test conditions that comprise (i) providing a fatigue test strip having measurements of 5.0 mm wide, a gauge length of 15 mm and a thickness of 0.1 mm, (ii) aging the fatigue test strip for 7 days at 37° C. in 0.1 M phosphate buffered saline (PBS) at pH 7.4, and (iii) subjecting the aged fatigue test strip to oscillating deformation at a frequency of 1.2 Hz under a stress of 10 MPa in single frequency stress mode while submerged in water at 37° C.

23. A medical device comprising the polymer composition of claim 16.

24. The medical device of claim 23, wherein the medical device is a stent.

25. The medical device of claim 24, wherein the number (n) and the number (m) are further selected to control the fatigue properties of the polymer composition, such that the polymer composition remains intact for at least 60 minutes when tested under fatigue test conditions that comprise (i) providing a fatigue test strip having measurements of 5.0 mm wide, a gauge length of 15 mm and a thickness of 0.1 mm, (ii) aging the fatigue test strip for 7 days at 37° C. in 0.1 M phosphate buffered saline (PBS) at pH 7.4 and (iii) subjecting the aged fatigue test strip to oscillating deformation at a frequency of 1.2 Hz under a stress of 10 MPa in single frequency stress mode while submerged in water at 37° C.

26. The biocompatible polymer of claim 3, comprising:

a number (n) of first polymer recurring units of Formula (I-2); and

a number (m) of second polymer recurring units, effective to result in phase separation of said polymer into first and second polymer phases, wherein said second phase comprises said second polymer recurring units;

wherein the polymer has a water content of 4.5% w/w or less as measured after soaking for 24 hours at 37° C. in 0.1 M phosphate buffered saline (PBS) at pH 7.4; and the second polymer recurring units and the number values for (n) and (m) are selected so that said polymer remains intact for at least about 15 minutes when tested under fatigue test conditions that comprise (i) providing a fatigue test strip having measurements of 5.0 mm wide, a gauge length of 15 mm and a thickness of 0.1 mm, (ii) aging the fatigue test strip for 7 days at 37° C. in 0.1 M phosphate buffered saline (PBS) at pH 7.4, and (iii) subjecting the aged fatigue test strip to oscillating deformation at a frequency of 1.2 Hz under a stress of 10 MPa in single frequency stress mode while submerged in water at 37° C.

27. The polymer of claim 26, further comprising third polymer recurring units selected from the group consisting of linear C₁-C₃₀alkyl, branched C₁-C₃₀alkyl, linear C₁-C₃₀poly(alkylene diol), and branched C₁-C₃₀poly(alkylene oxide).

28. The polymer of claim 26, wherein the second polymer recurring unit has a formula selected from the group consisting of Formula (IIa), Formula (IIb) and Formula (IIc):

wherein X³, X⁴, X⁵, X⁸, X⁹, X¹⁰, X¹¹, X¹²and X¹³are independently selected from the group consisting of O, S and NR¹⁰, where R¹⁰is selected from hydrogen and an alkyl group containing from one to 30 carbon atoms;

R⁸and R⁹contain from one to ten carbon atoms each and are independently selected from the group consisting of an optionally substituted alkylene, an optionally substituted heteroalkylene, an optionally substituted alkenylene, and an optionally substituted heteroalkenylene; and

D and D¹contain up to 24 carbon atoms and are independently selected from the group consisting of an optionally substituted alkylene, an optionally substituted heteroalkylene, an optionally substituted alkenylene and an optionally substituted heteroalkenylene,

wherein the recurring unit of formula (IIa) is obtained from a compound of formula HX⁸-D-X⁹H, which is a hydroxyl endcapped macromer, a mercapto endcapped macromer, or an amino endcapped macromer, and wherein the recurring unit of formula (IIc) is obtained from a compound of formula HX³-D¹-X⁴H, which is a hydroxyl endcapped macromer, a mercapto endcapped macromer, or an amino endcapped macromer.