US3632754A - Use of chitin for promoting wound healing - Google Patents
Use of chitin for promoting wound healing Download PDFInfo
- Publication number
- US3632754A US3632754A US704538A US3632754DA US3632754A US 3632754 A US3632754 A US 3632754A US 704538 A US704538 A US 704538A US 3632754D A US3632754D A US 3632754DA US 3632754 A US3632754 A US 3632754A
- Authority
- US
- United States
- Prior art keywords
- chitin
- wound healing
- healing
- wound
- rats
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0024—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid beta-D-Glucans; (beta-1,3)-D-Glucans, e.g. paramylon, coriolan, sclerotan, pachyman, callose, scleroglucan, schizophyllan, laminaran, lentinan or curdlan; (beta-1,6)-D-Glucans, e.g. pustulan; (beta-1,4)-D-Glucans; (beta-1,3)(beta-1,4)-D-Glucans, e.g. lichenan; Derivatives thereof
- C08B37/0027—2-Acetamido-2-deoxy-beta-glucans; Derivatives thereof
- C08B37/003—Chitin, i.e. 2-acetamido-2-deoxy-(beta-1,4)-D-glucan or N-acetyl-beta-1,4-D-glucosamine; Chitosan, i.e. deacetylated product of chitin or (beta-1,4)-D-glucosamine; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/28—Polysaccharides or their derivatives
Definitions
- This invention relates to methods of promoting the healing of wounds and compositions therefor comprising chitin, and/ or chitin derivatives and/or partially depolymerized chitin.
- one aspect of the present invention relates to novel methods of promoting and assisting the healing of wounds as, for example, damaged mammalian tissue, open ulcers, etc., and to compositions therefor.
- Another aspect of the invention relates to significant improvements in wound healing strength achieved by the administration of finely divided chitin, partially depolymerized chitin or chitin derivatives to a patient.
- An additional aspect of the present invention is concerned with articles of manufacture such as surgical bandages, surgical sutures, etc., containing the wound healing materials of the present invention.
- Chitin is a polysaccharide, believed to be poly (N-acetylglucosamine) which forms the cell walls of fungi and the hard shell of insects and crustaceans.
- the term chitin embraces naturally occurring chitin, synthetic chitin, as well as poly (N-acetylglucosamine) and its epimer poly (N-acetylgalactosamine).
- the partially depolymerized chitin e.g. chitotriose, chitobiose, is a substance which retains its polymeric nature but has undergone a reduction in molecular weight (i.e. chain length) as a result of (1) enzymatic action such as by a chitinase enzyme, (2) chemical treatment such as acid hydrolysis or alkaline treatment, and (3) physical treatment.
- the chitin derivatives contemplated are materials such as ethers formed with pharmaceutically-acceptable radi cals and esters or salts with pharmaceutically-acceptable acids.
- suitable derivatives include hydroxy lower alkyl chitin such as hydroxyethyl chitin, carboxy alkyl chitin such as carboxymethyl chitin, salts of carboxy lower alkyl chitin such as the zinc salt, lower alkyl chitin such as methyl chitin and ethyl chitin, chitin acetate, chitin nitrate, chitin citrate, chitin phosphate, N-acyl derivatives derived from monocarboxylic aliphatic acids such as N-formyl, N-acetyl, N-propionyl, N-caproyl, etc.
- the naturally occurring chitin is preferably chitin of fungal origin, both by reason of its ready availability and its high degree of effectiveness.
- the degree of improvement in wound healing obtained with the chitin materials is at least equal to and in many instances greater than that derived from the cartilage materials of the prior art.
- the substantial improvement in rate of healing which is obtained from the use of poly (l I- acetylglucosamine), i.e., chitin, as compared to monomeric N-acetylglucosamine is particularly surprising.
- chitin, particularly chitin of fungal origin is a relatively uniform and easily obtained material.
- compositions of the present invention are applied using the same techniques and processes developed for cartilage, and N-acetylglucosamine.
- tablets, capsules or pellets of chitin may be prepared from mixtures of chitin, partially depolymerized chitin or chitin derivatives with wellknown pharmaceutical excipients such as starch, sugar, certain forms of clay, etc. Such tablets, capsules or pellets may be taken orally or implanted near the situs of the wound.
- a colloidal solution may be prepared from chitin, preferably in isotonic saline, or a watersoluble derivative of chitin may be dissolved preferably in isotonic saline solution, and the solution administered intramuscularly, parenterally or intravenously.
- a powder or solution of chitin or of a chitin derivative may also be used to impregnate a surgical gauze or pad which is applied to the wound.
- Chitin may also be dissolved as the alkali chitin xanthate, spun into fibers and regenerated as the virtually undegraded polymer in accordance with the procedures described the prior art by Thor et a1.
- Partially deacetylated chitin filaments and fibers may be prepared in accordance with the procedure described in US. Pat. No. 2,040,880. These chitin fibers may then be used as surgical sutures or included in bandages or other support base for surgical dressings either in a woven or nonwoven fabric structure in the manner described in US. Pat.
- Chitin or chitin derivative may also be made up into an ointment or salve.
- the use of nonactive carriers for the chitin is not preferred as the presence of extraneous matter in a wound frequently tends to interfere with the healing process due to the interposition effect.
- chitin is to be applied by injection, i.e., either intramuscularly, parenterally or intravenously, it is first necessary to prepare a dispersion or a solution of the material in a pharmaceutically acceptable liquid.
- Colloidal solutions of chitin may be prepared using the method described by Lingappa and Lockwood in Nature, 189, page 158 (1961).
- isotonic solution such as isotonic saline.
- the chitin or chitin derivatives may be used alone, in admixture with each other, with cartilage, or may be coadministered with other therapeutically eifective agents such as ascorbic acid, ascorbyl palmitate, pharmaceutically acceptable zinc salts such as zinc oxide, zinc ascorbate, zinc sulfate and zinc stearate; antiseptics such as thimerosol and benzalkonium chloride; local anesthetics such as lidocaine and procaine; antibiotics such as chloramphenicol, sulfanilamide and ampicilline. Combinations of the therapeutically effective agents described above with chitin and/ or chitin derivatives may be used.
- therapeutically eifective agents such as ascorbic acid, ascorbyl palmitate, pharmaceutically acceptable zinc salts such as zinc oxide, zinc ascorbate, zinc sulfate and zinc stearate
- antiseptics such as thimerosol and benzalkonium chloride
- Suitable sources of chitin are from lobsters, shrimp and other crustacea. To utilize chitin from such sources, it is necessary to reduce the chitin in particle size to less than about 150 microns and preferably less than about 50 microns. Due to the tough and rather fibrous nature of chitin from such sources, this grinding is difficult and expensive. Accordingly, it is preferred to use chitin of fungal origin. The cell walls of fungi are made of chitin. It has been found that it is not necessary to extract the chitin from the remaining cell material.
- the entire fungal mat produced by fermentation of a fungus in a suitable nutrient medium may be ground and used to promote healing of wounds.
- the fungal mat is treated to remove the extraneous materials leaving only the chitin skeletons. Purifying the material in this manner eliminates the nonchitinous materials, thus substantially reducing the possibility of an allergic reaction and eliminating any interference with the healing process which might be cause by such materials.
- Finely divided chitin or chitin derivatives may be ap plied topically by blowing a metered amount of the material onto the wound using a hand atomizer. Alternatively, it may be applied by dusting as from a hand shaker or may be placed together with an inert gas under increased pressure (i.e., above atmospheric pressure) in a pressure vessel.
- the finely divided chitin or chitin derivative may be packaged as a dry aerosol powder as de scribed in Dutch patent application 6,415,252, published July 5, 1965 (this patent application is directed to a medicament for bovine mastitis but the method of aerosol packaging described is applicable to powdered medicament having the described particle size) or as an aerosol foam.
- the wound healing efliciency of the various chitinous materials is determined by using the method of Prudden et a1. as described above.
- EXAMPLE 1 Commercial lobster shell chitin is ground to a fine powder in a laboratory four-quart size porcelain jar mill loaded with one-inch size (average) flint pebbles in a weight ratio of 1 chitin to 2 pebbles. Dry Ice is then put on top of the mill charge and the mill is kept open for 5 minutes to allow the CO to displace the air in the mill. The lid of the mill is then clamped on tight and the grinding carried out for 96 hours. Approximately 50% of the powdered chitin passed through a 40 micron screen.
- the whole powdered chitin so produced is then applied to the 45 test rats of 45 pairs of rats used in the Prudden et al. assay method described above.
- the percent of wound healing for the treated rats, stating the control rats as 100%, is 122%, i.e., the use of chitin results in an average 22% increase in wound healing activity.
- EXAMPLES 2-5 Various fungi are grown on either brain-heart infusion (200 gm. calf brain, 250 gm. beef heart, 10 gm. proteose peptone, 2 gm. dextrose, 5 gm. sodium chloride and 2.5 gm. disodium phosphate) called BHI or on Sabourauds broth (40 gm. dextrose and 10 gm. bacto-peptone) called SAB.
- the cultures are grown in shallow layers of media contained in flasks and held stationary until good growth and extensive sporulation occurs. Prior to collection of the growth mats, the cultures are killed by placing the flasks into a closed oven under CO at 127 C. for three hours.
- the flasks are then cooled in the oven for an additional one hour and fifteen minutes.
- Culture broths are removed by filtration through Buchner funnels and the growth mats washed with distilled water.
- the mats are then frozen and lyophilized and the dry products ground in a mortar with a pestle under CO No attempt is made to purify the chiten. Twelve pairs of rats are used for each test. Some inflammation is observed on all treated wounds and infection on several. The increases in wound healing obtained may be all the more significant in view of those adverse factors.
- 100 grams of dried fungus material (obtained from Penicillium fungus of Example 4, cultured on a BHI medium, sterilized by boiling the fungus with the medium and then filtering, washing with distilled water and drying the fungus material) is defatted by extracting the solventsoluble fatty materials with 1000 ml. chloroform at room temperature. The chloroform is removed by filtering and then drying at reduced pressure in a vacuum desiccator.
- the defatted fungus material is treated with 2000 ml. 1.0 N-NaOH solution for 18 hours at room temperature. The material is then acidified with HCl. Thereafter the material is dialyzed in distilled water unitl the wash water is free from chlorine ions. This procedure is repeated until a substantially purified material is obtained. The material is dried in a vacuum below 50 C. and is a gray, friable mass.
- the dried material is ground in a laboratory mortar and screened through a 400 mesh standard screen.
- the screened material is applied to 20 test rats of 20 pairs of rats there is obtained an average of about 25% increase in the wound healing of the treated rats over the untreated control rats.
- Lobster shell chitin is purified by first sl-urrying it in 10% aqueous NaOH for minutes at 80 C., then it is washed, drained and slurried in HCl for 5 minutes at 80 C., drained, slurried in water, the pH of the water adjusted to 6 with dilute aqueous NaOH, and finally drained and dried.
- the dried chitin material is pulverized to a fineness of about 40 microns.
- the material shows an average 25% increase in the wound healing over the untreated control rats.
- a process for facilitating healing of a wound in a mammal which comprises administering to said mammal a therapeutically effective amount of chitin.
- said material of fungal origin is a sterilized, defatted material selected 6 from the class consisting of Mucor spinosus, Aspergillus niger, Penicillium and Cryptococcus.
- a process for facilitating healing of a wound in a mammal which comprises topically applying to said Wound in said mammal a therapeutically effective amount of chitin.
Abstract
WOUND HEALING COMPOSITION AND THE PROCESS OF HEALING WOUNDS WITH SUCH COMPOSITIONS ARE DESCRIBED, THE COMPOSITIONS CONTAINING CHITIN, PARTIALLY DEPOLYMERIZED CHITIN OR A CHITIN DERIVATIVE.
Description
UnitedStates Patent 3,632,754 USE OF CHITIN FOR PROMOTING WOUND HEALING Leslie L. Balassa, Blooming Grove, N.Y., assignor t0 Lescarden Ltd., Goshen, N.Y.
N0 Drawing. Continuation-impart of application Ser. No. 619,007, Feb. 27, 1967. This application Feb. 12, 1968, Ser. No. 704,538
Int. Cl. A61k 17/00 US. Cl. 424-180 6 Claims ABSTRACT OF THE DISCLOSURE Wound healing compositions and the process of healing wounds with such compositions are described, the compositions containing chitin, partially depolymerized chitin or a chitin derivative.
This application is a continuation-in-part of my copending application Ser. No. 619,007 filed Feb. 27, 1967.
This invention relates to methods of promoting the healing of wounds and compositions therefor comprising chitin, and/ or chitin derivatives and/or partially depolymerized chitin.
Medicine has long been interested in improving the healing of wounds. Patients suffering from diabetes or undergoing extensive cortisone treatment show extremely slow rates of healing of any wounds which they receive. Thus, surgery on such patients involves additional risks not present with other patients. Moreover, rapid healing of wounds is particularly desired for patients in tropical countries where the risk of infection is high. Rapid healing is also desired in the case of soldiers who have been wounded in a battle zone and cannot easily and quickly be removed therefrom. Acceleration of wound healing is highly desirable in the case of patients who cannot readily be immobilized, such as farm animals.
In evaluating the utility of a material to promote wound healing, a reproducible test is necessary to give comparative data. Such a test method has been described by Prudden et al. in: The Acceleration of Wound Healing With Cartilage, Surgery, Gynecology and Obstetrics, 105 2283 (1957). In this method, rats are tested in pairs, each pair receiving an identical surgical incision, only the one rat of the pair receiving a measured dose of the material whose wound healing properties is to be determined. The pair is then kept in the same cage and the tensile strength of the wounds in the two rats is determined in millimeters of mercury. The difference in the tensile strengths between the treated rat and the control rat is expressed as the percentage improvement obtained. Considering biological variance it is believed that only differences of about 10% or more are significant.
There have been several recent developments reported concerning materials which promote wound healing. In this connection US. Pat. No. 3,232,836 describes the parenteral administration of N-acetylglucosamine as a wound healing material. Utilizing the test method of Prudden et al. referred to in the preceding paragraph, N-acetylglucosamine showed improvement in tensile strength of only about 10% whereas Prudden and his co-workers have reported significantly larger increases in wound healing by the use of cartilage preparations from various animals. Depending on the age and species of animal and the fineness of the cartilage powder, improvements ranging from to 40% in wound healing tensile strength have been reported by Prudden.
Now it has been discovered that finely divided chitin, partially depolymerized chitin, and chitin derivatives possess the ability to promote the healing of wounds.
3,632,754 Patented Jan. 4, 1972 ice Accordingly, one aspect of the present invention relates to novel methods of promoting and assisting the healing of wounds as, for example, damaged mammalian tissue, open ulcers, etc., and to compositions therefor.
Another aspect of the invention relates to significant improvements in wound healing strength achieved by the administration of finely divided chitin, partially depolymerized chitin or chitin derivatives to a patient.
An additional aspect of the present invention is concerned with articles of manufacture such as surgical bandages, surgical sutures, etc., containing the wound healing materials of the present invention.
These and other aspects of the present invention will be apparent from the following description.
Chitin is a polysaccharide, believed to be poly (N-acetylglucosamine) which forms the cell walls of fungi and the hard shell of insects and crustaceans. As used herein, the term chitin embraces naturally occurring chitin, synthetic chitin, as well as poly (N-acetylglucosamine) and its epimer poly (N-acetylgalactosamine). The partially depolymerized chitin, e.g. chitotriose, chitobiose, is a substance which retains its polymeric nature but has undergone a reduction in molecular weight (i.e. chain length) as a result of (1) enzymatic action such as by a chitinase enzyme, (2) chemical treatment such as acid hydrolysis or alkaline treatment, and (3) physical treatment.
The chitin derivatives contemplated are materials such as ethers formed with pharmaceutically-acceptable radi cals and esters or salts with pharmaceutically-acceptable acids. Examples of suitable derivatives include hydroxy lower alkyl chitin such as hydroxyethyl chitin, carboxy alkyl chitin such as carboxymethyl chitin, salts of carboxy lower alkyl chitin such as the zinc salt, lower alkyl chitin such as methyl chitin and ethyl chitin, chitin acetate, chitin nitrate, chitin citrate, chitin phosphate, N-acyl derivatives derived from monocarboxylic aliphatic acids such as N-formyl, N-acetyl, N-propionyl, N-caproyl, etc.
It is preferred to use natural chitin as the wound healing accelerator. The naturally occurring chitin is preferably chitin of fungal origin, both by reason of its ready availability and its high degree of effectiveness.
The degree of improvement in wound healing obtained with the chitin materials is at least equal to and in many instances greater than that derived from the cartilage materials of the prior art. The substantial improvement in rate of healing which is obtained from the use of poly (l I- acetylglucosamine), i.e., chitin, as compared to monomeric N-acetylglucosamine is particularly surprising. As compared to the great variability in cartilage depending on the animal, its age and the method of collecting the cartilage, chitin, particularly chitin of fungal origin, is a relatively uniform and easily obtained material.
The compositions of the present invention are applied using the same techniques and processes developed for cartilage, and N-acetylglucosamine. Thus, it is preferred to topically apply finely divided chitin directly to the wound surface. However, tablets, capsules or pellets of chitin may be prepared from mixtures of chitin, partially depolymerized chitin or chitin derivatives with wellknown pharmaceutical excipients such as starch, sugar, certain forms of clay, etc. Such tablets, capsules or pellets may be taken orally or implanted near the situs of the wound. Alternatively, a colloidal solution may be prepared from chitin, preferably in isotonic saline, or a watersoluble derivative of chitin may be dissolved preferably in isotonic saline solution, and the solution administered intramuscularly, parenterally or intravenously.
A powder or solution of chitin or of a chitin derivative may also be used to impregnate a surgical gauze or pad which is applied to the wound. Chitin may also be dissolved as the alkali chitin xanthate, spun into fibers and regenerated as the virtually undegraded polymer in accordance with the procedures described the prior art by Thor et a1. Partially deacetylated chitin filaments and fibers may be prepared in accordance with the procedure described in US. Pat. No. 2,040,880. These chitin fibers may then be used as surgical sutures or included in bandages or other support base for surgical dressings either in a woven or nonwoven fabric structure in the manner described in US. Pat. 3,196,075. Chitin or chitin derivative may also be made up into an ointment or salve. The use of nonactive carriers for the chitin is not preferred as the presence of extraneous matter in a wound frequently tends to interfere with the healing process due to the interposition effect.
As previously stated, where the chitin is to be applied by injection, i.e., either intramuscularly, parenterally or intravenously, it is first necessary to prepare a dispersion or a solution of the material in a pharmaceutically acceptable liquid. Colloidal solutions of chitin may be prepared using the method described by Lingappa and Lockwood in Nature, 189, page 158 (1961). When administered intravenously it is preferred to administer the compound in isotonic solution such as isotonic saline.
The chitin or chitin derivatives may be used alone, in admixture with each other, with cartilage, or may be coadministered with other therapeutically eifective agents such as ascorbic acid, ascorbyl palmitate, pharmaceutically acceptable zinc salts such as zinc oxide, zinc ascorbate, zinc sulfate and zinc stearate; antiseptics such as thimerosol and benzalkonium chloride; local anesthetics such as lidocaine and procaine; antibiotics such as chloramphenicol, sulfanilamide and ampicilline. Combinations of the therapeutically effective agents described above with chitin and/ or chitin derivatives may be used.
Suitable sources of chitin are from lobsters, shrimp and other crustacea. To utilize chitin from such sources, it is necessary to reduce the chitin in particle size to less than about 150 microns and preferably less than about 50 microns. Due to the tough and rather fibrous nature of chitin from such sources, this grinding is difficult and expensive. Accordingly, it is preferred to use chitin of fungal origin. The cell walls of fungi are made of chitin. It has been found that it is not necessary to extract the chitin from the remaining cell material. Thus, if desired, after suitable sterilization as by heat or gas (i.e., ethylene oxide), the entire fungal mat produced by fermentation of a fungus in a suitable nutrient medium may be ground and used to promote healing of wounds. Preferably, however, the fungal mat is treated to remove the extraneous materials leaving only the chitin skeletons. Purifying the material in this manner eliminates the nonchitinous materials, thus substantially reducing the possibility of an allergic reaction and eliminating any interference with the healing process which might be cause by such materials.
Finely divided chitin or chitin derivatives may be ap plied topically by blowing a metered amount of the material onto the wound using a hand atomizer. Alternatively, it may be applied by dusting as from a hand shaker or may be placed together with an inert gas under increased pressure (i.e., above atmospheric pressure) in a pressure vessel. In this latter means of application, termed aerosol application, the finely divided chitin or chitin derivative, optionally with other medicaments as indicated, may be packaged as a dry aerosol powder as de scribed in Dutch patent application 6,415,252, published July 5, 1965 (this patent application is directed to a medicament for bovine mastitis but the method of aerosol packaging described is applicable to powdered medicament having the described particle size) or as an aerosol foam.
In the following examples, the wound healing efliciency of the various chitinous materials is determined by using the method of Prudden et a1. as described above.
4 In general, at least 10 pairs of rats are used to obtain a meaningful average for each material tested. In each of these examples a powder insufliator is used to apply 2 to 10 mg./cm. of wound surface of the material tested.
EXAMPLE 1 Commercial lobster shell chitin is ground to a fine powder in a laboratory four-quart size porcelain jar mill loaded with one-inch size (average) flint pebbles in a weight ratio of 1 chitin to 2 pebbles. Dry Ice is then put on top of the mill charge and the mill is kept open for 5 minutes to allow the CO to displace the air in the mill. The lid of the mill is then clamped on tight and the grinding carried out for 96 hours. Approximately 50% of the powdered chitin passed through a 40 micron screen.
The whole powdered chitin so produced is then applied to the 45 test rats of 45 pairs of rats used in the Prudden et al. assay method described above. The percent of wound healing for the treated rats, stating the control rats as 100%, is 122%, i.e., the use of chitin results in an average 22% increase in wound healing activity.
EXAMPLES 2-5 Various fungi are grown on either brain-heart infusion (200 gm. calf brain, 250 gm. beef heart, 10 gm. proteose peptone, 2 gm. dextrose, 5 gm. sodium chloride and 2.5 gm. disodium phosphate) called BHI or on Sabourauds broth (40 gm. dextrose and 10 gm. bacto-peptone) called SAB. The cultures are grown in shallow layers of media contained in flasks and held stationary until good growth and extensive sporulation occurs. Prior to collection of the growth mats, the cultures are killed by placing the flasks into a closed oven under CO at 127 C. for three hours. The flasks are then cooled in the oven for an additional one hour and fifteen minutes. Culture broths are removed by filtration through Buchner funnels and the growth mats washed with distilled water. The mats are then frozen and lyophilized and the dry products ground in a mortar with a pestle under CO No attempt is made to purify the chiten. Twelve pairs of rats are used for each test. Some inflammation is observed on all treated wounds and infection on several. The increases in wound healing obtained may be all the more significant in view of those adverse factors.
100 grams of dried fungus material (obtained from Penicillium fungus of Example 4, cultured on a BHI medium, sterilized by boiling the fungus with the medium and then filtering, washing with distilled water and drying the fungus material) is defatted by extracting the solventsoluble fatty materials with 1000 ml. chloroform at room temperature. The chloroform is removed by filtering and then drying at reduced pressure in a vacuum desiccator.
The defatted fungus material is treated with 2000 ml. 1.0 N-NaOH solution for 18 hours at room temperature. The material is then acidified with HCl. Thereafter the material is dialyzed in distilled water unitl the wash water is free from chlorine ions. This procedure is repeated until a substantially purified material is obtained. The material is dried in a vacuum below 50 C. and is a gray, friable mass.
The dried material is ground in a laboratory mortar and screened through a 400 mesh standard screen. When the screened material is applied to 20 test rats of 20 pairs of rats there is obtained an average of about 25% increase in the wound healing of the treated rats over the untreated control rats.
EXAMPLE 7 Lobster shell chitin is purified by first sl-urrying it in 10% aqueous NaOH for minutes at 80 C., then it is washed, drained and slurried in HCl for 5 minutes at 80 C., drained, slurried in water, the pH of the water adjusted to 6 with dilute aqueous NaOH, and finally drained and dried.
The dried chitin material is pulverized to a fineness of about 40 microns. The material shows an average 25% increase in the wound healing over the untreated control rats.
Although the present invention has been described in conjunction with preferred embodiments, it is to be understood that modifications and variations may be resorted to without departing from the spirit and scope thereof, as those skilled in the art will readily understand.
What is claimed is:
1. A process for facilitating healing of a wound in a mammal which comprises administering to said mammal a therapeutically effective amount of chitin.
2. A process according to claim 1, wherein said chitin is of fungal origin.
3. A process according to claim 1 wherein said chitin is of crustacea origin.
4. A process according to claim 2 wherein said material of fungal origin is a sterilized, defatted material selected 6 from the class consisting of Mucor spinosus, Aspergillus niger, Penicillium and Cryptococcus.
5. A process according to claim 2, wherein the chitin consists of the purified cell walls of fungi.
6. A process for facilitating healing of a wound in a mammal which comprises topically applying to said Wound in said mammal a therapeutically effective amount of chitin.
References Cited UNITED STATES PATENTS ALBERT T. MEYERS, Primary Examiner D. M. STEPHENS, Assistant Examiner US. Cl. X.R. 424
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US70453868A | 1968-02-12 | 1968-02-12 |
Publications (1)
Publication Number | Publication Date |
---|---|
US3632754A true US3632754A (en) | 1972-01-04 |
Family
ID=24829928
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US704538A Expired - Lifetime US3632754A (en) | 1968-02-12 | 1968-02-12 | Use of chitin for promoting wound healing |
Country Status (1)
Country | Link |
---|---|
US (1) | US3632754A (en) |
Cited By (60)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3911098A (en) * | 1974-02-11 | 1975-10-07 | American Cyanamid Co | Medicament carrier |
US3982537A (en) * | 1974-12-30 | 1976-09-28 | Louis Bucalo | Dynamic implants and method for implanting the same |
US3988411A (en) * | 1974-02-11 | 1976-10-26 | American Cyanamid Company | Spinning and shaping poly-(N-acetyl-D-glucosamine) |
US3989535A (en) * | 1974-02-11 | 1976-11-02 | American Cyanamid Company | Solution of poly(N-acetyl-D-glucosamine) |
US4074713A (en) * | 1975-03-14 | 1978-02-21 | American Cyanamid Company | Poly(N-acetyl-D-glucosamine) products |
US4086335A (en) * | 1975-10-29 | 1978-04-25 | Bruscato Frank N | Pharmaceutical tablets containing chitin as a disintegrant |
US4120527A (en) * | 1977-04-15 | 1978-10-17 | Caterpillar Tractor Co. | Cab design |
EP0002506A1 (en) * | 1977-12-09 | 1979-06-27 | Wella Aktiengesellschaft | Use of compositions for conditioning as well as washing hair |
US4486416A (en) * | 1981-03-02 | 1984-12-04 | Soll David B | Protection of human and animal cells subject to exposure to trauma |
DE3342886A1 (en) * | 1983-11-26 | 1985-06-05 | The University of Delaware, Newark, Del. | Wound healing agent, its composition and use |
US4532267A (en) * | 1984-02-15 | 1985-07-30 | Board Of Regents, University Of Washington | Vision correction lens made from an aminopolysaccharide compound or an ether or ester thereof |
US4532134A (en) * | 1981-04-06 | 1985-07-30 | Malette William Graham | Method of achieving hemostasis, inhibiting fibroplasia, and promoting tissue regeneration in a tissue wound |
US4572906A (en) * | 1983-11-21 | 1986-02-25 | Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of National Defence Of Her Majesty's Canadian Government | Chitosan based wound dressing materials |
DE3437184A1 (en) * | 1984-10-10 | 1986-04-17 | Eduard Iwan | Composition for the treatment of skin burns and a process for the preparation of this composition |
WO1986005789A1 (en) * | 1985-04-01 | 1986-10-09 | Biocarb Ab | Carbohydrate derivatives and compositions thereof for therapeutic or diagnostic use, and methods for their use |
US4623539A (en) * | 1983-02-04 | 1986-11-18 | Tunc Deger C | Nutrient barrier polysaccharide compositions and method of use |
US4920158A (en) * | 1989-10-11 | 1990-04-24 | Medipro Sciences Limited | Hydrogel-forming wound dressing or skin coating material |
US4938950A (en) * | 1986-12-23 | 1990-07-03 | Wella Aktiengesellschaft | Cosmetic composition based upon chitosan and ampholytic copolymerizates, as well as new chitosan/polyampholyte salts |
EP0412744A1 (en) * | 1989-08-08 | 1991-02-13 | Unilever Plc | Hair treatment composition |
EP0412745A1 (en) * | 1989-08-08 | 1991-02-13 | Unilever Plc | Hair setting composition |
US4994277A (en) * | 1989-10-31 | 1991-02-19 | Pfizer Hospital Products Group, Inc. | Use of xanthan gum for preventing adhesions |
US5013769A (en) * | 1988-08-22 | 1991-05-07 | Medipro Sciences Limited | Method of making a hydrogel-forming wound dressing or skin coating material |
US5093319A (en) * | 1989-10-31 | 1992-03-03 | Pfizer Hospital Products Group, Inc. | Use of derivatives of chitin soluble in aqueous solutions for preventing adhesions |
US5141925A (en) * | 1990-04-23 | 1992-08-25 | Trustees Of Tufts College | Vivo methods for treating coccidiosis |
US5300494A (en) * | 1986-06-06 | 1994-04-05 | Union Carbide Chemicals & Plastics Technology Corporation | Delivery systems for quaternary and related compounds |
US5605938A (en) * | 1991-05-31 | 1997-02-25 | Gliatech, Inc. | Methods and compositions for inhibition of cell invasion and fibrosis using dextran sulfate |
US5705178A (en) * | 1991-05-31 | 1998-01-06 | Gliatech, Inc. | Methods and compositions based on inhibition of cell invasion and fibrosis by anionic polymers |
US5733884A (en) * | 1995-11-07 | 1998-03-31 | Nestec Ltd. | Enteral formulation designed for optimized wound healing |
EP0957925A1 (en) * | 1996-12-13 | 1999-11-24 | Lescarden, Inc. | Treatment of osteoarthritis by administering poly-n-acetyl-d-glucosamine |
US6150581A (en) * | 1995-06-07 | 2000-11-21 | United States Surgical Corporation | Chitosan/alginate anti-adhesion barrier |
US20030093114A1 (en) * | 2001-11-13 | 2003-05-15 | Melvin Levinson | Method for effecting hemostasis |
US20030148998A1 (en) * | 2001-02-16 | 2003-08-07 | Cargill, Incorporated | Glucosamine and method of making glucosamine from microbial biomass |
US6693188B2 (en) | 2001-08-08 | 2004-02-17 | Cargill Incorporated | N-acetyl-D-glucosamine and process for producing N-acetyl-D-glucosamine |
US20040077055A1 (en) * | 2001-02-16 | 2004-04-22 | Cargill, Incorporated | Glucosamine and method of making glucosamine from microbial biomass |
US20040243043A1 (en) * | 2002-06-14 | 2004-12-02 | Mccarthy Simon J, | Wound dressing and method for controlling severe, life-threatening bleeding |
US20050038369A1 (en) * | 2001-06-14 | 2005-02-17 | Gregory Kenton W. | Wound dressing and method for controlling severe, life-threatening bleeding |
US20050215774A1 (en) * | 2002-04-02 | 2005-09-29 | Trinkle Jamea R | Chitosan production |
US20050245482A1 (en) * | 2000-03-15 | 2005-11-03 | Weiyu Fan | Chitosan and method of preparing chitosan |
US20060003965A1 (en) * | 2002-11-01 | 2006-01-05 | Fosdick Lawrence D | N-acetyl-d-glucosamine (nag) supplemented food products and beverages |
US20060004314A1 (en) * | 2001-06-14 | 2006-01-05 | Hemcon, Inc. | Antimicrobial barriers, systems, and methods formed from hydrophilic polymer structures such as chistosan |
US20060058263A1 (en) * | 2002-11-01 | 2006-03-16 | Rogers Brent D | Heat pasturized liquids containing glucosamine |
US20060134220A1 (en) * | 2002-06-20 | 2006-06-22 | Bioalliance Pharma | Vectorization system comprising nanoparticles of homogenous size of at least one polymer and at least one positively charged polysaccharide and method for the preparation thereof |
US20060172392A1 (en) * | 2001-02-16 | 2006-08-03 | Cargill, Incorporated | Water soluble beta-glucan, glucosamine, and N-acetylglucosamine compositions and methods for making the same |
US20060178344A1 (en) * | 2001-02-16 | 2006-08-10 | Cargill, Incorporated | Glucosamine and N-acetylglucosamine and methods of making the same fungal biomass |
US20060211973A1 (en) * | 2005-03-17 | 2006-09-21 | Gregory Kenton W | Systems and methods for hemorrhage control and or tissue repair |
US20060246114A1 (en) * | 2002-11-01 | 2006-11-02 | Rogers Brent D | Multiple component food product useful for delivering glucosamine and/or nacetyl-d-glucosamine |
US20080146984A1 (en) * | 2002-06-14 | 2008-06-19 | Hemcon Medical Technologies, Inc. | Method for preparing a compressed wound dressing |
US20080287907A1 (en) * | 2006-05-23 | 2008-11-20 | Providence Health System-Oregan D/B/A Providence St. Vincent Medical Center | Systems and methods for introducing and applying a bandage structure within a body lumen or hollow body organ |
US20090018479A1 (en) * | 2002-06-14 | 2009-01-15 | Hemcon Medical Technologies, Inc. | Absorbable tissue dressing assemblies, systems, and methods formed from hydrophilic polymer sponge structures such as chistosan |
US20090048210A1 (en) * | 2004-05-21 | 2009-02-19 | Saburo Minami | Agent for therapy or treatment of wound |
US20090104284A1 (en) * | 2007-10-19 | 2009-04-23 | Gunn Jr Herbert Harrell | Topically Applied Chitosan Composition for Pain Relief and Boosting Immune System |
US20100172958A1 (en) * | 2008-05-02 | 2010-07-08 | Hemcon Medical Technologies, Inc. | Wound dressing devices and methods |
US20100228185A1 (en) * | 2008-12-22 | 2010-09-09 | Abbott Laboratories | Carriers for hemostatic tract treatment |
US20100323986A1 (en) * | 2001-02-12 | 2010-12-23 | Marine Polymer Technologies, Inc., | Compositions and methods for modulation of vascular structure and/or function |
WO2011026869A2 (en) | 2009-09-01 | 2011-03-10 | Medovent Gmbh | Tissue dressing kit |
WO2013109004A1 (en) | 2012-01-19 | 2013-07-25 | 주식회사 시지바이오 | Antimicrobial wound-covering material and method for manufacturing same |
US8715719B2 (en) | 2010-06-16 | 2014-05-06 | Abbott Vascular, Inc. | Stable chitosan hemostatic implant and methods of manufacture |
US8741335B2 (en) | 2002-06-14 | 2014-06-03 | Hemcon Medical Technologies, Inc. | Hemostatic compositions, assemblies, systems, and methods employing particulate hemostatic agents formed from hydrophilic polymer foam such as Chitosan |
US8992453B2 (en) | 2003-09-12 | 2015-03-31 | Marine Polymer Technologies, Inc. | Vascular access preservation in hemodialysis patients |
US10086105B2 (en) | 2008-10-06 | 2018-10-02 | Providence Health System—Oregon | Chitosan foam medical devices and methods |
-
1968
- 1968-02-12 US US704538A patent/US3632754A/en not_active Expired - Lifetime
Cited By (100)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3911098A (en) * | 1974-02-11 | 1975-10-07 | American Cyanamid Co | Medicament carrier |
US3988411A (en) * | 1974-02-11 | 1976-10-26 | American Cyanamid Company | Spinning and shaping poly-(N-acetyl-D-glucosamine) |
US3989535A (en) * | 1974-02-11 | 1976-11-02 | American Cyanamid Company | Solution of poly(N-acetyl-D-glucosamine) |
US3982537A (en) * | 1974-12-30 | 1976-09-28 | Louis Bucalo | Dynamic implants and method for implanting the same |
US4074713A (en) * | 1975-03-14 | 1978-02-21 | American Cyanamid Company | Poly(N-acetyl-D-glucosamine) products |
US4074366A (en) * | 1975-03-14 | 1978-02-21 | American Cyanamid Company | Poly(N-acetyl-D-glucosamine) products |
US4086335A (en) * | 1975-10-29 | 1978-04-25 | Bruscato Frank N | Pharmaceutical tablets containing chitin as a disintegrant |
US4120527A (en) * | 1977-04-15 | 1978-10-17 | Caterpillar Tractor Co. | Cab design |
EP0002506A1 (en) * | 1977-12-09 | 1979-06-27 | Wella Aktiengesellschaft | Use of compositions for conditioning as well as washing hair |
US4486416A (en) * | 1981-03-02 | 1984-12-04 | Soll David B | Protection of human and animal cells subject to exposure to trauma |
US4532134A (en) * | 1981-04-06 | 1985-07-30 | Malette William Graham | Method of achieving hemostasis, inhibiting fibroplasia, and promoting tissue regeneration in a tissue wound |
US4623539A (en) * | 1983-02-04 | 1986-11-18 | Tunc Deger C | Nutrient barrier polysaccharide compositions and method of use |
US4572906A (en) * | 1983-11-21 | 1986-02-25 | Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of National Defence Of Her Majesty's Canadian Government | Chitosan based wound dressing materials |
DE3342886A1 (en) * | 1983-11-26 | 1985-06-05 | The University of Delaware, Newark, Del. | Wound healing agent, its composition and use |
WO1985003716A1 (en) * | 1984-02-15 | 1985-08-29 | Washington Research Foundation | Vision correction lens made from an aminopolysaccharide compound or an ether or ester thereof |
US4532267A (en) * | 1984-02-15 | 1985-07-30 | Board Of Regents, University Of Washington | Vision correction lens made from an aminopolysaccharide compound or an ether or ester thereof |
DE3437184A1 (en) * | 1984-10-10 | 1986-04-17 | Eduard Iwan | Composition for the treatment of skin burns and a process for the preparation of this composition |
WO1986005789A1 (en) * | 1985-04-01 | 1986-10-09 | Biocarb Ab | Carbohydrate derivatives and compositions thereof for therapeutic or diagnostic use, and methods for their use |
US5300494A (en) * | 1986-06-06 | 1994-04-05 | Union Carbide Chemicals & Plastics Technology Corporation | Delivery systems for quaternary and related compounds |
US4938950A (en) * | 1986-12-23 | 1990-07-03 | Wella Aktiengesellschaft | Cosmetic composition based upon chitosan and ampholytic copolymerizates, as well as new chitosan/polyampholyte salts |
US5013769A (en) * | 1988-08-22 | 1991-05-07 | Medipro Sciences Limited | Method of making a hydrogel-forming wound dressing or skin coating material |
JPH0460964B2 (en) * | 1989-08-08 | 1992-09-29 | Unilever Nv | |
JPH0377812A (en) * | 1989-08-08 | 1991-04-03 | Unilever Nv | Hair tonic compound |
JPH0377811A (en) * | 1989-08-08 | 1991-04-03 | Unilever Nv | Hair treatment compound |
EP0412745A1 (en) * | 1989-08-08 | 1991-02-13 | Unilever Plc | Hair setting composition |
JPH0460578B2 (en) * | 1989-08-08 | 1992-09-28 | Unilever Nv | |
EP0412744A1 (en) * | 1989-08-08 | 1991-02-13 | Unilever Plc | Hair treatment composition |
US4920158A (en) * | 1989-10-11 | 1990-04-24 | Medipro Sciences Limited | Hydrogel-forming wound dressing or skin coating material |
US4994277A (en) * | 1989-10-31 | 1991-02-19 | Pfizer Hospital Products Group, Inc. | Use of xanthan gum for preventing adhesions |
US5093319A (en) * | 1989-10-31 | 1992-03-03 | Pfizer Hospital Products Group, Inc. | Use of derivatives of chitin soluble in aqueous solutions for preventing adhesions |
US5141925A (en) * | 1990-04-23 | 1992-08-25 | Trustees Of Tufts College | Vivo methods for treating coccidiosis |
US6083930A (en) * | 1991-05-31 | 2000-07-04 | Gliatech Inc. | Methods and compositions based on inhibition of cell invasion and fibrosis by anionic polymers |
US5705178A (en) * | 1991-05-31 | 1998-01-06 | Gliatech, Inc. | Methods and compositions based on inhibition of cell invasion and fibrosis by anionic polymers |
US5705177A (en) * | 1991-05-31 | 1998-01-06 | Gliatech Inc. | Methods and compositions based on inhibition of cell invasion and fibrosis by anionic polymers |
US5994325A (en) * | 1991-05-31 | 1999-11-30 | Gliatech Inc. | Methods and compositions based on inhibition of cell invasion and fibrosis by anionic polymers |
US6020326A (en) * | 1991-05-31 | 2000-02-01 | Gliatech Inc. | Method for inhibition of bone growth by anionic polymers |
US5605938A (en) * | 1991-05-31 | 1997-02-25 | Gliatech, Inc. | Methods and compositions for inhibition of cell invasion and fibrosis using dextran sulfate |
US6127348A (en) * | 1991-05-31 | 2000-10-03 | Gliatech, Inc. | Methods and compositions based on inhibition of cell invasion and fibrosis by anionic polymers |
US6150581A (en) * | 1995-06-07 | 2000-11-21 | United States Surgical Corporation | Chitosan/alginate anti-adhesion barrier |
US5733884A (en) * | 1995-11-07 | 1998-03-31 | Nestec Ltd. | Enteral formulation designed for optimized wound healing |
EP0957925A1 (en) * | 1996-12-13 | 1999-11-24 | Lescarden, Inc. | Treatment of osteoarthritis by administering poly-n-acetyl-d-glucosamine |
US6117851A (en) * | 1996-12-13 | 2000-09-12 | Lescarden Inc. | Treatment of osteoarthritis by administering poly-N-acetyl-D-glucosamine |
EP0957925A4 (en) * | 1996-12-13 | 2002-07-24 | Lescarden Inc | Treatment of osteoarthritis by administering poly-n-acetyl-d-glucosamine |
US20050245482A1 (en) * | 2000-03-15 | 2005-11-03 | Weiyu Fan | Chitosan and method of preparing chitosan |
US7413881B2 (en) | 2000-03-15 | 2008-08-19 | Cargill, Incorporated | Chitosan and method of preparing chitosan |
US8481512B2 (en) | 2001-02-12 | 2013-07-09 | Marine Polymer Technologies, Inc. | Compositions and methods for modulation of vascular structure and/or function |
US8859528B2 (en) | 2001-02-12 | 2014-10-14 | Marine Polymer Technologies, Inc. | Compositions and methods for modulation of vascular structure and/or function |
US20100323986A1 (en) * | 2001-02-12 | 2010-12-23 | Marine Polymer Technologies, Inc., | Compositions and methods for modulation of vascular structure and/or function |
US20030148998A1 (en) * | 2001-02-16 | 2003-08-07 | Cargill, Incorporated | Glucosamine and method of making glucosamine from microbial biomass |
US20040077055A1 (en) * | 2001-02-16 | 2004-04-22 | Cargill, Incorporated | Glucosamine and method of making glucosamine from microbial biomass |
US8222232B2 (en) | 2001-02-16 | 2012-07-17 | Cargill, Incorporated | Glucosamine and N-acetylglucosamine compositions and methods of making the same fungal biomass |
US8034925B2 (en) | 2001-02-16 | 2011-10-11 | Cargill, Incorporated | Glucosamine and method of making glucosamine from microbial biomass |
US20060178344A1 (en) * | 2001-02-16 | 2006-08-10 | Cargill, Incorporated | Glucosamine and N-acetylglucosamine and methods of making the same fungal biomass |
US7923437B2 (en) | 2001-02-16 | 2011-04-12 | Cargill, Incorporated | Water soluble β-glucan, glucosamine, and N-acetylglucosamine compositions and methods for making the same |
US7816514B2 (en) | 2001-02-16 | 2010-10-19 | Cargill, Incorporated | Glucosamine and method of making glucosamine from microbial biomass |
US20060172392A1 (en) * | 2001-02-16 | 2006-08-03 | Cargill, Incorporated | Water soluble beta-glucan, glucosamine, and N-acetylglucosamine compositions and methods for making the same |
US20110143312A1 (en) * | 2001-06-14 | 2011-06-16 | Hemcon Medical Technologies, Inc. | Compositions, assemblies, and methods applied during or after a dental procedure to ameliorate fluid loss and/or promote healing, using a hydrophilic polymer sponge structure such as chitosan |
US9132206B2 (en) | 2001-06-14 | 2015-09-15 | Hemcon Medical Technologies, Inc. | Hemostatic compositions, assemblies, systems, and methods employing particulate hemostatic agents formed from hydrophilic polymer foam such as chitosan |
US9004918B2 (en) | 2001-06-14 | 2015-04-14 | Hemcon Medical Technologies, Inc. | Compositions, assemblies, and methods applied during or after a dental procedure to ameliorate fluid loss and/or promote healing, using a hydrophilic polymer sponge structure such as chitosan |
US20050038369A1 (en) * | 2001-06-14 | 2005-02-17 | Gregory Kenton W. | Wound dressing and method for controlling severe, life-threatening bleeding |
US20060004314A1 (en) * | 2001-06-14 | 2006-01-05 | Hemcon, Inc. | Antimicrobial barriers, systems, and methods formed from hydrophilic polymer structures such as chistosan |
US7482503B2 (en) | 2001-06-14 | 2009-01-27 | Providence Health System-Oregon | Wound dressing and method for controlling severe, life-threatening bleeding |
US6693188B2 (en) | 2001-08-08 | 2004-02-17 | Cargill Incorporated | N-acetyl-D-glucosamine and process for producing N-acetyl-D-glucosamine |
US20030093114A1 (en) * | 2001-11-13 | 2003-05-15 | Melvin Levinson | Method for effecting hemostasis |
US20090099347A1 (en) * | 2002-04-02 | 2009-04-16 | Cargill, Incorporated | Chitosan production |
US20050215774A1 (en) * | 2002-04-02 | 2005-09-29 | Trinkle Jamea R | Chitosan production |
US7488812B2 (en) | 2002-04-02 | 2009-02-10 | Cargill, Incorporated | Chitosan production |
US8741335B2 (en) | 2002-06-14 | 2014-06-03 | Hemcon Medical Technologies, Inc. | Hemostatic compositions, assemblies, systems, and methods employing particulate hemostatic agents formed from hydrophilic polymer foam such as Chitosan |
US8668924B2 (en) | 2002-06-14 | 2014-03-11 | Providence Health System—Oregon | Wound dressing and method for controlling severe, life-threatening bleeding |
US20080146984A1 (en) * | 2002-06-14 | 2008-06-19 | Hemcon Medical Technologies, Inc. | Method for preparing a compressed wound dressing |
US7371403B2 (en) * | 2002-06-14 | 2008-05-13 | Providence Health System-Oregon | Wound dressing and method for controlling severe, life-threatening bleeding |
US20090018479A1 (en) * | 2002-06-14 | 2009-01-15 | Hemcon Medical Technologies, Inc. | Absorbable tissue dressing assemblies, systems, and methods formed from hydrophilic polymer sponge structures such as chistosan |
US8313474B2 (en) | 2002-06-14 | 2012-11-20 | Hemcon Medical Technologies, Inc. | Method for preparing a compressed wound dressing |
US8269058B2 (en) | 2002-06-14 | 2012-09-18 | Hemcon Medical Technologies, Inc. | Absorbable tissue dressing assemblies, systems, and methods formed from hydrophilic polymer sponge structures such as chitosan |
US20040243043A1 (en) * | 2002-06-14 | 2004-12-02 | Mccarthy Simon J, | Wound dressing and method for controlling severe, life-threatening bleeding |
US20080213344A1 (en) * | 2002-06-14 | 2008-09-04 | Providence Health System- Oregon | Wound dressing and method for controlling severe, life-threatening bleeding |
US8062669B2 (en) | 2002-06-20 | 2011-11-22 | Bioalliance Pharma | Vectorization system comprising nanoparticles of homogenous size of at least one polymer and at least one positively charged polysaccharide and method for the preparation thereof |
US20060134220A1 (en) * | 2002-06-20 | 2006-06-22 | Bioalliance Pharma | Vectorization system comprising nanoparticles of homogenous size of at least one polymer and at least one positively charged polysaccharide and method for the preparation thereof |
US20060058263A1 (en) * | 2002-11-01 | 2006-03-16 | Rogers Brent D | Heat pasturized liquids containing glucosamine |
US20060003965A1 (en) * | 2002-11-01 | 2006-01-05 | Fosdick Lawrence D | N-acetyl-d-glucosamine (nag) supplemented food products and beverages |
US20060246114A1 (en) * | 2002-11-01 | 2006-11-02 | Rogers Brent D | Multiple component food product useful for delivering glucosamine and/or nacetyl-d-glucosamine |
US8992453B2 (en) | 2003-09-12 | 2015-03-31 | Marine Polymer Technologies, Inc. | Vascular access preservation in hemodialysis patients |
US8951565B2 (en) | 2003-12-23 | 2015-02-10 | Hemcon Medical Technologies, Inc. | Hemostatic compositions, assemblies, systems, and methods employing particulate hemostatic agents formed from hydrophilic polymer foam such as chitosan |
US20090048210A1 (en) * | 2004-05-21 | 2009-02-19 | Saburo Minami | Agent for therapy or treatment of wound |
US20060211973A1 (en) * | 2005-03-17 | 2006-09-21 | Gregory Kenton W | Systems and methods for hemorrhage control and or tissue repair |
US9204957B2 (en) | 2005-03-17 | 2015-12-08 | Hemcon Medical Technologies, Inc. | Systems and methods for hemorrhage control and or tissue repair |
US20080287907A1 (en) * | 2006-05-23 | 2008-11-20 | Providence Health System-Oregan D/B/A Providence St. Vincent Medical Center | Systems and methods for introducing and applying a bandage structure within a body lumen or hollow body organ |
US8920514B2 (en) | 2006-05-23 | 2014-12-30 | Providence Health System—Oregon | Systems and methods for introducing and applying a bandage structure within a body lumen or hollow body organ |
US20090104284A1 (en) * | 2007-10-19 | 2009-04-23 | Gunn Jr Herbert Harrell | Topically Applied Chitosan Composition for Pain Relief and Boosting Immune System |
US20100172958A1 (en) * | 2008-05-02 | 2010-07-08 | Hemcon Medical Technologies, Inc. | Wound dressing devices and methods |
US9205170B2 (en) | 2008-05-02 | 2015-12-08 | Hemcon Medical Technologies, Inc. | Wound dressing devices and methods |
US10086105B2 (en) | 2008-10-06 | 2018-10-02 | Providence Health System—Oregon | Chitosan foam medical devices and methods |
US9533076B2 (en) | 2008-12-22 | 2017-01-03 | Abbott Laboratories | Carriers for hemostatic tract treatment |
US20100228185A1 (en) * | 2008-12-22 | 2010-09-09 | Abbott Laboratories | Carriers for hemostatic tract treatment |
US8517979B2 (en) | 2008-12-22 | 2013-08-27 | Abbott Laboratories | Carriers for hemostatic tract treatment |
WO2011026869A2 (en) | 2009-09-01 | 2011-03-10 | Medovent Gmbh | Tissue dressing kit |
EP3117842A1 (en) | 2009-09-01 | 2017-01-18 | Medovent GmbH | Tissue dressing material |
US8715719B2 (en) | 2010-06-16 | 2014-05-06 | Abbott Vascular, Inc. | Stable chitosan hemostatic implant and methods of manufacture |
WO2013109004A1 (en) | 2012-01-19 | 2013-07-25 | 주식회사 시지바이오 | Antimicrobial wound-covering material and method for manufacturing same |
US9610378B2 (en) | 2012-01-19 | 2017-04-04 | Cg Bio Co., Ltd. | Antimicrobial wound-covering material and method for manufacturing same |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US3632754A (en) | Use of chitin for promoting wound healing | |
US3911116A (en) | Process for promoting wound healing with chitin derivatives | |
US3914413A (en) | Process for facilitating wound healing with N-acetylated partially depolymerized chitin materials | |
US3903268A (en) | Chitin and chitin derivatives for promoting wound healing | |
JP3993633B2 (en) | Poly-β-1 → 4-N-acetylglucosamine | |
US6649599B2 (en) | Methods and compositions for poly-β-1-4-N-acetylglucosamine cell therapy system | |
EP1483299B1 (en) | Cell wall derivatives from biomass and preparation thereof | |
US20090247738A1 (en) | Biocompatible poly-beta-1-4-n-acetylglucosamine | |
CN106496357B (en) | A kind of O- quaternary ammonium salt-N- alkylated chitosan and the preparation method and application thereof | |
WO1996002260A1 (en) | Wound healing agent | |
US3624201A (en) | Compositions containing calcium and magnesium salts of citric, phosphoric and lactic acid and method of promoting healing of wounds therewith | |
US5624679A (en) | Methods and compositions for poly-β-1-4-N-acetylglucosamine biological barriers | |
CN113769150B (en) | Composite material with rapid blood coagulation effect and preparation method thereof | |
US20220378974A1 (en) | Hemostatic material and wound dressing containing same | |
CN110152055B (en) | Functional drug sustained-release medical dressing constructed by alginic acid aminated derivative/bacterial cellulose nanocrystalline composite gel | |
US3804949A (en) | Process of promoting healing of wounds | |
DE1906159A1 (en) | Chitin and egg-shell prepns for cicatrising wounds | |
Takamori et al. | Biodegradation of chitin with enzymes and vital components | |
Cabral et al. | Chitin-and chitosan-based strategies in wound healing | |
WO1990006124A1 (en) | Formulations for treating slow and non-healing wounds | |
CN110772660A (en) | Preparation method of guanidinated chitosan dressing | |
CN117659521A (en) | Extracellular polysaccharide-based hydrogel and preparation method and application thereof | |
CN116212095A (en) | Preparation method of molybdenum disulfide nanofiber membrane dressing | |
CA2372026A1 (en) | Poly-beta-1->4-n-acetylglucosamine | |
CA2604073A1 (en) | Poly-.beta.-1.fwdarw.4-n-acetylglucosamine |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AS | Assignment |
Owner name: LESCARDEN INC., Free format text: CHANGE OF NAME;ASSIGNOR:LESCARDEN LTD.;REEL/FRAME:004123/0510 Effective date: 19830105 |