CN104473706A - Biodegradable composite type tubular urethral stent and preparation method - Google Patents
Biodegradable composite type tubular urethral stent and preparation method Download PDFInfo
- Publication number
- CN104473706A CN104473706A CN201410750552.4A CN201410750552A CN104473706A CN 104473706 A CN104473706 A CN 104473706A CN 201410750552 A CN201410750552 A CN 201410750552A CN 104473706 A CN104473706 A CN 104473706A
- Authority
- CN
- China
- Prior art keywords
- thin film
- lactide
- poly
- internal layer
- composite tubular
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
The invention provides a biodegradable composite type tubular urethral stent and a preparation method. By the biodegradable composite type tubular urethral stent and the preparation method, the shortcomings that the using quantity of the existing patch materials is limited and complications are easily caused on sampled portions are overcome. The biodegradable tissue engineering stent comprises an inner layer and an outer layer, wherein the inner layer is tubular; the outer layer is arranged on the outer surface of the inner layer; the inner layer and the outer layer are made of degradable high polymer materials and acellular stromata; the degradable high polymer materials are polylactic acid- polylactic-co-glycolic acid (PLGA) films; the acellular stromata are small intestinal submucosa (SIS) films; and the polylactic acid- polylactic-co-glycolic acid (PLGA) films and the small intestinal submucosa (SIS) films are connected to one another. By the biodegradable composite type tubular urethral stent, autologous sampling is not required during urethral canal compounding and reestablishment, and the complications which are possibly caused on the sampled portions are avoided.
Description
Technical field
The present invention relates to medicine technology field, particularly a kind of degradable composite tubular urethra rack and preparation method.
Background technology
Traumatic or iatrogenic urethral injury, the urethral stricture that the gonococcal or various factors such as nonspecific urethritis and congenital urethra pathological changes causes is one of difficult problem of facing of urological surgeon.Conventional treatments comprises urethral dilatation, internal urethrotomy, end-to-end anastomosis of urethra etc. and is only applicable to the shorter urethral stricture patient of distance, patient for long section urethral stricture mainly adopts alternative operative treatment, and genital skin, bladder mucosa, albuginea penis, oral mucosa and lingual mucous membrane are all used as the patching material of urethra prosthesis; But draw materials and usually can cause the complication at position of drawing materials, and the amount of drawing materials is restricted.
Summary of the invention
The object of the invention is to cause for when existing and draw materials in existing long section urethral stricture Therapeutic Method the weak point that wound, material taken amount are limited, position of drawing materials easily leads to complications, a kind of degradable composite tubular urethra rack and preparation method are provided.
The present invention completes in the following way: a kind of degradable composite tubular urethra rack, this degradable composite tubular urethra rack is a kind of degradable tissue engineering bracket, comprise internal layer and skin, internal layer is tubulose, and skin is located on the outer surface of internal layer; Described internal layer and skin all adopt degradable macromolecular material and acellular matrix to make.
In the degradable composite tubular urethra rack of described one, described makes internal layer and outer field degradable high polymer material employing Poly(D,L-lactide-co-glycolide PLGA thin film, and described acellular matrix adopts small intestinal acellular matrix SIS thin film; Interconnect between Poly(D,L-lactide-co-glycolide PLGA thin film and small intestinal acellular matrix SIS thin film.
In the degradable composite tubular urethra rack of described one, pass through between described Poly(D,L-lactide-co-glycolide PLGA thin film with small intestinal acellular matrix SIS thin film together with suturing with thread management; Described Poly(D,L-lactide-co-glycolide PLGA thin film and small intestinal acellular matrix SIS thin film are all in strip structure; Described line is employing 9/0 nylon wire.
In the degradable composite tubular urethra rack of described one, described skin is wrapped on the outer surface of internal layer by spiral way.
In the degradable composite tubular urethra rack of described one, Poly(D,L-lactide-co-glycolide PLGA thin film on described internal layer and the placing flat of small intestinal acellular matrix SIS film water, longitudinal separation arrangement is stitched together in the form of sheets, around catheter external latasuture synthesis tubulose.
In the degradable composite tubular urethra rack of described one, the Poly(D,L-lactide-co-glycolide PLGA thin film on described skin and small intestinal acellular matrix SIS thin film level are intervally arranged and are stitched together in strip.
In the degradable composite tubular urethra rack of described one, described internal layer is arranged by the Poly(D,L-lactide-co-glycolide PLGA thin film of two rectangles and the small intestinal acellular matrix SIS thin film longitudinal separation of two rectangles and is formed; Described skin is intervally arranged by two rectangular-shaped Poly(D,L-lactide-co-glycolide PLGA thin film and two rectangular-shaped small intestinal acellular matrix SIS thin film levels and is stitched together.
The preparation method of a kind of degradable composite tubular urethra rack of the present invention comprises the following steps:
(1) macromolecular material polylactic acid PLA and polyglycolic acid PGA are pressed the mixed in molar ratio of 50:50 ~ 85:15, electrostatic spinning technique is adopted to obtain Poly(D,L-lactide-co-glycolide PLGA nanoscale electrospun fiber membrane, carry out modification in obtained electrospinning fibre film surface grafting I-type collagen, obtain modified Poly(D,L-lactide-co-glycolide PLGA thin film;
(2) small intestinal submucosa cell tissue is soaked in de-Cell sap, de-Cell sap by volumetric concentration be 0.2% Triton X-100-100 (Triton X-100) and volumetric concentration be 0.03% ammonia be mixed, vibration at-40 DEG C ~-50 DEG C temperature, carries out freezing after taking off cell and vacuum drying obtains small intestinal acellular matrix SIS thin film;
(3) according to the requirement of urethra caliber, the small intestinal acellular matrix SIS thin film Poly(D,L-lactide-co-glycolide PLGA thin film obtained by step (1) and step (2) obtained makes the rectangle of rule, obtains internal layer and skin with suturing with thread management.
In the preparation method of described a kind of degradable composite tubular urethra rack, described line adopts nylon wire.
In the preparation method of described a kind of degradable composite tubular urethra rack, the mol ratio of described polylactic acid PLA and polyglycolic acid PGA is 75:25.
In the preparation method of described a kind of degradable composite tubular urethra rack, the nanoscale electrospinning fibre film thickness of the Poly(D,L-lactide-co-glycolide PLGA that described step (1) is obtained is 0.35 ~ 0.45mm, porosity is 85% ~ 95%, Electrospun diameter is 300 ~ 450nm, and aperture is 60 ~ 100 μm.
In the preparation method of described a kind of degradable composite tubular urethra rack, the preparation method of described a kind of degradable composite tubular urethra rack adopts liquid phase cultural method on internal layer, inoculate the fat mesenchymal stem cell of VEGF (VEGF) 165 gene transfection, and skin is inoculated the smooth muscle cell transformed by fat mesenchymal stem cell.
The support microstructure of a kind of degradable composite tubular urethra rack of the present invention is three-dimensional porous shape, has good biocompatibility and mechanical property, has both elasticity and pliability, can keep stable tube-like condition in vivo; What the present invention adopted is polymeric biomaterial and acellular matrix, is conducive to seed cell and sticks, breeds and break up, and promotes the growth of surrounding tissue; Bi-material can progressively be decomposed into small-molecule substance by respective mode and naturally absorb in vivo, without the need to taking out.
Adopting the present invention to carry out urethra Reparation and Reconstruction, without the need to carrying out autologous drawing materials, avoiding the complication that position of drawing materials may cause; Mucous membrane of urethra epithelial cell, stem cell, smooth muscle cell etc. can adhere to and breed on support, can be directly used in urethra Reparation and Reconstruction, are applicable to clinical practice.
Accompanying drawing explanation
Figure l is the structural representation of a kind of degradable composite tubular urethra rack internal layer of the present invention.
Fig. 2 is the outer field structural representation of a kind of degradable composite tubular urethra rack of the present invention.
Fig. 3 is the expansion schematic diagram of Fig. 1.
Fig. 4 is the expansion schematic diagram of Fig. 2.
Fig. 5 is the Electronic Speculum figure of Poly(D,L-lactide-co-glycolide PLGA thin film.
Fig. 6 is the Electronic Speculum figure of small intestinal acellular matrix SIS thin film.
In accompanying drawing 1 ~ 6,1 represents internal layer; 2 represent outer; 3 represent Poly(D,L-lactide-co-glycolide PLGA thin film; 4 represent small intestinal acellular matrix SIS thin film.
Detailed description of the invention
Contrast accompanying drawing 1 ~ 6 below, by embodiment, the invention will be further described.
Embodiment 1
A preparation method for degradable composite tubular urethra rack, this preparation method comprises the following steps:
(1) by the ratio mixing of macromolecular material polylactic acid PLA and polyglycolic acid PGA 50:50 in molar ratio, electrostatic spinning technique is adopted to obtain Poly(D,L-lactide-co-glycolide PLGA nanoscale electrospun fiber membrane, carry out modification in obtained electrospinning fibre film surface grafting I-type collagen, obtain modified Poly(D,L-lactide-co-glycolide PLGA thin film;
(2) submucous layer of small intestine cell tissue is soaked in by volumetric concentration be 0.2% Triton X-100-100 (Triton X-100) and volumetric concentration be in the de-Cell sap that is mixed of ammonia of 0.03%, at-40 DEG C of temperature, carry out freezing and vacuum drying treatment after the de-cell of vibration, obtain small intestinal acellular matrix SIS thin film;
(3) according to the requirement of urethra caliber, the small intestinal acellular matrix SIS thin film Poly(D,L-lactide-co-glycolide PLGA thin film obtained by step (1) and step (2) obtained makes the rectangle of rule, obtains internal layer 1 and outer 2 with 9/0 nylon line suture.
The groin fat of aseptic taking-up new zealand white rabbit, collagenase method isolates rabbit fat mesenchymal stem cell (ADMSCs), and carries out stationary culture In vitro culture; Adopt the method for slow-virus transfection to build process LAN VEGF (VEGF) 165 to the fat mesenchymal stem cell (ADMSCs) after cultivating, obtain the fat mesenchymal stem cell of VEGF (VEGF) 165 gene transfection; Fat mesenchymal stem cell (ADMSCs) after cultivation is induced 2 weeks under platelet derived growth factor (PDGF-BB) conventional culture conditions of 20ng/ml, is divided into smooth muscle cell; Adopt liquid phase cultural method on the internal layer 1 obtained by above-mentioned preparation method, inoculate the fat mesenchymal stem cell of VEGF (VEGF) 165 gene transfection, on outer 2, inoculate the smooth muscle cell transformed by fat mesenchymal stem cell; Outer 2 are located on the lateral surface of internal layer 1; Described internal layer 1 is tubular structure, by two length be 30mm, width is that the OBL Poly(D,L-lactide-co-glycolide PLGA thin film of 3mm and two length are 30mm, width is that 3mm OBL small intestinal acellular matrix SIS thin film longitudinal separation is arranged and is stitched together in the form of sheets, around catheter external latasuture synthesis tubulose; Outer 2 by two length be 30mm, width is that 3mm OBL Poly(D,L-lactide-co-glycolide PLGA and two length is 30mm, width is arrange and sew up and make strip in 3mm OBL small intestinal acellular matrix SIS horizontal interval, skin 2 is spirally wound on the outer surface of internal layer 1.
Fig. 5 is the Electronic Speculum figure of the Poly(D,L-lactide-co-glycolide PLGA thin film obtained; Fig. 6 is the Electronic Speculum figure of the small intestinal acellular matrix SIS thin film obtained.
Embodiment 2
A preparation method for degradable composite tubular urethra rack, this preparation method comprises the following steps:
(1) by the ratio mixing of macromolecular material polylactic acid PLA and polyglycolic acid PGA 75:25 in molar ratio, electrostatic spinning technique is adopted to obtain Poly(D,L-lactide-co-glycolide PLGA nanoscale electrospun fiber membrane, thickness is 0.4mm, porosity is 90%, Electrospun diameter is 300-450nm, and aperture is 60-100 μm; Carry out modification in obtained electrospinning fibre film surface grafting I-type collagen, obtain modified Poly(D,L-lactide-co-glycolide PLGA thin film;
(2) submucous layer of small intestine cell tissue is soaked in by volumetric concentration be 0.2% Triton X-100-100 (Triton X-100) and volumetric concentration be in the de-Cell sap that is mixed of ammonia of 0.03%, at-42 DEG C of temperature, carry out freezing process and vacuum drying treatment after the de-cell of vibration, obtain small intestinal acellular matrix SIS thin film;
(3) according to the requirement of urethra caliber, the small intestinal acellular matrix SIS thin film Poly(D,L-lactide-co-glycolide PLGA thin film obtained by step (1) and step (2) obtained is cut into the rectangle of rule, obtains internal layer 1 and outer 2 with 9/0 nylon line suture.
The groin fat of aseptic taking-up new zealand white rabbit, collagenase method isolates rabbit fat mesenchymal stem cell (ADMSCs), and carries out stationary culture In vitro culture; Adopt the method for slow-virus transfection to build process LAN VEGF (VEGF) 165 to the fat mesenchymal stem cell (ADMSCs) after cultivating, obtain the fat mesenchymal stem cell of VEGF (VEGF) 165 gene transfection; Fat mesenchymal stem cell (ADMSCs) after cultivation is induced 2 weeks under platelet derived growth factor (PDGF-BB) conventional culture conditions of 20ng/ml, is divided into smooth muscle cell; Adopt liquid phase cultural method on the internal layer 1 obtained by above-mentioned preparation method, inoculate the fat mesenchymal stem cell of VEGF (VEGF) 165 gene transfection, on outer 2, inoculate the smooth muscle cell transformed by fat mesenchymal stem cell; Outer 2 are located on the lateral surface of internal layer 1; Described internal layer 1 is tubular structure, by two length be 30mm, width is that the OBL Poly(D,L-lactide-co-glycolide PLGA thin film of 3mm and two length are 30mm, width is that 3mm OBL small intestinal acellular matrix SIS thin film longitudinal separation is arranged and is stitched together in the form of sheets, around catheter external latasuture synthesis tubulose; Outer 2 by two length be 30mm, width is that 3mm OBL Poly(D,L-lactide-co-glycolide PLGA and two length is 30mm, width is arrange and sew up and make strip in 3mm OBL small intestinal acellular matrix SIS horizontal interval, skin 2 is spirally wound on the outer surface of internal layer 1.
Embodiment 3
A preparation method for degradable composite tubular urethra rack, this preparation method comprises the following steps:
(1) by the ratio mixing of macromolecular material polylactic acid PLA and polyglycolic acid PGA 85:15 in molar ratio, electrostatic spinning technique is adopted to obtain Poly(D,L-lactide-co-glycolide PLGA nanoscale electrospun fiber membrane, carry out modification in obtained electrospinning fibre film surface grafting I-type collagen, obtain modified Poly(D,L-lactide-co-glycolide PLGA thin film;
(2) submucous layer of small intestine cell tissue is soaked in by volumetric concentration be 0.2% Triton X-100-100 (Triton X-100) and volumetric concentration be in the de-Cell sap that is mixed of ammonia of 0.03%, at-50 DEG C of temperature, carry out freezing and vacuum drying treatment after the de-cell of vibration, obtain small intestinal acellular matrix SIS thin film;
(3) according to the requirement of urethra caliber, the small intestinal acellular matrix SIS thin film Poly(D,L-lactide-co-glycolide PLGA thin film obtained by step (1) and step (2) obtained is cut into the rectangle of rule, obtains internal layer 1 and outer 2 with 9/0 nylon line suture.
The groin fat of aseptic taking-up new zealand white rabbit, collagenase method isolates rabbit fat mesenchymal stem cell (ADMSCs), and carries out stationary culture In vitro culture; Adopt the method for slow-virus transfection to build process LAN VEGF (VEGF) 165 to the fat mesenchymal stem cell (ADMSCs) after cultivating, obtain the fat mesenchymal stem cell of VEGF (VEGF) 165 gene transfection; Fat mesenchymal stem cell (ADMSCs) after cultivation is induced 2 weeks under platelet derived growth factor (PDGF-BB) conventional culture conditions of 20ng/ml, is divided into smooth muscle cell; Adopt liquid phase cultural method on the internal layer 1 obtained by above-mentioned preparation method, inoculate the fat mesenchymal stem cell of VEGF (VEGF) 165 gene transfection, on outer 2, inoculate the smooth muscle cell transformed by fat mesenchymal stem cell; Outer 2 are located on the lateral surface of internal layer 1; Described internal layer 1 is tubular structure, by two length be 30mm, width is that the OBL Poly(D,L-lactide-co-glycolide PLGA thin film of 3mm and two length are 30mm, width is that 3mm OBL small intestinal acellular matrix SIS thin film longitudinal separation is arranged and is stitched together in the form of sheets, around catheter external latasuture synthesis tubulose; Outer 2 by two length be 30mm, width is that 3mm OBL Poly(D,L-lactide-co-glycolide PLGA and two length is 30mm, width is arrange and sew up and make strip in 3mm OBL small intestinal acellular matrix SIS horizontal interval, skin 2 is spirally wound on the outer surface of internal layer 1.
Claims (10)
1. a degradable composite tubular urethra rack, it is characterized in that this degradable composite tubular urethra rack is a kind of degradable tissue engineering bracket, comprise internal layer and skin, internal layer is tubulose, and skin is located on the outer surface of internal layer; Described internal layer and skin all adopt degradable macromolecular material and acellular matrix to make.
2. the degradable composite tubular urethra rack of one according to claim 1, it is characterized in that described making internal layer and outer field degradable high polymer material adopts Poly(D,L-lactide-co-glycolide PLGA thin film, described acellular matrix adopts small intestinal acellular matrix SIS thin film; Interconnect between Poly(D,L-lactide-co-glycolide PLGA thin film and small intestinal acellular matrix SIS thin film.
3. the degradable composite tubular urethra rack of one according to claim 1 and 2, is characterized in that described skin 2 is wrapped on the outer surface of internal layer by spiral way.
4. the degradable composite tubular urethra rack of one according to claim 2, is characterized in that between described Poly(D,L-lactide-co-glycolide PLGA thin film with small intestinal acellular matrix SIS thin film by together with suturing with thread management; Described Poly(D,L-lactide-co-glycolide PLGA thin film and small intestinal acellular matrix SIS thin film are all in strip structure.
5. the degradable composite tubular urethra rack of one according to claim 4, it is characterized in that the Poly(D,L-lactide-co-glycolide PLGA thin film on described internal layer and the placing flat of small intestinal acellular matrix SIS film water, longitudinal separation arrangement is stitched together in the form of sheets, around catheter external latasuture synthesis tubulose.
6. the degradable composite tubular urethra rack of one according to claim 4, is characterized in that Poly(D,L-lactide-co-glycolide PLGA thin film on described skin and small intestinal acellular matrix SIS thin film level are intervally arranged and are stitched together in strip.
7. the preparation method of a kind of degradable composite tubular urethra rack according to any one of claim 1 to 6, is characterized in that this preparation method comprises the following steps:
(1) macromolecular material polylactic acid PLA and polyglycolic acid PGA are pressed the mixed in molar ratio of 50:50 ~ 85:15, electrostatic spinning technique is adopted to obtain Poly(D,L-lactide-co-glycolide PLGA nanoscale electrospun fiber membrane, carry out modification in obtained electrospinning fibre film surface grafting I-type collagen, obtain modified Poly(D,L-lactide-co-glycolide PLGA thin film;
(2) submucous layer of small intestine cell tissue is soaked in by volumetric concentration be 0.2% Triton X-100-100 (Triton X-100) and volumetric concentration be in the de-Cell sap that is mixed of ammonia of 0.03%, at-40 DEG C ~-50 DEG C temperature, carry out freezing and vacuum drying treatment after the de-cell of vibration, obtain small intestinal acellular matrix SIS thin film;
(3) according to the requirement of urethra caliber, the small intestinal acellular matrix SIS thin film Poly(D,L-lactide-co-glycolide PLGA thin film obtained by step (1) and step (2) obtained makes the rectangle of rule, obtains internal layer and skin with suturing with thread management.
8. the preparation method of a kind of degradable composite tubular urethra rack according to claim 7, is characterized in that described line adopts nylon wire.
9. the preparation method of a kind of degradable composite tubular urethra rack according to claim 7 or 8, is characterized in that the mol ratio of described polylactic acid PLA and polyglycolic acid PGA is 75:25.
10. the preparation method of a kind of degradable composite tubular urethra rack according to claim 7 or 8, it is characterized in that adopting liquid phase cultural method on internal layer, inoculate the fat mesenchymal stem cell of VEGF (VEGF) 165 gene transfection, skin is inoculated the smooth muscle cell transformed by fat mesenchymal stem cell.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410750552.4A CN104473706B (en) | 2014-12-09 | 2014-12-09 | Biodegradable composite type tubular urethral stent and preparation method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410750552.4A CN104473706B (en) | 2014-12-09 | 2014-12-09 | Biodegradable composite type tubular urethral stent and preparation method |
Publications (2)
Publication Number | Publication Date |
---|---|
CN104473706A true CN104473706A (en) | 2015-04-01 |
CN104473706B CN104473706B (en) | 2017-02-22 |
Family
ID=52748413
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201410750552.4A Expired - Fee Related CN104473706B (en) | 2014-12-09 | 2014-12-09 | Biodegradable composite type tubular urethral stent and preparation method |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN104473706B (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107596447A (en) * | 2017-10-11 | 2018-01-19 | 广州新诚生物科技有限公司 | A kind of biological medicinal membrane with tissue repair function and preparation method thereof |
CN108853579A (en) * | 2018-07-27 | 2018-11-23 | 吉林大学 | A kind of preparation method of artificial tubular biomaterial |
CN110680961A (en) * | 2019-11-12 | 2020-01-14 | 东南大学 | Preparation method of multilayer spiral urethral tissue engineering scaffold |
CN113244021A (en) * | 2021-05-12 | 2021-08-13 | 太原理工大学 | Support for preventing external auditory canal stenosis through 3D printing acellular matrix and preparation method |
Citations (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0634152A1 (en) * | 1993-06-15 | 1995-01-18 | Esa Viherkoski | Biodegradable urethal stent |
WO2004110315A1 (en) * | 2003-06-16 | 2004-12-23 | Nanyang Technological University | Polymeric stent and method of manufacture |
CN101433735A (en) * | 2007-11-13 | 2009-05-20 | 北京大清生物技术有限公司 | Method for preparing SIS tissue repair material |
CN101810883A (en) * | 2009-12-25 | 2010-08-25 | 上海太亨科贸有限公司 | Bio-derived material with high tissue compatibility and long acting anti-infection as well as preparation method and application thereof |
CN101987050A (en) * | 2009-08-06 | 2011-03-23 | 浙江普洛医药科技有限公司 | Degradable double-layer compound ureteral stent tube |
CN102274491A (en) * | 2011-07-13 | 2011-12-14 | 中国人民解放军第四军医大学 | Stem cell combination growth factor injection for promoting angiogenesis of ischemic tissue, and its preparation method and application method |
CN102475910A (en) * | 2010-11-22 | 2012-05-30 | 大连创达技术交易市场有限公司 | Esophageal tissue constructed by tissue engineering |
CN102488926A (en) * | 2011-12-16 | 2012-06-13 | 东华大学 | Tissue engineering scaffold for urethra reconstruction and preparation method thereof |
CN102895702A (en) * | 2012-11-09 | 2013-01-30 | 四川大学华西医院 | Composite manual bile duct and preparation method thereof |
CN103877622A (en) * | 2014-03-26 | 2014-06-25 | 中山大学 | Electrostatic spinning nanofiber-extracellular matrix composite material as well as preparation method and application thereof |
CN204618494U (en) * | 2014-12-09 | 2015-09-09 | 金华市人民医院 | A kind of degradable composite tubular urethra rack |
-
2014
- 2014-12-09 CN CN201410750552.4A patent/CN104473706B/en not_active Expired - Fee Related
Patent Citations (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0634152A1 (en) * | 1993-06-15 | 1995-01-18 | Esa Viherkoski | Biodegradable urethal stent |
WO2004110315A1 (en) * | 2003-06-16 | 2004-12-23 | Nanyang Technological University | Polymeric stent and method of manufacture |
CN1812754A (en) * | 2003-06-16 | 2006-08-02 | 南洋理工大学 | Polymeric stent and method of manufacture |
CN101433735A (en) * | 2007-11-13 | 2009-05-20 | 北京大清生物技术有限公司 | Method for preparing SIS tissue repair material |
CN101987050A (en) * | 2009-08-06 | 2011-03-23 | 浙江普洛医药科技有限公司 | Degradable double-layer compound ureteral stent tube |
CN101810883A (en) * | 2009-12-25 | 2010-08-25 | 上海太亨科贸有限公司 | Bio-derived material with high tissue compatibility and long acting anti-infection as well as preparation method and application thereof |
CN102475910A (en) * | 2010-11-22 | 2012-05-30 | 大连创达技术交易市场有限公司 | Esophageal tissue constructed by tissue engineering |
CN102274491A (en) * | 2011-07-13 | 2011-12-14 | 中国人民解放军第四军医大学 | Stem cell combination growth factor injection for promoting angiogenesis of ischemic tissue, and its preparation method and application method |
CN102488926A (en) * | 2011-12-16 | 2012-06-13 | 东华大学 | Tissue engineering scaffold for urethra reconstruction and preparation method thereof |
CN102895702A (en) * | 2012-11-09 | 2013-01-30 | 四川大学华西医院 | Composite manual bile duct and preparation method thereof |
CN103877622A (en) * | 2014-03-26 | 2014-06-25 | 中山大学 | Electrostatic spinning nanofiber-extracellular matrix composite material as well as preparation method and application thereof |
CN204618494U (en) * | 2014-12-09 | 2015-09-09 | 金华市人民医院 | A kind of degradable composite tubular urethra rack |
Non-Patent Citations (4)
Title |
---|
吕向国,徐月敏: "组织工程技术在尿道重建中的应用", 《中国组织工程研究》 * |
姜华,王忠: "脱细胞基质在尿道组织工程中的应用", 《国际泌尿系统杂》 * |
杨凯,张育敏,张乃丽,徐伟俊,李宝兴: "小肠黏膜下层在组织修复重建中的应用研究进展", 《中国修复重建外科杂志》 * |
赵阳,张明,王忠,卢慕峻: "组织工程支架材料在膀胱修复中的应用进展", 《组织工程与重建外科杂志》 * |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107596447A (en) * | 2017-10-11 | 2018-01-19 | 广州新诚生物科技有限公司 | A kind of biological medicinal membrane with tissue repair function and preparation method thereof |
CN108853579A (en) * | 2018-07-27 | 2018-11-23 | 吉林大学 | A kind of preparation method of artificial tubular biomaterial |
CN110680961A (en) * | 2019-11-12 | 2020-01-14 | 东南大学 | Preparation method of multilayer spiral urethral tissue engineering scaffold |
CN113244021A (en) * | 2021-05-12 | 2021-08-13 | 太原理工大学 | Support for preventing external auditory canal stenosis through 3D printing acellular matrix and preparation method |
Also Published As
Publication number | Publication date |
---|---|
CN104473706B (en) | 2017-02-22 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US20230061170A1 (en) | Fiber scaffolds for use creating implantable structures | |
US11850141B2 (en) | Bioerodible wraps and uses therefor | |
US20190153398A1 (en) | Fiber scaffolds for use in esophageal prostheses | |
CN102784015B (en) | Artificial blood vessel loaded with pseudo-ginseng medicines, and preparation method and application for artificial blood vessel | |
US8388692B2 (en) | Thin film multilocular structure made of collagen, member for tissue regeneration containing the same, and method for producing the same | |
Chung et al. | Development of an omentum-cultured oesophageal scaffold reinforced by a 3D-printed ring: feasibility of an in vivo bioreactor | |
US10092679B2 (en) | Laminous vascular constructs combining cell sheet engineering and electrospinning technologies | |
CN104473706B (en) | Biodegradable composite type tubular urethral stent and preparation method | |
CN108495599A (en) | Systems and methods for creating gastrointestinal tract tissue at an anastomosis site or other physiological site | |
CA2640601A1 (en) | Biomimetic fibrous polymer scaffolds | |
Kim et al. | Tissue-engineered esophagus via bioreactor cultivation for circumferential esophageal reconstruction | |
Guang et al. | Design and preparation of polyurethane-collagen/heparin-conjugated polycaprolactone double-layer bionic small-diameter vascular graft and its preliminary animal tests | |
Hoogenkamp et al. | Seamless vascularized large-diameter tubular collagen scaffolds reinforced with polymer knittings for esophageal regenerative medicine | |
JP2015513349A (en) | Basic material system for tissue repair | |
JP4168740B2 (en) | Collagen artificial blood vessel | |
WO2009125196A1 (en) | Neural circuits and stereotactic apparatus | |
CN204618494U (en) | A kind of degradable composite tubular urethra rack | |
US20230310002A1 (en) | Bridging peripheral nerve gaps with conduits for enhanced nerve regeneration | |
Nazari et al. | Advancing standard techniques for treatment of perianal fistula; when tissue engineering meets seton | |
JP2017519565A (en) | in situ tissue engineering | |
CN204219093U (en) | A kind of degradable spherical faux bladder | |
KR102347096B1 (en) | Artificial esophagus scaffold and manufacturing method thereof | |
WO2019151338A1 (en) | Tubular artificial organ | |
CN104188734B (en) | The spherical artificial urinary bladder of a kind of degradable | |
JP2020120944A (en) | Tubular reinforcement body and tubular artificial organ |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
CF01 | Termination of patent right due to non-payment of annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170222 Termination date: 20181209 |