CN103976805A - Fabrication method for hydrogel/macromolecule polymer film muscular tissue support - Google Patents

Fabrication method for hydrogel/macromolecule polymer film muscular tissue support Download PDF

Info

Publication number
CN103976805A
CN103976805A CN201410235459.XA CN201410235459A CN103976805A CN 103976805 A CN103976805 A CN 103976805A CN 201410235459 A CN201410235459 A CN 201410235459A CN 103976805 A CN103976805 A CN 103976805A
Authority
CN
China
Prior art keywords
solution
silica gel
hydrogel
high molecular
muscular tissue
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201410235459.XA
Other languages
Chinese (zh)
Other versions
CN103976805B (en
Inventor
连芩
陈成
李涤尘
秦霆
贺健康
李志朝
刘亚雄
靳忠民
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Xian Jiaotong University
Original Assignee
Xian Jiaotong University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Xian Jiaotong University filed Critical Xian Jiaotong University
Priority to CN201410235459.XA priority Critical patent/CN103976805B/en
Publication of CN103976805A publication Critical patent/CN103976805A/en
Application granted granted Critical
Publication of CN103976805B publication Critical patent/CN103976805B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Abstract

The invention discloses a fabrication method for a hydrogel/macromolecule polymer film muscular tissue support. The method comprises obtaining a three-dimensional support structure through a layer-upon-layer stacking mode; designing and fabricating a blood vessel network structure mold and a directional flow passage mold according to muscle structural characteristics; fabricating polymer directional film through an electro-spinning technology, fabricating a support single-layer plane structure, stacking other layers of supports on the three-dimensional support structure, and performing covering with the macromolecule polymer film support bonded on the outermost layer to obtain the hydrogel/macromolecule polymer film support. According to the method, the blood vessel network and the directional flow passage are fabricated on hydrogel, directional growth of cells can be achieved through the directional flow passage, nutrition and oxygen supply can be achieved through blood vessel network pipes, the polymer film covered on the periphery has a directional structure, and accordingly, directional growth of peripheral cells can be achieved, stretched film is bound and sewn, and fixation of the support is achieved.

Description

The manufacture method of hydrogel/high molecular polymer thin film muscular tissue support
Technical field
The present invention relates to a kind of preparation method of muscular tissue support, be specifically related to a kind of manufacture method of hydrogel/high molecular polymer thin film muscular tissue support.
Background technology
Muscle comprises cardiac muscle, smooth muscle, skeletal muscle, and three's identical point is to have sarcostyle, blood vessel and nerve, and myocyte becomes to align.Difference is distribution, the form of tissue in three, the difference to some extent such as sarcostyle quantity form, function.Skeletal muscle is relatively large soft tissue in human body, accounts for 40%~45% of body weight, and it is connected with bone photo by tendon, when receiving stimulation, by contraction and the diastole of skeletal muscle, realizes the function of body movement.Normal muscle regeneration is mainly to be completed by muscle satellite cell (satellite cell, SC), but SC quantity is few, and declines with age growth.For muscle injury serious or that area is larger, SC is not enough to reach the object of reparation, and wound surface is finally replaced by fibrous tissue and forms cicatrix, causes wound difficult, and body loses motor function.At present for large-area muscle injury, the most general treatment means is that musculo cutaneous flap is transplanted clinically, the damage of the method Dui Gong district is larger, and it is limited to originate, muscular tissue support provides possibility for overcoming these shortcomings, but the preparation process of existing muscular tissue support is more difficult, with the three-dimensional structure difficulty of muscular tissue support of blood vessel structure, and muscular tissue support is fixing inconvenient.
Summary of the invention
The object of the invention is to overcome the shortcoming of above-mentioned prior art, a kind of manufacture method of hydrogel/high molecular polymer thin film muscular tissue support is provided, the method is convenient to the three-dimensional structure of muscular tissue support of blood vessel structure and is fixed.
For achieving the above object, the manufacture method of hydrogel/high molecular polymer thin film muscular tissue support of the present invention comprises the following steps:
1) the inner tubular structure model of drafting hydrogel/high molecular polymer thin film muscular tissue support, then machine-processed for N resin piece by photocureable rapid shaping according to described inner tubular structure model;
2) prepare silicon sol solution, then by step 1) N resin piece obtaining be separately fixed on silica gel mould, then by the silica gel solution Implanted Silicon rubber moulding preparing, and then remove the bubble in silica gel solution by evacuation, leave standstill curing and demolding, obtain N shell silica gel mould;
3) preparation high molecular polymer PLGA solution, then carries out electrostatic spinning to the high molecular polymer PLGA solution preparing, and obtains thin polymer film;
4) to step 2) must carry out hydrophilic treated by N shell silica gel mould;
5) preparation gelatin solution, then passes through evacuation de-soak by the gelatin solution preparing;
6) preparation glutamine transaminage solution, then glutamine transaminage solution is divided into N part, simultaneously by step 5) gelatin solution after de-soak that obtains is divided into N part, by first part of glutamine transaminage solution and first part of gelatin solution mix homogeneously after de-soak, obtain first part of mixed solution A, then first part of mixed solution A is injected in ground floor silica gel mould, and by step 3) thin polymer film obtaining is bonded in the bottom surface of ground floor silica gel mould, make the gel phase of thin polymer film and first part of mixed solution A formation bonding, and then through solidifying and the demoulding, obtain support monolayer planar structure,
7) by second part of glutamine transaminage solution and second part of gelatin solution mix homogeneously after de-soak, obtain second part of mixed solution A, then second part of mixed solution A is injected in second layer silica gel mould, and by bonding the bottom of the gel of the top of support monolayer planar structure and second part of mixed solution A formation, and then carry out the demoulding;
8) the gelatin solution mix homogeneously after de-soak by X part glutamine transaminage solution and X part, obtain X part mixed solution A, then X part mixed solution A is injected in X layer silica gel mould, and the bottom of the gel of the top of the gel that X-1 part mixed solution A is formed and the formation of X part mixed solution A is bonding, and then carry out the demoulding, wherein, 3≤X≤N;
9) repeating step 8), obtain 3-D solid structure, then by step 3) thin polymer film obtaining is wrapping to the surface of 3-D solid structure, after insulation, obtains hydrogel/high molecular polymer thin film muscular tissue support.
Step 2) in the concrete steps of prepare silicon sol solution be: get silica gel and the firming agent of certain volume, then firming agent is added in silica gel, obtain silica gel solution, wherein, the volume ratio of silica gel and firming agent is 100: 1.5-2;
Step 2) in resin piece be fixed on silica gel mould by double faced adhesive tape, place 24-28h solidify.
Step 3) in solvent in PLGA macromolecule polymer solution be deionized water, the mass concentration of PLGA macromolecule polymer solution is 15~20%;
Step 3) in the high molecular polymer PLGA solution preparing is carried out to voltage in the process of electrostatic spinning is 10-15kV, flow velocity is 0.1~0.3ml/min, roller rotating speed is 2800~3600r/min.
Step 4) in described N shell silica gel mould is carried out to hydrophilic treated concrete operations be: described N shell silica gel mould is placed into plasma cleaner ionic medium and cleans 2~5min.
Step 5) in preparation gelatin solution detailed process be: take a certain amount of gelatin, gelatin is poured in the phosphate buffer of certain volume, be to melt 30-40min under the condition of 60-70 DEG C in temperature, stir, obtain gelatin solution, in gelatin solution, the mass concentration of gelatin is 10%~20%;
In mixed solution A, the mass concentration of glutamine transaminage is 1~1.2%;
Step 6) in curing hardening time be 4~6min;
Step 6) in step 3) to pass in and out direction consistent for orientation direction and the blood vessel of the thin polymer film that obtains.
The time of each part of mixed solution A formation gel is 4~6min.
Step 9) in insulation concrete operations be: 3-D solid structure is placed in 37 DEG C of calorstats and is incubated 4~5h.
The present invention has following beneficial effect:
The manufacture method of hydrogel/high molecular polymer thin film muscular tissue support of the present invention is in preparation process, first prepare N shell silica gel mould, again described N shell silica gel mould is obtained to 3-D solid structure by the method being layering, then thin polymer film is wrapped on described 3-D solid structure, thereby obtain hydrogel/high molecular polymer thin film muscular tissue support, preparation process is simple, easy operating.The three-dimensional that the present invention adopts the method being layering to realize hydrogel/high molecular polymer film frame builds, hydrogel/high molecular polymer thin film muscular tissue support of preparation has blood vessel network structure and directive construction, blood vessel network can provide nutrition and oxygen for the cell in hydrogel/high molecular polymer film frame, directive construction can be realized the oriented growth of cell, thin polymer film is wrapped up to described 3-D solid structure, thin polymer film self has directive construction on the one hand, for cell directional growth provides topological structure, the peripheral parcel of thin polymer film can increase the intensity of hydrogel on the other hand, can realize hydrogel/high molecular polymer film frame fixed function simultaneously, thereby the three-dimensional blood vessel network that has solved muscular tissue support builds, cell directional growth and the fixing problem of support.
Brief description of the drawings
Fig. 1 is the structural representation of hydrogel/high molecular polymer thin film muscular tissue support of preparing of the present invention.
Detailed description of the invention
Below in conjunction with accompanying drawing, the present invention is described in further detail:
With reference to figure 1, the manufacture method of hydrogel/high molecular polymer thin film muscular tissue support of the present invention comprises the following steps:
1) the inner tubular structure model of drafting hydrogel/high molecular polymer thin film muscular tissue support, then machine-processed for N resin piece by photocureable rapid shaping according to described inner tubular structure model;
2) prepare silicon sol solution, then by step 1) N resin piece obtaining be separately fixed on silica gel mould, then by the silica gel solution Implanted Silicon rubber moulding preparing, and then remove the bubble in silica gel solution by evacuation, leave standstill curing and demolding, obtain N shell silica gel mould;
3) preparation high molecular polymer PLGA solution, then carries out electrostatic spinning to the high molecular polymer PLGA solution preparing, and obtains thin polymer film;
4) to step 2) must carry out hydrophilic treated by N shell silica gel mould;
5) preparation gelatin solution, then passes through evacuation de-soak by the gelatin solution preparing;
6) preparation glutamine transaminage solution, then glutamine transaminage solution is divided into N part, simultaneously by step 5) gelatin solution after de-soak that obtains is divided into N part, by first part of glutamine transaminage solution and first part of gelatin solution mix homogeneously after de-soak, obtain first part of mixed solution A, then first part of mixed solution A is injected in ground floor silica gel mould, and by step 3) thin polymer film obtaining is bonded in the bottom surface of ground floor silica gel mould, make the gel phase of thin polymer film and first part of mixed solution A formation bonding, and then through solidifying and the demoulding, obtain support monolayer planar structure,
7) by second part of glutamine transaminage solution and second part of gelatin solution mix homogeneously after de-soak, obtain second part of mixed solution A, then second part of mixed solution A is injected in second layer silica gel mould, and by bonding the bottom of the gel of the top of support monolayer planar structure and second part of mixed solution A formation, and then carry out the demoulding;
8) the gelatin solution mix homogeneously after de-soak by X part glutamine transaminage solution and X part, obtain X part mixed solution A, then X part mixed solution A is injected in X layer silica gel mould, and the bottom of the gel of the top of the gel that X-1 part mixed solution A is formed and the formation of X part mixed solution A is bonding, and then carry out the demoulding, wherein, 3≤X≤N;
9) repeating step 8), obtain 3-D solid structure, then by step 3) thin polymer film obtaining is wrapping to the surface of 3-D solid structure, after insulation, obtains hydrogel/high molecular polymer thin film muscular tissue support.
Step 2) in the concrete steps of prepare silicon sol solution be: get silica gel and the firming agent of certain volume, then firming agent is added in silica gel, obtain silica gel solution, wherein, the volume ratio of silica gel and firming agent is 100: 1.5-2;
Step 2) in resin piece be fixed on silica gel mould by double faced adhesive tape, place 24-28h solidify.
Step 3) in solvent in PLGA macromolecule polymer solution be deionized water, the mass concentration of PLGA macromolecule polymer solution is 15~20%;
Step 3) in the high molecular polymer PLGA solution preparing is carried out to voltage in the process of electrostatic spinning is 10-15kV, flow velocity is 0.1~0.3ml/min, roller rotating speed is 2800~3600r/min.
Step 4) in described N shell silica gel mould is carried out to hydrophilic treated concrete operations be: described N shell silica gel mould is placed into plasma cleaner ionic medium and cleans 2~5min.
Step 5) in preparation gelatin solution detailed process be: take a certain amount of gelatin, gelatin is poured in the phosphate buffer of certain volume, be to melt 30-40min under the condition of 60-70 DEG C in temperature, stir, obtain gelatin solution, in gelatin solution, the mass concentration of gelatin is 10%~20%;
In mixed solution A, the mass concentration of glutamine transaminage is 1~1.2%;
Step 6) in curing hardening time be 4~6min;
Step 6) in step 3) to pass in and out direction consistent for orientation direction and the blood vessel of the thin polymer film that obtains.
The time of each part of mixed solution A formation gel is 4~6min.
Step 9) in insulation concrete operations be: 3-D solid structure is placed in 37 DEG C of calorstats and is incubated 4~5h.

Claims (8)

1. a manufacture method for hydrogel/high molecular polymer thin film muscular tissue support, is characterized in that, comprises the following steps:
1) the inner tubular structure model of drafting hydrogel/high molecular polymer thin film muscular tissue support, then machine-processed for N resin piece by photocureable rapid shaping according to described inner tubular structure model;
2) prepare silicon sol solution, then by step 1) N resin piece obtaining be separately fixed on silica gel mould, then by the silica gel solution Implanted Silicon rubber moulding preparing, and then remove the bubble in silica gel solution by evacuation, leave standstill curing and demolding, obtain N shell silica gel mould;
3) preparation high molecular polymer PLGA solution, then carries out electrostatic spinning to the high molecular polymer PLGA solution preparing, and obtains thin polymer film;
4) to step 2) must carry out hydrophilic treated by N shell silica gel mould;
5) preparation gelatin solution, then passes through evacuation de-soak by the gelatin solution preparing;
6) preparation glutamine transaminage solution, then glutamine transaminage solution is divided into N part, simultaneously by step 5) gelatin solution after de-soak that obtains is divided into N part, by first part of glutamine transaminage solution and first part of gelatin solution mix homogeneously after de-soak, obtain first part of mixed solution A, then first part of mixed solution A is injected in ground floor silica gel mould, and by step 3) thin polymer film obtaining is bonded in the bottom surface of ground floor silica gel mould, make the gel phase of thin polymer film and first part of mixed solution A formation bonding, and then through solidifying and the demoulding, obtain support monolayer planar structure,
7) by second part of glutamine transaminage solution and second part of gelatin solution mix homogeneously after de-soak, obtain second part of mixed solution A, then second part of mixed solution A is injected in second layer silica gel mould, and by bonding the bottom of the gel of the top of support monolayer planar structure and second part of mixed solution A formation, and then carry out the demoulding;
8) the gelatin solution mix homogeneously after de-soak by X part glutamine transaminage solution and X part, obtain X part mixed solution A, then X part mixed solution A is injected in X layer silica gel mould, and the bottom of the gel of the top of the gel that X-1 part mixed solution A is formed and the formation of X part mixed solution A is bonding, and then carry out the demoulding, wherein, 3≤X≤N;
9) repeating step 8), obtain 3-D solid structure, then by step 3) thin polymer film obtaining is wrapping to the surface of 3-D solid structure, after insulation, obtains hydrogel/high molecular polymer thin film muscular tissue support.
2. the manufacture method of hydrogel/high molecular polymer thin film muscular tissue support according to claim 1, is characterized in that,
Step 2) in the concrete steps of prepare silicon sol solution be: get silica gel and the firming agent of certain volume, then firming agent is added in silica gel, obtain silica gel solution, wherein, the volume ratio of silica gel and firming agent is 100: 1.5-2;
Step 2) in resin piece be fixed on silica gel mould by double faced adhesive tape, place 24-28h solidify.
3. the manufacture method of hydrogel/high molecular polymer thin film muscular tissue support according to claim 1, it is characterized in that, step 3) in solvent in PLGA macromolecule polymer solution be deionized water, the mass concentration of PLGA macromolecule polymer solution is 15~20%;
Step 3) in the high molecular polymer PLGA solution preparing is carried out to voltage in the process of electrostatic spinning is 10-15kV, flow velocity is 0.1~0.3ml/min, roller rotating speed is 2800~3600r/min.
4. the manufacture method of hydrogel/high molecular polymer thin film muscular tissue support according to claim 1, is characterized in that,
Step 4) in described N shell silica gel mould is carried out to hydrophilic treated concrete operations be: described N shell silica gel mould is placed into plasma cleaner ionic medium and cleans 2~5min.
5. the manufacture method of hydrogel/high molecular polymer thin film muscular tissue support according to claim 1, it is characterized in that, step 5) in preparation gelatin solution detailed process be: take a certain amount of gelatin, gelatin is poured in the phosphate buffer of certain volume, be to melt 30-40min under the condition of 60-70 DEG C in temperature, stir, obtain gelatin solution, in gelatin solution, the mass concentration of gelatin is 10%~20%.
6. the manufacture method of hydrogel/high molecular polymer thin film muscular tissue support according to claim 5, is characterized in that,
In mixed solution A, the mass concentration of glutamine transaminage is 1~1.2%;
Step 6) in curing hardening time be 4~6min;
Step 6) in step 3) to pass in and out direction consistent for orientation direction and the blood vessel of the thin polymer film that obtains.
7. the manufacture method of hydrogel/high molecular polymer thin film muscular tissue support according to claim 1, is characterized in that, the time that each part of mixed solution A forms gel is 4~6min.
8. the manufacture method of hydrogel/high molecular polymer thin film muscular tissue support according to claim 1, is characterized in that step 9) in the concrete operations of insulation be: 3-D solid structure is placed in 37 DEG C of calorstats and is incubated 4~5h.
CN201410235459.XA 2014-05-29 2014-05-29 The manufacture method of hydrogel/high molecular polymer thin film muscular tissue support Active CN103976805B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201410235459.XA CN103976805B (en) 2014-05-29 2014-05-29 The manufacture method of hydrogel/high molecular polymer thin film muscular tissue support

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201410235459.XA CN103976805B (en) 2014-05-29 2014-05-29 The manufacture method of hydrogel/high molecular polymer thin film muscular tissue support

Publications (2)

Publication Number Publication Date
CN103976805A true CN103976805A (en) 2014-08-13
CN103976805B CN103976805B (en) 2016-03-30

Family

ID=51269144

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201410235459.XA Active CN103976805B (en) 2014-05-29 2014-05-29 The manufacture method of hydrogel/high molecular polymer thin film muscular tissue support

Country Status (1)

Country Link
CN (1) CN103976805B (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104959988A (en) * 2015-05-29 2015-10-07 浙江大学 Muscle driving module based on temperature responsiveness hydrogel
CN107050521A (en) * 2017-04-27 2017-08-18 天新福(北京)医疗器材股份有限公司 A kind of double-layer collagen dermis scaffold and preparation method thereof
CN107151646A (en) * 2017-05-18 2017-09-12 西安交通大学 A kind of active bio battery construction method based on electroctyte
CN107296669A (en) * 2017-05-18 2017-10-27 西安交通大学 A kind of outer suspension holdfast of degradable tracheae and its indirect 3D printing method
CN110344151A (en) * 2019-07-25 2019-10-18 东华大学 Simulate the biomimetic scaffolds and preparation method thereof of natural tendon tissue fiber hierarchical structure

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070009572A1 (en) * 2005-07-06 2007-01-11 Mary Chan Bee E Micro-structured and nano-structured surfaces on biodegradable polymers
US20080208358A1 (en) * 2005-03-07 2008-08-28 Georgia Tech Research Corporation Nanofilament Scaffold for Tissue Regeneration
CN101879097A (en) * 2010-07-06 2010-11-10 西安交通大学 Soft tissue engineering scaffold and preparation process thereof
CN102430151A (en) * 2011-09-05 2012-05-02 西安交通大学 Tissue engineering bone cartilage composite bracket and integrated photocuringable forming method thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080208358A1 (en) * 2005-03-07 2008-08-28 Georgia Tech Research Corporation Nanofilament Scaffold for Tissue Regeneration
US20070009572A1 (en) * 2005-07-06 2007-01-11 Mary Chan Bee E Micro-structured and nano-structured surfaces on biodegradable polymers
CN101879097A (en) * 2010-07-06 2010-11-10 西安交通大学 Soft tissue engineering scaffold and preparation process thereof
CN102430151A (en) * 2011-09-05 2012-05-02 西安交通大学 Tissue engineering bone cartilage composite bracket and integrated photocuringable forming method thereof

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
E. SACHLOS等: "MAKING TISSUE ENGINEERING SCAFFOLDS WORK.REVIEW ON THE APPLICATION OF SOLID FREEFORM FABRICATION TECHNOLOGY TO THE PRODUCTION OF TISSUE ENGINEERING SCAFFOLDS", 《EUROPEAN CELLS AND MATERIALS》 *
SECK TM等: "Designed biodegradable hydrogel structures prepared by stereolithography using poly(ethylene glycol)/poly(D,L-lactide)-based resins", 《JOURNAL OF CONTROL RELEASE》 *
VALERIE LIU TSANG等: "Three-dimensional tissue fabrication", 《ADVANCED DRUG DELIVERY REVIEWS》 *
WANG PY等: "Grooved PLGA films incorporated with RGD/YIGSR peptides for potential application on skeletal muscle tissue engineering", 《COLLOIDS SURFACES B:BIOINTERFACES》 *
连芩等: "面向组织工程化软组织的制造技术及增材制造", 《中国组织工程研究》 *
郁文汉等: "用于三维成型光固化树脂的制备", 《粘接》 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104959988A (en) * 2015-05-29 2015-10-07 浙江大学 Muscle driving module based on temperature responsiveness hydrogel
CN107050521A (en) * 2017-04-27 2017-08-18 天新福(北京)医疗器材股份有限公司 A kind of double-layer collagen dermis scaffold and preparation method thereof
CN107050521B (en) * 2017-04-27 2020-10-09 天新福(北京)医疗器材股份有限公司 Double-layer collagen dermal scaffold and preparation method thereof
CN107151646A (en) * 2017-05-18 2017-09-12 西安交通大学 A kind of active bio battery construction method based on electroctyte
CN107296669A (en) * 2017-05-18 2017-10-27 西安交通大学 A kind of outer suspension holdfast of degradable tracheae and its indirect 3D printing method
CN110344151A (en) * 2019-07-25 2019-10-18 东华大学 Simulate the biomimetic scaffolds and preparation method thereof of natural tendon tissue fiber hierarchical structure

Also Published As

Publication number Publication date
CN103976805B (en) 2016-03-30

Similar Documents

Publication Publication Date Title
CN103976805B (en) The manufacture method of hydrogel/high molecular polymer thin film muscular tissue support
CN102198022B (en) Solid forming method of active cell-hydrogel organ structure
KR101925152B1 (en) Lightweight breast implant material
CN108525012A (en) Gradient hybridization timbering material and preparation method thereof is repaired in a kind of bone cartilage integration
CN104307097B (en) A kind of manufacture method of flexible substrates metal micro-needle array
CN104027847B (en) A kind of manufacture method of artificial soft tissue's body of subsidiary vasoganglion runner
CN105985925B (en) A kind of global function artificial organs fitting body and its preparation and cultural method
KR101661353B1 (en) Preparation method of hydrogel mask pack having supply control function of a single or different skin active ingredients
CN105012050A (en) Method and special mould for preparing tissue and organ precursor with multi-branch channels
CN106730021A (en) A kind of bioactivity glass modified gelatin composite aquogel and preparation method thereof
JP6744008B2 (en) Methods of making structured cellulose patches or elements, and devices made using such methods
CN107714285A (en) A kind of 3 D-printing combination negative-pressure irrigation integration dressing for skin and preparation method thereof
CN110327134A (en) Detachable particular manufacturing craft and the method for preparing multiple-limb channel complex organ precursor
JPH10512176A (en) Porous material products and manufacturing methods
CN107261187A (en) A kind of new microkinetic negative-pressure liquid suction shield traumatic material and preparation method thereof
CN104658395B (en) Heart simulation structure as well as forming method and special mold thereof
CN106075578A (en) A kind of PLGA three-dimensional nerve conduit and preparation method thereof
CN107510884A (en) A kind of Silica hydrogel elasticity scar plaster and preparation method thereof
KR102189616B1 (en) Bioscaffolds for formation of motor endplates and other specialized tissue structures
JP2010516273A (en) Method for treating cultured cells
CN109835099A (en) A method of producing artificial figure head sculpture
CN110124085A (en) A kind of amnion or microlayer model contain combine dressing of drug and preparation method thereof
CN107625970B (en) Acoustic radiation force enhanced inflammatory cell atherosclerosis bionic microbubble complex enrichment and targeting system
CN108690190B (en) Flexible electronic sensor suitable for 3D printed skin and preparation method thereof
CN105771082A (en) Blank pipe fibroin microneedle drug administration system and preparation method thereof

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant