CN102293688A - Silk stent as well as preparation and application thereof - Google Patents
Silk stent as well as preparation and application thereof Download PDFInfo
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- CN102293688A CN102293688A CN2011101498504A CN201110149850A CN102293688A CN 102293688 A CN102293688 A CN 102293688A CN 2011101498504 A CN2011101498504 A CN 2011101498504A CN 201110149850 A CN201110149850 A CN 201110149850A CN 102293688 A CN102293688 A CN 102293688A
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Abstract
The invention discloses a silk stent a preparation method and application to repairing and constructing of organism tissues. The silk stent is mainly composed of a base layer membrane and a water-soluble natural polymer material layer membrane, wherein the base layer membrane is a netting structure woven from silk, and the size of meshes is between 0.25mm<2> and 25mm<2>; and the water-soluble natural polymer material is silk fibroin, hyaluronic acid, collagen or chitosan. The invention has the advantages that: (1) the silk stent has a flat structure, excellent biological and mechanical performances and excellent tissue compatibility; (2) the meshes of the silk stent are uniform in size, and can be regulated; (3) the materials are easily available, and the preparation method is simple and convenient; (4) the natural polymer material adopted in the silk stent has good biological compatibility, and the operation is easy; and (5) the silk stent is suitable for repairing or reinforcing tissues inside an organism, and constructing tissues outside the organism.
Description
(1) technical field
The present invention relates to a kind of organizational project network, particularly a kind of repairing of organism inner tissue or reinforcement, netted silkworm silk support of the outer tissue construction of organism and preparation method thereof of can be used for.
(2) background technology
Tissue defect, weakness are FAQs clinically, and the reason that causes has ectrogeny, pathological factor and aging etc., and clinical manifestation is stomach wall, basin organizational slack, cicatrix and wrinkle etc.Damaged, the weakness of these soft tissues can't be repaired usually voluntarily, have influence on the function and the outward appearance performance of corresponding organ tissue greatly.Particularly because the stress incontinence that the basin relaxes and causes after puerperal or the menopause is the common disease of women.Since the mid-90 in last century, it has become one of the world's 5 big diseases.Mainly adopt the tissue patch repair tissue damaged and weak at present clinically.According to the chemical constituent and the biological property of material, tissue patch can be divided into biological sticking patch and abiotic sticking patch.
The biological sticking patch that comes into the market at present mostly is allosome/xenogeneic tissue products and derivant thereof, though allosome/heteroplasm's wide material sources may cause immunologic rejection and pathophoresis etc., has potential risks.
And present abiotic sticking patch mainly is to comprise some high molecular polymer products.Wherein, the polypropylene sticking patch has been widely applied to clinical, is used for operative treatments such as treatment of urinary incontinence and hernia repairing.But there is the feature that can not absorb and not degrade throughout one's life in this type of sticking patch, it is reported to cause serious recent rejection and etching reaction at a specified future date.
Studies show that silkworm silk is made up of about 75% fibroin and about 25% sericin, it is the native protein that silkworm comes out in the silk glands endocrine, is made up of 18 seed amino acids, mainly is made up of multiple Gly-Ala-Gly-Ala-Ser polyembryony unit.Its avirulence, nonirritant have excellent biological compatibility, so silkworm silk is the more satisfactory raw material of making biomedical material.
Patent 200610051885.3, a kind of netted tissue-engineering stand provides a kind of employing to remove the method for the silkworm silk woven mesh structure of sericin, and its mesh size is 0.5-25mm
2And think that the silkworm silk of removing sericin has good mechanical performance and biocompatibility.Through the silkworm silk network that sericin removal albumen is handled, the phenomenon of crimping takes place easily, the relative out-of-flatness of structure; Influence the result of use of silkworm silk support.Therefore, seek a kind of mechanical property and biology performance modification method to the silkworm silk support, make and a kind ofly can possess flat configuration simultaneously, and excellent mechanical performances and the good medical silkworm silk support that is used for tissue repairing or reinforcement of biocompatibility are arranged, become starting point of the present invention.
(3) summary of the invention
The object of the invention provides a kind of silkworm silk support and preparation method thereof and application, and this silkworm silk support possesses flat configuration, excellent mechanical performances and biocompatibility, can be applicable to the medical support of repairing of organism inner tissue or reinforcement, the outer tissue construction of organism.
The technical solution used in the present invention is:
A kind of silkworm silk support, described silkworm silk support is made of base rack and the water-soluble natural macromolecular material tunic that is coated on the base rack surface, and described base rack is that to be compiled into the mesh size by silkworm silk be 0.25～25mm
2The net chip architecture, described water-soluble natural macromolecular material is natural silk hyaluronic acid, collagen or chitosan.
The preferred described water-soluble natural macromolecular material of the present invention is a natural silk.
Described base rack is preferably worked out by the silkworm silk of removing sericin.
Described silkworm silk support be structure smooth have a mesh-structured lamellar.
Described base rack is made as follows: earlier silkworm silk is removed sericin, and then to use mechanical or manual method braiding to form the mesh size be 0.25～25mm
2The net chip architecture make base rack.
Further, described base rack also can be made as follows: earlier silkworm silk being used mechanical or manual method braiding to form the mesh size is 0.25～25mm
2The net chip architecture for the treatment of sericin, will treat that again the net chip architecture of sericin is removed sericin.
Described silkworm silk goes the method for sericin: it is 0.1%～0.3%Na that silkworm silk or the net chip architecture for the treatment of sericin are immersed in mass concentration
2CO
3In the aqueous solution, boil 60～150min; (2) step (1) is placed pure water through the silkworm silk that boils processing, clean 1～3 time, room temperature or drying baker drying are usually at dry 4～48 hours.
The preparation method of silkworm silk support of the present invention is: it is 0.25～25mm that the silkworm silk that (1) will remove sericin is compiled into the mesh size
2The net chip architecture make base rack; (2) with the surperficial aqueous solution that sprays the water-soluble natural macromolecular material of last layer mass concentration 0.1%～20% of base rack, quantity for spray is 0.5～3.0mL/cm
2, at 20～40 ℃ of drying 4～48h, forming the natural macromolecular material tunic on the base rack surface, cobalt-60 irradiation sterilization is handled, and obtains described silkworm silk support.
Further, the preparation method of described silkworm silk support is preferably carried out according to following steps: it is 0.25～25mm that the silkworm silk that (1) will remove sericin is compiled into the mesh size
2The net chip architecture make base rack; (2) at surface-coated mass concentration 0.1%～20% natural silk aqueous solution of the base rack of step (1) preparation, the amount of the natural polymer of spraying is 0.5～3.0mL/cm
2, 25～40 ℃ of drying 4～48h, cobalt-60 irradiation sterilization obtains described silkworm silk support.
Described silkworm silk support can be applicable to the outer tissue construction of repairing of organism inner tissue or reinforcement and organism.
Described silkworm silk support is preferably applied to treat the medical silkworm silk support of female incontinence.
Basic tunic in the silkworm silk support of the present invention can be to utilize chemistry and physical method to remove the sericin of thick silkworm silk earlier, utilizing mechanical or manual method to be compiled into the net chip architecture, can also utilize mechanical or manual method to be compiled into the net chip architecture earlier, utilize chemistry and physical method to remove the sericin of thick silkworm silk again.
The method master of the thick silk sericin of removal of the present invention Bombyxmori Linnaeus has sodium carbonate liquor to boil the removal method, can also boil the removal method with detergent, boric acid boils the removal method or direct heating is removed method, these methods of removing sericin well-known to those skilled in the art all are fit to the present invention.
The present invention adopts the basis tunic formation of water-soluble natural macromolecular material coating net lamellar silkworm silk to have the good silkworm silk support of flat configuration and mechanics and biology performance, used natural macromolecular material performance: (1) natural silk: it is the main constituent of silkworm silk, its advantage mainly shows, it is single that fibroin applies whole silkworm silk support chemical constituent, the process of heat drying can cause fibroin coated crystal structure to change, and has increased the hardness of silkworm silk support; (2) hyaluronic acid: it is a kind of straight catenate macromolecule polysaccharide, is the hydrophilic molecule of tool of occurring in nature, has the good body compatibility, has the effect of preventing tissue adhesion; (3) collagen: it is the most important water-insoluble fibrin in extracellular, is the skeleton that constitutes extracellular matrix.Collagen forms the fiber of semi-crystal in extracellular matrix, provide tension stress and elasticity to cell, and works in the migration of cell with in growing, and the adding of collagen can promote cell to grow into; (4) chitosan and derivant thereof: they have the hemostasis sterilization, promote functions such as wound healing, are the active materials of a kind of good biological, and the preferred natural silk of the present invention is a natural macromolecular material.
Compared with prior art, beneficial effect of the present invention is mainly reflected in: (1) silkworm silk supporting structure of the present invention is smooth, has both had good biology and mechanical property, has the favorable tissue compatibility again; (2) silkworm silk support of the present invention is formed by the machine braiding, and the mesh size is even, and the mesh size as required can be at 0.25～25mm
2Between adjust; (3) silkworm silk support material therefor of the present invention is easy to get, and preparation method is easy, mainly adopts physical method, can not relate to the introducing of other chemical substances and removes, and has stopped the adverse consequences that chemical method may be brought; (4) natural macromolecular material that is adopted in the silkworm silk support of the present invention, good biocompatibility, simple to operate; (5) silkworm silk support of the present invention is suitable for the repairing of organism inner tissue or reinforcement, the outer tissue construction of organism, can be applicable to treat female incontinence especially.
(4) description of drawings
Silkworm silk surface Electronic Speculum scintigram before and after Fig. 1 silkworm silk sericin removal is handled is before the 1-1 running, after the 1-2 running;
The network structure figure of silkworm silk base rack behind Fig. 2 sericin removal;
The cardinal principle photo of the silkworm silk support after Fig. 3 sprays through the natural silk fibroin;
The cryptoscope photo of the compound silkworm silk support of Fig. 4 bMSCs;
The compound silkworm silk support of Fig. 5 embodiment 8bMSCs is implanted the experiment of animal (rat) urethra, and 4-A is the compound silkworm silk support of bMSCs figure, and 4-B is that the compound silkworm silk support of bMSCs is implanted the experiment of animal urethra;
The compound silkworm silk support of Fig. 6 embodiment 8bMSCs is implanted 4 week of animal (rat) urethra back picture, A schemes substantially for transplanting the compound silkworm silk support of back 4 all bMSCs, B is a cell tracker instrument fluorogram, C is the compound silkworm silk support section of bMSCs picture under laser confocal microscope, and D is CFDA mark fluorescent figure before the compound silkworm silk structural transplantation of bMSCs;
Figure 77-1 is embodiment 8 postoperatives 4 all animal stress incontinence figure, and reaching 7-2 is the LPP result of four groups of laboratory animals, and A is the A group, and B is the B group, and C is the C group, and D is the D group;
Fig. 8 embodiment 12 weeks of 8 postoperatives, the compound silkworm silk internal stent of silkworm silk support and bMSCs structural remodeling figure, A are silkworm silk support HE colored graph, and B is the compound silkworm silk support of a bMSCs HE colored graph, C is a silkworm silk support wheat pine colored graph, and D is the compound silkworm silk support of a bMSCs wheat pine colored graph;
Fig. 9 embodiment 12 weeks of 8 postoperatives, the compound silkworm silk support of silkworm silk support and bMSCs collagen content figure, silk is the silkworm silk groups of holders, silk+bMSC is the compound silkworm silk groups of holders of bMSCs.Vertical coordinate is represented the collagen content of two kinds of different supports;
In Figure 10 embodiment 12 weeks of 8 postoperatives, the compound silkworm silk support of silkworm silk support and bMSCs mechanical property: A is the tension stress experiment, and B is the Young's modulus experiment, and wherein silk is the silkworm silk groups of holders, and silk+bMSC is the compound silkworm silk groups of holders of bMSCs.A group vertical coordinate is represented the tension stress of two kinds of supports, and B group vertical coordinate is represented the Young's modulus of two kinds of supports.
(5) specific embodiment
The present invention is described further below in conjunction with specific embodiment, but protection scope of the present invention is not limited in this:
Embodiment 1:
Thick silkworm silk is compiled into net chip architecture support, and specification is 100mm * 20mm * 0.5mm, and the mesh size is 1.0mm
2, use 0.2%Na then
2CO
3Solution 200mL boils 30min, repeats 3 times, uses the medical purification water rinse of 200mL 3 times, and room temperature (25 ℃) is dried 12h, obtains base rack; Be sprayed on the base rack with 4% silk fibroin water solution, coated weight is 0.5mL/cm
2, dried in the air 24 hours under the room temperature (25 ℃).After the exterior and interior packing,, obtain the silkworm silk support through cobalt-60 irradiation sterilization.
Embodiment 2:
Thick silkworm silk is compiled into the network structure support, and specification is 100mm * 100mm * 0.5mm, and the mesh size is 1.0mm
2, use 0.2%Na then
2CO
3Solution 200mL boils 30min, repeats 3 times.With the medical purification water rinse of 200mL 3 times, 40 ℃ of dry 4h of baking oven, standby.10% chitosan aqueous solution for preparing in advance is sprayed on the silkworm silk support, and coated weight is 1.5mL/cm
2, apply finish after dry 4 hours of 40 ℃ of constant temperature ovens, after the exterior and interior packing,, promptly obtain the silkworm silk support through cobalt-60 irradiation sterilization.
Embodiment 3:
Thick silkworm silk is compiled into the network structure support, and specification is 100mm * 100mm * 0.5mm, and the mesh size is 4.0mm
2, use 0.2%Na then
2CO
3Solution 200mL boils 30min, repeats 3 times.With the medical purification water rinse of 200mL 3 times, room temperature (25 ℃) is dried 24h, and is standby.20% chitosan aqueous solution for preparing in advance is sprayed on the silkworm silk support, and coated weight is 0.5mL/cm
2, apply and finish to do 12 hours through 30 ℃ of constant temperature ovens, after the exterior and interior packing,, promptly obtain the silkworm silk support through cobalt-60 irradiation sterilization.
Embodiment 4:
Thick silkworm silk is compiled into the network structure support, and specification is 50mm * 50mm * 0.5mm, and the mesh size is 0.5mm
2, use 0.2%Na then
2CO
3Solution 200mL boils 30min, repeats 3 times.With the medical purification water rinse of 200mL 3 times, 40 ℃ of dry 6h of baking oven, standby.0.1% hyaluronic acid aqueous solution for preparing in advance is sprayed on the silkworm silk support, and coated weight is 3mL/cm
2Dried in the air 48 hours under the room temperature (25 ℃), after the exterior and interior packing,, promptly obtain the silkworm silk support through cobalt-60 irradiation sterilization.
Embodiment 5:
Thick silkworm silk is compiled into the network structure support, and specification is 50mm * 50mm * 0.5mm, and the mesh size is 0.25mm
2, use 0.2%Na then
2CO
3Solution 200mL boils 30min, repeats 3 times.With the medical purification water rinse of 200mL 3 times, 40 ℃ of dry 8h of baking oven, standby.0.2% collagenic aqueous solution for preparing in advance is sprayed on the silkworm silk support, and coated weight is 3mL/cm
2, did 12 hours through 30 ℃ of constant temperature ovens, after the exterior and interior packing,, promptly obtain the silkworm silk support through cobalt-60 irradiation sterilization.
Embodiment 6
Thick silkworm silk is compiled into the network structure support, and specification is the silkworm silk netting of 60mm * 20mm * 0.5mm, and the mesh size is 1.0mm
2, use 0.2%Na then
2CO
3Solution 200mL boils 30min, repeats 3 times, uses the medical purification water rinse of 200mL 3 times, and room temperature (25 ℃) is dried 12h, obtains base rack, and sem photograph as shown in Figure 1 and Figure 2; Be sprayed on the base rack with 4% silk fibroin water solution, coated weight is 0.5mL/cm
2, dried in the air 24 hours under the room temperature (25 ℃), after the exterior and interior packing,, obtain the silkworm silk support through cobalt-60 irradiation sterilization.The cardinal principle photo of silkworm silk support as shown in Figure 3.
Embodiment 7
Get the bMSCs of the second filial generation, with the low sugar culture medium culturing of serum content 10% after 5-7 days, the high glucose medium of using serum 20% instead continues to cultivate for two weeks, form cell sheet (cell sheet), to prop up according to embodiment 6 acquisition silkworm silks and be placed in the culture dish, with the cell sheet silkworm silk support is rolled in wherein, promptly obtains the compound silkworm silk support of bMSCs.Fig. 4 is shown as the compound silkworm silk support of bMSCs fluorogram.
Embodiment 8: the application of silkworm silk support in female stress urinary
(1) sets up animal model: after intraperitoneal anesthesia, by the cross-section art of bilateral sciatic nerve near-end, do otch from the bilateral skin of back, separate ischiorectal fossa top muscle, expose sciatic nerve 3cm,, set up the urinary incontinence animal model from sciatic nerve initiation excision 2cm, cross-section 4 weeks of postoperative are measured leakage of urine point pressure, and (Leak Point Pressure LPP) confirms as stress incontinence (SUI) model.
(2) the silkworm silk support is used for treatment of urinary incontinence
1) experiment grouping: 40 female Sprague-Dawley rats that grow up are divided into four groups, are respectively: the experiment contrast group: (A group, 5), untreated fish group: (B group, 5), silkworm silk groups of holders: C organizes (15), the compound silkworm silk groups of holders of bMSCs: (D group, 15).
2) stress incontinence: A group is the identical surgical procedure process of process, but the group that do not denervate; B/C/D all handles through the cross-section art of bilateral sciatic nerve near-end for three groups, and in 4 weeks of postoperative, stress test confirms as stress incontinence as shown in Figure 7.
3) treatment of stress incontinence: B group is not carried out the implantation of silkworm silk support; The C group is carried out the silkworm silk support of embodiment 6 preparations and is implanted; The D group is carried out the compound silkworm silk support of bMSCs of embodiment 7 preparations and is implanted, as shown in Figure 5,4 weeks of postoperative, the silkworm silk support as shown in Figure 7, carry out the LPP test and estimate therapeutic effect, the LPP of C group, D group all is significantly higher than the B group near normal value (A group), this shows that the compound silkworm silk support of silkworm silk support and bMSCs can both effectively be treated stress incontinence;
4) tissue repairing ability: after operation 12 weeks of back, the rat urethral tissue is taken out in operation, places 2.5% glutaraldehyde, and 4 ℃ of refrigerators fixedly spend the night, paraffin embedding, section.
HE dyeing: slice, thin piece is placed dimethylbenzene (I) 10min successively, dimethylbenzene (II) 10min, 95% ethanol (I) 10min, 95% ethanol (II) 10min, 80% ethanol 5min, 75% ethanol 5min, dewaxing.Dewaxing is finished and is placed on Lignum Sappan seminal fluid dyeing 10min, and flowing water is flush away Lignum Sappan seminal fluid 10s slightly, and 1% acidic alcohol 3s washes 30s slightly, and short blue liquid returns blue 10s.The 30min of flowing water flushing then.0.5% eosin liquid dyeing 1min, distilled water is washed 2s slightly, and 80% ethanol is washed 2s slightly.Then successively through 95% ethanol (I) 3min, 95% ethanol (II) 3min, dehydrated alcohol 5min, dehydrated alcohol 5min, dimethylbenzene (I) 3min, dimethylbenzene (II) 3min, dimethylbenzene (III) 3min after disposing, uses the neutral gum sealing.
Mai Song dyeing: to brazilwood extract dyeing, tap water washes according to the painted method of HE, acidic alcohol differentiation, tap water flushing; Ponceaux-fuchsin solution is washed 5min, from the beginning washing; The differentiation of 1% phosphomolybdic acid is dyed clear about 5min up to various compositions; 2% pale green liquid dyes 10 minutes (or alternative with 2% aniline blue aqueous solution); 1% aqueous acetic acid is washed 5min, washing; Conventional dehydration, transparent, mounting (with HE dyeing).
By HE dyeing and the dyeing of wheat pine, rebuild from the internal structure of histological observation silkworm silk support implantation group (C group) and the compound silkworm silk support of bMSCs implantation group (D group), result such as Fig. 8, shown in Figure 9, from HE dyeing and wheat pine colored graph as can be seen, the tissue repairing ability of the compound silkworm silk support of D group bMSCs and collagen content all are better than the simple silkworm silk support of C group.
5) mechanical property: after 12 weeks of performing the operation, detect the mechanical property of silkworm silk support implantation group (C group) and the compound silkworm silk support of bMSCs implantation group (D group), respectively silkworm silk support and the compound silkworm silk support of bMSCs are carried out tension stress and Young's modulus test, the test apparatus that is adopted is that (Model 5543, Instron) for the universal electrical Material Testing Machine.In order to guarantee the fresh of sample,, and place 4 ℃ of refrigerators to preserve rapidly with the gauze parcel sample that is soaked with normal saline.Clamped sample keeps the anchor clamps two ends respectively to stay the sample of 5mm.Under the processing of 0.1N pretension, keep 5mm/min to carry out tension test, until sample breakage.
The result as shown in figure 10, the Young's modulus that C organizes simple silkworm silk support is 3.045 ± 0.388Mpa, the Young's modulus of the compound silkworm silk support of D group bMSCs is 4.468 ± 0.510Mpa, and C to organize the tension stress of simple silkworm silk support be 1.521 ± 0.087N, the tension stress of the compound silkworm silk support of D group bMSCs is 2.436 ± 0.192N.Two results show that all the mechanical property of the compound silkworm silk support of D group bMSCs all is better than C and organizes simple silkworm silk support.
Claims (10)
1. silkworm silk support is characterized in that described silkworm silk support is made of base rack and the water-soluble natural macromolecular material tunic that is coated on the base rack surface, and described base rack is that to be compiled into the mesh size by silkworm silk be 0.25～25mm
2The net chip architecture, described water-soluble natural macromolecular material is natural silk, hyaluronic acid, collagen or chitosan.
2. silkworm silk support as claimed in claim 1 is characterized in that described water-soluble natural macromolecular material is a natural silk.
3. silkworm silk support as claimed in claim 1 is characterized in that described base rack worked out by the silkworm silk of removing sericin.
4. silkworm silk support as claimed in claim 1 is characterized in that described silkworm silk support is the smooth lamellar of structure.
5. silkworm silk support as claimed in claim 1 is characterized in that described base rack makes as follows: earlier silkworm silk is removed sericin, and then to use mechanical or manual method braiding to form the mesh size be 0.25～25mm
2The net chip architecture make base rack.
6. silkworm silk support as claimed in claim 1 is characterized in that described base rack makes as follows: earlier silkworm silk being used mechanical or manual method braiding to form the mesh size is 0.25～25mm
2The net chip architecture for the treatment of sericin, the more described net chip architecture of sericin for the treatment of is removed sericin.
7. as claim 3,5,6 described silkworm silk supports, it is characterized in that the described method of sericin of going is: (1) soaks silkworm silk or the net chip architecture for the treatment of sericin in mass concentration is 0.1%～0.3%Na
2CO
3In the aqueous solution, boil 60～150min; (2) silkworm silk that step (1) is boiled after the processing places pure water, cleans 1～3 time, and is dry in room temperature or the drying baker.
8. the preparation method of silkworm silk support as claimed in claim 1 is characterized in that described method makes as follows: it is 0.25～25mm that the silkworm silk that (1) will remove sericin is compiled into the mesh size
2The net chip architecture make base rack; (2) with the aqueous solution of the water-soluble natural macromolecular material of base rack coating quality concentration 0.1%～20%, quantity for spray is 0.5～3.0mL/cm
2, 20～40 ℃ of dryings, forming the natural macromolecular material tunic on the base rack surface, cobalt-60 irradiation sterilization is handled, and obtains described silkworm silk support.
9. the preparation method of silkworm silk support as claimed in claim 1 is characterized in that described preparation method carries out according to following steps: it is 0.25～25mm that the silkworm silk that (1) will remove sericin is compiled into the mesh size
2The net chip architecture make base rack; (2) at surperficial coating quality concentration 0.1%～20% natural silk aqueous solution of the base rack of step (1) preparation, coated weight is 0.5～3.0mL/cm
2, 20～40 ℃ of drying 4～48h, cobalt-60 irradiation sterilization obtains described silkworm silk support.
10. silkworm silk stent applications as claimed in claim 1 is repaired or reinforcement in organism inner tissue, or the outer tissue construction of organism.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102600504A (en) * | 2012-03-20 | 2012-07-25 | 杭州电子科技大学 | Preparation method of mulberry silk tissue engineering scaffold |
| CN104189943A (en) * | 2014-03-30 | 2014-12-10 | 浙江圣兆医药科技有限公司 | Medical composite biomaterial patch and preparation method thereof |
| CN104288840A (en) * | 2014-09-11 | 2015-01-21 | 东华大学 | Porous bioglass/chitosan/silk fibroin composite material and preparation method thereof |
| CN106310373A (en) * | 2015-07-09 | 2017-01-11 | 陕西佰傲再生医学有限公司 | Biological repair membrane and preparation method thereof |
| CN107296981A (en) * | 2017-07-12 | 2017-10-27 | 江苏科技大学 | A kind of fibroin biological support and its preparation method and application |
| CN109758262A (en) * | 2018-12-29 | 2019-05-17 | 浙江星月生物科技股份有限公司 | The adjustable braided silk stent of mechanics degradation property and preparation method and application |
| CN109865159A (en) * | 2018-12-20 | 2019-06-11 | 浙江星月生物科技股份有限公司 | A kind of silk network and its preparation method and application |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050260706A1 (en) * | 2002-06-24 | 2005-11-24 | Tufts University | Silk biomaterials and methods of use thereof |
| US20060153815A1 (en) * | 2004-12-21 | 2006-07-13 | Agnieszka Seyda | Tissue engineering devices for the repair and regeneration of tissue |
| CN1895687A (en) * | 2006-06-09 | 2007-01-17 | 浙江大学 | Netted tissue-engineering stand |
| CN101036802A (en) * | 2006-12-30 | 2007-09-19 | 苏州大学 | Antheraea pernyi silk fibrion biology medicine material and the preparation method |
| EP1997459A1 (en) * | 2007-05-30 | 2008-12-03 | Cordis Corporation | Stent/fibre structural combinations |
| CN101879330A (en) * | 2010-06-02 | 2010-11-10 | 苏州大学 | Small-caliber silk fibroin tubular material and preparation method thereof |
-
2011
- 2011-06-03 CN CN 201110149850 patent/CN102293688B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050260706A1 (en) * | 2002-06-24 | 2005-11-24 | Tufts University | Silk biomaterials and methods of use thereof |
| US20060153815A1 (en) * | 2004-12-21 | 2006-07-13 | Agnieszka Seyda | Tissue engineering devices for the repair and regeneration of tissue |
| CN1895687A (en) * | 2006-06-09 | 2007-01-17 | 浙江大学 | Netted tissue-engineering stand |
| CN101036802A (en) * | 2006-12-30 | 2007-09-19 | 苏州大学 | Antheraea pernyi silk fibrion biology medicine material and the preparation method |
| EP1997459A1 (en) * | 2007-05-30 | 2008-12-03 | Cordis Corporation | Stent/fibre structural combinations |
| CN101879330A (en) * | 2010-06-02 | 2010-11-10 | 苏州大学 | Small-caliber silk fibroin tubular material and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 吴海涛等: "蚕丝在软骨细胞立体培养中的应用", 《中国修复重建外科杂志》, 31 May 2000 (2000-05-31) * |
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