30 years old: O-GlcNAc reaches age of reason - Regulation of cell signaling and metabolism by O-GlcNAcylationTony Lefebvre, Tarik Issad Frontiers Media SA, Jun 10, 2015 - Diseases of the endocrine glands. Clinical endocrinology - 113 pages Hundreds post-translational modifications (PTM) were characterized among which a large variety of glycosylations including O-GlcNAcylation. Since its discovery, O-GlcNAcylation has emerged as an unavoidable PTM widespread in the living beings including animal and plant cells, protists, bacteria and viruses. In opposition to N- and O-glycosylations, O-GlcNAcylation only consists in the transfer of a single N-acetylglucosamine moiety through a beta-linkage onto serine and threonine residues of proteins confined within the cytosol, the nucleus and the mitochondria. The O-GlcNAc group is provided by UDP-GlcNAc, the end-product of the hexosamine biosynthetic pathway located at the crossroad of cell metabolisms making O-GlcNAcylation a PTM which level tightly reflects nutritional status; therefore regulation of cell homeostasis should be intimately correlated to lifestyle and environment. Like phosphorylation, with which it can compete, O-GlcNAcylation is reversible. This versatility is managed by OGT (O-GlcNAc transferase) that transfers the GlcNAc group and OGA (O-GlcNAcase) that removes it. Also, like its unsweetened counterpart, O-GlcNAcylation controls fundamental processes, e.g. protein fate, chromatin topology, DNA demethylation and, as recently revealed, circadian clock. Deregulation of O-GlcNAc dynamism may be involved in the emergence of cancers, neuronal and metabolic disorders such as Alzheimer's or diabetes respectively. |
Contents
OGlcNAc reaches the age of reason regulation of cell signaling and metabolism by OGlcNAcylation | 6 |
Three decades of research on OGlcNAcylation a major nutrient sensor that regulates signaling transcription and cellular metabolism | 8 |
OGlcNAcase expression is sensitive to changes in OGlcNAc homeostasis | 12 |
Proteomic analysis of pig Sus scrofa olfactory soluble proteome reveals OlinkedNacetylglucosaminylation of secreted odorantbinding proteins | 20 |
Global OGlcNAc levels modulate transcription of the adipocyte secretome during chronic insulin resistance | 33 |
Disruption of OGlcNAc cycling in C elegans perturbs nucleotide sugar pools and complex glycans | 45 |
Multiplexed detection of OGlcNAcome phosphoproteome and whole proteome within the same gel | 52 |
OGlcNAcylation and metabolic reprograming in cancer | 62 |
new perspectives in breast and colorectal cancer | 75 |
OGlcNAcylation an epigenetic mark Focus on the histone code TET family proteins and polycomb group proteins | 85 |
a bittersweet switch in liver | 92 |
OGlcNAcylation links ChREBP and FXR to glucosesensing | 99 |
a vast territory to explore | 106 |