In-Cell NMR Spectroscopy: Biomolecular Structure and FunctionAlexander Shekhtman, David S. Burz This Special Issue examines state-of-the-art in-cell NMR spectroscopy as it relates to biological systems of increasing complexity. The compendia of research and recent innovations from prominent laboratories in the field of solid state and solution in-cell NMR spectroscopy, metabolomics and technology development are presented. The work establishes in-cell NMR spectroscopy as the premier method for determining the structures and interaction capabilities of biological molecules at high resolution within the delicately intricate interior of living cells, and the means of utilizing cells as living laboratories to directly assess the effects of exogenous and endogenous stimuli on cell physiology.] |
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acid activity addition amylopectin analysis applications approach assignment authors bacteria binding Biochemistry Biol biology Burz cellular changes Characterization Chem chemical shift coli collected combination complex concentration conformation CrossRef CrossRef][PubMed cytoplasm detection developed domain drug dynamics effects environment example experiments expression Figure folding function glucose human hyperpolarized in-cell NMR spectroscopy in-cell NMR studies increased indicating inside intensity interactions intracellular intrinsically disordered isotopically kinase labeled lipid living cells magnetic mammalian cells measurements membrane method modifications molecular molecules monitoring Nature NMR spectra NMR spectroscopy nuclear observed obtained peaks peptide phosphorylation polarization presence probe processes protein structure PubMed quinary interactions reduced region regulation residues resonance respectively revealed ribosomes samples Selenko Shekhtman showed signal similar solution spectra spectrum starch structure determination target protein transfer types vitro Western blot